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Significant difference between sirolimus and paclitaxel nanoparticles in antiproliferation effect in normoxia and hypoxia: The basis of better selection of atherosclerosis treatment

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Bioactive Materials 2021年第3期6卷 880-889页

作者： Youlu Chen Yong zeng Xiaowei Zhu Lifu Miao Xiaoyu Liang jianwei Duan Huiyang Li Xinxin Tian Liyun Pang Yongxiang Wei Jing Yang Tianjin Key Laboratory of Biomaterial Research Institute of Biomedical EngineeringChinese Academy of Medical Science and Peking Union Medical CollegeTianjin300192PR China Beijing Institute of Heart Lung and Blood Vessel Disease Beijing Anzhen HospitalCapital Medical UniversityBeijing100029PR China Henan Center for Patent Examination and Cooperation of the Patent Office of the State Intellectual Property Office Henan450002PR China Heart Center The First Hospital of Tsinghua UniversityBeijing100016PR China Biomedical Barriers Research Center Institute of Biomedical EngineeringChinese Academy of Medical Sciences&Peking Union Medical CollegeTianjin300192PR China

Compared with paclitaxel,sirolimus has been more used in the treatment of vascular restenosis gradually as an anti-proliferative drug,but few basic studies have elucidated its *** anti-proliferative effects of sirolimus or paclitaxel have been demonstrated by numerous studies under normoxia,but few studies have been achieved focusing *** this study,porcine carotid artery injury model and classical cobalt chloride hypoxia cell model were *** nanoparticles(SRM-NPs),paclitaxel nanoparticles(PTX-NPs)and blank nanoparticles(Blank-NPs)were prepared *** effect of RPM-NPs on the degree of stenosis,proliferative index and the expression of PCNA after 28 days of porcine carotid artery injury model was *** with saline group and SRM groups,SRM-NPs group suppressed vascular stenosis,proliferative index and the expression of PCNA(P<0.01 and P<0.05).Endothelial cell(EC)and smooth muscle cell(SMC)were pre-treated with cobaltous chloride,followed by SRM-NPs,PTX-NPs,Blank-NPs or PBS control treating,the effects on cell proliferation,HIF-1 expression and glycolysis were ***-NPs could inhibit EC and SMC proliferation under hypoxia,while PTX-NPs couldn't(P<0.001).Significant differences between sirolimus and paclitaxel NPs in anti-proliferation effect under normoxia and hypoxia may due to the different inhibitory effects on HIF-1αexpression and *** conclusion,these results suggest that sirolimus can inhibit the proliferation of hypoxic cells more effectively than *** observations may provide a basis for understanding clinical vascular stenosis therapeutic differences between rapamycin and paclitaxel.

关键词： Atherosclerosis Sirolimus Paclitaxel Hypoxia HIF-1α Glycolysis

新型抗菌肽Mt-22S3对白念珠菌细胞的作用及机制

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贵州医科大学学报 2024年第3期49卷 375-380,396页

作者：李彩多曾晔石艳萍张迎春吴建伟陈峥宏王涛贵州医科大学基础医学院贵州贵阳550025 贵州省普通高等学校病原生物学特色重点实验室贵州贵阳550025

目的探讨家蝇抗真菌肽-1A(MAF-1A)突变体-22S3(Mt-22S3)对白念珠菌(***)细胞的作用及机制。方法取对数生长期的***菌悬液,分别与0、125、250及500 mg/L Mt-22S3作用12 h,采用荧光显微技术及荧光检测法观测Mt-22S3作用***后细胞内活性氧(... 详细信息

