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Molecular Analysis and Expression patterns of Four 14-3-3 Genes from Brassica napus L.

agricultural sciences in china 2010年第7期9卷 942-950页

作者： zhan gao-miao,tong jin,wang han-zhong and hua wei key laboratory for biological sciences of oil crops,ministry of agriculture/oil crops research institute,chinese academy of agricultural sciences,wuhan 430062,p.r.china [a] key laboratory for biological sciences of oil crops Ministry of Agriculture/Oil Crops Research Institute Chinese Academy of Agricultural Sciences Wuhan 430062 P.R. China

In eukaryotes,14-3-3 proteins constitute a family of ubiquitous regulatory *** play very important roles in many cell ***,their enconding genes and roles in plants remain to be *** this paper,four 14-3-3 genes from Brassica napus *** obtained by randomly sequencing a full-length cDNA library and named as Bn1433-1-4 *** phylogenetic comparison with Arabidopsis 14-3-3 family showed that Bn1433-1 and Bn1433-2 belonged to Episilon group while Bn1433-3 and Bn1433-4 belonged to non-Episilon *** transcript levels of four Bn1433 genes were analyzed in different organs,various stress conditions and some hormone treatments by real-time *** result showed that all the Bn1433s were expressed constitutively in roots,stems,leaves and immature seeds except that Bn1433-4 exhibited strong expression in immature seeds of 14 and 28 DAF（day after flowering）.By the analysis of real-time pCr,different expression patterns of Bn1433s were studied in various stress conditions and hormone *** was suggested that the functions of Bn1433s are diverse and they are involved in the regulation of various stresses and hormones treatments.

关键词： 14-3-3 expression pattem abiotic stress Brassica napus L.

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A Simple Method for Isolating Chloroplast DNA and Mitochondria DNA from the Same rapeseed Green Leaf Tissue

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Journal of Integrative agriculture 2012年第7期11卷 1212-1215页

作者： HU Zhi-yong zhan gao-miao wang han-zhong hua wei key laboratory for Biology and Genetic Improvement of oil crops Ministry of Agriculture/Oil Crops Research InstituteChinese Academyof Agriculture SciencesWuhan 430062P.R.China

In the study, we present a fast, simple and inexpensive protocol for isolating chloroplast and mitochondrial DNA from one rapeseed leaf tissue sample. The chloroplast and mitochondria were separated from the same green leaf tissue by differential centrifugations. The protocol is the first report that isolates plant chloroplast DNA （cpDNA） and mitochondrial DNA （mtDNA） from the same sample homogenate. The organelle DNA yield is 2-10 micrograms per gram of tissue; the DNA was fully restrictable and was successfully used for sequencing.

关键词： chloroplast DNA mitochondrial DNA organelle isolation rapeseed

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Important photosynthetic contribution of silique wall to seed yield related traits in Arabidopsis thaliana

Important photosynthetic contribution of silique wall to see...

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中国作物学会油料作物专业委员会第八次会员代表大会暨学术年会

作者： ZHU Xiao-yi zhanG Liang KUANG Chen GUO Yan huaNG Chun-qian DENG Lin-bin SUN Xing-chao zhan gao-miao HU Zhi-yong wang han-zhong hua wei key laboratory of Biology and Genetic Improvement of oil crops Ministry of AgricultureOil Crops Research Institute of the Chinese Academy of Agricultural Sciences

To date,contribution of pod photosynthesis to seed yield and related components still remains largely unexplored,and photosynthetic activity of the silique(pod) in Arabidopsis thaliana is *** this study,an Arabidopsis glk1/glk2 mutant defective in both leaf and silique photosynthesis was used to create tissue-specific functional complementation *** lines were used to analyze the contribution of silique wall photosynthesis to seed yield and related traits,and to permit the comparison of this contribution with that of leaf *** results showed that,together with leaf,photosynthetic assimilation of silique wall greatly contributed to total seed yield per *** for individual components of yield traits,leaf photosynthesis alone contributed to seed number per silique and silique length,while silique wall photosynthesis alone contributed to thousand-seed *** addition,enhancing photosynthetic capacity of silique wall by overexpressing photosynthesis-related rCA gene in this tissue resulted in significantly increased seed weight and oil content in the wild-type *** results revealed that silique wall photosynthesis played an important role in seed related *** finding may have significant implications for improving seed yield and oil production of oilseed crops and other species with pod-like organs.

关键词： photosynthesis silique wall seed yield traits Arabidopsis thaliana

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高油酸花生新品种豫花37号选育及遗传分析

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中国油料作物学报 2021年第4期43卷 608-616页

作者：秦利刘华张新友杜培代小冬孙子淇齐飞艳董文召谷建中黄冰艳韩锁义张忠信徐静高伟河南省农业科学院河南省作物分子育种研究院/农业农村部黄淮海油料作物重点实验室/河南省油料作物遗传改良重点实验室/花生遗传改良国家地方联合工程实验室河南郑州450002 河南生物育种中心有限公司河南郑州450002 河南省农业科学院经济作物研究所河南郑州450002 开封市农林科学研究院河南开封475000

豫花37号是以高产、广适性品种海花1号为母本,以高油酸小果开选016为父本,通过有性杂交和系谱法选择,在分离世代综合运用近红外品质检测、异地生态鉴定、分子标记辅助选择等育种方法选育而成的珍珠豆优质高油酸花生新品种。2012年和201... 详细信息

豫花37号是以高产、广适性品种海花1号为母本,以高油酸小果开选016为父本,通过有性杂交和系谱法选择,在分离世代综合运用近红外品质检测、异地生态鉴定、分子标记辅助选择等育种方法选育而成的珍珠豆优质高油酸花生新品种。2012年和2013年参加河南省珍珠豆型优质花生区试,两年9个试验点荚果平均单产达到4583.55 kg/hm^(2),比对照远杂9102(普通油酸品种)增产2.68%;2014年参加河南省珍珠豆花生生产试验,6个试验点荚果平均单产5084.4 kg/hm^(2),比对照远杂9102增产10.84%;于2015年通过河南省品种审定委员会审定,夏直播生育期116 d左右。本研究进一步对豫花37的遗传背景和控制油酸含量的两对主效基因的突变类型进行了分析,对后续高油酸品种选育具有一定借鉴意义。

关键词：高油酸珍珠豆花生

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