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NAT10-mediated ac 4 C-modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear-cell renal cell carcinoma by attenuating YWHAE-driven cytoplasmic retention of YAP1

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Cancer Communications 2024年第3期44卷 361-383页

作者： Daojia Miao Jian Shi Qingyang Lv Diaoyi Tan chuanyi Zhao Zhiyong Xiong Xiaoping zhang department of Urology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubeiP.R.China institute of Urology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubeiP.R.China

Background:Lymphatic metastasis is one of the most common metastatic routes and indicates a poor prognosis in clear-cell renal cell carcinoma(ccRCC).N-acetyltransferase 10(NAT10)is known to catalyze N4-acetylcytidine(ac4C)modification of mRNA and participate in many cellular ***,its role in the lymphangiogenic process of ccRCC has not been *** study aimed to elucidate the role of NAT10 in ccRCC lymphangiogenesis,providing valuable insights into potential therapeutic targets for ***:ac4C modification and NAT10 expression levels in ccRCC were assessed using public databases and clinical *** investigations involved manipulating NAT10 expression in cellular and mouse models to study its role in *** insights were gained through a combination of RNA sequencing,mass spectrometry,co-immunoprecipitation,RNA immuno-precipitation,immunofluorescence,and site-specific mutation ***:We found that ac4C modification and NAT10 expression levels increased in ***10 promoted tumor progression and lymphangiogene-sis of ccRCC by enhancing the nuclear import of Yes1-associated transcriptional regulator(YAP1).Subsequently,we identified ankyrin repeat and zinc fin-ger peptidyl tRNA hydrolase 1(ANKZF1)as the functional target of NAT10,and its upregulation in ccRCC was caused by NAT10-mediated ac4C *** analyses demonstrated that ANKZF1 interacted with tyro-sine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon(YWHAE)to competitively inhibit cytoplasmic retention of YAP1,leading to transcriptional activation of pro-lymphangiogenic ***:These results suggested a pro-cancer role of NAT10-mediated acetylation in ccRCC and identified the NAT10/ANKZF1/YAP1 axis as an under-reported pathway involving tumor progression and lymphangiogenesis in ccRCC.

关键词： clear-cell renal cell carcinoma N4-acetylcytidine N-acetyltransferase 10 YAP1 nuclear import

A novel method to assess antibody-dependent cell-mediated cytotoxicity against influenza A virus M2 in immunized murine models

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Biosafety and Health 2024年第3期6卷 178-185页

作者： Yinjie Liang Junjia Guo Zhen Li Shiyuan Liu Ting zhang Shucai Sun Funa Lu Yuqian Zhai Wenling Wang chuanyi Ning Wenjie Tan Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed by the Province and Ministry Guangxi Medical UniversityNanning 530021China Key Laboratory of Biosafety National Health Commission of the People’s Republic of ChinaNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China School of Laboratory Medicine Wenzhou Medical UniversityWenzhou 325035China School of Public Health Baotou Medical CollegeBaotou 014040China department of Nuclear Medicine The Second Hospital of Hebei Medical UniversityShijiazhuang 050000China department of Microbiology School of Basic MedicineInner Mongolia Medical UniversityHohhot 010110China

The matrix protein 2 (M2) is a preferred target for developing a universal vaccine against the influenza A virus (IAV). This study aimed to develop a method for assessing antibody-dependent cell-mediated cytotoxicity (ADCC) associated with M2-based immunization in mice. We first established a stable cell line derived from mouse lymphoma cells (YAC-1) expressing M2 of H3N2. This cell line, designated as YAC-1-M2, was generated using a second-generation lentiviral tricistronic plasmid system to transduce the M2 gene into YAC-1 cells. The ADCC effect induced by polyclonal antibodies targeting matrix protein 2 ectodomain (M2e) was demonstrated by YAC-1-M2 cell lysis by natural killer cells (NK) derived from mice, in the presence of anti-M2 antibodies obtained from mice immunized with an mRNA vaccine based on M2e. This ADCC effect was found to be stronger compared to the effect induced by monoclonal antibodies (14C2) against M2. Moreover, the ADCC effect was enhanced as the effector-to-target ratio of NK to YAC-1-M2 cells increased. In conclusion, we established a novel method to detect ADCC of M2 of IAV, which paves the way for the development of an M2-based universal vaccine against IAV and an in-depth analysis of its mechanism of broad-spectrum immune protection in mice.

