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H9亚型禽流感病毒M1蛋白原核表达及免疫效果初步研究

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动物医学进展 2024年第6期45卷 1-6页

作者：李小凤谢芝勋阮志华李孟李丹张民秀谢志勤罗思思韦悠谢丽基曾婷婷张艳芳黄娇玲王盛广西壮族自治区兽医研究所/广西兽医生物技术重点实验室/农业农村部中国(广西)-东盟跨境动物疫病防控重点实验室广西南宁530001

旨在原核表达H9亚型禽流感病毒(AIV)M1蛋白,初步探究其免疫效果,为研制AIV疫苗奠定基础。通过构建重组质粒pET-32a-M1,诱导表达,Western blot验证蛋白表达情况,将重组蛋白免疫接种小鼠,通过测定血清中IgG抗体效价,脾细胞培养上清多细胞... 详细信息

旨在原核表达H9亚型禽流感病毒(AIV)M1蛋白,初步探究其免疫效果,为研制AIV疫苗奠定基础。通过构建重组质粒pET-32a-M1,诱导表达,Western blot验证蛋白表达情况,将重组蛋白免疫接种小鼠,通过测定血清中IgG抗体效价,脾细胞培养上清多细胞因子分泌情况评价其免疫效果。结果显示,M1重组蛋白主要以可溶性蛋白存在,Western blot结果可见特异性条带。免疫接种小鼠后,血清产生IgG抗体,抗体效价为1∶30000。小鼠脾细胞上清液IL-2、IL-4、IL-6与对照组相比较,含量显著增加(P<0.01),而抑制IL-1β、IL-5、IL-13、IL-12p70、IL-18、GM-CMF、IFN-γ、TNF-α分泌。结果表明,M1重组蛋白免疫原性良好,刺激机体产生体液免疫和细胞免疫,为禽流感疫苗深入研究奠定了基础。

关键词：禽流感 M1蛋白原核表达免疫效果

Anti-viral activity of Staphylococcus aureus lysates against herpes simplex virus type-Ⅰinfection:an in vitro and in vivo study

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International Journal of Ophthalmology(English edition) 2021年第10期14卷 1463-1472页

作者： Tian-Lan Lin Chao Cheng Wei-ting zeng Fang Duan Yin-Hui Pei Xiu-Ping Liu Fu Shang Kai-Li Wu Zhongshan Ophthalmic Center State Key Laboratory of OphthalmologySun Yat-sen UniversityGuangzhou 510060Guangdong ProvinceChina

AIM:To investigate the effect of Staphylococcus aureus(***)lysates(SALs)on herpes simplex virus type-Ⅰ(HSV1)infection in human corneal epithelial(HCE)cells and in a mouse model of HSV1 ***:HCE,Vero,HeLa,and BV2 cells were infected with HSV1[HSV1f strain,HSV1f;HSV-1-H129 with green fluorescent protein(GFP)knock-in,HSV1g].Pre-or post-infection,SAL at various concentrations was added to the culture medium for 24 *** fluorescence in HSV1g or plaque formation by HSV1f were *** effects of heat-treated SAL,precooled acetone-precipitated SAL,and SAL subjected to ultrafiltration(100 kDa)were *** effects of other bacterial components and lysates on HSV1 infection were also tested,including lipoteichoic acid(LTA),peptidoglycan(PGN),staphylococcal protein A(SPA),andα-hemolysin from ***(α-toxin)as well as lysates from a wild-type *** strain,***,and Escherichia coli(W-SAL,SEL,and ECL,respectively).In addition,SAL eye drops were applied topically to BALB/c mice with HSV1 keratitis,followed by in vivo ***:The cytopathic effect,plaque formation(HSV1f),and GFP expression(HSV1g)in infected cells were inhibited by SAL in a dose-dependent *** active component of SAL(≥100 kDa)was heat-sensitive and retained activity after acetone *** HSV1ginfected cells,treatment with LTA-sa,α-toxin,PGN-sa,or SPA did not inhibit GFP ***,W-SAL,and SEL(but not ECL)decreased GFP *** mice with HSV1 keratitis,SAL reduced corneal lesions by 71%.CONCLUSION:The results of this study demonstrate that SAL can be used to inhibit HSV1 infection,particularly *** studies are needed to determine the active components and mechanism underlying the effects of SAL.

