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Functional diversity of soil microbial communities in response to supplementing 50% of the mineral N fertilizer with organic fertilizer in an oat field

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Journal of Integrative Agriculture 2021年第8期20卷 2255-2264页

作者： zhang mei-jun JIA Ju-qing LU Hua FENG mei-chen YANG Wu-de College of Agriculture Shanxi Agricultural UniversityTaigu 030801P.R.China

The effects of supplementing 50%of the mineral N fertilizer with organic fertilizer on the metabolism and diversity of soil microbial communities in an oat field were investigated using Biolog-Eco *** experiment consisted of five treatments:no fertilizer(CK),mineral N fertilizer applied at 90 and 45 kg ha^(-1) N in the form of urea(U1 and U2,respectively),and U2 supplemented with organic fertilizer in the form of sheep manure at 90 and 45 kg ha^(-1) N(U2OM1 and U2OM2,respectively).Each treatment had three *** experiment was conducted in 2018 and 2019 in Pinglu District,Shanxi Province,*** carbon source utilization by soil microbial communities,such as amino acids,amines,carbohydrates,carboxylic acids,and polymers,increased when 50%of the mineral N fertilizer was replaced with organic fertilizer in both *** result was accompanied by increased richness,dominance,and evenness of the microbial *** utilization of amino acid,amine,and carboxylic acid carbon sources and community evenness were further improved when the organic fertilizer amount was doubled in both *** analysis indicated that amines and amino acids were the most representative of the total carbon source utilization by the soil microbial communities in both *** highest oat yield was achieved at a total N application rate of 135 kg ha^(-1) in the treatment involving 45 kg ha^(-1) N in the form of urea and 90 kg ha^(-1) N in the form of sheep manure in both *** was concluded that the application of 50%of the conventional rate of mineral N fertilizer supplemented with an appropriate rate of organic fertilizer enhanced both the functional diversity of soil microbial communities and oat *** and amino acid carbon sources may be used as a substitute for total carbon sources for assessing total carbon source utilization by soil microbial communities in oat fields in future studies.

关键词： organic fertilizer Biolog-Eco soil microbial community carbon source utilization diversity oats

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燕麦全基因组SSR位点鉴定及多态性标记开发

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生物技术通报 2024年第2期40卷 120-129页

作者：陈凯凌武涛徐逸群高佳张美俊李欣贾举庆山西农业大学农学院太谷030801 山西农业大学杂粮种质资源创新与分子育种国家实验室(筹) 太原030031

【目的】了解燕麦基因组SSR位点分布情况并开发燕麦全基因组SSR标记,为燕麦皮裸基因克隆、遗传图谱构建、群体多样性分析等提供科学的参考依据。【方法】利用已公布的“三分三”裸燕麦参考基因组,通过TBtools软件对燕麦全基因组的SSR位... 详细信息

【目的】了解燕麦基因组SSR位点分布情况并开发燕麦全基因组SSR标记,为燕麦皮裸基因克隆、遗传图谱构建、群体多样性分析等提供科学的参考依据。【方法】利用已公布的“三分三”裸燕麦参考基因组,通过TBtools软件对燕麦全基因组的SSR位点进行检索并利用EXCEL和SPSS软件对检索数据分析其分布特征。根据检索结果设计引物,在构建的F_(2)遗传群体中小规模进行非变性聚丙烯酰胺凝胶电泳验证,并克隆测序其有效性与真实性。【结果】在燕麦基因组中,SSR数量众多,类型丰富。全基因组共检索到828138个SSR位点,SSR平均密度为78.16个/Mb,平均距离为12.90 kb。单、双、三核苷酸重复类型占主导优势。基元重复次数主要集中在5次和6次,A/T、AG/CT和AAC/GTT重复基元为优势基元。燕麦基因组SSR位点呈现中度多态性,长度变化范围为11-1587 bp。并由此开发设计52对多态性引物在试验材料中进行扩增验证,在构建的遗传群体中均具有多态性。【结论】在“三分三”裸燕麦参考基因组中开发SSR引物有效可行,可以用于后续进一步试验研究。

关键词：燕麦皮裸 SSR标记引物开发多态性

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Editorial

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Science China(Physics,Mechanics & Astronomy) 2009年第11期52卷 1637-1638页

作者： Hongqi zhang, mei zhang, jun Lin, Pengfei Chen & Cheng Fang On behalf of the Editorial Board of this issue

On August 1, 2008 a total solar eclipse was visible within a narrow corridor that traversed from North America to China. The path of the Moon’s umbral shadow started from Canada and extended

