检索结果-南通市图书馆

Structure-guided manipulation of the regioselectivity of the cyclosporine A hydroxylase CYP-sb21 from Sebekia benihana

引用

Synthetic and Systems Biotechnology 2020年第3期5卷 236-243页

作者： Fengwei Li Li Ma Xingwang zhang Jingfei Chen Feifei Qi Yinyue Huang Zepeng Qu Lishan Yao Wei zhang Eung-Soo Kim Shengying Li State Key Laboratory of Microbial Technology Shandong UniversityQingdaoShandong266237China Shandong Provincial Key Laboratory of Synthetic Biology CAS Key Laboratory of BiofuelsQingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdaoShandong266101China department of Biological Engineering Inha UniversityIncheon22212South Korea Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdaoShandong266237China

The cytochrome P450 enzyme CYP-sb21 from the rare actinomycete Sebekia benihana is capable of hydroxylating the immunosuppressive drug molecule cyclosporine A(CsA)primarily at the 4th N-methyl leucine(MeLeu4),giving rise toγ-hydroxy-N-methyl-L-Leu4-CsA(CsA-4-OH).This oxidative modification of CsA leads to dramatically reduced immunosuppressive activity while retaining the hair growth-promoting side-effect,thus demonstrating great application potential in both pharmaceutical and cosmetic ***,this P450 enzyme also hydroxylates CsA at the unwanted position of the 9th N-methyl leucine(MeLeu9),indicating that the regioselectivity needs to be improved for the development of CsA-4-OH into a commercial hair growth ***,we report the crystal structure of CYP-sb21 in its substrate-free form at 1.85Å.Together with sequence and 3D structure comparisons,Autodock-based substrate docking,molecular dynamics(MD)simulation,and site-directed mutagenesis,we identified a number of key residues including R294,E264,and M179 that can improve catalytic efficiency or change the regioselectivity of CYP-sb21 towards CsA,setting the stage for better enzymatic preparation of *** study also provides new insights into the substrate recognition and binding mechanism of P450 enzymes that accommodate bulky substrates.

关键词： Cytochrome P450 monooxygenase Crystal structure Cyclosporine A Regioselectivity Hair growth stimulator

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

膀胱癌组织中MIS18BP1的表达及临床意义

引用

肿瘤防治研究 2024年第3期51卷 163-168页

作者：曹文静汪煜琳翟雨晴张宗亮原江水宋卫青青岛大学医学部青岛266011 青岛市市立医院检验科青岛266011 大连医科大学研究生院大连116041 青岛大学附属医院泌尿外科青岛266011

目的探讨MIS18BP1在膀胱癌中的表达、与临床病理参数相关性及临床意义。方法TCGA、GEO数据库分析MIS18BP1在肿瘤和对照组中mRNA表达并经qRT-PCR验证;UALCAN在线数据库分析MIS18BP1表达及与临床病理参数、免疫细胞浸润程度相关性;免疫组... 详细信息

目的探讨MIS18BP1在膀胱癌中的表达、与临床病理参数相关性及临床意义。方法TCGA、GEO数据库分析MIS18BP1在肿瘤和对照组中mRNA表达并经qRT-PCR验证;UALCAN在线数据库分析MIS18BP1表达及与临床病理参数、免疫细胞浸润程度相关性;免疫组织化学分析MIS18BP1在膀胱癌中的表达及与临床病理特征的关系;ROC曲线评价MIS18BP1 mRNA对膀胱癌的诊断价值。结果生物信息学分析及qRT-PCR结果显示,与对照组相比较,MIS18BP1 mRNA在膀胱癌中表达增加(P<0.05);免疫组织化学结果表明膀胱癌MIS18BP1蛋白阳性率显著增高(P<0.05),且与肿瘤临床分期、浸润深度及淋巴结转移相关(P<0.05);免疫浸润分析显示MIS18BP1与膀胱癌中免疫细胞浸润相关。结论MIS18BP1基因和蛋白在膀胱癌中表达水平增高,可能通过影响免疫细胞浸润调控膀胱癌的发生发展。