目的探讨家蝇抗真菌肽-1A(MAF-1A)突变体-22S3(Mt-22S3)对白念珠菌(***)细胞的作用及机制。方法取对数生长期的***菌悬液,分别与0、125、250及500 mg/L Mt-22S3作用12 h,采用荧光显微技术及荧光检测法观测Mt-22S3作用***后细胞内活性氧(ROS)的变化;以荧光检测法测定Mt-22S3作用***后细胞内线粒体膜电位水平的变化;通过Annexin V-FITC/PI双染法,观察Mt-22S3对***细胞凋亡的影响;以琼脂糖凝胶电泳阻滞实验分析Mt-22S3对***细胞DNA的影响。结果经Mt-22S3作用的***细胞内ROS增高,且随着Mt-22S3浓度升高,细胞内ROS上升(P<0.05);线粒体膜电位检测发现,经Mt-22S3作用的***线粒体膜电位下降(P<0.01);Mt-22S3可引起***细胞凋亡及细胞坏死的发生,且Mt-22S3浓度越高,细胞凋亡及细胞坏死现象越明显;凝胶阻滞实验结果显示,Mt-22S3与*** DNA孵育后产生明显的凝胶阻滞现象。结论Mt-22S3可进入***细胞内发挥抗菌作用,其机制与诱导细胞内ROS积累、去极化线粒体膜、诱导细胞凋亡和死亡、结合DNA等有关。

关键词：白念珠菌活性氧细胞凋亡抗菌肽线粒体膜电位 DNA凝胶阻滞

连作烤烟土壤特征及连作障碍防控技术研究进展

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黑龙江农业科学 2024年第8期 109-117页

作者：汤宏王建伟曾掌权刘伦沛阮运飞梁春华冷丽娟凯里学院理学院贵州凯里556011 河池学院化学与生物工程学院/广西蚕桑生态学与智能化技术应用重点实验室/广西现代蚕桑丝绸协同创新中心/微生物及植物资源开发利用广西高校重点实验室广西河池546300 湖南省林业科学院/湖南衡山森林生态系统定位观测研究站湖南长沙410004

为深入阐明烤烟连作障碍的发生机制和科学选择综合防控措施,以期最大限度消减烤烟连作障碍,实现我国烤烟种植业的绿色、高效和可持续发展。从连作烤烟土壤理化性状特征、连作烤烟土壤酶活性特征、连作烤烟土壤化感物质变化特征、连作烤... 详细信息

为深入阐明烤烟连作障碍的发生机制和科学选择综合防控措施,以期最大限度消减烤烟连作障碍,实现我国烤烟种植业的绿色、高效和可持续发展。从连作烤烟土壤理化性状特征、连作烤烟土壤酶活性特征、连作烤烟土壤化感物质变化特征、连作烤烟土壤微生物群落结构和多样性变化特征等方面综述了连作烤烟土壤的特征。从培育耐连作烤烟抗性品种、优化种植制度、合理施用不同种类肥料制剂、连作土壤消毒和生物防治等方面,总结了当前我国烤烟连作障碍的防控措施,并对今后烤烟连作障碍的研究方向及防控技术最新发展方向进行了展望。

关键词：烤烟连作障碍防控技术连作土壤特征

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Highly sensitive H_(2)O_(2)-scavenging nano-bionic system for precise treatment of atherosclerosis

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Acta Pharmaceutica Sinica B 2023年第1期13卷 372-389页

作者： Xiaoyu Liang Huiyang Li Xuanling Li Xinxin Tian Aiai Zhang Qingzhi Luo jianwei Duan Youlu Chen Liyun Pang Chen Li Xing-Jie Liang Yong zeng Jing Yang Tianjin Key Laboratory of Biomaterial Research Institute of Biomedical EngineeringChinese Academy of Medical Science and Peking Union Medical CollegeTianjin 300192China Beijing Anzhen Hospital of Capital Medical University Beijing 100029China Medical College of Qinghai University Xining 810016China CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology of ChinaBejing 100190China The First Affiliated Hospital of Hebei North University Zhangjiakou 075061China First Teaching Hospital of Tianjin University of Traditional Chinese Medicine Tianjin 300381China

