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Influence of dietary conjugated linoleic acid on growth, fatty acid composition and hepatic lipogenesis in large yellow croaker (Pseudosciaena crocea R.)

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Journal of zhejiang university-Science B(Biomedicine & Biotechnology) 2008年第9期9卷 691-700页

作者： Zhan-yu ZHAO1,2, Tian-xing WU1, Hong-gang TANG1, Ji-ze ZHANG3 (1department of Chemistry, zhejiang university, hangzhou 310027, china) (2Key Laboratory for Molecular Design and Nutrition Engineering, Ningbo Institute of Technology, zhejiang university, Ningbo 315100, china) (3School of Animal Sciences, zhejiang university, hangzhou 310027, china) department of Chemistry Zhejiang University Hangzhou 310027 China. zhanyuzhao@***

We examined the effects of conjugated linoleic acid (CLA) on growth, fatty acid composition and enzyme activity of fatty acid oxidation in the liver of large yellow croaker. We divided 1600 fish (average initial weight 150 g) into 4 groups and reared them in 8 cages. Four dietary treatments were formulated to contain 0%, 1%, 2% and 4% (w/w) CLA, respectively. The fish were fed for 10 weeks ad libitum twice daily. We found that the dietary CLA had no effect on growth, biometric parameters and whole body proximate (P>0.05), but showed some significant effects on the fatty acid composition in both muscle and the liver. The activities of lipogenic enzymes were slightly depressed in fish fed with increasing levels of CLA when compared with control (P>0.05). Dietary CLA supplementation had no effects on liver lipid content, but significantly increased the contents of saturated fatty acids (SFA) and polyunsaturated fatty acids (PUFA) (P<0.05) and decreased monounsaturated fatty acid (MUFA) content in both muscle and the liver. Dietary CLA inclusion resulted in significant increases of the biologically active cis-9, trans-11 and trans-10, cis-12 isomers in both tissues (P<0.05). The total accumulation of CLA was higher in the liver (3.83%, w/w) than in muscle (3.77%, w/w) when fed with 4% (w/w) CLA. This study demonstrates that large yellow croakers are capable of absorbing and depositing CLA and long-chain n-3 PUFA in the liver and muscle, showing that this species fed with CLA could be an important human food source for these healthful fatty acids.

关键词： Conjugated linoleic acid (CLA) Fatty acids Lipid Lipogenic enzymes Large yellow croaker

The　Optical　Implementation　of　High　Efficiency　Packed　Redundant　Binary　Number　Addition　Based　on　Boolean　Polarization－encoded　Logic　Algebra

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Chinese Journal of Lasers 1996年第3期B5卷 268-275页

作者： WANG Bin yu Feihong LIU Xu GU Peifu TANG Jinfa(State Key Laboratory of Modern Optical Instrument, Optical Engineering department,zhejiang university, hangzhou 310027, china) zhejiang Univ Hangzhou China

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关键词： packed redundant binary number boolean polarization-encoded logic algebra optical computing

Cloning of UGT1A9 cDNA from liver tissues and its expression in CHL cells

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World Journal of Gastroenterology 2001年第6期7卷 841-845页

作者： Xin Li~(1,2) Ying-Nian yu~1 Ge-Jian Zhu~1 yu-Li Qian~1 1 department of Pathophysiology,School of Medicine,zhejiang university,hangzhou,china2 College of Pharmcy,zhejiang university,hangzhou,china department of Pathophysiology School of Medicine Zhejiang University Hangzhou China.

