检索结果-南通市图书馆

TPOL triggers apoptosis with mitochondrial injury through activating a ROS-dependent p53/p21/p27/Rb/Bax/Cyto C/caspase-mediated signaling

在线全文

学校读者我要写书评

暂无评论

中国病理生理杂志 2024年第8期40卷 1488-1496页

作者： cheng Zongwei zeng Boning XING Feiyue Department of Immunobiology Institute of Tissue Transplantation and ImmunologyJinan UniversityGuangzhou 510632China

AIM:To explore the influence of ethyl(2,4,6-trimethylbenzoyl)phenylphosphinate(TPOL)on cell apoptosis and its potential ***:HEK293T cells sensitive to TPOL were treated with different concentrations of TPOL with or without exposure to light radiation,before treatment with various inhibitors,N-acetyl-Lcysteine(NAC),pifithrin-αand *** viability was measured by CCK-8 *** V/propidium iodide staining was used to count the number of apoptotic ***-DA staining was used to detect reactive oxygen species(ROS)levels,and JC-1 staining was used to assess mitochondrial membrane potential by flow *** expression of apoptosis-related proteins and cell cycle-regulated molecules was measured by Western ***:TPOL enhanced the apoptosis of HEK293T cells in a dose-dependent manner(P<0.05),with a decrease in Bcl-2 and increases in Bax and cytochrome C(Cyto C),followed by up-regulation of activated caspase-9 and caspase-3,and the cleavage of PARP(P<0.05).The TPOL-enhanced cleavage of caspase-3 and PARP was rescued by Z-DVED-FMK(P<0.01).TPOL also led to a rapid increase in ROS,a reduction in mitochondrial membrane potential,and the release of Cyto C(P<0.01),all of which could be reversed by the ROS scavenger ***,the TPOL-caused alterations in p21,p27,Rb,and CDK2 were also recovered by the p53 inhibitor pifithrin-α(P<0.05).The TPOL-induced changes in Bax,Bcl-2,cleaved caspase-9,activated caspase-3,and cleaved PARP were subsequently rescued by pretreatment with pifithrin-α(P<0.05).CONCLUSION:TPOL can induce cellular apoptosis with ROS-mediated mitochondrial membrane damage through the activation of a ROS-dependent p53/p21/p27/Rb/Bax/Cyto C/caspase-mediated signal axis.

关键词： ethyl(2,4,6-trimethylbenzoyl)phenylphosphinate reactive oxygen species mitochondria apoptosis

Investigation on photonic crystal nanobeam cavity based on mixed diamond–circular holes

在线全文

学校读者我要写书评

暂无评论

Nanotechnology and Precision Engineering 2024年第1期7卷 63-70页

作者： Jingtong Bin Kerui Feng Shang Ma Ke Liu Yong cheng Jing Chen Qifa Liu College of Telecommunication and Information Engineering Nanjing University of Posts and TelecommunicationsNanjing 210003China College of Electronic and Optical Engineering and College of Flexible Electronics(Future Technology) Nanjing University of Posts and TelecommunicationsNanjing 210003China State Key Laboratory of Advanced Optical Communication Systems and Networks Shanghai Jiao Tong UniversityShanghai 200240China

A photonic crystal nanobeam cavity(M-PCNC)with a structure incorporating a mixture of diamond-shaped and circular air holes is *** performance of the cavity is simulated and studied *** theﬁnite-difference time-domain method,the parameters of the M-PCNC,including cavity thickness and width,lattice constant,and radii and numbers of holes,are optimized,with the quality factor Q and mode volume Vm as performance *** modulation of the lattice constant and hole radius enable the proposed M-PCNC to realize outstanding *** optimized cavity possesses a high quality factor Q 1.45105 and an ultra-small mode=×volume Vm 0.01(λ/n)[zeng et al.,Opt Lett 2023:48;3981–3984]in the telecommunications wavelength *** can be progres-=sively squeezed in both the propagation direction and the perpendicular in-plane direction by a series of interlocked anti-slots and slots in the diamond-shaped hole ***,the energy can be conﬁned within a small mode volume to achieve an ultra-high Q/Vm ratio.

