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Agricultural Sciences in China 2009年第11期8卷 1312-1320页

作者： zhang bian-jiang CHEN Quan-zhan HUA Chun ZHOU Feng ZHOU Quan-chen JIAO De-mao Nanjing Xiaozhuang University Nanjing 211171 P.R. China jiangsu Academy of Agricultural Sciences Nanjing 210014 P.R. China

Aiming to controvert whether the photosynthetic capacity of transgenic rice expressing C4 genes is enhanced, with the C3-type untransformed rice （WT） and maize （a C4 plant） as controls, the activity of C4 photosynthetic enzymes, gas exchange parameters and water use efficiency （WUE） under different light intensities and temperatures, the stable carbon isotope ratio （8-3C） value and the metabolic index of active oxygen as well as plant yield parameters were determined in transgenic rice carrying the PEPC and PPDK genes （CK） in this study. The results showed that the light-saturated photosynthetic rate of CK was intermediate between that of WT and maize, with a slight bias towards that of maize. Under a high light intensity （1 200 μmol m^-2 s^-1） and high temperature （35℃）, CK still exhibited higher photosynthetic capacity, while the Gs decreased. The WUE of CK was only slightly increased, and was similar to that of WT. The δ13C value indicated that CK functioned as a C3 plant. In addition, the tolerance to photo-oxidation and grain yield of CK was enhanced by sprayed with NaHSO3. In conclusion, CK possesses higher photosynthetic productivity under the conditions of high photon flux density （PFD）, high temperature and spraying with NaHSO3 solution, thereby providing a new technical approach and physiological basis for constructing C4-like rice.

关键词： transgenic rice photosynthetic characteristics water use efficiency stomatal conductance

ImSpiRE: image feature-aided spatial resolution enhancement method

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Science China Life Sciences 2025年第1期68卷 272-283页

作者： Yuwei Hua Yizhi zhang Zhenming Guo Shan bian Yong zhang State Key Laboratory of Cardiovascular Diseases and Medical Innovation Center Institute for Regenerative MedicineDepartment of NeurosurgeryShanghai East HospitalShanghai Key Laboratory of Signaling and Disease ResearchFrontier Science Center for Stem Cell ResearchSchool of Life Sciences and TechnologyTongji University

The resolution of most spatially resolved transcriptomic technologies usually cannot attain the single-cell level, limiting their applications in biological discoveries. Here, we introduce ImSpiRE, an image feature-aided spatial resolution enhancement method for in situ capturing spatial transcriptome. Taking the information stored in histological images, ImSpiRE solves an optimal transport problem to redistribute the expression profiles of spots to construct new transcriptional profiles with enhanced resolution, together with extending the gene expression profiles into unmeasured regions. Applications to multiple datasets confirm that ImSpiRE can enhance spatial resolution to the subspot level while contributing to the discovery of tissue domains, signaling communication patterns, and spatiotemporal characterization.

关键词： spatial transcriptome histological images spatial resolution enhancement

转C_4 3基因水稻能量亏缺及其补偿技术探讨

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杂交水稻 2012年第2期27卷 8-10页

作者：张边江南京晓庄学院生物化工与环境工程学院江苏南京211171

转C43基因(PEPC+PPDK+ME)水稻光合速率与转PEPC单基因水稻相近,存在能量亏缺现象。分析了转C43基因水稻能量亏缺的原因,探讨了生产上可用的能量亏缺补偿技术,为转C4基因水稻的推广提供技术途径。

关键词：转基因水稻 C4基因能量专亏缺补偿技术

Effects of carbon content on the microstructure and tensile properties of a low-density steel

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 391-401页

作者： Yongxuan Shang Mingyu Fan Shuyong jiang Zhongwu zhang Key Laboratory of Superlight Materials and Surface Technology Ministry of EducationCollege of Materials Science and Chemical EngineeringHarbin Engineering UniversityHarbin 150001China State Key Laboratory of Metal Material for Marine Equipment and Application Iron&Steel Research Institute of Ansteel Group CorporationAnshan 114009China

Carbon can change the phase components of low-density steels and influence the mechanical *** this study,a new method to control the carbon content and avoid the formation ofδ-ferrite by decarburization treatment was *** microstructural changes and mechanical characteristics with carbon content induced by decarburization were systematically ***-Jaoul(C-J)analysis was employed to examine the work hardening characteristics during the tensile *** decarburization by heat treatments,the carbon content within the austenite phase decreased,while Mn and Al were almost unchanged;this made the steel with full austenite transform into the austenite and ferrite dual ***,(Ti,V)C carbides existed in both matrix phase and the mole fraction almost the *** addition,the formation of other carbides *** loss induced a decrease in strength due to the weakening of the carbon solid *** the steel with the single austinite,the deformation mode of austenite was the dislocation planar glide,resulting in the formation of *** the dual-phase steel,the deformation occurred by the dislocation planar glide of austenite first,with the increase in strain,the cross slip of ferrite took place,forming dislocation cells in *** the late stage of deformation,the work hardening of austinite increased rapidly,while that of ferrite increased slightly.