关键词：膀胱癌 MIS18BP1 TCGA数据库 GEO数据库 qRT-PCR 免疫组织化学

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

用于导热绝缘环氧树脂复合材料的氮化硼改性研究进展

引用

山东化工 2024年第3期53卷 64-67,70页

作者：张怀东庞秀江刘源陈利鳄鱼尼卡新材料有限公司安徽亳州236000 青岛科技大学化学与分子工程学院山东青岛266000 青岛科技大学高分子科学与工程学院山东青岛266000

环氧树脂是电子器件常用的绝缘高分子基体材料,但存在导热性偏低的问题。六方氮化硼(h-BN)常被用于环氧树脂的导热填料,而h-BN的化学惰性使其在环氧树脂中的分散性和相容性较差,限制了其作用的发挥,因而对其进行改性就成了导热绝缘环氧... 详细信息

环氧树脂是电子器件常用的绝缘高分子基体材料,但存在导热性偏低的问题。六方氮化硼(h-BN)常被用于环氧树脂的导热填料,而h-BN的化学惰性使其在环氧树脂中的分散性和相容性较差,限制了其作用的发挥,因而对其进行改性就成了导热绝缘环氧树脂复合材料制备中需要面对的一个重要问题。本文主要总结了近年来用于导热绝缘环氧树脂复合材料的氮化硼的改性方法及其特点,其中包括剥离、包覆、场取向和杂化等物理方法,以及功能化、偶联剂修饰、活性剂修饰、化学接枝等化学方法,并对BN改性今后的发展趋势进行了讨论。

关键词：导热率环氧树脂复合材料氮化硼改性

来源：

维普期刊数据库

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

C-doped BiOCl/Bi_(2)S_(3) heterojunction for highly efficient photoelectrochemical detection and photocatalytic reduction of Cr(VI)

引用

Journal of Materials Science & Technology 2023年第33期164卷 188-197页

作者： Chunli Wang Nazhen Liu Xia Zhao Yong Tian Xuwei Chen Yanfeng zhang Liang Fan Baorong Hou Research Center for Analytical Sciences Department of ChemistryCollege of SciencesNortheastern UniversityShenyang 110819China Key Laboratory of Marine Environmental Corrosion and Bio-fouling Institute of OceanologyChinese Academy of SciencesQingdao 266071China School of Environmental & Municipal Engineering Qingdao University of TechnologyQingdao 266033China Intelligent Policing Key Laboratory of Sichuan Province Sichuan Police CollegeLuzhou 646000China

Novel C-BiOCl/Bi_(2)S_(3) composites are prepared by hydrothermal C doping in BiOCl and in-situ growth of Bi_(2)S_(3) on *** with BiOCl,C-BiOCl has a larger exposed surface area and can effectively absorb visible *** construction of a heterojunction in C-BiOCl/Bi_(2)S_(3) further promotes the separation and transfer of photogenerated *** improved photoelectric properties,the optimized 5C-BiOCl/5Bi_(2)S_(3) is applied as a dual-functional composite for photoelectrochemical(PEC)detection and photocatalytic(PC)reduction of Cr(VI).The 5C-BiOCl/5Bi_(2)S_(3) shows a linear range of 0.02-80μM for PEC cathodic detection of Cr(VI)with a detection limit of 0.01628μ***,99.5%of Cr(VI)can be removed via absorption and PC reduction by 5C-BiOCl/5Bi_(2)S_(3),with the reduction rate constant(k)336 times higher than that of BiOCl.