关键词： Photonic crystal nanobeam cavity Mixed diamond–circular holes Slots and anti-slots FDTD simulation Quality factor Mode volume

Targeting HMGCS1 restores chemotherapy sensitivity in acute myeloid leukemia

在线全文

学校读者我要写书评

暂无评论

Blood Science 2024年第3期6卷 11-22页

作者： cheng Zhou Jue Li Xiaofan Sun Liang Zhao Huien Zhan Hui Liang Peng Fang Tuo Zhang Qiongzhi He Juan Du Hui zeng Department of Hematology The First Affiliated Hospital of Jinan UniversityGuangzhouGuangdong 510630China Department of Hematology Xiangya HospitalCentral South UniversityChangshaHunan 410008China Genomic Core Weill Cornell Medical CollegeNew YorkNY 10021USA Geneplus-Beijing Institute Beijing 102206China

Acute myeloid leukemia(AML)is a common hematological malignancy with overall poor *** novel targets is urgent and necessary to improve the clinical outcome of relapsed and refractory(RR)AML *** clinical specimens,animal models and cell-level studies,we explored the specific mechanism of 3-hydroxy-3-methylglutaryl coenzyme A synthase 1(HMGCS1)in AML and the mechanism of targeting HMGCS1 to attenuate cell proliferation,increase chemotherapy sensitivity and improve the occurrence and development of ***,we reveal that HMGCS1 is overexpressed in RR patients and negatively related to overall survival(OS).Knocking out HMGCS1 in AML cells attenuated cell proliferation and increased chemotherapy sensitivity,while stable overexpression of HMGCS1 had the opposite ***,we identified that knockout of HMGCS1 suppressed mitogen-activated protein kinase(MAPK)pathway activity,while overexpression of HMGCS1 could remarkably enhance the pathway.U0126,a MEK1 inhibitor,offset the effects of HMGCS1 overexpression,indicating that HMGCS1 promotes RR AML through the MAPK ***,we verified that hymeglusin,a specific inhibitor of HMGCS1,decreases cell growth both in AML cell lines and primary bone marrow cells of AML ***,combination of hymeglusin and the common chemotherapeutic drug cytarabine and adriamycin(ADR)had synergistic toxic effects on AML *** study demonstrates the important role of HMGCS1 in AML,and targeting this protein is promising for the treatment of RR AML.

关键词： Chemotherapy sensitivity HMGCS1 MAPK pathway Relapsed and refractory AML Targeted therapy

The chromosome-scale assembly of the Notopterygium incisum genome provides insight into the structural diversity of coumarins

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2024年第8期14卷 3760-3773页

作者： Qien Li Yiqun Dai Xin-cheng Huang Lanlan Sun Kaixuan Wang Xiao Guo Dingqiao Xu Digao Wan Latai An Zixuan Wang Huanying Tang Qi Qi Huihui zeng Minjian Qin Jia-Yu Xue Yucheng Zhao Tibetan Medicine Research Center of Qinghai University Tibetan Medical CollegeQinghai UniversityXining 810016China School of Pharmacy Bengbu Medical UniversityBengbu 233030China Department of Resources Science of Traditional Chinese Medicines School of Traditional Chinese PharmacyChina Pharmaceutical UniversityNanjing 210009China College of Horticulture Bioinformatics CenterAcademy for Advanced Interdisciplinary StudiesNanjing Agricultural UniversityNanjing 210095China Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility Shaanxi University of Chinese MedicineXi’an 712046China

Coumarins,derived from the phenylpropanoid pathway,represent one of the primary metabolites found in *** alignment of the tetrahydropyran(THP)and tetrahydrofuran(THF)rings with the lactone structure results in the formation of at least four types of complex ***,the mechanisms underlying the structural diversity of coumarin remain poorly ***,we report the chromosome-level genome assembly of Notopterygium incisum,spanning 1.64 Gb,with a contig N50 value of 22.7 Mb and 60,021 annotated protein-coding ***,we identified the key enzymes responsible for shaping the structural diversity of coumarins,including two p-coumaroyl CoA 2′-hydroxylases crucial for simple coumarins basic skeleton architecture,two UbiA prenyltransferases responsible for angular or linear coumarins biosynthesis,and five CYP736 cyclases involved in THP and THF ring ***,two bifunctional enzymes capable of catalyzing both demethylsuberosin and osthenol were identified for the first *** analysis implies that tandem and ectopic duplications of the CYP736 subfamily,specifically arising in the Apiaceae,contributed to the structural diversity of coumarins in ***,this study proposes a parallel evolution scenario for the complex coumarin biosynthetic pathway among different angiosperms and provides essential synthetic biology elements for the heterologous industrial production of coumarins.