关键词： Antibody-dependent cell-mediated cytotoxicity(ADCC) Influenza A virus(IAV) Matrix protein 2 extracellular domain(M2e) Cell line

Selection and validation of reference genes for quantitative real-time polymerase chain reaction analyses of Serratia ureilytica DW2

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BIOCELL 2023年第3期47卷 647-656页

作者： fenglin BAI BIANXIA BAI TINGTING JIN GUIPING zhang JIAHONG REN The department of Life Sciences Changzhi UniversityChangzhiChina Ecological and Environmental Research institute of Taihang Mountain ChangzhiChina

Background:Serratia ureilytica DW2 is a highly efficient phosphate-solubilizing bacteria isolated from Codonopsis pilosula rhizosphere soil that can promote the growth of ***;nonetheless,until now,no validated reference genes from the genus Serratia have been reported that can be used for the normalization of quantitative real-time polymerase chain reaction(RT–qPCR)***:To screen stable reference genes of *** DW2,the expression of its eight candidate reference genes(16S rRNA,ftsZ,ftsA,mreB,recA,slyD,thiC,and zipA)under different treatment conditions(pH,temperature,culture time,and salt content)was assayed by RT–*** expression stability of these genes was analyzed using different algorithms(geNorm,NormFinder,and BestKeeper).To verify the reliability of the data,the expression of the glucose dehydrogenase(gdh)gene under different soluble phosphate levels was quantified using the most stably expressed reference ***:The results showed that the zipA and 16S rRNA genes were the most stable reference genes,and the least stable genes were thiC and *** expression of gdh was consistent with the phosphate solubilization ability on plates containing the National Botanical Research institute phosphate growth ***:Therefore,this study provides a stable and reliable reference gene of Serratia for the accurate quantification of functional gene expression in future studies.

关键词： Medical plants Codonopsis pilosula Phosphate-solubilizing bacterium Growth-promoting bacteria

N6-methyladenosine-modified DBT alleviates lipid accumulation and inhibits tumor progression in clear cell renal cell carcinoma through the ANXA2/YAP axis-regulated Hippo pathway

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Cancer Communications 2023年第4期43卷 480-502页

作者： Daojia Miao QiWang Jian Shi Qingyang Lv Diaoyi Tan chuanyi Zhao Zhiyong Xiong Xiaoping zhang department of Urology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubeiP.R.China institute of Urology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubeiP.R.China

Background:The mechanism of metabolism reprogramming is an unsolved problem in clear cell renal cell carcinoma(ccRCC).Recently,it was discovered that the Hippo pathway altered tumor metabolism and promoted tumor ***,this study aimed at identifying key regulators of metabolism reprogramming and the Hippo pathway in ccRCC and pinpointing potential therapeutic targets for ccRCC ***:Hippo-related gene sets and metabolic gene sets were used to screen potential regulators of the Hippo pathway in *** databases and samples from patients were applied to investigate the association of dihydrolipoamide branched chain transacylase E2(DBT)with ccRCC and Hippo *** role of DBT was confirmed by gain or loss of function assays in vitro and in *** results were yielded by luciferase reporter assay,immunoprecipitation,mass spectroscopy,and mutational ***:DBT was confirmed as a Hippo-related marker with significant prognostic predictive value,and its downregulationwas caused bymethyltransferaselike-3(METTL3)-mediated N6-methyladenosine(m6A)modification in *** studies specified DBT as a tumor suppressor for inhibiting tumor progression and correcting the lipid metabolism disorder in *** findings revealed that annexin A2(ANXA2)interacted with the lipoyl-binding domain of DBT to activate Hippo signaling which led to decreased nuclear localization of yes1-associated transcriptional regulator(YAP)and transcriptional repression of lipogenic ***:This study demonstrated a tumor-suppressive role for the DBT/ANXA2/YAP axis-regulated Hippo signaling and suggested DBT as a potential target for pharmaceutical intervention in ccRCC.