关键词： C-BiOCl/Bi_(2)S_(3) Dual-functional Photoelectrochemical detection Photocatalytic reduction Cr(VI)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioinspired surface functionalization of biodegradable mesoporous silica nanoparticles for enhanced lubrication and drug release

引用

Friction 2023年第7期11卷 1194-1211页

作者： Xiaowei MAO Kexin CHEN Yanlong ZHAO Chunrong Xiong Jing LUO Yuguang WANG Bo WANG Hongyu zhang School of Chemical Engineering and Technology Hainan UniversityHaikou 570228China State Key Laboratory of Tribology Department of Mechanical EngineeringTsinghua UniversityBeijing 100084China Peking university School of Nursing&National Engineering Laboratory for Digital and Material Technology of Stomatology Beijing 100191China Beijing Research Institute of Automation for Machinery Industry Co. Ltd.Beijing 100120China Center of Digital Dentistry Peking University School and Hospital of Stomatology&NHC Research Center of Engineering and Technology for Computerized DentistryBeijing 100081China College of Chemical and Biological Engineering Shandong University of Science and TechnologyQingdao 266590China

Osteoarthritis is associated with the significantly increased friction of the joint,which results in progressive and irreversible damage to the articular cartilage.A synergistic therapy integrating lubrication enhancement and drug delivery is recently proposed for the treatment of early-stage *** the present study,bioinspired by the self-adhesion performance of mussels and super-lubrication property of articular cartilages,a biomimetic self-adhesive dopamine methacrylamide-poly(2-methacryloyloxyethyl phosphorylcholine)(DMA-MPC)copolymer was designed and synthesized via free radical *** copolymer was successfully modified onto the surface of biodegradable mesoporous silica nanoparticles(bMSNs)by the dip-coating method to prepare the dual-functional nanoparticles(bMSNs@DMA-MPC),which were evaluated using a series of surface characterizations including the transmission electron microscope(TEM),Fourier transform infrared(FTIR)spectrum,thermogravimetric analysis(TGA),X-ray photoelectron spectroscopy(XPS),*** tribological test and in vitro drug release test demonstrated that the developed nanoparticles were endowed with improved lubrication performance and achieved the sustained release of an anti-inflammatory drug,i.e.,diclofenac sodium(DS).In addition,the in vitro biodegradation test showed that the nanoparticles were almost completely biodegraded within 10 ***,the dual-functional nanoparticles were biocompatible and effectively reduced the expression levels of two inflammation factors such as interleukin-1β(IL-1β)and interleukin-6(IL-6).In summary,the surface functionalized nanoparticles with improved lubrication and local drug release can be applied as a potential intra-articularly injected biolubricant for synergistic treatment of early-stage osteoarthritis.

关键词： surface functionalization phosphorylcholine coating mesoporous silica nanoparticles hydration lubrication osteoarthritis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Breaking the linear correlations for enhanced electrochemical nitrogen reduction by carbon-encapsulated mixed-valence Fe_(7)(PO_(4))_(6)

引用

Journal of Energy Chemistry 2022年第8期31卷 182-187,I0006页

作者： Lehui Ma Fanfan Xu Linlin zhang Zhongfen Nie Kai Xia Mingxia Guo Mingzhu Li Xin Ding College of Chemistry and Chemical Engineering Qingdao UniversityQingdao 266071ShandongChina

Electrochemical nitrogen reduction(NRR)is deemed as a consummate answer for the traditional Haber–Bosch *** the linear correlations between adsorption and transition-state energies of intermediates is vital to improve the kinetics of ammonia synthesis and obtain a less energy-intensive ***,carbon-encapsulated mixed-valence Fe_(7)(PO_(4))_(6) was prepared and applied as an electrocatalyst for high-efficiency NRR.A dramatic faradaic efficiency(FE)of 36.93%and an NH_(3) production rate of 13.1μg h^(-1) mg_(cat)^(-1) were obtained at-0.3 V versus RHE,superior to nearly all Fe-based *** and DFT calculations revealed that the superior performance was ascribed to the synergistic effect of mixed-valence iron pair,which braked the linear correlations to improve the kinetics of ammonia from collaborative hydrogenation and*NH_(3) *** work proves the feasibility of mixedvalence catalysts for nitrogen reduction and thus opening a new avenue towards artificial nitrogenfixation catalysts.

