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基于非靶向代谢组学分析甲基化对副干酪乳酪杆菌发酵剂贮藏特性的影响

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食品科学 2025年第1期46卷 49-55页

作者：游明坤张文羿内蒙古农业大学乳品生物技术与工程教育部重点实验室内蒙古呼和浩特010018

副干酪乳酪杆菌(Lacticaseibacillus paracasei)zhang是一株具有调节免疫反应、抗氧化等益生特性的优良菌株。DNA甲基化是一种重要的表观遗传机制,在细菌的很多生理过程中发挥关键作用。为更好利用该菌株,本研究重点关注甲基化对*** Zh... 详细信息

副干酪乳酪杆菌(Lacticaseibacillus paracasei)zhang是一株具有调节免疫反应、抗氧化等益生特性的优良菌株。DNA甲基化是一种重要的表观遗传机制,在细菌的很多生理过程中发挥关键作用。为更好利用该菌株,本研究重点关注甲基化对*** zhang发酵剂贮藏特性的影响。利用*** zhang及其DNA甲基化突变株ΔpglX制备发酵剂并在30℃条件下贮藏90 d。结果表明:与野生型相比,甲基化突变株在贮藏90 d后活菌数极显著提高(Pzhang的贮藏特性。

关键词：副干酪乳酪杆菌 DNA甲基化贮藏特性发酵剂 pglX 非靶向代谢组学

Physiological responses in vitamin C system during hibernation in juvenile Chinese soft-shelled turtle Pelodiscus sinensis

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Journal of Oceanology and Limnology 2019年第2期37卷 767-776,I0001-I0011页

作者： CHEN Bojian NIU Cuijuan YUAN Lin zhang wenyi College of Animal Science and Technology Northwest Agriculture and Forestry University Yangling 712100 China Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering College of Life Sciences Beijing Normal University Beijing 100875 China

Vitamin C (Vc) is an important antioxidant that helps turtles tolerating stressful environment. This work quantified changes in tissue Vc levels during winter hibernation in the soft-shelled turtle Pelodiscus sinensis , to reveal the stress response pattern of tissue Vc during hibernation and contribute basic data for turtle culture. We sampled juvenile soft-shelled turtles at pre-hibernation (17.0°C mud temperature;MT), during hibernation (5.8°C MT) and after arousal (20.1°C MT) in the field. The transcript levels of the gene encoding L-gulonolactone oxidase (GLO), the key enzyme for Vc synthesis, decreased significantly during hibernation. However, GLO activity did not match the GLO transcription patterns and remained stable during hibernation, and showed temperature-dependent kinetic characteristics. Vitamin C levels in the brain, liver, kidney, and spleen (but not muscle) all decreased significantly during hibernation, but recovered to pre-hibernation levels or even higher levels after arousal. The soft-shelled turtle endured 5 months of hibernation with no significant oxidative damage in most tissues, except in the spleen. Splenic Vc was nearly exhausted during hibernation, accompanied by a significantly elevated malonaldehyde (MDA) level. Although the high level of oxidative damage quickly decreased after arousal, the potential tissue damage in the spleen during hibernation might account for the weakened immune capacity of turtles after hibernation.

关键词： Chinese soft-shelled turtle hibernation reactive oxygen species (ROS) L-gulonolactone oxidase oxidative stress

Epidemic-Like Proximity-Based Traffic Offloading

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China Communications 2015年第10期12卷 91-107页

作者： DONG Wenxiang CHEN Jie YANG Ying zhang wenyi Department of Electronic Engineering and Information Science University of Science and Technology of China Key Laboratory of Wireless-Optical Communications Chinese Academy of Sciences

Cellular networks are overloaded due to the mobile traffic surge,and mobile social networks(MSNets) can be leveraged for traffic *** this paper,we study the issue of choosing seed users for maximizing the mobile traffic offloaded from cellular *** introduce a gossip-style social cascade(GSC) model to model the epidemic-like information diffusion process in *** static-case and mobile-case networks,we establish an equivalent view and a temporal mapping of the information diffusion process,*** further prove the submodularity in the information diffusion and propose a greedy algorithm to choose the seed users for traffic offloading,yielding a sub-optimal solution to the NP-hard traffic offloading maximization(TOM) *** are carried out to study the offloading performance,illustrating that the greedy algorithm significantly outperforms the heuristic and random algorithms,and user mobility can help further reduce cellular load.

