检索结果-南通市图书馆

Protective efficacy of an H5/H7 trivalent inactivated vaccine(H5-Re13,H5-Re14, and H7-Re4 strains) in chickens, ducks, and geese against newly detected H5N1, H5N6, H5N8, and H7N9 viruses

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2022年第7期21卷 2086-2094页

作者： zeng xian-ying HE Xin-wen MENG Fei MA Qi WANG Yan BAO Hong-mei LIU Yan-jing DENG Guo-hua SHI Jian-zhong LI Yan-bing TIAN Guo-bin CHEN Hua-lan State Key Laboratory of Veterinary Biotechnology National Poultry Laboratory Animal Resource CenterHarbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China

Some H5 viruses isolated in poultry or wild birds between 2020 and 2021 were found to be antigenically different from the vaccine strains(H5-Re11 and H5-Re12) used in China. In this study, we generated three new recombinant vaccine seed viruses by using reverse genetics and used them for vaccine production. The vaccine strain H5-Re13 contains the hemagglutinin(HA) and neuraminidase(NA) genes of an H5 N6 virus that bears the clade 2.***.4.4 h HA gene, H5-Re14 contains the HA and NA genes of an H5 N8 virus that bears the clade 2.***.4.4 b HA gene, and H7-Re4 contains the HA and NA genes of H7 N9 virus detected in 2021. We evaluated the protective efficacy of the novel H5/H7 trivalent inactivated vaccine in chickens, ducks, and geese. The inactivated vaccine was immunogenic and induced substantial antibody responses in the birds tested. Three weeks after vaccination, chickens were challenged with five different viruses detected in 2020 and 2021: three viruses(an H5 N1 virus, an H5 N6 virus, and an H5 N8 virus) bearing the clade 2.***.4.4 b HA gene, an H5 N6 virus bearing the clade 2.***.4.4 h HA gene, and an H7 N9 virus. All of the control birds shed high titers of virus and died within 4 days post-challenge, whereas the vaccinated chickens were completely protected from these viruses. Similar protective efficacy against H5 viruses bearing the clade 2.***.4.4 h or 2.***.4.4 b HA gene was observed in ducks and geese. Our study indicates that the newly updated H5/H7 vaccine can provide solid protection against the H5 and H7 N9 viruses that are currently circulating in nature.

关键词： avian influenza H5/H7 trivalent vaccine H5-Re13 H5-Re14 H7-Re4 strains protective efficacy chickens ducks and geese

Development of a real-time RT-PCR method for the detection of newly emerged highly pathogenic H7N9 influenza viruses

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2017年第9期16卷 2055-2061页

作者： WANG Xiu-rong GU Lin-lin SHI Jian-zhong XU Hai-feng ZHANG ying zeng xian-ying DENG Guo-hua LI Cheng-jun CHEN Hua-lan State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China

In 2013, a human influenza outbreak caused by a novel H7N9 virus occurred in China. Recently, the H7N9 virus acquired multiple basic amino acids at its hemagglutinin（HA） cleavage site, leading to the emergence of a highly pathogenic virus. The development of an effective diagnostic method is imperative for the prevention and control of highly pathogenic H7N9 influenza. Here, we designed and synthesized three pairs of primers based on the nucleotide sequence at the HA cleavage site of the newly emerged highly pathogenic H7N9 influenza virus. One of the primer pairs and the corresponding probe displayed a high level of amplification efficiency on which a real-time RT-PCR method was established. Amplification using this method resulted in a fluorescent signal for only the highly pathogenic H7N9 virus, and not for any of the H1–H15 subtype reference strains, thus demonstrating high specificity. The method detected as low as 39.1 copies of HA-positive plasmid and exhibited similar sensitivity to the virus isolation method using embryonated chicken eggs. Importantly, the real-time RT-PCR method exhibited 100% consistency with the virus isolation method in the diagnosis of field samples. Collectively, our data demonstrate that this real-time RT-PCR assay is a rapid, sensitive and specific method, and the application will greatly aid the surveillance, prevention, and control of highly pathogenic H7N9 influenza viruses.

关键词： H7N9 highly pathogenic influenza virus real-time RT-PCR

Utility of Droplet Digital PCR Assay for Quantitative Detection of Norovirus in Shellfish, from Production to Consumption in Guangxi, China

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2018年第10期31卷 713-720页

作者： TAN Dong Mei LYU Su Ling LIU Wei zeng xian ying LAN Lan QU Cong ZHUGE Shi Yang ZHONG Yan Xu XIE Yi Hong LI Xiu Gui Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control Nanning 530028 Guangxi China