关键词： N2 reduction reaction(NRR) Mixed-valence Linear correlations Nitrogen fixation N_(2)activation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Integrated energy storage system based on triboelectric nanogenerator in electronic devices

引用

Frontiers of Chemical Science and Engineering 2021年第2期15卷 238-250页

作者： Xiao Feng Yang zhang Le Kang Licheng Wang Chongxiong Duan Kai Yin Jinbo Pang Kai Wang School of Electrical Engineering Qingdao UniversityQingdao 266071China department of Energy“Galileo Ferraris” Politecnico di TorinoTurin 10129Italy School of Materials Science and Engineering Xi’an University of Science and TechnologyXi’an 710054China School of Information Engineering Zhejiang University of TechnologyHangzhou 310023China School of Materials Science and Energy Engineering Foshan UniversityFoshan 528231China Hunan Key Laboratory of Super Microstructure and Ultrafast Process School of Physics and ElectronicsCentral South UniversityChangsha 410012China Collaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong Institute for Advanced Interdisciplinary Research(iAIR)University of JinanJinan 250022China

The emergence of electronic devices has brought earth-shaking changes to people's ***,an extemal power source may become indispensable to the electronie devices due to the limited capacity of *** one of the possible solutions for the extermal power sources,the triboelectric nanogenerator(TENG)provides a novel idea to the increasing mumber of personal electronic *** is a new type of energy ollector,which has become a hot spot in the field of *** is widely used at the acquisition and conversion of mechan-ical enegy to electrice energy through the principle of electrostatic *** this basis,the TENG could be integrated with the energy stonage system into a self-powered system,W hich can supply power to the electronic devices and make them work *** this review,TENG's basic structure as well as its working process and working mode are firstly *** integration method of TENGs with enegy storage systems and the related research status are then introduced in *** the end of this paper,we put forward some problems and discuss the prospect in the future.

关键词： electronic devices triboelectrice nanogenerator mechanical energy sel-powered system

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Comparative Genomics Reveals Evolutionary Drivers of Sessile Life and Left-right Shell Asymmetry in Bivalves

引用

Genomics, Proteomics & Bioinformatics 2022年第6期20卷 1078-1091页

作者： Yang zhang Fan Mao Shu Xiao Haiyan Yu Zhiming Xiang Fei Xu Jun Li Lili Wang Yuanyan Xiong Mengqiu Chen Yongbo Bao Yuewen Deng Quan Huo Lvping zhang Wenguang Liu Xuming Li Haitao Ma Yuehuan zhang Xiyu Mu Min Liu Hongkun Zheng Nai-Kei Wong Ziniu Yu CAS Key Laboratory of Tropical Marine Bio-resources and Ecology and Guangdong Provincial Key Laboratory of applied Marine Biology Innovation Academy of South China Sea Ecology and Environmental EngineeringSouth China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) Guangzhou 511458China Biomarker Technologies Co. LtdBeijing 101301China CAS Key Laboratory of Experimental Marine Biology Center for Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao 266071China State Key Laboratory of Biocontrol College of Life SciencesSun Yat-sen UniversityGuangzhou 510275China Zhejiang Key Laboratory of Aquatic Germplasm Resources College of Biological and Environmental SciencesZhejiang Wanli UniversityNingbo 315100China College of Fisheries Guangdong Ocean UniversityZhanjiang 524088China Hebei Key Laboratory of applied Chemistry College of Environmental and Chemical EngineeringYanshan UniversityQinhuangdao 066044China department of Pharmacology Shantou University Medical CollegeShantou 515041China

Bivalves are species-rich mollusks with prominent protective roles in coastal *** these ancient lineages,colony-founding larvae anchor themselves either by byssus production or by cemented *** latter mode of sessile life is strongly molded by left-right shell asymmetry during larval development of Ostreoida oysters such as Crassostrea ***,we sequenced the genome of *** in high resolution and compared it to reference bivalve genomes to unveil genomic determinants driving cemented attachment and shell ***,loss of the homeobox gene Antennapedia(Antp)and broad expansion of lineagespecific extracellular gene families are implicated in a shift from byssal to cemented attachment in *** transcriptomic analysis shows a conspicuous divergence between leftright asymmetrical *** and symmetrical Pinctada fucata in their expression ***,a couple of orthologous transcription factor genes and lineage-specific shell-related gene families including that encoding tyrosinases are elevated,and may cooperatively govern asymmetrical shell formation in Ostreoida oysters.