关键词： Notopterygium incisum Parallel evolution Biosynthesis Pyranocoumarins Furanocoumarins

基于离散采样模型的三自由度双旋翼直升机的控制器设计

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

系统科学与数学 2024年第9期44卷 2588-2602页

作者：迟楠曾诚贵州理工学院理学院贵阳550003

三自由度纵列式双旋翼直升机模型是动力学系统研究中的一类重要实验对象,也是研究直升机飞行控制技术的平台.文章首先针对非线性三自由度纵列式直升机模型的系统描述,在其标准形描述和一般采样保持条件下,导出了比经典的Euler模型更精... 详细信息

三自由度纵列式双旋翼直升机模型是动力学系统研究中的一类重要实验对象,也是研究直升机飞行控制技术的平台.文章首先针对非线性三自由度纵列式直升机模型的系统描述,在其标准形描述和一般采样保持条件下,导出了比经典的Euler模型更精确的非线性近似离散时间模型,并进一步计算可得出,它们的近似离散模型的局部截断误差和全局截断误差分别为T5和T2,其中T为采样周期.其次,文章对非线性三自由度双旋翼直升机进行了系统辨识及其相应的简单讨论.最后,在导出的近似直升机离散采样模型的基础上,对非线性三自由度双旋翼直升机,文章给出了连续时间输入控制设计和李雅普诺夫再设计两种方法来开展对比研究,并通过实例表明李雅普诺夫再设计可增强系统的控制性能.

关键词：三自由度双旋翼直升机离散采样分析系统辨识采样控制器设计

Postoperative epidural hematoma as a rare complication after intracranial tumor resection: a case series report and causes analysis

在线全文

学校读者我要写书评

暂无评论

中华神经外科杂志(英文) 2024年第2期10卷 83-91页

作者： zeng Minghui Li Zhijin Xia Chunsheng cheng Xufeng Wang Yehan Wang Fei Department of Neurosurgery The First Affiliated Hospital of USTC (Anhui Provincial Hospital) Division of Life Science and Medicine University of Science and Technology of China Hefei Anhui China

Background To review the treatment and the causes of postoperative epidural hematoma (PEDH) after intracranial tumor *** A retrospective case study was conducted to examine a series of patients who developed PEDH as a complication following intracranial tumor resection between January 2016 and June 2021. The study collected data from hospital charts, including clinical status at admission, imaging results, histopathologic findings, surgical management, complications, and outcomes. Causes of PEDH were evaluated through a review of operative notes and discussions with the surgical *** Twenty-five patients (10 males, 15 females; median age 42 years, range 11-61 years; median medical history 27 months, range 1-96 months) were enrolled in the study. Regarding tumor location, 16 cases exhibited supratentorial brain tumors, 4 cases had infratentorial brain tumors, 2 cases of tumors occurred in the petroclival region, 2 cases in the peritorcular region, and 1 case in the pineal region. Four of these cases were complicated with supratentorial hydrocephalus. The 25 cases in this study were classified into four types based on location. Type 1 refers to EDHs that occur at the adjacent site of the operative field without involvement of the surgical area. Type 2 includes hematomas that occur at the adjacent site of the surgical area and the surgical area. Type 3 includes EDHs that occur in distant areas, and type 4 involves EDHs in the surgical field. The numbers of cases of types 1, 2, 3, and 4 PEDHs were 16, 2, 3, and 4 cases, respectively. Most PEDHs were associated with reduced ICP after craniotomy due to intracranial tumor resection and substantial loss of CSF. All patients achieved satisfactory outcomes after hematoma *** The decrease in ICP resulting from intracranial tumor resection and CSF loss might lead to PEDHs. By employing optimized surgical techniques and meticulous patient management to prevent rapid decreases in ICP and dur

关键词： Epidural hematoma Postoperative complication Craniotomy Brain tumor

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

第二军医大学学报 2015年第10期36卷 1138-1142页

作者：曾程程魏锐利牟旆王秋红第二军医大学长征医院眼科上海200433

目的探讨可溶性CD40L（sCD40L）对甲状腺相关性眼病（TAO）患者眼眶成纤维细胞（OFs）增殖和3种透明质酸合成酶（HAS）mRNA表达水平的影响,初步探讨sCD40L在TAO发病中的作用。方法取5例TAO患者和3例正常对照标本原代培养OFs,采用MTS... 详细信息