关键词： herpes simplex virus type-1 Staphylococcus aureus infection cornea epithelial cells keratitis

A folic acid-decorated nanoparticles loaded JQ1 for oral squamous cell carcinoma therapy

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Chinese Chemical Letters 2024年第3期35卷 332-336页

作者： Dequan zeng Zhongkai Ma Xin Zan ting Luo Xiang Wang Xiang Gao Xianghui Fu School of Health Management Xihua UniversityChengdu 610039China State Key Laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesWest China College of StomatologySichuan UniversityChengdu 610041China Department of Neurosurgery and Institute of Neurosurgery State Key Laboratory of Biotherapy and Cancer CenterWest China HospitalWest China Medical SchoolSichuan University and Collaborative Innovation Center for BiotherapyChengdu 610041China Breast Disease Center Cancer CenterWest China HospitalSichuan UniversityChengdu 610041China

Oral squamous cell carcinoma(OSCC)is known as one of the most malignant tumors with high recurrence and fatality *** poor tumor-targeting ability of traditional chemotherapeutic drugs has been a grand challenge for anti-OSCC *** that,a large quantity of tumor associated macrophages in OSCC tissues further diminish the anti-tumor effects of these ***,we produced a therapeutic nano drug delivery system(FA-PEG-PLA-JQ1)through encapsulating JQ1[a small-molecule inhibitor of bromodomain containing protein 4(BRD4)]into the folic acid(FA)-modified nanoparticle(PEG-PLA),which could prolong the half-life of JQ1 and target the tumor *** then,JQ1 released from this nanoparticle could prevent OSCC growth inducing tumor cell apoptosis,inhibiting tumor angiogenesis and the polarization of M2 type *** conclusion,our date demonstrated the therapeutic benefits of FA-PEG-PLA-JQ1 against OSCC in vivo or in vitro,which could be a novel treatment strategy for OSCC in coming days.

关键词： Folic acid Nanoparticles JQ1 Oral squamous cell carcinoma Macrophages

斑马鱼tectb内耳基因敲除品系的构建及其作用机制

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激光生物学报 2024年第2期33卷 143-150,166页

作者：刘玲朱俊伟曾婷谢缤灵陶桂芳朱贤宇谢华平湖南师范大学动物营养与人体健康实验室长沙410081

TECTB基因编码的β-盖膜蛋白(β-tectorin)在人类听觉功能中具有重要的调控作用,是内耳器官中覆盖膜的重要组成成分,在声音转导为神经信号的过程中发挥着关键作用。β-tectorin突变会导致人类非综合征性耳聋的发生,而目前关于听觉障碍... 详细信息

TECTB基因编码的β-盖膜蛋白(β-tectorin)在人类听觉功能中具有重要的调控作用,是内耳器官中覆盖膜的重要组成成分,在声音转导为神经信号的过程中发挥着关键作用。β-tectorin突变会导致人类非综合征性耳聋的发生,而目前关于听觉障碍发生的分子机制仍然有待研究。斑马鱼(Danio rerio)是一种常用的试验动物模型,能够对内耳发育和相关疾病的研究提供重要的价值。为了研究tectb基因在斑马鱼内耳发育中的作用,本研究利用CRISPR/Cas9基因编辑技术,成功构建tectb内耳基因缺失型斑马鱼模型,并对tectb基因缺失的斑马鱼表型进行初步分析。首先,在tectb基因的第三号外显子上设计靶位点以及基因型检测引物,经体外扩增得到向导DNA(sgDNA),再对其进行体外转录,得到向导RNA(sgRNA);然后,将sgRNA与Cas9酶通过显微注射的方式注射到野生型斑马鱼胚胎中,经基因型鉴定筛选出携带突变的嵌合体F0代鱼,培养至性成熟后与野生型斑马鱼杂交得到F1代突变体杂合子;在遗传稳定性鉴定及基因序列测序之后,保留突变类型为移码突变的F1代鱼,培养至成年后,经过自交得到F2代tectb突变体纯合子;最后,通过体式显微镜初步观察及毛细胞染色试验,发现突变体斑马鱼内耳组织结构正常,耳石形态和大小没有明显差异,毛细胞的发育也没有明显缺陷,但是体内器官与组织的发育是否发生变化,需要进行深入研究。这项研究为深入了解内耳发育的分子机制和耳聋等人类疾病提供了有价值的研究模型,借助tectb基因敲除斑马鱼模型,我们可以探究该基因在疾病发生中的具体作用,更好地研究内耳发育中的关键途径,有利于进一步深入研究听力的调控机制。