关键词： Comparative genomics Ostreoida oyster Attachment Shell asymmetry Bivalve

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

EXISTENCE RESULTS FOR SINGULAR FRACTIONAL p-KIRCHHOFF PROBLEMS

引用

Acta Mathematica Scientia 2022年第3期42卷 1209-1224页

作者： Mingqi XIANG Vicent iu D.RADULESCU Binlin zhang College of Science Civil Aviation University of ChinaTianjin 300300China Faculty of applied mathematics AGH University of Science and Technologyal.Mickiewicza 3030-059 Krak´owPoland department of mathematics University of CraiovaStreet A.I.Cuza No.13200585 CraiovaRomania Institute of mathematics Physics and MechanicsJadranska 191000 LjubljanaSlovenia College of mathematics and Systems Science Shandong University of Science and TechnologyQingdao 266590China

This paper is concerned with the existence and multiplicity of solutions for singular Kirchhoff-type problems involving the fractional p-Laplacian *** precisely,we study the following nonlocal problem:{M (∫∫_(R2N)|x|^(α1p)|y|^(α2p)|u(x) − u(y)|^(p)/|x − y|^(N+ps) dxdy)L_(p)^(s)u = |x| ^(β)f(u) in Ω,u = 0 in R^(N) \ Ω,where L_(p)^(s) is the generalized fractional p-Laplacian operator,N≥1,s∈(0,1),α_(1),α_(2),β∈R,Ω■R^(N) is a bounded domain with Lipschitz boundary,and M:R0^(+)→R0^(+),f:Ω→R are continuous ***,we introduce a variational framework for the above ***,the existence of least energy solutions is obtained by using variational methods,provided that the nonlinear term f has(θ_(p-1))-sublinear growth at ***,the existence of infinitely many solutions is obtained by using Krasnoselskii’s genus ***,we obtain the existence and multiplicity of solutions if f has(θ_(p-1))-superlinear growth at *** main features of our paper are that the Kirchhoff function may vanish at zero and the nonlinearity may be singular.

关键词： Fractional Kirchhoff equation singular problems variational and topological methods

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Highly sensitive color fine-tuning of diblock copolymeric nano-aggregates with tri-metallic cations,Eu(Ⅲ),Tb(Ⅲ),and Zn(Ⅱ),for flexible photoluminescence films(FPFs)

引用

Journal of Materials Science & Technology 2021年第6期65卷 72-81页

作者： Xinzhi Wang Yao Wang Xinbo zhang Wei Ding Longlong Li Linjun Huang Laurence A.Belfiore Jianguo Tang Institute of Hybrid Materials National Center of International Joint Research for Hybrid Materials TechnologyNational Base of International Sci.&Tech.Cooperation on Hybrid MaterialsQingdao UniversityQingdao 266071China department of Chemical and Biological Engineering Colorado State UniversityColorado 80523USA

Luminescence materials have shown promise as display apparatus and lighting *** particularly interesting systems are photoluminescence materials that are capable of reversible colors emitting repeatedly on exposure to *** we report a series of color tunable flexible and transparent photoluminescence films consisting of multi-metals(Eu^(3+),Tb^(3+)and Zn^(3+))induced polymer aggregates(MIPAs)which are distributed uniformly in the polyacrylonitrile(PAN)films without *** have a unique spherical structure due to the self-assembly of polystyrene-block-polyacrylic acid(PS-b-PAA)induced by metal ***,when applied in photoluminescence devices,these photoluminescence films exhibit not only red,green,blue colors(RGB)light,but also other tuned various color light covering the whole visible range upon excitation of 345 nm through adjusting the relative ratios of metal *** the most important key point,non-conductive polymers can be used in photoluminescence devices as host medium,which is not realized in electroluminescent ***,the flexible photoluminescence films(FPFs)innovated herein exhibit the great potential to apply for flexible light-color and light-energy transformation devices.

关键词： Photoluminescence Color tunable Flexible films Polymer aggregates

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：