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Electro-acupuncture promotes gut motility and alleviates functional constipation by regulating gut microbiota and increasing butyric acid generation in mice

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Journal of Integrative Medicine 2023年第4期21卷 397-406页

作者： ming-min Xu Yu Guo Ying Chen Wei zhang Lu Wang Ying Li School of Acupuncture-Moxibustion and Tuina Chengdu University of Traditional Chinese MedicineChengdu 610075Sichuan ProvinceChina School of Traditional Chinese Medicine Jinan UniversityGuangzhou 510632Guangdong ProvinceChina Office of Educational Administration Chengdu University of Traditional Chinese MedicineChengdu 610075Sichuan ProvinceChina

Objective:Abnormalities in the gut microbiota and intestinal short-chain fatty acid(SCFA)levels are implicated in the pathogenesis of functional constipation(FC).Electro-acupuncture(EA)has been shown to improve constipation-related symptoms and rebalance the gut ***,it is currently unknown whether the gut microbiota is a key mechanistic target for EA or how EA promotes gut motility by regulating the gut microbiota and ***,we assessed the effects of EA in FC mice and pseudo-germfree(PGF)mice to address these ***:Forty female Kunming mice were randomly separated into a normal control group(n=8),an FC group(n=8),an FC+EA group(n=8),a PGF group(n=8)and a PGF+EA group(n=8).The FC group and FC+EA group were treated with diphenoxylate to establish the FC model;the PGF group and PGF+EA group were given an antibiotic cocktail to initiate the PGF *** maintaining the model for 14 d,mice in the FC+EA and PGF+EA groups received EA stimulation at the ST25 and ST37 acupoints,once a day,5 times per week,for 2 *** parameters and intestinal transit rate were calculated to assess the efficacy of EA on constipation and gastrointestinal *** contents were used to quantify gut microbial diversity using 16S rRNA sequencing,and measure SCFA concentrations using gas chromatography-mass ***:EA significantly shortened the first black stool defecation time(Pent(Pent did not reverse slow-transit colonic motility in PGF mice(P>0.05),demonstrating that the gut microbiota may play a mechanistic role in the EA treatment of *** addition,EA treatment restored the Firmicutes to Bacteroidetes ratio and significantly increased butyric acid generation in FC mice(P<0.0

关键词： Electro-acupuncture Functional constipation Gut microbiota Firmicutes/Bacteroidetes ratio Intestinal short-chain fatty acid

亚临界水萃取薄荷黄酮工艺优化及神经保护活性研究

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食品工业科技 2024年第10期45卷 17-24页

作者：张晴晴张梦娇马翊铭蔡学一亳州学院中药学院安徽亳州236800 江南大学化学与材料工程学院江苏无锡214000 安徽雷允上药业有限公司安徽亳州236800

目的:优化亚临界水萃取薄荷黄酮提取工艺,探究薄荷黄酮对H_(2)O_(2)诱导的大鼠肾上腺嗜铬细胞瘤(PC12细胞)损伤的缓解作用。方法:采用单因素实验、Box-Behnken响应面试验优化亚临界水萃取薄荷黄酮工艺,并与回流提取法、超声波辅助提取... 详细信息

目的:优化亚临界水萃取薄荷黄酮提取工艺,探究薄荷黄酮对H_(2)O_(2)诱导的大鼠肾上腺嗜铬细胞瘤(PC12细胞)损伤的缓解作用。方法:采用单因素实验、Box-Behnken响应面试验优化亚临界水萃取薄荷黄酮工艺,并与回流提取法、超声波辅助提取法、超临界CO_(2)萃取法进行比较。体外培养PC12细胞,建立H_(2)O_(2)诱导的PC12细胞损伤模型,噻唑蓝(methyl thiazolye tetrazolium,MTT)法考察薄荷黄酮对PC12细胞存活力的影响,分析神经保护活性。结果:最佳提取工艺为提取温度142℃、提取时间38 min、液料比21:1 mL/g,在此条件下得到薄荷黄酮得率为14.07%±0.23%,比回流提取法提高了334%,比超声波辅助提取法提高了75%,比超临界CO_(2)萃取法提高了173%,显著高于以上3种提取方法的得率(P<0.001)。与H_(2)O_(2)组比较,薄荷黄酮在1~100μg/mL浓度范围内,使PC12细胞存活率从53.61%±0.64%显著(P<0.001)提高到78.49%±0.84%,表明薄荷黄酮能明显缓解细胞氧化损伤。结论:建立了一套绿色、高效、可靠的亚临界水萃取薄荷黄酮的工艺,萃取的薄荷黄酮对H_(2)O_(2)诱导的PC12细胞损伤具有极显著的保护作用(P<0.01)。