Objective Shellfish are recognized as important vehicles of norovirus-associated gastroenteritis. The present study aimed to monitor norovirus contamination in oysters along the farm-to-fork continuum in Guangxi, a major oyster production area in Southwestern China. Methods Oyster samples were collected monthly from farms, markets, and restaurants, from January to December 2016. Norovirus was detected and quantified by one-step reverse transcription-droplet digital polymerase chain reaction（RT-ddPCR）. Results A total of 480 oyster samples were collected and tested for norovirus genogroups I and II. Norovirus was detected in 20.7% of samples, with genogroup II predominating. No significant difference was observed in norovirus prevalence among different sampling sites. The norovirus levels varied widely, with a geometric mean of 19,300 copies/g in digestive glands. Both norovirus prevalence and viral loads showed obvious seasonality, with a strong winter bias. Conclusion This study provides a systematic analysis of norovirus contamination ‘from the farm to the fork＇ in Guangxi. RT-ddPCR can be a useful tool for detection and quantification of low amounts of norovirus in the presence of inhibitors found particularly in foodstuffs. This approach will contribute to the development of strategies for controlling and reducing the risk of human illness resulting from shellfish consumption.

关键词： Norovirus Droplet Digital PCR Shellfish Quantitative detection

在线全文

学校读者我要写书评

暂无评论

H7N9禽流感病毒进化变异研究最新进展

中国预防兽医学报 2021年第6期43卷 686-686页

作者： YIN XIN DENG GUO-HUA zeng xian-ying State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute CAAS Harbin People’s Republic of China College of Life and Health Sciences Chubu University Aichi Japan

H7N9亚型低致病性禽流感病毒2013年首次出现在我国南方家禽活禽交易市场,至2017年9月共引起5个波次的人流感疫情,导致1 560多人感染,致死率近40%。2017年初,国家禽流感参考实验室发现广东省的活禽市场分离到的部分病毒已突变为高致病性H... 详细信息

H7N9亚型低致病性禽流感病毒2013年首次出现在我国南方家禽活禽交易市场,至2017年9月共引起5个波次的人流感疫情,导致1 560多人感染,致死率近40%。2017年初,国家禽流感参考实验室发现广东省的活禽市场分离到的部分病毒已突变为高致病性H7N9病毒。进一步的研究表明该H7N9高致病性病毒对家禽和公共卫生具有更大威胁。

关键词：禽流感活禽市场致病性病毒参考实验室公共卫生活禽交易市场致死率

Protective efficacy of an H5/H7 trivalent inactivated vaccine produced from Re-11, Re-12, and H7-Re2 strains against challenge with different H5 and H7 viruses in chickens

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2020年第9期19卷 2294-2300页

作者： zeng xian-ying CHEN Xiao-han MA Shu-jie WU Jiao-jiao BAO Hong-mei PAN Shu-xin LIU Yan-jing DENG Guo-hua SHI Jian-zhong CHEN Pu-cheng JIANG Yong-ping LI Yan-bing HU Jing-lei LU Tong MAO Sheng-gang GUO Xing-fu LIU Jing-li TIAN Guo-bin CHEN Hua-lan State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China Harbin Weike Biotechnology Development Company Harbin 150069P.R.China

We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5N6) (DK/GZ/S4184/17) (a clade 2.***.4.4d virus), A/chicken/Liaoning/SD007/2017(H5N1) (CK/LN/SD007/17) (a clade 2.***.2.1d virus), and A/chicken/ Guangxi/SD098/2017(H7N9) (CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used H5/H7 bivalent inactivated vaccine against different H5 and H7N9 viruses was evaluated in chickens. We found that the H5/H7 bivalent vaccine provided solid protection against the H7N9 virus CK/GX/SD098/17, but only 50–60% protection against different H5 viruses. In contrast, the novel H5/H7 trivalent vaccine provided complete protection against the H5 and H7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control.

关键词： avian influenza H5/H7 trivalent vaccine Re-11 Re-12 and H7-Re2 strains protective efficacy

Microstructure and mechanical properties of Ti/Al/Ti clad plates prepared via powder-in-tube method

在线全文

学校读者我要写书评

暂无评论

Journal of Iron and Steel Research(International) 2024年第3期31卷 670-687页

作者： xian-lei Hu Qin-cheng Xie Yi Yuan ying Zhi State Key Laboratory of Rolling and Automation Northeastern UniversityShenyang 110819LiaoningChina Suzhou Dongbao Haixing Metal Material Technology Co. Ltd.Suzhou 215600JiangsuChina School of Material Science and Engineering Northeastern UniversityShenyang 110819LiaoningChina

The Ti/Al/Ti clad plates exhibit a broad spectrum of potential applications.However,the conventional techniques of hot-pressing composite and explosive composite are intricate and environmentally hazardous.A novel method was introduced for preparing clad plates,namely,the powder-in-tube method.This method involves a combination of cold rolling,annealing at a temperature of 550℃,and double rolling.The morphology of the intermetallic compound layer was analyzed through the utilization of interface stripping test,tensile test,and microscopic characterization.The interface morphology,interface bonding properties,tensile fracture structures,and properties of plates under the first and second rolling were compared,along with the effects of intermetallic compounds on the interface properties.The results indicate that the powder-in-tube method,when annealed at 550℃,can produce a composite plate featuring a complete and uniform Ti/Al interface.The obtained plate exhibits a peeling strength of 21.5 N/mm,tensile strength of 424 MPa,and elongation of 11.5%.Furthermore,a systematic analysis was conducted to determine the causes of performance degra-dation observed during annealing at temperatures of 600 and 650℃.

