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Expression and Purification of Goose HSP70 and Compound Formation with Virus Polypeptide

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Agricultural Sciences in China 2008年第2期7卷 239-247页

作者： WANG Bao-wei WU Xiao-ping zhang Xu-hui JIA Xiao-hui zhang Ming-ai LONG fang-yu YANG Zhi-gang WANG Lei Water Poultry Graduate School Laiyang Agricultural University Qingdao 266109 P.R.China

The experiment was conducted to study the specific expression of HSP70 caused by heat shock, HSP70 purification and the characteristics of coalescence with antigenic peptide in the formation of the complex. Sixty healthy 6-week-old male Wulong geese were selected and randomly divided into three groups. The control group was slaughtered without heat treatment. Treatment group 1 was shocked with an acute heat treatment at （42 ± 1）℃ for 5 h before they were immediately slaughtered. Treatment group 2 was kept for 12 h after the heat treatment under normal conditions in order to recover and was then slaughtered. Cardiac tissue was taken in order to make paraffin sections for the immunohistochemistry experiment and the liver tissue was used to purify HSP70. The geese heart HSP70 expression differences in the three groups were determined and at the same time the experiments of HSP70 purification and appraisal in the liver tissue were carried on. HSP70 purification and synthesis of HBV PreS1 multi-peptides unified the complex, which was determined by bi-specific antibody enzyme-linked immune sandwich assay. The results indicated that widespread HSP70 positive pellets in the cardiac muscle were found under hot shock conditions. HSP70 expression in the treatment group 1 was centered in the karyotheca and its periphery, but in treatment group 2, it was centered in the surrounding cell membrane. The HSP70 purification could be obtained through two sets of purification plans; both the synthesis peptide and the HSP70 purification form the complex under certain conditions. The double antibody sandwich ELISA technique was applied to detect if the complex had been formed. Positive results showed that the complex was formed. The specific expression of HSP70 under heat shock shifted with time, suggesting that HSP70 possibly had some function in cell protection. High-purity HSP70 protein can be obtained under low-pressure chromatography conditions, and in comparison with each other, it

关键词： heat shock goose HSP70 expression purification appraisal viral multi-peptides complex formation

Impact of admission creatinine level on clinical outcomes of patients with acute ST-elevation myocardial infarction undergoing primary percutaneous coronary intervention with drug-eluting stent implantation

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Chinese Medical Journal 2008年第23期121卷 2379-2383页

作者： zhang Qi zhang Rui-yan SHEN Jie zhang Jian-sheng HU Jian YANG Zheng-kun zhang Xian ZHENG ai-fang SHEN Wei-feng Department of Cardiology Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200025 China

Background Prognosis of patients with acute ST-elevation myocardial infarction （STEMI） and renal dysfunction （RD） who received primary percutaneous coronary intervention （PCI） has not been fully investigated in the drug-eluting stent （DES） era. This study aimed to evaluate the impact of admission serum creatinine level on short-term outcomes in patients with acute STEMI undergoing DES-based primary PCI. Methods Primary PCI with DES implantation was attempted in 619 consecutive STEMI patients within 12 hours of symptom onset. Among them, 86 patients had a serum creatinine level ≥115 μmol/L on admission （RD group）, and the remaining 533 patients had normal renal function （non-RD group）. The primary endpoint was 30-day major adverse cardiac events （MACE, including death, non-fatal reinfarction, and target vessel revascularization）, and the secondary endpoint was subacute stent thrombosis. Results Patients in the RD group were older than those in the non-RD group. There are more female patients in the RD group and they had a history of hypertension, myocardial infarction and revascularization. The occurrence rates of Killip class ≥2 （29.1% vs 18.6%, P=0.02） and multi-vessel （62.8% vs 44.5%, P=0.001） and triple vessel disease （32.6% vs 18.2%, P=0.002）, in-hospital mortality （9.3% vs 3.8%, P=0.03）, and MACE rate during hospitalization （17.4% vs 7.7%, P=-0.006） were higher in the RD group than those in the non-RD group. At a 30-day clinical follow-up, the MACE-free survival rate was significantly reduced in the RD group （76.7% vs 89.9%, P=-0.0003）. Angiographic stent thrombosis occurred in 3 （3.5%） and 7 （1.3%） of patients in the RD group and non-RD group, respectively （P=0.15）. Multivariate analysis revealed that the serum creatinine level 〉115 μmol/L on admission was an independent predictor for MACE rate at a 30-day follow-up （Hazard ratio （HR） 3.31,95% CI 1.19-9.18, P 〈0.001）. Conclusion Despite similar prevalence of stent thrombosis at a 30-day clinical follow-u