关键词： clear cell renal cell carcinoma lipid accumulation Hippo signaling N6-methyladenosine dihydrolipoamide branched chain transacylase E2

Development Strategy and Countermeasures of china's CBM Industry under the Goal of “Carbon Peak and Neutrality”

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Journal of Earth Science 2023年第4期34卷 975-984页

作者： Fengyin Xu Xia Yan fenglin Wang Xinyuan Ma Jian Yun Hongna Wang Borui Xu Shuangyuan zhang Delei Mao china United Coalbed Methane National Engineering Research Center Co. Ltd.Beijing 100095China Research institute of Petroleum Exploration&Development PetroChina Coalbed Methane Co.Ltd.Beijing 100028China Research management department CNPC Research Institute of Safety and Environment Technology Co.Ltd.Beijing 102249China Exploration technical R&D Center CNPC Exploration Software Co.Ltd.Beijing 100080China

china’s coalbed methane(CBM) industry is in an extremely important “climbing period” and “strategic opportunity period”,which can be generally characterized by “three low and one small”:low degree of exploration and development,low adaptability of main technology,low return on investment and small development *** the “carbon peak and neutrality” background,the development status of CBM industry is systematically *** resources,technology,management problems and reasons are *** and countermeasures to accelerate the industrial development are put forward according to the factors such as efficient development of resources,major technical breakthrough,talent team training,policy formulation and implementation,return on investment and so *** existing problems are as follows:(a) The overall occurrence conditions in china are complicated and the development is difficult compared with the other countries.(b) The research precision accuracy of CBM resource conditions is not enough.(c) The adaptability of technology is poor.(d) The management mode is not *** view of these problems,this paper puts forward the “two steps” development strategy and the technical and management countermeasures of “five in one”.The corresponding “five in one” technology and management countermeasures are the formulation and implementation of relevant safeguard measures in accordance with the principle of collaborative innovation in five aspects:resources,technology,talents,policies and *** the above measures,the dream and grand blueprint of CBM industry shall be realized.

关键词： coalbed methane CBM industry carbon peak carbon neutrality development strate-gy countermeasures.

基于程函方程与三维速度模型的中国东北地区地幔过渡带接收函数研究

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地球物理学报 2022年第8期65卷 2945-2959页

作者：张炎钮凤林宁杰远北京大学地球与空间科学学院北京100871 美国莱斯大学地球、环境与行星科学系休斯顿770053 中国石油大学石油资源与勘探国家重点实验室和非常规天然气研究所北京102249 河北红山巨厚沉积与地震灾害国家野外科学观测研究站北京100871

本文利用中国东北地区NECESSArray记录到的45505条接收函数,结合该区域最新三维速度模型,采用快速行进法求解程函方程,计算了P-S转换波相对走时,并通过共转换点叠加成像方法获得了该地区下方地幔间断面起伏情况.结果显示,在长白山—五... 详细信息

本文利用中国东北地区NECESSArray记录到的45505条接收函数,结合该区域最新三维速度模型,采用快速行进法求解程函方程,计算了P-S转换波相对走时,并通过共转换点叠加成像方法获得了该地区下方地幔间断面起伏情况.结果显示,在长白山—五大连池连线一带东西约300 km,南北约900 km的狭长形区域内,660-km间断面下陷幅度达20~40 km.其结构可细分为南北两部分,南部更为狭窄且与日本海沟走向一致,对应于从日本海沟俯冲的太平洋板块在地幔过渡带的滞留区;北部对应于从千岛海沟俯冲的太平洋板块在地幔过渡带的滞留区.同时,在长白山西部,存在与长白山—五大连池下陷区形状类似的南北向狭长形抬升区,660-km间断面抬升幅度约为10 km,显示俯冲太平洋板块只延伸至松辽盆地东侧;而在盆地西侧,660-km间断面的下陷区可能与该地区岩石圈拆沉有关.同时,410-km间断面在松辽盆地—渤海一带,以及长白山火山西南和东北方向存在明显的下陷区;另外,在阿巴嘎及阿尔山也有小范围的下陷区.所有这些下陷区,均与地表新生代火山活动区/拉伸区有很好的对应关系,表明这些地表构造与深部热物质上涌有关.