关键词：薄荷黄酮亚临界水萃取响应面法细胞损伤神经保护活性工艺优化

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Sustainability of SARS-CoV-2 Induced Humoral Immune Responses in COVID-19 Patients from Hospitalization to Convalescence Over Six Months

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Virologica Sinica 2021年第5期36卷 869-878页

作者： Yang Zheng Qing zhang Ashaq Ali Ke Li Nan Shao Xiaoli Zhou Zhiqin Ye Xiaomin Chen Shanshan Cao Jing Cui Juan Zhou Dianbing Wang Baidong Hou min Li Mengmeng Cui Lihua Deng Xinyi Sun Qian zhang Qinfang Yang Yong li Hui Wang Yake Lei Bo Yu Yegang Cheng Xiaolin Tong Dong Men Xian-en zhang National Laboratory of Biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China Hubei Provincial Hospital of Traditional Chinese Medicine Wuhan 430074China State Key Laboratory of Virology Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China Guang’an Men Hospital China Academy of Chinese Medical SciencesBeijing 100053China Health Bureau of Wuchang District Wuhan 430070China Hubei University Wuhan 430074China Shenzhen mindray Bio-Medical Electronics Co. LtdShenzhen 518057China Hubei Provincial Center for Disease Control&Prevention Wuhan 430079China University of Chinese Academy of Sciences Beijing 100049China

Understanding the persistence of antibody in convalescent COVID-19 patients may help to answer the current major concerns such as the risk of reinfection,the protection period of vaccination and the possibility of building an active herd *** retrospective cohort study included 172 COVID-19 patients who were hospitalized in Wuhan.A total of404 serum samples were obtained over six months from hospitalization to *** in the specimens were quantitatively analyzed by the capture chemiluminescence immunoassays(CLIA).All patients were positive for the anti-SARS-Co V-2 Ig M/Ig G at the onset of COVID-19 symptoms,and the Ig G antibody persisted in all the patients during the ***,only approximately 25%of patients can detect the Ig M antibodies,Ig M against N protein(NIg M)and receptor binding domain of S protein(RBD-Ig M)at the 27 th *** titers of Ig M,N-Ig M and RBD-Ig M reduced to 16.7%,17.6%and 15.2%of their peak values *** contrast,the titers of Ig G,N-Ig G and RBD-Ig G peaked at 4–5 th week and reduced to 85.9%,62.6%and 87.2%of their peak values respectively at the end of *** behavior of antibodies and their correlation in age,gender and severity groups were *** general,the COVID-19 antibody was sustained at high levels for over six months in most of the convalescent *** a few patients with antibody reducing to an undetectable level which needs further *** humoral immune response against SARS-Co V-2 infection in COVID-19 patients exhibits a typical dynamic of acquired immunity.

关键词： Coronavirus disease 2019(COVID-19) Humoral Responses Sustainability Convalescent

基于水声学的阳宗海鱼类行为特征及其资源评估

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南方水产科学 2024年第1期20卷 110-119页

作者：张丽媛杨剑虹熊清海田敏王慧曲品蒋荣明寇春妮武智昆明市水产科学研究所云南昆明650021 中国水产科学研究院珠江水产研究所/农业农村部珠江中下游渔业资源环境科学观测实验站/国家渔业资源环境广州观测实验站广东广州510380 农业农村部外来入侵水生生物防控重点实验室广东广州510380

阳宗海是云南九大湖泊之一,为评估其鱼类分布特征及资源现状,分别于2022年3、5、8和10月采集渔获物,并于6月20—21日昼夜利用鱼探仪进行水声学探测。结果显示,阳宗海共采集鱼类24种,隶属于7目14科23属,太湖新银鱼(Neosalanx taihuensis... 详细信息