关键词： acute myocardial infarction percutaneous coronary intervention drug-eluting stent renal dysfunction prognosis

美国大学学生荣誉承诺制度——内容与意义解读

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比较教育研究 2008年第7期29卷 71-75页

作者：张爱芳浙江大学教育学院浙江杭州310028

荣誉制度是美国大学生对学校的承诺制度,也是一种独特的学生自治制度,其实质是信任学生的道德感和责任感,让学生在学术事务或其他生活事务方面进行自我管理。本文从五个方面对荣誉制度的内容进行了论述:荣誉规则、荣誉誓词、管理机构、... 详细信息

荣誉制度是美国大学生对学校的承诺制度,也是一种独特的学生自治制度,其实质是信任学生的道德感和责任感,让学生在学术事务或其他生活事务方面进行自我管理。本文从五个方面对荣誉制度的内容进行了论述:荣誉规则、荣誉誓词、管理机构、惩罚机制和传统的延续;并对荣誉制度的意义做了简要的分析。

关键词：美国大学荣誉制度学生

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某类特殊拓扑动力系统的部分拓扑弱混合性

纺织高校基础科学学报 2010年第1期23卷 39-41页

作者：张爱芳西北大学数学系陕西西安710127

研究了某类特殊动力系统的弱混合子集的定义.通过给出部分拓扑真弱混合性定义,并加强其条件的方法,得出了部分拓扑真强弱混合性新的概念,且进一步得出了此定义的等价条件.对于某类特殊动力系统的弱混合子集的定义,可以得到它的简化定义.

关键词：弱混合子集部分拓扑弱混合性弱混合真子集部分拓扑真弱混合性强弱混合真子集部分拓扑真强弱混合性

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关于部分拓扑弱混合性与对初值敏感依赖

黑龙江大学自然科学学报 2011年第1期28卷 52-54页

作者：张爱芳西北大学数学系西安710127

研究部分拓扑弱混合性与初值敏感依赖的关系,给出弱混合子集的等价条件。若动力系统满足下述条件:1.A是(X,f)的拓扑弱混合子集;2.X-A是有限集,且f(X-A)X-A;3.=X,则f对初值敏感依赖。在一定的条件下,部分拓扑弱混合性的动力系统是初... 详细信息

研究部分拓扑弱混合性与初值敏感依赖的关系,给出弱混合子集的等价条件。若动力系统满足下述条件:1.A是(X,f)的拓扑弱混合子集;2.X-A是有限集,且f(X-A)X-A;3.=X,则f对初值敏感依赖。在一定的条件下,部分拓扑弱混合性的动力系统是初值敏感依赖的。

关键词：弱混合子集部分拓扑弱混合性对初值敏感依赖

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舒适护理对上消化道出血患者的影响

中国实用护理杂志 2011年第6期27卷 7-8页

作者：章爱芳浙江省安吉县人民医院内二科 313300

目的探讨舒适护理在上消化道出血患者中的应用效果。方法将130例患者随机分为对照组和观察组各65例，对照组采用传统护理模式，观察组进行针对性舒适护理模式，比较2组患者的焦虑情绪和对护理工作的满意度。结果舒适护理干预后观察组的... 详细信息

目的探讨舒适护理在上消化道出血患者中的应用效果。方法将130例患者随机分为对照组和观察组各65例，对照组采用传统护理模式，观察组进行针对性舒适护理模式，比较2组患者的焦虑情绪和对护理工作的满意度。结果舒适护理干预后观察组的焦虑水平明显低于对照组；观察组患者对护理工作满意度明显高于对照组。结论舒适护理对缓解上消化道出血患者的焦虑情绪，提高护理满意度有一定的作用，可增加患者的舒适感与安全感。

关键词：上消化道出血舒适护理应用

The interaction between KIF21A and KANK1 regulates dendritic morphology and synapse plasticity in neurons

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Neural Regeneration Research 2025年第1期20卷 209-223页