目的探讨可溶性CD40L（sCD40L）对甲状腺相关性眼病（TAO）患者眼眶成纤维细胞（OFs）增殖和3种透明质酸合成酶（HAS）mRNA表达水平的影响,初步探讨sCD40L在TAO发病中的作用。方法取5例TAO患者和3例正常对照标本原代培养OFs,采用MTS比色法检测终质量浓度6.25、12.5、25、50、100、200ng/mL的sCD40L作用48h后对不同来源OFs增殖的影响;实时荧光定量PCR技术检测终质量浓度12.5、25、50、100ng/mL及作用3、6、12、24h后的sCD40L对不同来源OFs的3种HAS基因（HAS1~3）表达水平的影响。结果 25ng/mL及以上浓度的sCD40L作用48h后对TAO患者OFs有促增殖作用（P〈0.05）;而50ng/mL及以上浓度的sCD40L作用48h后才能对正常对照OFs有促增殖作用（P〈0.05）,且作用较弱。TAO患者OFs中HAS3mRNA的合成在sCD40L的刺激下增加,并且呈现出浓度和时间依赖的趋势。结论 sCD40L能够促进TAO患者OFs的生长以及HAS3基因的表达,提示sCD40L在TAO的病理过程中起一定作用。

关键词：可溶性CD40配体 Graves眼病眼眶成纤维细胞透明质酸合成酶

Unveiling a novel metal-to-metal transition in LuH_(2):Critically challenging superconductivity claims in lutetium hydrides

在线全文

学校读者我要写书评

暂无评论

Matter and Radiation at Extremes 2024年第3期9卷 65-73页

作者： Dong Wang Ningning Wang Caoshun Zhang Chunsheng Xia Weicheng Guo Xia Yin Kejun Bu Takeshi Nakagawa Jianbo Zhang Federico Gorelli Philip Dalladay-Simpson Thomas Meier Xujie Lü Liling Sun Jinguang cheng Qiaoshi zeng Yang Ding Ho-kwang Mao Center for High-Pressure Science and Technology Advanced Research Beijing 100094China Beijing National Laboratory for Condensed Matter Physics and Institute of Physics Chinese Academy of SciencesBeijing 100190China Shanghai Key Laboratory of Material Frontiers Research in Extreme Environments(MFree) Shanghai Advanced Research in Physical Sciences(SHARPS)Shanghai 201203China

Following the recent report by Dasenbrock-Gammon et al.[Nature 615,244–250(2023)]of near-ambient superconductivity in nitrogendoped lutetium trihydride(LuH_(3-δ)N_(ε)),significant debate has emerged surrounding the composition and interpretation of the observed sharp resistance ***,we meticulously revisit these claims through comprehensive characterization and *** definitively identify the reported material as lutetium dihydride(LuH_(2)),resolving the ambiguity surrounding its *** similar conditions(270–295 K and 1–2 GPa),we replicate the reported sharp decrease in electrical resistance with a 30%success rate,aligning with the observations by Dasenbrock-Gammon et ***,our extensive investigations reveal this phenomenon to be a novel pressure-induced metal-to-metal transition intrinsic to LuH_(2),distinct from ***,nitrogen doping exerts minimal impact on this *** work not only elucidates the fundamental properties of LuH_(2)andLuH_(3),but also critically challenges the notion of superconductivity in these lutetium hydride *** findings pave the way for future research on lutetium hydride systems,while emphasizing the crucial importance of rigorous verification in claims of ambient-temperature superconductivity.

关键词： resistance hydride superconductivity

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

红细胞分布宽度与甲状腺相关性眼病相关

第二军医大学学报 2015年第6期36卷 680-684页

作者：曾程程魏锐利牟旆王秋红第二军医大学长征医院眼科上海200003

目的探讨红细胞分布宽度(red cell distribution width,RDW)与甲状腺相关性眼病(thyroid-associated ophthalmopathy,TAO)的关系。方法收集52例格雷夫斯病(Graves’disease,GD)患者[男18例、女34例,39例有格雷夫斯眼病(Graves’ophthalmopathy,GO)表现]和22例健康对照者(男8例、女14例)资料。所有患者均检测RDW和甲状腺功能,GD组患者检查眼球突出度和临床活动性评分(CAS),比较组间差异。结果 GD患者的甲状腺功能各项指标与健康对照者相比差异有统计学意义(P<0.05);在GD患者中,有GO表现者和无GO表现者之间甲状腺功能相关指标差异无统计学意义,但有GO表现者的眼球突出度值高于无GO表现者(P<0.05)。GD患者的RDW高于健康对照者(P<0.05);在GD患者中,RDW与CAS评分呈现平行增加,且有GO表现者的RDW高于无GO表现者(P<0.05)。结论 RDW与GD患者和GO患者的病情相关,提示RDW可间接为评估病情的轻重提供依据。

关键词：甲状腺相关性眼病格雷夫斯病自身免疫性疾病红细胞分布宽度