In atherosclerosis,chronic inflammatory processes in local diseased areas may lead to the accumulation of reactive oxygen species(ROS).In this study,we devised a highly sensitive H_(2)O_(2)-scavenging nanobionic system loaded with probucol(RPP-PU),to treat atherosclerosis more *** RPP material had high sensitivity to H_(2)O_(2),and the response sensitivity could be reduced from 40 to 10μmol/L which was close to the lowest concentration of H_(2)O_(2)levels of the pathological ***-PU delayed the release and prolonged the duration of PU in *** Apolipoprotein E deficient(ApoE-/-)mice,RPP-PU effectively eliminated pathological ROS,reduced the level of lipids and related metabolic enzymes,and significantly decreased the area of vascular plaques and *** study demonstrated that the H_(2)O_(2)-scavenging nanobionic system could scavenge the abundant ROS in the atherosclerosis lesion,thereby reducing the oxidative stress for treating atherosclerosis and thus achieve the therapeutic goals with atherosclerosis more desirably.

关键词： Highly sensitive H_(2)O_(2)-scavenging Nano-bionic system Atherosclerosis Oxidative stress Precise Target Pathological environment

东南沿海火山岩区城镇建设适宜性评价与实践——以福建省宁德市为例

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华东地质 2022年第4期43卷 490-502页

作者：王继龙林丰增彭博曾剑威武彬张艺武林韶恺孙作勇张振国于俊杰中国地质调查局南京地质调查中心江苏南京210016 宁德市自然资源局福建宁德352100 福建船政交通职业学院福建福州350007

城镇建设适宜性评价是资源环境承载能力和国土开发适宜性评价的重要内容,也是国土空间规划和管理工作的基础。以东南沿海火山岩区具有代表性的福建省宁德市为例,基于“双评价”指南构建了城镇建设适宜性评价体系,对城镇建设的适宜性及... 详细信息

城镇建设适宜性评价是资源环境承载能力和国土开发适宜性评价的重要内容,也是国土空间规划和管理工作的基础。以东南沿海火山岩区具有代表性的福建省宁德市为例,基于“双评价”指南构建了城镇建设适宜性评价体系,对城镇建设的适宜性及潜力进行评价分析,进而对城市未来发展方向及格局提出建议。宁德市城镇建设适宜区面积2682.88 km^(2),占全区陆地面积的19.98%;城镇建设不适宜区面积5979.06 km^(2),占全区陆地面积的44.53%。火山岩区受典型地貌影响,城镇建设适宜区连片性较差,分布相对零散,花岗岩区城镇建设适宜区连片度较好。适宜区展布特征及连片度整体受不同岩性区地形地貌控制,各县(区、市)差异较明显,潜力区内生态功能用地类型偏多,农村宅基地、茶园及果园地等具备城市扩展建设供给潜力。建议加强“沿东发展,向西推进”的发展方向,形成“一核-三轴-多组团”的城市发展格局,达成山海协调发展局势。

关键词：城镇建设适宜性建设潜力国土空间规划福建省宁德市东南沿海火山岩区

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Decratonic gold deposits

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Science China Earth Sciences 2015年第8期58卷 1523-1537页

作者： ZHU RiXiang FAN HongRui LI jianwei MENG QingRen LI ShengRong zeng QingDong State Key Laboratory of Lithospheric Evolution Institute of Geology and Geophysics Chinese Academy of Sciences State Key Laboratory of Geological Processes and Mineral Resources China University of Geosciences

The North China Craton(NCC) hosts numerous gold deposits and is known as the most gold-productive region of China. The gold deposits were mostly formed within a few million years in the Early Cretaceous(130–120 Ma), coeval with widespread occurrences of bimodal magmatism, rift basins and metamorphic core complexes that marked the peak of lithospheric thinning and destruction of the NCC. Stable isotope data and geological evidence indicate that ore-forming fluids and other components were largely exsolved from cooling magma and/or derived from mantle degassing during the period of lithospheric extension. Gold mineralization in the NCC contrasts strikingly with that of other cratons where gold ore-forming fluids were sourced mostly from metamorphic devolatization in compressional or transpressional regimes. In this paper, we present a summary and discussion on time-space distribution and ore genesis of gold deposits in the NCC in the context of the timing, spatial variation, and decratonic processes. Compared with orogenic gold deposits in other cratonic blocks, the Early Cretaceous gold deposits in the NCC are quite distinct in that they were deposited from magma-derived fluids under extensional settings and associated closely with destruction of cratonic lithosphere. We argue that Early Cretaceous gold deposits in the NCC cannot be classified as orogenic gold deposits as previously suggested, rather, they are a new type of gold deposits, termed as "decratonic gold deposits" in this study. The westward subduction of the paleo-West Pacific plate(the Izanagi plate) beneath the eastern China continent gave rise to an optimal tectonic setting for large-scale gold mineralization in the Early Cretaceous. Dehydration of the subducted and stagnant slab in the mantle transition zone led to continuous hydration and considerable metasomatism of the mantle wedge beneath the NCC. As a consequence, the refractory mantle became oxidized and highly enriched in large ion lithophile e