关键词： cellular traffic offloading graph theory information diffusion mobile social networks proximity-based communication

Effects of acute cold exposure on oxidative balance and total antioxidant capacity in juvenile Chinese soft-shelled turtle,Pelodiscus sinensis

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Integrative Zoology 2017年第5期12卷 371-378页

作者： wenyi zhang Cuijuan NIU Hui JIA Xutong CHEN Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering College of Life SciencesBeijing Normal UniversityBeijingChina

Acute cold exposure may disturb the physiological homeostasis of the body in *** date,there has been no information on the effects of cold exposure on homeostasis of reactive oxygen species(ROS)or antioxidant defense response in the Chinese soft-shelled turtle,Pelodiscus *** this study,*** juveniles were acclimated at 28℃,transferred to 8℃ as cold exposure for 12 h,then moved back to 28℃ rewarming for 24 *** measured the ROS level and total antioxidant capacity(TAC)in the brain,liver,kidney and spleen at 2 and 12 h cold exposure,and at the end of the rewarming ***(MDA)and carbonyl protein were used as markers of oxidative *** being maintained simultaneously at 28℃ were used as the control *** exposure did not disturb the ROS balance in all 4 tissues,while rewarming raised the ROS level in the brain and kidney of *** exposure and rewarming decreased the TAC in the brain,liver and spleen but did not change the TAC in the *** and carbonyl protein levels did not increase during the treatment,indicating no oxidative damage in all 4 tissues of *** results indicated that extreme cold exposure did not impact the inner oxidative balance of ***,but more ROS was produced during *** showed good tolerance to the harsh temperature change through effective protection of its antioxidant defense system to oxidative *** study provides basic data on the stress biology of ***.

关键词： Chinese soft-shelled turtle cold exposure oxidative damage reactive oxygen species total antioxidant capacity

Effects of probiotics and its fermented milk on constipation: a systematic review

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Food Science and Human Wellness 2023年第6期12卷 2124-2134页

作者： Jiaming Yan Meiling Wu Wei Zhao Lai-Yu Kwok wenyi zhang Key Laboratory of Dairy Biotechnology and Engineering Ministry of EducationInner Mongolia Agricultural UniversityHohhot 010018China Key Laboratory of Dairy Products Processing Ministry of AgricultureInner Mongolia Agricultural UniversityHohhot 010018China

This study reviewed clinical evidence of probiotics-mediated effects on *** databases were used in the literature searches,namely Cochrane Library,Embase,Pubmed,and Web of *** last retrieval time was March *** data were analyzed by Review Manager(version 5.3)and Stata(version 14).The primary search retrieved 8418 articles after removing ***,26 studies were included in the *** of probiotics signifi cantly increased stool frequency(MD:1.15 times/week;95%CI:0.59 to 1.70;Pothers(tablets,powder)>fermented milk.

关键词： Constipation Probiotics Fermented milk Meta-analysis Dosage form

A novel advanced oxidation process to degrade organic pollutants in wastewater:Microwave-activated persulfate oxidation

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Journal of Environmental Sciences 2009年第9期21卷 1175-1180页

作者： YANG Shiying WANG Ping YANG Xin WEI Guang zhang wenyi SHAN Liang Key Laboratory of Marine Environment & Ecology Ministry of Education Qingdao 266100 China. College of Environmental Science and Engineering Ocean University of China Qingdao 266100 China. Qingdao Huashan Biochemical Company Limited Qingdao 266216 China

This article, for the first time, provides a novel advanced oxidation process based on sulfate radical （SO^4·-） to degrade organic pollutants in wastewater： microwave （MW）-activated persulfate oxidation （APO） with or without active carbon （AC）. Azo dye acid Orange 7 （AO7） is used as a model compound to investigate the high reactivity of MW-APO. It is found that AO7 （up to 1000 mg/L） is completely decolorized within 5-7 min under an 800 W MW furnace assisted-APO. In the presence of chloride ion （up to 0.50 mol/L）, the decolorization is still 100% completed, though delayed for about 1-2 min. Experiments are made to examine the enhancement by AC. It is exciting to find that the 100% decolorization of AO7 （500 mg/L） is achieved within 3 min by MW-APO using 1.0 g/L AC as catalyst, while the degradation efficiency maintains at 50% by MW energy without persulfate after about 5 min. Besides the destruction of visible light chromophore band of AO7 （484 nm）, during MW-APO, two bands in the ultraviolet region （228 nm and 310 nm） are rapidly broken down. The removal of COD is about 83%-95% for 500 mg/L AO7. SO^4·- is identified with quenching studies using specific alcohols. Both SO^4·- and ·OH could degrade AO7, but SO^4·- plays the dominant role. In a word, MW-APO AC is a new catalytic combustion technology for destruction of organic contamination even for high concentration.