关键词： low-density steels carbon content decarburization strengthening mechanisms work hardening behavior

Cohort study on the treatment of BRAF V600E mutant metastatic colorectal cancer with integrated Chinese and western medicine

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World Journal of Clinical Oncology 2025年第1期16卷 25-33页

作者： jiang-Yu bian Yu-Fang Feng Wen-Ting He Tong zhang Department of Oncology Xiyuan Hospital of China Academy of Chinese Medical SciencesBeijing 100091China Department of Oncology The Fourth Clinical Medical College of Xinjiang Medical UniversityUrumqi 830001China Department of Oncology Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical UniversityUrumqi 830001China

BACKGROUND Patients with BRAF V600E mutant metastatic colorectal cancer(mCRC)have a low incidence rate,poor biological activity,suboptimal response to conventional treatments,and a poor *** the previous cohort study on mCRC conducted by our team,it was observed that integrated Chinese and Western medicine treatment could significantly prolong the overall survival(OS)of patients with colorectal ***,we further explored the survival benefits in the population with BRAF V600E mutant *** To evaluate the efficacy of integrated Chinese and Western medicine in the treatment of BRAF V600E mutant metastatic colorectal *** A cohort study was conducted on patients with BRAF V600E mutant metastatic colorectal cancer admitted to Xiyuan Hospital of China Academy of Chinese Medical Sciences and Traditional Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region from January 2016 to December *** patients were divided into two *** A total of 34 cases were included,with 23 in Chinese-Western medicine cohort(cohort A)and 11 in Western medicine cohort(cohort B).The median overall survival was 19.9 months in cohort A and 14.2 months in cohort B,with a statistically significant difference(P=0.038,hazard ratio=0.46).The 1-3-year survival rates were 95.65%(22/23),39.13%(9/23),and 26.09%(6/23)in cohort A,and 63.64%(7/11),18.18%(2/11),and 9.09%(1/11)in cohort B,*** analysis showed statistically significant differences in median OS between the two cohorts in the right colon,liver metastasis,chemotherapy,and first-line treatment subgroups(P<0.05).CONCLUSION Integrated Chinese and Western medicine can prolong the survival and reduce the risk of death in patients with BRAF V600E mutant metastatic colorectal cancer,with more pronounced benefits observed in patients with right colon involvement,liver metastasis,combined chemotherapy,and first-line treatment.

关键词： Metastatic colorectal cancer BRAF V600E mutation Integrated Chinese and Western medicine Cohort study

Glycolytic dysregulation in Alzheimer's disease:unveiling new avenues for understanding pathogenesis and improving therapy

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Neural Regeneration Research 2025年第8期20卷 2264-2278页

作者： You Wu Lijie Yang Wanrong jiang Xinyuan zhang Zhaohui Yao Department of Geriatrics Renmin Hospital of Wuhan UniversityWuhanHubei ProvinceChina

Alzheimer's disease poses a significant global health challenge owing to the progressive cognitive decline of patients and absence of curative *** current therapeutic strategies,primarily based on cholinesterase inhibitors and N-methyl-Daspartate receptor antagonists,offer limited symptomatic relief without halting disease progression,highlighting an urgent need for novel research directions that address the key mechanisms underlying Alzheimer's *** studies have provided insights into the critical role of glycolysis,a fundamental energy metabolism pathway in the brain,in the pathogenesis of Alzheimer's *** in glycolytic processes within neurons and glial cells,including microglia,astrocytes,and oligodendrocytes,have been identified as significant contributors to the pathological landscape of Alzheimer's *** changes impact neuronal health and function,thus offering promising targets for therapeutic *** purpose of this review is to consolidate current knowledge on the modifications in glycolysis associated with Alzheimer's disease and explore the mechanisms by which these abnormalities contribute to disease onset and *** focus on the pathways through which glycolytic dysfunction influences Alzheimer's disease pathology should provide insights into potential therapeutic targets and strategies that pave the way for groundbreaking treatments,emphasizing the importance of understanding metabolic processes in the quest for clarification and management of Alzheimer's disease.