关键词： Early Cretaceous craton destruction decratonic gold deposit ore deposit model

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抗IL-17A单抗调控痛风自噬及痛风炎症的机制

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中国生物制品学杂志 2024年第3期37卷 298-305,315页

作者：王鹏黄玉琴雷天意张曾郭建伟余玲青玉凤张全波川北医学院附属医院高尿酸血症与痛风研究所四川南充637000 川北医学院附属医院四川南充637000 四川省仪陇县人民医院四川南充637000 川北医学院基础与法医学院四川南充637000

目的探讨抗IL-17A单抗(司库奇尤单抗,secukinumab)调节痛风自噬及痛风炎症的机制。方法收集57份急性期痛风(acute gout,AG)、57份间隙期痛风(intermittent gout,IG)患者和82份健康志愿者外周静脉血,RT-qPCR法检测自噬相关基因(autophagy... 详细信息

目的探讨抗IL-17A单抗(司库奇尤单抗,secukinumab)调节痛风自噬及痛风炎症的机制。方法收集57份急性期痛风(acute gout,AG)、57份间隙期痛风(intermittent gout,IG)患者和82份健康志愿者外周静脉血,RT-qPCR法检测自噬相关基因(autophagy-related gene,ATG)ATG4B、ATG7、ATG16L1、Beclin-1和微管相关蛋白1轻链3B(microtubule-associated protein 1 light chain 3B,LC3B)mRNA转录水平。在健康志愿者外周静脉血中加入单钠尿酸盐(monosodium urate,MSU)晶体,建立急性痛风炎症模型,RT-qPCR和ELISA法检测在0、1、2、4、6、8 h及不同浓度(0、100、200、400μmol/L)司库奇尤单抗作用下IL-1β转录及蛋白表达水平。将健康志愿者外周血分为对照(未经MSU处理)、MSU(100μg/mL)、MSU+秋水仙碱(100μg/mL+30μg/mL)、MSU+司库奇尤单抗(100μg/mL+400μmol/L)组,RT-qPCR和Western blot法检测IL-1β、ATG mRNA转录及蛋白表达水平,ELISA法检测IL-1β、IL-12、IL-35表达水平。结果AG、IG和健康对照组间ATG4B、Beclin-1和LC3B mRNA转录水平差异均有统计学意义(F分别为3.896、11.78、3.856,P均0.05)。结论司库奇尤单抗可调控自噬相关基因mRNA及蛋白表达,降低促炎细胞因子水平,抑制痛风炎症,为痛风治疗提供了参考。

关键词：抗IL-17A单抗司库奇尤单抗自噬痛风炎症

Corilagin inhibits SARS-CoV-2 replication by targeting viral RNA-dependent RNA polymerase

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Acta Pharmaceutica Sinica B 2021年第6期11卷 1555-1567页

作者： Quanjie Li Dongrong Yi Xiaobo Lei Jianyuan Zhao Yongxin Zhang Xiangling Cui Xia Xiao Tao Jiao Xiaojing Dong Xuesen Zhao Hui zeng Chen Liang Lili Ren Fei Guo Xiaoyu Li jianwei Wang Shan Cen Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences and Peking Union Medical SchoolBeijing 100050China Institute of Pathogen Biology Chinese Academy of Medical Sciences and Peking Union Medical SchoolBeijing 100730China Institute of Infectious Disease Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China Lady Davis Institute Jewish General HospitalMc Gill UniversityMontrealQuebec H3T 1E2Canada CAMS Key Laboratory of Antiviral Drug Research Peking Union Medical CollegeChinese Academy of Medical SciencesBeijing 100730China