AIM: To clone the cDNA of UGT1A9 from a Chinese human liver and establish the Chinese hamster lung (CHL) cell line expressing human UGT1A9. METHODS: cDNA of UGT1 A9 was transcripted from mRNA by reverse transcriptase-ploymerase chain reaction, and was cloned into the pGEM-T vector which was amplified in the host bacteric *** DH5(alpha). The inserted fragment, verified by DNA sequencing, was subcloned into the Hind III /Not I site of a mammalian expression vector pREP9 to construct the plasmid termed pREP9-UGT1A9. CHL cells were transfected with the resultant recombinants, pREP9-UGT1A9, and selected by G418 (400 mg x L(-1)) for one month. The surviving clone (CHL-UGT1A9) was harvested as a pool and sub-cultured in medium containing G418 to obtain samples forUGT1A9 assays. The enzyme activity of CHL-UGT1A9 towards propranolol in S9 protein of the cell was determined by HPLC. RESULTS: The sequence of the cDNA segment cloned, which was 1666 bp in length, was identical to that released by Gene Bank (GenBank accession number: AF056188) in coding region. The recombinant constructed, pREP9-UGT1A9, contains the entire coding region, along with 18 bp of the 5' and 55 bp of the 3' untranslated region of theUGT1A9 cDNA, respectively. The cell lines established expressed the protein of UGT1A9, and the enzyme activity towards propranolol in S9 protein was found to be 101+/- 24 pmol x min(-1) x mg(-1) protein (n=3), but was not detectable in parental CHL cells. CONCLUSION: The cDNA of UGT1A9 was successfully cloned from a Chinese human liver and transfected into CHL cells. The CHL-UGT1 A9 cell lines established efficiently expressed the protein ofUGT1A9 for the further enzyme study of drug glucuronidation.

关键词： Cloning, Molecular Gene Expression Animals Cell Line Cricetinae Cricetulus DNA, Complementary Glucuronosyltransferase Humans Liver Molecular Sequence Data Research Support, Non-U.S. Gov't

The Influence of Nano-AI2O3 Additive on the Adhesion between Epoxy Resin and Steel Substrate

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材料热处理学报 2004年第5期 1263-1266页

作者： zhai Lan-lan, LING Guo-ping department of Metallic Materials, zhejiang university, hangzhou, china

The influence of nano-A!2O3 additive on the adhesion between epoxy resin and steel substrate has been investigated. The results of tensile testing indicated that the adhesion strength was increased dramatically by addition of Al2Os nanoparticles in epoxy resin compared with that of the unmodified resin. The highest adhesion strength was obtained with 1 wt% nano-A!2O3 added in epoxy adhesive, more than two times higher than that of the unmodified. Scanning electronic microscope (SEM) revealed that a boundary layer exists between epoxy and steel substrate, energy spectrum analysis indicates there is enrichment of the nano-A!2O3 particle. Those results confirmed that the nano-A12O3 additive was closely related to the change of interface morphology and the improvement of adhesion strength. The reason for adhesion improvement was also be discussed.

关键词： nanoparticle A12O3 adhesive strength epoxy adhesive steel

Cloning of cytochrome P-450 2C9 cDNA from human liver and its expression in CHL cells

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World Journal of Gastroenterology 2002年第2期8卷 318-322页

作者： Ge-Jian Zhu Ying-Nian yu,department of Pathophysiology and Laboratory of Medical Molecular Biology,zhejiang university School of Medicine,hangzhou 310031,zhejiang Province,china Xin Li,department of pharmaceutical analysis & drug metabolism,College of Pharmacology Science,zhejiang university,hangzhou 310031,zhejiang Province,china yu-Li Qian, Present address:Center of laboratory,Women’s hospital,School of Medicine,zhejiang university,hangzhou 310031,zhejiang Province,china department of Pathophysiology and Laboratory of Medical Molecular Biology Zhejiang University School of Medicine Hangzhou 310031 Zhejiang Province China.

AIM: Using bacterial, yeast, or mammalian cell expressing a human drug metabolism enzyme would seem good way to study drug metabolism-related problems. Human cytochrome P-450 2C9(CYP2C9) is a polymorphic enzyme responsible for the metabolism of a large number of clinically important drugs. It ranks among the most important drug metabolizing enzymes in humans. In order to provide a sufficient amount of the enzyme for drug metabolic research, the CYP2C9 cDNA was cloned and expressed stably in CHL cells. METHODS: After extraction of total RNA from human liver tissue, the human CYP2C9 cDNA was amplified with reverse transcription-polymerase chain reaction (RT-PCR), and cloned into cloning vector pGEM-T. The cDNA fragment was identified by DNA sequencing and subcloned into a mammalian expression vector pREP9. A transgenic cell line was established by transfecting the recombinant vector of pREP9-CYP2C9 into CHL cells. The enzyme activity of CYP2C9 catalyzing oxidation of tolbutamide to hydroxy tolbutamide in S9 fraction of the cell was determined by high performance liquid chromatography(HPLC). RESULTS: The amino acid sequence predicted from the cDNA segment was identical to that of CYP2C9*1, the wild type CYP2C9. However, there were two base differences, i.e. 21T>C, 1146C>T, but the encoding amino acid sequence was the same, L7, P382. The S9 fraction of the established cell line metabolizes tolbutamide to hydroxy tolbutamide; tolbutamide hydroxylase activity was found to be 0.465 +/- 0.109 ***(-1).g(-1) S9 protein or 8.62 +/- ***(-1).mol(-1) CYP, but was undetectable in parental CHL cell. CONCLUSION: The cDNA of human CYP2C9 was successfully cloned and a cell line of CHL- CYP2C9, efficiently expressing the protein of CYP2C9, was established.