阳宗海是云南九大湖泊之一,为评估其鱼类分布特征及资源现状,分别于2022年3、5、8和10月采集渔获物,并于6月20—21日昼夜利用鱼探仪进行水声学探测。结果显示,阳宗海共采集鱼类24种,隶属于7目14科23属,太湖新银鱼(Neosalanx taihuensis)、鲢(Hypophthalmichthys molitrix)、鳙(Aristichthys nobilis)、子陵吻鰕虎(Rhinogobius giurinus)、䱗(Hemiculter leucisculus)、间下鱵(Hyporhamphus intermedius)为阳宗海鱼类优势种。其中,太湖新银鱼、子陵吻鰕虎等小个体鱼类,数量占比超90%。整体来看,鱼类水平空间分布无明显昼夜差异,高密度区均分布在南北2个浅水区。鱼类垂直分布、行为特征存在显著昼夜差异,表现为夜间大量弱声信号聚集于5~10 m水层,白天则分散分布;夜间75%的鱼类向上游动而昼间则相反,中小型鱼类在夜间小幅上浮,而大型鱼类在夜间垂直迁移更为活跃。初步估算,阳宗海鱼类昼夜平均密度分别为0.033和0.038尾·m−3。结果表明,目前阳宗海已形成了以外来鱼类为优势种、土著鱼类种类和数量不断减少的新群落结构。建议加大对太湖新银鱼等小型鱼类的捕捞,继续开展鲢、鳙和中上层土著肉食性鱼类的增殖放流来调整鱼类群落结构,以达到调控水质的目的。

关键词：鱼类资源评估水声学种类组成空间分布昼夜节律阳宗海

Nano-selenium controlled cadmium accumulation and improved photosynthesis in indica rice cultivated in lead and cadmium combined paddy soils

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Journal of environmental Sciences 2021年第5期33卷 336-346页

作者： Chengrun Wang Tingting Cheng Haitao Liu Fangyun Zhou Jifengzhang min zhang Xinya Liu Wenjun Shi Tong Cao School of Biological engineering Huainan Normal UniversityHuainan 232038China Key Laboratory of Bioresource and environmental Biotechnology of Anhui Higher Education Institutes Huainan Normal UniversityHuainan 232038China

Selenium nanoparticles(Se NPs) are less toxic and more biocompatible than selenite or selenate. However, studies involving spraying with Se NPs for reducing accumulation of cadmium(Cd) and lead(Pb) in rice grains have been rarely reported as yet. Herein, indica rice seedlings cultivated in Cd + Pb-spiked paddy soils(denoted as positive control) were sprayed with Se NPs sols for four times from tillering to booting stage. Compared to positive control, 50–100 μmol/L Se NPs downregulated Cd transporters-related genes such as Os LCT 1, OsH MA 2 and Os CCX2 in leaves and OsLCT1, Os PCR1 and Os CCX2 genes in node I at filling stage. Meanwhile, Se-binding protein 1 was distinctly elevated, involving the repression of Cd and Pb transportation to rice grains. Se NPs also differentially improved RuBP carboxylase and chlorophylls especially some key genes and proteins involving photosynthetic system. Besides, 25–50 μmol/L Se NPs diminished reactive oxygen species overproduction from NADPH oxidases whereas boosted glutathione peroxidase, reducing protein carbonylation in rice seedlings. However, the antioxidant isozymes and oxidatively modified proteins were slightly rebounded at 100 μmol/L. Se contents were noticeably elevated and confirmed to exist as selenomethionine in the rice grains following all the treatments by Se NPs. Thus, the optimal dosage of Se NPs for foliar application is 50 μmol/L, which significantly decreased Cd accumulation, improved photosynthesis and Se enrichment whereas caused no distinct reduction of Pb in the grains. Thus, an appropriate dosage of Se NPs can be conducted to decrease Cd accumulation, improve photosynthesis, and organic Se contents in rice grains.