关键词：中国东北接收函数地幔过渡带新生代火山俯冲带

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Improving the protective effects of a FGF for peripheral nerve injury repair using sulfated chitooligosaccharides

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Asian Journal of Pharmaceutical Sciences 2019年第5期14卷 511-520页

作者： Yanmei Liu fenglin Yu Beibei zhang Meng Zhou Yu Bei Yifan zhang Jianzhong Tang Yan yang Yadong Huang Qi Xiang Yueping Zhao Qian Liang yang Liu institute of Biomedicine and Guangdong Provincial Key Laboratory of Bioengineering Medicine Jinan UniversityGuangzhou510632China College of Pharmacy Jinan UniversityGuangzhou510632China department of Pharmacy the First Affiliated Hospital of Zhengzhou UniversityZhengzhou450052China department of Stomatology Jinan University Medical CollegeGuangzhou510632China

Injury to the peripheral nerves can result in temporary or life-long neuronal dysfunction and subsequent economic or social disability. Acidic fibroblast growth factor(a FGF) promotes the growth and survival of neurons and is a possible treatment for peripheral nerve injury. Yet,the actual therapeutic utility of a FGF is limited by its short half-life and instability in vivo. In the present study,we prepared sulfated chitooligosaccharides(SCOS),which have heparinlike properties,to improve the bioactivity of a FGF. We investigated the protective effects of SCOS with or without a FGF on RSC96 cells exposed to Na2 S2 O4 hypoxia/reoxygenation injury. Cell viability was measured by MTT assay and cytotoxicity induced by Na2 S2 O4 was assessed by lactate dehydrogenase(LDH) release into the culture medium. Pretreatment with a FGF and SCOS dramatically decreased LDH release after injury compared to pretreatment with a FGF or SCOS alone. We subsequently prepared an a FGF/SCOS thermo-sensitive hydrogel with poloxamer and examined its effects in vivo. Paw withdrawal thresholds and thermal withdrawal latencies were measured in rats with sciatic nerve injury. Local injection of the a FGF/SCOS hydrogels(a FGF: 40,80 μg/kg) increased the efficiency of sciatic nerve repair compared to a FGF(80 μg/kg) hydrogel alone. Especially a FGF/SCOS thermo-sensitive hydrogel decreased paw withdrawal thresholds from 117.75 ± 8.38(g,4 d) to 65.74 ± 3.39(g,10 d),but a FGF alone group were 140.58 ± 27.54(g,4 d) to 89.12 ± 5.60(g,10 d)(a FGF dose was 80 μg/kg,P < 0.05,n = 8). The thermal withdrawal latencies decreased from 11.61 ± 2.26(s,4 d) to 2.37 ±0.67(s,10 d). However,a FGF alone group were from 17.69 ± 1.47(s,4 d) to4.65 ± 1.73(s,10 d)(P < 0.05,n = 8). Furthermore,the a FGF/SCOS hydrogels also exhibited good biocompatibility in mice. In summary,SCOS improved the protective effects of a FGF in RSC96 cells injured with Na2S2O4 and increased the efficiency of nerve repair and recovery of function

关键词： Acidic fibroblast growth factor Neuroprotection Peripheral nerve injury RSC96 cells Sulfated chitooligosaccharides

Transcriptional landscapes of de novo root regeneration from detached Arabidopsis leaves revealed by time-lapse and single-cell RNA sequencing analyses

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Plant Communications 2022年第4期3卷 83-104页

作者： Wu Liu Yuyun zhang Xing Fang Sorrel Tran Ning Zhai Zhengfei yang Fu Guo Lyuqin Chen Jie Yu Madalene SIson Teng zhang Lijun Sun Hongwu Bian Yijing zhang Li yang Lin Xu National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyChinese Academy of Sciences300 Fenglin RoadShanghai 200032China University of Chinese Academy of Sciences 19A Yuquan RoadBeijing100049China department of Plant Pathology University of GeorgiaAthensGA 30602USA College of Life Sciences Shanghai Normal UniversityShanghai 200234China Hainan institute of Zhejiang University Yazhou Bay Science and Technology CitySanya 572025China School of Life Sciences Nantong UniversityNantongChina institute of Genetic and Regenerative Biology Key Laboratory for Cell and Gene Engineering of Zhejiang ProvinceCollege of Life SciencesZhejiang UniversityHangzhou 310058China State Key Laboratory of Genetic Engineering Collaborative Innovation Center of Genetics and DevelopmentDepartment of BiochemistryInstitute of Plant BiologySchool of Life SciencesFudan UniversityShanghai 200438China These authors contributed equally to this article department of Pharmacology and Chemical Biology UPMC Hillman Cancer CenterMagee-Womens Research InstituteUniversity of PittsburghPittsburghPAUSA