关键词： microwave-activated persulfate oxidation active carbon sulfate radical advanced oxidation technology

Implanting iodine-125 seeds into rat dorsal root ganglion for neuropathic pain: neuronal microdamage without impacting hind limb motion

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Neural Regeneration Research 2014年第12期9卷 1204-1209页

作者： Ling Jiao Tengda zhang Huixing Wang wenyi zhang Saijun Fan Xiaodong Huo Baosen Zheng Wenting Ma Institute of Radiation Medicine Chinese Academy of Medical Sciences Second Affiliated Hospital of Tianjin Medical University

The use of iodine-125 （L251） in cancer treatment has been shown to relieve patients＇ pain. Consid- ering dorsal root ganglia are critical for neural transmission between the peripheral and central nervous systems, we assumed that 125I could be implanted into rat dorsal root ganglia to provide relief for neuropathic pain. 125I seeds with different radioactivity （0, 14.8, 29.6 MBq） were im- planted separately through L4-5 and L5-6 intervertebral foramen into the vicinity of the L5 dorsal root ganglion, von Frey hair results demonstrated the mechanical pain threshold was elevated after implanting 125I seeds from the high radioactivity group. Transmission electron microscopy revealed that nuclear membrane shrinkage, nucleolar margination, widespread mitochondrial swelling, partial vacuolization, lysosome increase, and partial endoplasmic reticulum dilation were visible at 1,440 hours in the low radioactivity group and at 336 hours in the high radio- activity group. Abundant nuclear membrane shrinkage, partial fuzzy nuclear membrane and endoplasmic reticulum necrosis were observed at 1,440 hours in the high radioactivity group. No significant difference in combined behavioral scores was detected between preoperation and postoperation in the low and high radioactivity groups. These results suggested that the mechan- ical pain threshold was elevated after implanting 125I seeds without influencing motor functions of the hind limb, although cell injury was present.

关键词： nerve regeneration iodine-125 analgesia radioactive seeds ultrastructure pain threshold dorsal root ganglion injury neuropathic pain neural regeneration

S-1805/Ag/Au Hybrid Transparent Electrodes for ITO-free Flexible Organic Photovoltaics

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Chemical Research in Chinese Universities 2019年第3期35卷 509-513页

作者： WEN Xuemei YI Fangshun JI Jinhai BI Yangang zhang wenyi WANG Wenquan State Key Laboratory of Integrated Optoelectronics College of Electronic Science and EngineeringJilin UniversityChangchun 130012P.R.China College of Physics Jilin UniversityChangchun 130012P.R.China Systems Engineering Research Institute Beijing 100094P.R.China

We fabricated an S-1805/Ag/Au hybrid transparent electrode based on 4.4 lini ultrathin Au film, which exhibited a high transparency(T=78% at 550 nm), a low sheet resistance(Rs=70Ωl/sq), and an extremely smooth surface(roughness=0.37 nm). The three-dimensional(3D) island growth mode of 4.4 nm Au film was resisted by tlie chemical bonding effect of the nonconductive S-1805 film and the wetting behavior of 0.6 nm Ag layer. The S-1805/Ag/Au hybrid transparent electrodes were used as the replacement of ITO anodes in flexible organic photovoltaics(OPVs), and the S-1805/Ag/Au-based OP Vs resulted in the similar power conversion efficiency compared to ITO-based devices. High flexibility and mechanical robustness were demonstrated in the ITO-free OPVs without obvious degeneration in device performance after over 2000 repeated bending cycles.

关键词： Ultrathin metal film Hybrid transparent electrode Flexible organic photovoltaics

Reduced-search guessing random additive noise decoding of polar codes

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Science China(Information Sciences) 2023年第2期66卷 283-284页

作者： Kefan WANG Yuejun WEI Zhenyuan CHEN Huarui YIN Liping LI wenyi zhang Key Laboratory of Intelligent Computing and Signal Processing of the Ministry of Education of China Anhui University Huawei Technologies Department of Electronic Engineering and Information Science University of Science and Technology of China

Guessing random additive noise decoding(GRAND) has attracted researchers since its introduction in [1]. For an additive noise channel with hard decision output, the algorithm checks all error patterns, and it schedules its search in descending likelihood order. Ordered reliability bit GRAND(ORBGRAND) [2] is a variant of *** soft channel output is available,

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