关键词： Selenium nanoparticles(Se NPs) Heavy metals Oryza sativa L. Selenium enrichment and cadmium reduction Food quality and safety

还脑益聪方靶向HAMP调节铁代谢改善AD模型小鼠认知障碍的机制研究

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中国药理学通报 2024年第7期40卷 1240-1248页

作者：孙宁宁贺小平刘珊赵炎钟健民郝雅煊张烨华董贤慧河北中医药大学河北省心脑血管病中医药防治研究重点实验室河北石家庄050091

目的探讨还脑益聪方(Huannao Yicong decoction,HYD)对APP/PS1小鼠学习记忆能力与脑铁代谢的影响以及HAMP基因敲除小鼠(HAMP^(-/-)小鼠)和APP/PS1双转基因模型小鼠的相关性。方法实验分5组,HAMP^(-/-)组:6月龄HAMP基因敲除小鼠;APP/PS1... 详细信息

目的探讨还脑益聪方(Huannao Yicong decoction,HYD)对APP/PS1小鼠学习记忆能力与脑铁代谢的影响以及HAMP基因敲除小鼠(HAMP^(-/-)小鼠)和APP/PS1双转基因模型小鼠的相关性。方法实验分5组,HAMP^(-/-)组:6月龄HAMP基因敲除小鼠;APP/PS1组:6月龄APP/PS1双转基因小鼠;HAMP^(-/-)+还脑益聪方组(HAMP^(-/-)+HYD);APP/PS1+还脑益聪方组(APP/PS1+HYD);阴性对照组:6月龄C57BL/6J小鼠,每组6只。还脑益聪方灌胃给药(还脑益聪方13.68 g·kg^(-1)体质量),其余各组给予蒸馏水灌胃,每天1次,给药2个月。给药结束后,Morris水迷宫测试每组小鼠学习记忆能力;生化法检测各小鼠脑组织铁含量;Western blot与RT-qPCR检测各组小鼠海马转铁蛋白(transferrin,TF)、转铁蛋白受体1(transferrin receptor1,TFR1)、膜铁转运蛋白1(ferroportin1,FPN1)蛋白、二价金属离子转运体1(divalent metal transporter1,DMT1)与β淀粉样蛋白(β-amyloid protein,Aβ)及mRNA表达水平。结果与正常组相比,HAMP^(-/-)组小鼠与APP/PS1组小鼠学习记忆能力均降低,脑组织铁含量升高,HAMP^(-/-)组与APP/PS1组小鼠海马中Aβ蛋白表达升高(P<0.01),TF、TFR1、DMT1蛋白及mRNA表达均升高(P<0.01),FPN1蛋白及mRNA表达降低(P<0.01);分别与HAMP^(-/-)组和APP/PS1组相比,HAMP^(-/-)+HYD组与APP/PS1+HYD组小鼠学习记忆能力均提高,脑组织铁含量降低,Aβ蛋白表达下降(P<0.01),TF、TFR1、DMT1蛋白及mRNA表达降低(P<0.01),FPN1蛋白及mRNA表达升高(P<0.01)。结论HAMP^(-/-)小鼠和APP/PS1小鼠之间具有一定关联性,HYD可以改善HAMP^(-/-)与APP/PS1小鼠学习记忆能力,减少Aβ沉积,其机制可能与调节TF、TFR1、DMT1、FPN1的表达,改善脑铁超载有关。

关键词：还脑益聪方 HAMP基因敲除小鼠铁代谢 APP/PS1 认知障碍 β淀粉样蛋白

基于RNA-Seq筛选高粱低氮胁迫相关候选基因

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作物学报 2024年第3期50卷 669-685页

作者：王瑞张福耀詹鹏杰楚建强晋敏姗赵威军程庆军山西农业大学高粱研究所/高粱遗传与种质创新山西省重点实验室山西晋中030600 西北农林科技大学农学院陕西杨凌712100

研究低氮胁迫条件下不同高粱材料间的基因差异表达,为耐低氮型高粱品种选育和耐低氮胁迫的分子机制探究提供参考。选取2个耐低氮型高粱(BSX44和BTx378)为试验材料,设置正常和低氮胁迫2个处理,利用RNA-Seq技术对高粱苗期、抽穗期和开花... 详细信息