Detached Arabidopsis thaliana leaves can regenerate adventitious roots,providing a platformfor studying de novo root regeneration(DNRR).However,the comprehensive transcriptional framework of DNRR remains ***,we provide a high-resolution landscape of transcriptome reprogramming from wound response to root organogenesis in DNRR and show key factors involved in ***-lapse RNA sequencing(RNA-seq)of the entire leaf within 12 h of leaf detachment revealed rapid activation of jasmonate,ethylene,and reactive oxygen species(ROS)pathways in response *** analyses confirmed that ethylene andROSmay serve as wound signals to ***,time-lapse RNA-seq within 5 d of leaf detachment revealed the activation of genes involved in organogenesis,wound-induced regeneration,and resource allocation in the wounded region of detached leaves during adventitious *** studies showed that BLADE-ON-PETIOLE1/2,which control aboveground organs,PLETHORA3/5/7,which control root organogenesis,and ETHYLENE RESPONSE FACTOR115,which controlswound-induced regeneration,are involved in ***,single-cell RNA-seq data revealed gene expression patterns in thewounded region of detached leaves during adventitious ***,our study not only provides transcriptome tools but also reveals key factors involved in DNRR from detached Arabidopsis leaves.

关键词： single-cell RNA-seq time-lapse RNA-seq de novo root regeneration plant regeneration wounding Arabidopsis thaliana

A biomaterial-based therapy for lower limb ischemia using Sr/Si bioactive hydrogel that inhibits skeletal muscle necrosis and enhances angiogenesis

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Bioactive Materials 2023年第8期26卷 264-278页

作者： Ye Yuan Zhaowenbin zhang Fandi Mo Chen yang Yiren Jiao Enci Wang Yuchong zhang Peng Lin Chengkai Hu Weiguo Fu Jiang Chang Lixin Wang department of Vascular Surgery Zhongshan HospitalFudan University180 Fenglin RoadShanghai200032China department of Vascular Surgery Zhongshan Xiamen HospitalFudan University668 JinhuRoadXiamen361015China Joint Centre of Translational Medicine The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou325000China Zhejiang Engineering Research Center for Tissue Repair Materials Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhou325000China Shanghai institute of Ceramics Chinese Academy of Sciences1295 Dingxi RoadShanghai200050China

Muscle necrosis and angiogenesis are two major challenges in the treatment of lower-limb ischemic *** this study,a triple-functional Sr/Si-containing bioceramic/alginate composite hydrogel with simultaneous bioactivity in enhancing angiogenesis,regulating inflammation,and inhibiting muscle necrosis was designed to treat lower-limb ischemic *** particular,sodium alginate,calcium silicate and strontium carbonate were used to prepare injectable hydrogels,which was gelled within 10 *** importantly,this composite hydrogel sustainedly releases bioactive Sr^(2+)and SiO_(3)^(2-) ions within 28 *** biological activity of the bioactive ions released from the hydrogels was verified on HUVECs,SMCs,C2C12 and Raw 264.7 cells in vitro,and the therapeutic effect of the hydrogel was confirmed using C57BL/6 mouse model of femoral artery ligation in *** results showed that the composite hydrogel stimulated angiogenesis,developed new collateral capillaries,and re-established the blood *** addition,the bioactive hydrogel directly promoted the expression of muscle-regulating factors(MyoG and MyoD)to protect skeletal muscle from necrosis,inhibited M1 polarization,and promoted M2 polarization of macrophages to reduce inflammation,thereby protecting skeletal muscle cells and indirectly promoting *** results indicate that these bioceramic/alginate composite bioactive hydrogels are effective biomaterials for treating hindlimb ischemia and suggest that biomaterial-based approaches may have remarkable potential in treating ischemic diseases.

关键词： Hindlimb ischemic diseases Strontium carbonate and calcium silicate bioceramic Bioactive hydrogel Muscle necrosis Angiogenesis