Severe acute respiratory syndrome coronavirus-2(SARS-CoV-2) has become one major threat to human population *** RNA-dependent RNA polymerase(RdRp) presents an ideal target of antivirals,whereas nucleoside analogs inhibitor is hindered by the proofreading activity of ***,we report that corilagin(RAI-S-37) as a non-nucleoside inhibitor of SARS-CoV-2 RdRp,binds directly to RdRp,effectively inhibits the polymerase activity in both cell-free and cell-based assays,fully resists the proofreading activity and potently inhibits SARS-CoV-2 infection with a low 50% effective concentration(EC50) value of 0.13 μmol/*** modeling predicts that RAI-S-37 lands at the palm domain of RdRp and prevents conformational changes required for nucleotide incorporation by *** addition,combination of RAI-S-37 with remdesivir exhibits additive activity against antiSARS-CoV-2 *** with the current data available on the safety and pharmacokinetics of corilagin as a medicinal herbal agent,these results demonstrate the potential of being developed into one of the much-needed SARS-CoV-2 therapeutics.

关键词： SARS-CoV-2 RdRp Structure-based virtual screening Viral replication Non-nucleoside inhibitor Drug combinations Corilagin

Heteroheptacene-based acceptors with thieno[3,2-b]pyrrole yield high-performance polymer solar cells

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National Science Review 2022年第7期9卷 106-115页

作者： Zhenghui Luo Ruijie Ma jianwei Yu Heng Liu Tao Liu Fan Ni Jiahao Hu Yang Zou Anping zeng Chun-Jen Su U-Ser Jeng Xinhui Lu Feng Gao Chuluo Yang He Yan Shenzhen Key Laboratory of Polymer Science and Technology College of Materials Science and EngineeringShenzhen University Department of Chemistry and Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration & Reconstruction Hong Kong University of Science and Technology (HKUST) Hong Kong University of Science and Technology-Shenzhen Research Institute Department of Physics Chemistry and Biology (IFM) Link?ping University Department of Physics Chinese University of Hong Kong Synchrotron Radiation Research Center Hsinchu Science Park Department of Chemical Engineering Tsing Hua University Hong Kong University of Science and Technology (HKUST) Light-Emitting Diode and Flat Panel Display Technology Research & Development Center Hong Kong University of Science and Technology (HKUST) Foshan Research Institute for Smart Manufacturing

Rationally utilizing and developing synthetic units is of particular significance for the design of high-performance non-fullerene small-mole cule acceptors(SMAs).Here,a thieno[3,2-b]pyrrole synthetic unit was employed to develop a set of SMAs(ThPy1,ThPy2,ThPy3 and ThPy4) by changing the number or the position of the pyrrole ring in the central core based on a standard SMA of IT-4 Cl,compared to which the four thieno[3,2-b] pyrrole-based acceptors exhibit bathochromic absorption and upshifted frontier orbital energy level due to the strong ele ctron-donating ability of *** a result,the polymer solar cells(PSCs) of the four thieno[3,2-b] pyrrole-based acceptors yield higher open-circuit voltage and lower energy loss relative to those of the IT-4 Cl-based *** is more,the ThPy3-based device achieves a power conversion efficiency(PCE)(15.3%) and an outstanding fill factor(FF)(0.771) that are superior to the IT-4 Cl-based device(PCE=12.6%,FF=0.758).The ThPy4-based device realizes the lowest energy loss and the smallest optical band gap,and the ternary PSC device based on PM6:BTP-eC9:ThPy4 exhibits a PCE of 18.43% and a FF of ***,this work sheds light on the great potential of thieno[3,2-b]pyrrole-based SMAs in realizing low energy loss and high PCE.

关键词： thieno[3,2-b]pyrrole small-molecule acceptors energy loss intramolecular non-covalent interactions polymer solar cells