关键词： Cloning, Molecular Animals Aryl Hydrocarbon Hydroxylases Cell Fractionation Cell Line china Gene Expression Humans Hypoglycemic Agents Liver Protein Isoforms Recombinant Proteins Research Support, Non-U.S. Gov't Tolbutamide

Achieving laparoscopic anatomic resection for hepatocellular carcinoma by fluorescence guided positive staining of hepatic cone unit

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Hepatobiliary & Pancreatic Diseases International 2024年第4期23卷 406-411页

作者： Jun-Fang Deng Chuan-Hui Peng yu Zhang Xiao-Feng Xu Rong-Liang Tong Zheng-Long zhai Di-yu Chen Cheng Zhang Li-Ming Wu Jian Wu Division of Hepatobiliary Pancreatic Surgery the First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China

Laparoscopic anatomical liver resection has been increasingly reported worldwide,but how to demarcate the resection planes is technically challenging[1].Positive staining technique with intraoperative indocyanine green(ICG)-fluorescence imaging has been introduced as an effective tool for real-time intraoperative guidance[2].Liver anatomy is ***,liver is composed of 9 segments,each is composed of two to three subsegments,and each subsegment contains several hepatic cone units.A hepatic cone unit is dominated by a tertiary or quaternary hepatic pedicles[3].

关键词： hepatic resection anatomy

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Expression,Characterization and Immunogenicity of a Major Outer Membrane Protein from Vibrio alginolyticus

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Acta Biochimica et Biophysica Sinica 2007年第3期39卷 194-200页

作者： Ronghua QIAN~(1,2) Wuying CHU~3 Zhijuan MAO~(1,4) Chongwen ZHANG~(1,2) Yongwei WEI~2 Lian yu~2* 1 department of Biomedical Engineering,zhejiang university,hangzhou 310027,china 2 Institute of Preventive Veterinary Medicine,zhejiang university,hangzhou 310029,china 3 Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,china 4 Ningbo Oceanography and Fishery Institute,Ningbo 315012,china department of Biomedical Engineering Zhejiang UniversityHangzhou 310027 China Institute of Preventive Veterinary Medicine Zhejiang UniversityHangzhou 310029 China Institute of Subtropical Agriculture Chinese Academy of SciencesChangsha 410125 China Ningbo Oceanography and Fishery InstituteNingbo 315012 China

Vibrio alginolyticus is one of the Vibrio pathogens common to humans and marine *** infection and induction of the host immune response,outer membrane proteins of bacteria play animportant *** this study,an outer membrane protein gene(ompW)was cloned from *** andexpressed in Escherichia *** 645 bp open reading frame(ORF)encodes a protein of 214 amino acidresidues with a predicted molecular weight of 23.3 *** amino acid sequence showed a high identitywith that of Photobacterium damselae(96.2%)and Vibrio parahaemolyticus(94.4%).The alignment analy-sis indicated that OmpW was highly *** dodecyl sulfate-polyacrylamide gel electrophoresisshowed that the gene was over-expressed in *** BL21(DE3).Western blot analysis revealed that theexpressed protein had *** recombinant protein was purified by affinity chromatographyon Ni-NTA Superflow *** yellow croaker vaccinated with the purified OmpW showed significantlyincreased antibody to OmpW,which could resist the infection by ***.A specific antibody wasdetected by enzyme-linked immunosorbent *** study suggested that the conserved OmpW could bean effective vaccine candidate against infection by ***.