作者： Shi-Yan Sun Lingyun Nie Jing zhang Xue fang Hongmei Luo Chuanhai Fu Zhiyi Wei ai-Hui Tang Hefei National Research Center for Physical Sciences at the Microscale CAS Key Laboratory of Brain Function and DiseaseMinistry of Education Key Laboratory for Membrane-less Organelles and Cellular DynamicsDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Institute of Artificial Intelligence Hefei Comprehensive National Science CenterHefeiAnhui ProvinceChina CAS Center for Excellence in Molecular Cell Sciences Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Department of Neurobiology School of Life SciencesSouthern University of Science and TechnologyShenzhenGuangdong ProvinceChina Brain Research Center Southern University of Science and TechnologyShenzhenGuangdong ProvinceChina

Morphological alterations in dendritic spines have been linked to changes in functional communication between neurons that affect learning and ***-4 KIF21A helps organize the microtubule-actin network at the cell cortex by interacting with KANK1;however,whether KIF21A modulates dendritic structure and function in neurons remains *** this study,we found that KIF21A was distributed in a subset of dendritic spines,and that these KIF21A-positive spines were larger and more structurally plastic than KIF21A-negative ***,the interaction between KIF21A and KANK1 was found to be critical for dendritic spine morphogenesis and synaptic *** of either KIF21A or KANK1 inhibited dendritic spine morphogenesis and dendritic branching,and these deficits were fully rescued by coexpressing full-length KIF21A or KANK1,but not by proteins with mutations disrupting direct binding between KIF21A and KANK1 or binding between KANK1 and *** down KIF21A in the hippocampus of rats inhibited the amplitudes of long-term potentiation induced by high-frequency stimulation and negatively impacted the animals’cognitive *** together,our findings demonstrate the function of KIF21A in modulating spine morphology and provide insight into its role in synaptic function.

关键词： actin cytoskeleton dendrite KANK1 KIF21A microtubule spine morphology spine synaptic plasticity talin1

供给侧结构性改革与跨越“中等收入陷阱”——逻辑机理与路径选择

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经济问题 2017年第8期 15-20页

作者：张慧芳艾天霞西安交通大学经济与金融学院陕西西安710061

经过30多年的经济高速增长,2010年中国成功跻身中等偏上收入国家。但2012年以后经济发展却进入新常态时期,面临供需结构错配、人口红利渐消、资源环境瓶颈趋紧、自主创新能力不足等严峻挑战。在全面建成小康社会的重大历史机遇期,以供给... 详细信息

经过30多年的经济高速增长,2010年中国成功跻身中等偏上收入国家。但2012年以后经济发展却进入新常态时期,面临供需结构错配、人口红利渐消、资源环境瓶颈趋紧、自主创新能力不足等严峻挑战。在全面建成小康社会的重大历史机遇期,以供给侧"三大发动机"为主导、需求侧"扩大内需"为辅助,协同推进供给侧结构性改革,既是政府与学界共识、内在要求、实施契机的内在逻辑,也是经济转型升级、行稳致远的客观要求,更是跨越"中等收入陷阱"的必然选择。

关键词：供需双侧结构性改革三大发动机创新驱动新消费

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网络协议的自动化模糊测试漏洞挖掘方法

计算机学报 2011年第2期34卷 242-255页

作者：李伟明张爱芳刘建财李之棠华中科技大学计算机学院武汉430074

随着网络应用越来越复杂和重要,对网络协议的安全性要求也越来越高.模糊测试(Fuzz Testing)作为一种重要的测试手段,通过大量数据的注入来测试网络协议的安全,能够发现拒绝服务、缓冲区溢出和格式化字符串等多种重要漏洞.但是手工进行Fu... 详细信息

随着网络应用越来越复杂和重要,对网络协议的安全性要求也越来越高.模糊测试(Fuzz Testing)作为一种重要的测试手段,通过大量数据的注入来测试网络协议的安全,能够发现拒绝服务、缓冲区溢出和格式化字符串等多种重要漏洞.但是手工进行Fuzz Testing需要精确了解网络协议细节并需要繁重的工作来构造大量测试数据集,导致覆盖率有限,效果也不好.为了解决这个问题,文中结合网络协议逆向工程和Fuzz Testing技术,提出了一种能够自动化识别各种网络协议并产生模糊器进行Fuzz Testing的漏洞挖掘方法.这种方法通过报文分类、多序列比对、特定域识别、模糊器生成多个阶段,自动识别网络协议报文结构并进行Fuzz Testing.经过测试FTP、TNS、EM、ISQLPlus等多种已知和未知网络协议,结果表明这种方法在漏洞挖掘方面效果超过手工分析,并显著提升了测试效率,为提高网络协议的安全性提供了良好的基础,具有重要的应用价值.

关键词：协议逆向工程模糊测试漏洞挖掘