关键词： Powder-in-tube method-Ti/Al/Ti clad plate Annealing Cold rolling-Mechanical property

Research on Risk Identification and Industrial Governance of Digital Education Products Based on Data Annotation Technology

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

China Communications 2024年第3期21卷 273-282页

作者： Tong Lili zeng Jia Di ying Wang Nan Faculty of Education Beijing Normal University Beijing 100875China China Unicom Beijing 102211China

The social transformation brought aboutby digital technology is deeply impacting various industries.Digital education products, with core technologiessuch as 5G, AI, IoT (Internet of Things),etc., are continuously penetrating areas such as teaching,management, and evaluation. Apps, miniprograms,and emerging large-scale models are providingexcellent knowledge performance and flexiblecross-media output. However, they also exposerisks such as content discrimination and algorithmcommercialization. This paper conducts anevidence-based analysis of digital education productrisks from four dimensions: “digital resourcesinformationdissemination-algorithm design-cognitiveassessment”. It breaks through corresponding identificationtechnologies and, relying on the diverse characteristicsof governance systems, explores governancestrategies for digital education products from the threedomains of “regulators-developers-users”.

关键词： digital education products industry governance risk identification

H5N8亚型禽流感病毒肆虐全球,为什么中国家禽“独善其身”?

在线全文

学校读者我要写书评

暂无评论

中国预防兽医学报 2021年第12期43卷 1355-1355页

作者： CUI PENG-FEI zeng xian-ying LI XU-YONG 不详

2020年以来,H5N8亚型禽流感病毒(以下简称H5N8病毒)肆虐全球,在20多个国家引发近2 800起家禽和野鸟疫情,导致3 300多万只家禽死亡或被扑杀,给全球的家禽养殖业造成巨大经济损失。其中,处于野鸟迁徙路线的韩国和日本因疫情而扑杀的家禽... 详细信息

2020年以来,H5N8亚型禽流感病毒(以下简称H5N8病毒)肆虐全球,在20多个国家引发近2 800起家禽和野鸟疫情,导致3 300多万只家禽死亡或被扑杀,给全球的家禽养殖业造成巨大经济损失。其中,处于野鸟迁徙路线的韩国和日本因疫情而扑杀的家禽分别为1 100多万和900多万只,成为东南亚H5N8禽流感疫情的重灾区。中国比邻韩日,也是野鸟迁徙路线的途经国,而全球数量最多的中国家禽却能"独善其身"。

关键词：禽流感病毒家禽养殖业迁徙路线禽流感疫情野鸟扑杀中国家禽独善其身

Clinical observation of gastrointestinal function recovery in patients after hepatobiliary surgery

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Surgery 2024年第1期16卷 76-84页

作者： Hua-Jun zeng Jing-Jing Liu ying-Chun Yang Department of General Practice Nanyang First People's HospitalNanyang 473000Henan ProvinceChina Department of Anesthesiology Chinese People’s Armed Police Force Hospital of BeijingBeijing 100027China Department of Anesthesiology Beijing Fengtai HospitalBeijing 100071China

BACKGROUND The liver is an important metabolic and digestive organ in the human body,ca-pable of producing bile,clotting factors,and vitamins.AIM To investigate the recovery of gastrointestinal function in patients after hepato-biliary surgery and identify effective rehabilitation measures.METHODS A total of 200 patients who underwent hepatobiliary surgery in our hospital in 2022 were selected as the study subjects.They were divided into a control group and a study group based on the extent of the surgery,with 100 patients in each group.The control group received routine treatment,while the study group re-ceived targeted interventions,including early enteral nutrition support,drinking water before gas discharge,and large bowel enema,to promote postoperative gastrointestinal function recovery.The recovery of gastrointestinal function was compared between the two groups.RESULTS Compared with the control group,patients in the study group had better recovery of bowel sounds and less accumulation of fluids in the liver bed and gallbladder fossa(P<0.05).They also had shorter time to gas discharge and first meal(P<0.05),higher overall effective rate of gastrointestinal function recovery(P<0.05),and lower incidence of postoperative complications(P<0.05).CONCLUSION Targeted nursing interventions(early nutritional support,drinking water before gas discharge,and enema)can effectively promote gastrointestinal function recovery in patients undergoing hepatobiliary surgery and reduce the incidence of complications,which is worthy of promotion.

关键词： Liver and gallbladder patients Gastrointestinal function Postoperative recovery