关键词： Vibrio alginolyticus ompW expression characterization immunogenicity vaccine candidate

Effects of repeated applications of fungicide carbendazim on its persistence and microbial community in soil

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Journal of Environmental Sciences 2009年第2期21卷 179-185页

作者： yu yunlong,CHU Xiaoqiang,PANG Guohui,XIANG yueqin,FANG Hua department of Plant Protection,College of Agriculture and Biotechnology,zhejiang university,hangzhou 310029,china. department of Plant Protection College of Agriculture and Biotechnology Zhejiang University Hangzhou 310029 China

Carbendazim, a systemic benzimidazole fungicide, is applied repeatedly to control plant diseases including soilborne diseases, over a growing season. Studies were carried out under laboratory conditions to assess the effects of repeated carbendazim applications on its persistence and microbial community in soil. The results indicate that dissipation of carbendazim in soil was accelerated with its application frequency. The degradation rate constant of carbendazim was increased significantly from 0.074 d-1 to 0.79 d-1. The corresponding half-life was shorten markedly from 9.3 d to 0.9 d after four repeated applications. No significant inhibitory effect of carbendazim on soil microbial utilization of the carbon sources was observed after first treatment, but a slight increase in average well color development （AWCD） was shown after second, third, and fourth applications. It suggested that soil microorganisms become adapted to carbendazim after repeated application. Simpson and Shannon indexes of soil microbial community from carbendazim treated soil were also similar to those from the control soil, indicating that the richness and dominant character of soil microorganisms remain unchangeable after repeated application. However, after first, second, and third addition of carbendazim, McIntosh indexes on day 21 were significantly higher compared with the control, suggesting that balance of soil microorganisms was altered due to the enrichment of the specific carbendazim-adapting strains in soil

关键词： carbendazim degradation soil microorganism repeated application

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Trogocytosis of CAR molecule regulates CAR-T cell dysfunction and tumor antigen escape

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Signal Transduction and Targeted Therapy 2024年第1期9卷 326-337页

作者： You zhai Yicong Du Guanzhang Li Mingchen yu Huimin Hu Changqing Pan Di Wang Zhongfang Shi Xu Yan Xuesong Li Tao Jiang Wei Zhang department of Molecular Neuropathology Beijing Neurosurgical InstituteCapital Medical UniversityBeijingPR China department of Neurosurgery Beijing Tiantan HospitalCapital Medical UniversityBeijingPR China department of Urology Peking University First HospitalInstitute of UrologyPeking UniversityNational Urological Cancer CenterBeijingPR China department of Pathophysiology Beijing Neurosurgical InstituteCapital Medical UniversityBeijingPR China china National Clinical Research Center for Neurological Diseases BeijingPR China Center of Brain Tumor Beijing Institute for Brain DisordersBeijingPR China Research Unit of Accurate Diagnosis Treatmentand Translational Medicine of Brain TumorsChinese Academy of Medical SciencesBeijingPR China Chinese Glioma Genome Atlas Network(CGGA)and Asian Glioma Genome Atlas Network(AGGA) BeijingPR China

Chimeric antigen receptor(CAR)T-cell therapy has demonstrated clinical response in treating both hematologic malignancies and solid *** instances of rapid tumor remissions have been observed in animal models and clinical trials,tumor relapses occur with multiple therapeutic resistance ***,while the mechanisms underlying the long-term therapeutic resistance are well-known,short-term adaptation remains less ***,more views shed light on short-term adaptation and hold that it provides an opportunity window for long-term *** this study,we explore a previously unreported mechanism in which tumor cells employ trogocytosis to acquire CAR molecules from CAR-T cells,a reversal of previously documented *** mechanism results in the depletion of CAR molecules and subsequent CAR-T cell dysfunction,also leading to short-term antigen loss and antigen *** type of intercellular communication is independent of CAR downstream signaling,CAR-T cell condition,target antigen,and tumor cell ***,it is mainly dependent on antigen density and CAR sensitivity,and is associated with tumor cell cholesterol *** mitigation of this trogocytosis-induced CAR molecule transfer can be achieved by adaptively administering CAR-T cells with antigen density-individualized CAR ***,our study reveals a dynamic process of CAR molecule transfer and refining the framework of clinical CAR-T therapy for solid tumors.

关键词： Together instance depletion