关键词： Alzheimer’s disease glial cells glycolysis neuronal metabolism pathogenesis therapeutic targets

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缺氮对不同粳稻品种光合特性的影响

湖北农业科学 2014年第8期53卷 1762-1764,1828页

作者：吴言唐宁张边江南京晓庄学院生物化工与环境工程学院南京211171

为了研究缺氮对不同水稻(Oryza sativa L.)品种光合特性的影响,以9516、武育粳、镇稻8号、Kitaake 4种粳稻为材料,测定氮素缺乏营养液(缺氮处理组)和完全营养液(对照)处理下水稻幼苗的叶绿素含量(以叶绿素SPAD值表示)、根冠比以及水稻... 详细信息

为了研究缺氮对不同水稻(Oryza sativa L.)品种光合特性的影响,以9516、武育粳、镇稻8号、Kitaake 4种粳稻为材料,测定氮素缺乏营养液(缺氮处理组)和完全营养液(对照)处理下水稻幼苗的叶绿素含量(以叶绿素SPAD值表示)、根冠比以及水稻的光合生理相关指标。结果表明,缺氮处理后水稻叶绿素含量、净光合速率、蒸腾速率和气孔导度均低于对照,而根冠比和胞间CO2浓度高于对照,表明缺氮影响了水稻的叶绿素合成,导致水稻的光合速率降低,干物质积累变少,根冠比升高。

关键词：水稻(Oryza sativa L ) 缺氮光合特性

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转基因大豆对我国食用油产业链的影响

湖北农业科学 2012年第4期51卷 649-652页

作者：方刚唐宁张边江南京晓庄学院南京211171

中国是食用油消费大国,又是食用油缺乏的大国,每年需从国外进口食用油约占总量的2/3。评述了转基因大豆的优缺点和我国转基因大豆油的生产消费情况,以及转基因大豆对我国食用油产业链的影响,分析了我国大豆生产与加工的出路,为我国转基... 详细信息

中国是食用油消费大国,又是食用油缺乏的大国,每年需从国外进口食用油约占总量的2/3。评述了转基因大豆的优缺点和我国转基因大豆油的生产消费情况,以及转基因大豆对我国食用油产业链的影响,分析了我国大豆生产与加工的出路,为我国转基因大豆食用油的生产、加工和消费提供了参考。

关键词：转基因大豆食用油产业链

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质粒导入不同种农杆菌冻融法的探讨

湖北农业科学 2007年第3期46卷 329-331页

作者：张边江陈全战南京晓庄学院生命科学系南京210017

利用冻融法,使用不同的CaCl2浓度对不同细胞生长状态的农杆菌进行重组质粒导入的研究,以确定最适的导入条件。结果表明,菌体OD600值和不同的CaCl2浓度对转化有不同程度的影响。在OD600值为0.4时,液氮条件下用浓度为50mmol·L-1的CaCl2... 详细信息

利用冻融法,使用不同的CaCl2浓度对不同细胞生长状态的农杆菌进行重组质粒导入的研究,以确定最适的导入条件。结果表明,菌体OD600值和不同的CaCl2浓度对转化有不同程度的影响。在OD600值为0.4时,液氮条件下用浓度为50mmol·L-1的CaCl2溶液对菌体进行处理得到的转化率最高。实验得到转化子,并通过菌落PCR技术鉴定。

关键词：农杆菌冻融法转化效率 PCR

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丙酸产生菌的筛选、鉴定及培养条件

湖北农业科学 2009年第8期48卷 1856-1859页

作者：李燕波张边江南京晓庄学院生命科学系南京211171

以富含丙酸菌与乳酸菌的奶酪为菌源材料,利用乳酸菌不能以乳酸为碳源这一特点,对丙酸产生菌株进行富集,经过初筛、复筛,从178株菌株中筛选得到5株丙酸产生菌株,其中XZ1号菌株丙酸产量最高且发酵性能稳定,被确定为下一步研究的出发菌株... 详细信息

以富含丙酸菌与乳酸菌的奶酪为菌源材料,利用乳酸菌不能以乳酸为碳源这一特点,对丙酸产生菌株进行富集,经过初筛、复筛,从178株菌株中筛选得到5株丙酸产生菌株,其中XZ1号菌株丙酸产量最高且发酵性能稳定,被确定为下一步研究的出发菌株。对该菌株进行生理生化特性鉴定及16S rDNA全序列分析表明,该菌为费氏丙酸杆菌(Propionibacterium freudenreichii),将其命名为*** XZ1;不同玉米浆浓度及不同碳源对丙酸生产的影响考查表明,玉米浆的最适浓度为40g·L-1,当以20g·L-1葡萄糖及20g·L-1的乳酸为混合碳源时,丙酸产量最高达18.94g·L-1。

关键词：丙酸费氏丙酸杆菌筛选鉴定培养条件