关键词： Editorial zhang

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Association of glutathione peroxidase-1 （GPx-1） rs1050450 Pro198Leu and Pro197Leu polymorphisms with cardiovascular risk：a meta-analysis of ob-servational studies

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Journal of Geriatric Cardiology 2014年第2期11卷 141-150页

作者： jun-Xia zhang Zhi-mei WANG jun-Jie zhang Lin-Lin ZHU Xiao-Fei GAO Shao-Liang CHEN Department of Cardiology Nanjing First Hospital Affiliated to Nanjing Medical University Nanjing Jiangsu Province 210006 China

Objective To clarify the association between rs1050450 polymorphism in Glutathione peroxidase-1 （GPx-1） and the risk of cardi-ovascular diseases （CVD） by performing a meta-analysis of published studies. There is growing evidence from different study types for an association of the GPx-1 polymorphism and cardiovascular outcomes, but observational studies have so far shown inconsistent results. Me-thods Relevant publications were searched through PubMed, Embase database databases and the Cochrane Library. We used odds ratios （ORs） with 95%confidence intervals （CIs） to assess the strength of association under the best genetic model. Both Q statistic and the I2 were used to check heterogeneity. Meta-regression analysis was performed to explore heterogeneity source. Sensitivity analysis, cumulative me-ta-analysis analysis and publication bias were used to test the reliability of the results. Results Data were available from two cohort studies and 8 case-control studies involving 1,430 cases and 3,767 controls. The pooled ORs for overall CVD risk was 1.36 with 95%CI：1.08-1.70 under a co-dominant model, and that for East Asian subgroup was 1.84 （95%CI：1.39-2.43）. Substantial heterogeneity for ORs were de-tected among all the included studies, mainly caused by ethnic differences between East Asian and non-East Asian populations. Although Egger＇s regression test suggested no statistical significant publication bias, Begg＇s funnel plot exhibited obvious asymmetry. The statistical significance disappeared after adjusting for potential publication bias in the overall studies. However, no substantial publication bias was found in the East Asian subgroup. Conclusions GPx-1 gene Pro198Leu and Pro197Leu polymorphisms considerably increased the risk of CVD in the East Asian population. Large-scale investigations are needed to confirm the results in different ethnicities.

关键词： Glutathione peroxidase-1 Cardiovascular diseases Polymorphism Meta-analysis

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煤矸石动态淋滤中重金属污染物的释放规律研究

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四川环境 2024年第2期43卷 185-190页

作者：黄蕾刘伟殷良俊黄剑峰张俊梅新疆工程学院化学与环境工程学院乌鲁木齐830091

为探究煤矸石在雨水冲刷作用下重金属元素的释放规律及特征,以新疆某采矿区煤矸石为研究对象,采用动态模拟淋滤实验,研究煤矸石中Fe、Mn、Zn和Cu元素的释放规律。结果表明,Fe、Mn、Zn和Cu元素在淋滤前期溶出速率较快,淋出浓度较高,之后... 详细信息

为探究煤矸石在雨水冲刷作用下重金属元素的释放规律及特征,以新疆某采矿区煤矸石为研究对象,采用动态模拟淋滤实验,研究煤矸石中Fe、Mn、Zn和Cu元素的释放规律。结果表明,Fe、Mn、Zn和Cu元素在淋滤前期溶出速率较快,淋出浓度较高,之后随着淋滤时间的延长,各金属元素溶出速率降低,淋出浓度减小,最后趋于稳定,释放达到平衡,整体表现为快速-慢速-平稳释放特征。结合地下水质量标准的要求和各重金属元素在淋滤期内的最大淋滤浓度,该矸石所在地的地下水适用于农业和部分工业用水;金属元素在淋滤期内(8d)的淋出值大小表现为Fe>Mn>Zn>Cu的顺序,Fe元素有相对较强的淋出活性,有向土壤和地下水迁移的潜在风险;矸石中重金属元素的淋滤释放呈指数曲线规律衰减,根据衰减模型可定量预测矸石中重金属在动态淋滤时对土壤和地下水的污染强度。本次实验结果为建立煤矸石堆放条件提供了数据支撑,并为煤矸石堆放场周围水体和土壤的污染治理提供了重要的科学依据。

关键词：煤矸石重金属动态淋滤淋滤浓度衰减模型污染强度

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Contribution of Root Respiration to Total Soil Respiration in a Betula ermanii-Dark Coniferous Forest Ecotone of the Changbai Mountains, China

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Pedosphere 2005年第4期15卷 448-455页

作者： LIU Ying HAN Shi-Jie ZHOU Yu-mei zhang jun-Hui Institute of Applied Ecology Chinese Academy of Sciences Shenyang 110016 （China）.