研究低氮胁迫条件下不同高粱材料间的基因差异表达,为耐低氮型高粱品种选育和耐低氮胁迫的分子机制探究提供参考。选取2个耐低氮型高粱(BSX44和BTx378)为试验材料,设置正常和低氮胁迫2个处理,利用RNA-Seq技术对高粱苗期、抽穗期和开花期的基因表达进行分析,通过生物信息学对差异基因的生物学功能和代谢途径进行研究,筛选可能参与低氮调控的基因,了解氮高效基因型在氮素吸收利用过程中可能的分子途径。结果表明,在正常和低氮胁迫下,BTx378和BSX44在苗期分别筛选出937个和787个差异表达基因,抽穗期分别筛选出1305个和935个差异表达基因,开花期分别筛选出1402个和963个差异表达基因。对3个时期的差异表达基因进行鉴定,发现在苗期、抽穗期和开花期分别有246、371和306个基因在2个耐低氮高粱品种中共同差异表达,有28个基因在2个耐低氮品种的不同生育时期均差异表达,其中有5个基因上调表达,23个基因下调表达;对共同差异表达基因的KEGG相关代谢通路富集分析,发现主要集中在氮代谢、丙氨酸,天冬氨酸和谷氨酸代谢、甘油磷脂代谢、氨基酸的生物合成等途径,表明耐低氮型高粱可能通过这些途径相关基因的表达影响其对低氮胁迫的耐受性。

关键词：高粱转录组测序低氮胁迫差异表达基因(DEGs)

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MXene基复合材料在电化学生物传感器检测癌症标志物中的应用进展

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分析化学 2024年第8期52卷 1051-1060页

作者：霍朝晖陈晓雯张晓敏黄晓霞蓝婉莹江琪广东第二师范学院化学与材料科学学院广东省普通高校先进材料与节能减排工程技术开发中心

癌症标志物的检测对于癌症的早期诊断、疗效监测和愈后具有重要意义。现有的传统检测方法存在一些局限性，特别是在癌症早期阶段通常难以及时准确地发现病变。电化学传感器作为一种高效的检测工具，能够满足多类癌症标志物的精准检测需... 详细信息

癌症标志物的检测对于癌症的早期诊断、疗效监测和愈后具有重要意义。现有的传统检测方法存在一些局限性，特别是在癌症早期阶段通常难以及时准确地发现病变。电化学传感器作为一种高效的检测工具，能够满足多类癌症标志物的精准检测需求，为癌症患者的早期确诊提供了有力支持，具有广阔的应用前景。过渡金属碳/氮化物层状材料（MXene）是一种新兴的二维材料，具有高电子转移能力、高导电性、大的比表面积、良好的生物相容性和亲水性以及丰富的表面化学基团等独特的性能，被广泛应用于电化学传感器领域。本文综述了近年来基于MXene基复合材料的电化学生物传感器在癌症标志物检测中的应用进展，分析了MXene在电化学传感器中的作用，讨论了基于MXene的电化学传感器用于癌症早期诊断所面临的挑战和未来的发展趋势。

关键词： MXene基复合材料癌症标志物电化学生物传感器评述

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Ion property and electrical characteristics of 60MHz very-high-frequency magnetron discharge at low pressure

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Plasma Science and Technology 2018年第10期20卷 84-89页

作者： Amin JIANG Chao YE Xiangying WANG min ZHU Su zhang School of Physics Science and Technology Soochow UniversitySuzhou 215006People＇s Republic of China Key Laboratory of Thin Films of Jiangsu Province Soochow UniversitySuzhou 215006People＇s Republic of China Medical College Soochow UniversitySuzhou 215123People＇s Republic of China

The pre-ionized 60 MHz very-high-frequency （VHF） magnetron discharge at low pressure, assisted by inductively coupled plasma （ICP） discharge, was developed. The measurement of ion flux density and ion energy to the substrate was carried out by a retarding field energy analyzer. The electric characteristics of discharge were also investigated by voltage-current probe technique. It was found that by reducing the discharge pressure of VHF magnetron discharge from 5 to 1 Pa, the ion flux density increased about four times, meanwhile the ion energy also increased doubly. The electric characteristics of discharge also showed that a little improvement of sputtering effectiveness was achieved by reducing discharge pressure. Therefore, the deposition property of VHF （60 MHz） magnetron sputtering can be improved by reducing the discharge pressure using the ICP-assisted pre-ionized discharge.

关键词： VHF magnetron sputtering low pressure discharge ion property electric characteristic