Total and root-severed soil respiration rates for five plots set up 50 m apart in a Betula ermanii Cham.-dark coniferous forest ecotone on a north-facing slope of the Changbai Mountains, China, were measured to evaluate the seasonal variations of soil respiration, to assess the effect of soil temperature and water content on soil respiration, and to estimate the relative contributions of root respiration to the total soil respiration. PVC cylinders in each of 5 forest types of a B. ermanii-dark coniferous forest ecotone were used to measure soil respirations both inside and outside of the cylinders. The contribution of roots to the total soil respiration rates ranged from 12.5% to 54.6%. The mean contribution of roots for the different plots varied with the season, increasing from 32.5% on june 26 to 36.6% on August 3 and to 41.8% on October 14. In addition, there existed a significant (P < 0.01) logarithmic relationship between total soil respiration rate and soil temperature at 5 cm soil depth. Also, a similar trend was observed for the soil respiration and soil water content at the surface (0-5 cm) during the same period of time.

关键词： Betula ermanii Cham. dark coniferous forest ecotone root respiration soil respiration

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ICP-AES标准加入法同时测定钼粉中的多种杂质元素

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机械工程与自动化 2024年第3期 105-107页

作者：张俊美李辉姚佳人董园园栾雪张昕中国兵器工业第五二研究所烟台分所有限责任公司山东烟台264003 上海材料研究所有限公司上海市工程材料应用与评价重点实验室上海200437

研究并建立了ICP-AES标准加入法同时测定钼粉中Fe、Ni、Al、Cu、Ca和Mg等杂质元素含量的方法。该方法采用盐酸-硝酸溶解样品,以标准加入法配制系列标准工作溶液;待测元素的分析谱线分别为Fe238.204 nm、Ni231.604 nm、Al167.078 nm、Cu3... 详细信息

研究并建立了ICP-AES标准加入法同时测定钼粉中Fe、Ni、Al、Cu、Ca和Mg等杂质元素含量的方法。该方法采用盐酸-硝酸溶解样品,以标准加入法配制系列标准工作溶液;待测元素的分析谱线分别为Fe238.204 nm、Ni231.604 nm、Al167.078 nm、Cu327.396 nm、Mg280.270 nm、Ca393.366 nm;线性相关系数均达到0.996以上;测定结果的相对标准偏差为1.97%~5.08%,加标回收率为88%~113%;测定结果与国家标准GB/T 4325方法测定结果比对,基本相吻合。该方法测定结果准确可靠,能够满足科研生产对钼粉中Fe、Ni、Al、Cu、Ca和Mg等杂质元素含量测定的需求。

关键词：钼粉 ICP-AES 标准加入法

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基于CT评估胸肌指数对胸腔镜食管癌根治术后短期预后的影响

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中国CT和MRI杂志 2024年第2期22卷 82-84页

作者：张君梅孙喆马晓苏裴仁明安徽省第二人民医院安徽合肥230041

目的探讨术前基于CT评估胸肌指数(PMI)对胸腔镜食管癌根治术后短期预后的影响。方法选择安徽省第二人民医院胸心外科2018年12月至2022年2月因食管癌接受胸腔镜根治性手术患者80例为研究对象,根据PMI得分中位数将患者分为高PMI组和低PMI... 详细信息

目的探讨术前基于CT评估胸肌指数(PMI)对胸腔镜食管癌根治术后短期预后的影响。方法选择安徽省第二人民医院胸心外科2018年12月至2022年2月因食管癌接受胸腔镜根治性手术患者80例为研究对象,根据PMI得分中位数将患者分为高PMI组和低PMI组。患者在入院后48h内通过电子病历系统收集性别、年龄、体质量指数、肿瘤位置、TNM分期、白蛋白、淋巴细胞计数情况。统计患者无进展生存(PFS)和总生存(OS);术后7d内使用营养风险筛查评分2002(NRS2002)量表对患者营养风险进行筛查,统计患者住院时间、住院期间肺部感染情况。结果两组性别、年龄、体质量指数、肿瘤位置、TNM分期和淋巴细胞计数比较差异无统计学意义(P>0.05);低PMI组白蛋白低于高PMI组,差异具有统计学意义(P<0.05);80例患者共随访13~40个月,中位随访27个月,共16例死亡,其中高PMI组5例,低PMI组11例。高PMI组中位PFS、OS分别为23个月和29个月,低PMI组PFS、OS分别为15个月和24个月,两组比较差异具有统计学意义(χ^(2)=8.100、5.049,P=0.004、0.025);COX回归分析结果显示,矫正前后术前PMI均是食管癌患者短期预后的独立影响因素(P<0.05)。低PMI组营养风险、住院时间和肺部感染发生率高于高PMI组,比较差异有统计学意义(P<0.05)。结论低PMI的胸腔镜食管癌根治术患者术后短期病情进展和死亡风险较高、营养风险较高,住院时间较长,肺部感染发生率也较高。

关键词：胸肌指数胸腔镜食管癌根治术短期预后营养风险

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Feature Matching via Topology-Aware Graph Interaction Model

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IEEE/CAA Journal of Automatica Sinica 2024年第1期11卷 113-130页

作者： Yifan Lu Jiayi Ma Xiaoguang mei jun Huang Xiao-Ping zhang the Electronic Information School Wuhan UniversityWuhan 430072China IEEE Tsinghua Berkeley Shenzhen Institute Shenzhen 518071China the Department of Electrical Computer and Biomedical EngineeringToronto Metropolitan UniversityToronto ON M5B 2K3Canada

Feature matching plays a key role in computer vision. However, due to the limitations of the descriptors, the putative matches are inevitably contaminated by massive *** paper attempts to tackle the outlier filtering problem from two aspects. First, a robust and efficient graph interaction model,is proposed, with the assumption that matches are correlated with each other rather than independently distributed. To this end, we construct a graph based on the local relationships of matches and formulate the outlier filtering task as a binary labeling energy minimization problem, where the pairwise term encodes the interaction between matches. We further show that this formulation can be solved globally by graph cut algorithm. Our new formulation always improves the performance of previous localitybased method without noticeable deterioration in processing time,adding a few milliseconds. Second, to construct a better graph structure, a robust and geometrically meaningful topology-aware relationship is developed to capture the topology relationship between matches. The two components in sum lead to topology interaction matching(TIM), an effective and efficient method for outlier filtering. Extensive experiments on several large and diverse datasets for multiple vision tasks including general feature matching, as well as relative pose estimation, homography and fundamental matrix estimation, loop-closure detection, and multi-modal image matching, demonstrate that our TIM is more competitive than current state-of-the-art methods, in terms of generality, efficiency, and effectiveness. The source code is publicly available at http://***/YifanLu2000/TIM.

关键词： Feature matching graph cut outlier filtering topology preserving

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气相色谱法测定氟苯尼考中二乙胺和三乙胺残留

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精细化工中间体 2024年第3期54卷 84-88页

作者：谭跃浪胡克斌韦晶晶陈蕾张俊梅张春侠吴寒梅普洛药业股份有限公司浙江东阳322118 浙江普洛得邦制药有限公司浙江东阳322118

建立气相色谱法测定氟苯尼考中二乙胺和三乙胺残留的方法。采用Inert Cap for Amines毛细管胺类测试柱,经惰性处理的衬管,载气为氮气,流速3.0 mL/min,分流比为5∶1,检测器为FID。结果表明:该方法中二乙胺、三乙胺与其相邻峰完全分离;对... 详细信息

建立气相色谱法测定氟苯尼考中二乙胺和三乙胺残留的方法。采用Inert Cap for Amines毛细管胺类测试柱,经惰性处理的衬管,载气为氮气,流速3.0 mL/min,分流比为5∶1,检测器为FID。结果表明:该方法中二乙胺、三乙胺与其相邻峰完全分离;对照峰面积RSD均小于2%;二乙胺的检测限浓度为1.84μg/mL,定量限浓度为3.67μg/mL,在3.67~13.76μg/mL浓度范围内线性相关系数r为0.995 9;三乙胺的检测限浓度为0.50μg/mL,定量限浓度为1.50μg/mL,在1.50~374.63μg/mL浓度范围内线性相关系数r为0.998 6;二乙胺的回收率90.7%~103.0%,三乙胺的回收率90.8%~97.3%;小幅改变色谱条件,耐用性良好。方法操作简单、准确度高、重复性好,适用于氟苯尼考中二乙胺、三乙胺残留的测定。

关键词：氟苯尼考二乙胺三乙胺 Inert Cap for Amines胺类测试柱

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