关键词：斑马鱼 tectb CRISPR/Cas9 内耳发育毛细胞

Campylobacter enteritis in adult patients with acute diarrhea from 2005 to 2009 in Beijing, China

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Chinese Medical Journal 2011年第10期124卷 1508-1512页

作者： CHEN Jie SUN Xin-ting zeng Zheng YU Yan-yan Department of Infectious Diseases Peking University First Hospital Beijing 100034 China

Background There has been a marked global increase in the incidence of human Campylobacter enteritis in recent years. This study investigated the epidemiological and clinical features of Campylobacter enteritis in adult patients suffering from acute *** This was a retrospective review of Campylobacter enteritis in adult patients with acute diarrhea presenting at Beijing University First Hospital, Beijing, China, in the summer and autumn （April to October） of 2005 to 2009. The data collected included the species of campylobacter identified, and the age, gender, clinical manifestations and results of laboratory test on stool samples collected from the patients. Campylobacter sensitivity tests to various antimicrobial agents were conducted on 80 specimens. Chi-square tests were applied using SPSS13.0 software and a two-sided P value of 〈0.05 was considered statistically *** Campylobacter spp. isolated from the stool specimens of 142 patients with diarrhea represented 14.9% of all the cases examined. C. jejuni was identified in 127 patients （89.4%） and C. coli in 15 others （10.6%）. The infection incidence was highest in the age range of 21-30 years which comprised 21.7% of the total cases examined. Most cases of diarrhea （46 patients） occurred in June. Watery diarrhea （97.2%）, abdominal pain （72.5%） and fever （64.8%） were the most common manifestations of enteric campylobacteriosis. Only four patients （2.8%） had bloody diarrhea. The antimicrobial resistance rates were： cefoperazone （100%）, levofloxacin （61.3%）, gentamicin （12.5%）, erythromycin （6.3%）, and azithromycin （2.5%）.Conclusions Campylobacter was prevalent among adults with acute diarrhea from 2005 to 2009 in Beijing, China. The large number of those afflicted by the disease warrants the commission of a large multicenter study to determine the extent of enteric campylobacteriosis in this region

关键词： Campylobacter enteritis drug resistance signs and symptoms

Revisit of GeV Gamma-Ray Emission from Orion B with the Fermi Large Area Telescope

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Research in Astronomy and Astrophysics 2024年第9期24卷 128-135页

作者： Wei zeng Xiao-Na Sun Rui-Zhi Yang Jia-Chun He Zhao-Dong Shi ting-ting Ge Yun-Feng Liang En-Wei Liang Guangxi Key Laboratory for Relativistic Astrophysics School of Physics Science and TechnologyGuangxi UniversityNanning 530004China Department of Astronomy School of Physical SciencesUniversity of Science and Technology of ChinaHefei 230026China CAS Key Labrotory for Research in Galaxies and Cosmology University of Science and Technology of ChinaHefei 230026China School of Astronomy and Space Science University of Science and Technology of ChinaHefei 230026China School of Physics and Astronomy Sun Yat-sen UniversityZhuhai 519082China

We revisit the γ-ray emission above 300 Me V towards the massive star-forming region of Orion B by adopting14 yr observations with the Fermi Large Area Telescope and utilizing the updated software *** extended γ-ray emission region around Orion B is resolved into two components(region Ⅰ and region Ⅱ).The γ-ray spectrum of region I agrees with the predicted γ-ray spectrum assuming the cosmic ray(CR)density is the same as that of Alpha Magnetic Spectrometer(AMS-02)measured ***γ-ray emissivity of region II appears to be deficit at low energy band(E<3 GeV).Through modeling we find that CR densities exhibit a significant deficit below 20 Ge V,which may be caused by a slow diffusion inside the dense *** is probably caused by an increased magnetic field whose strength increases with the gas density.

关键词： (ISM:)cosmic rays gamma-rays:ISM (Galaxy:)open clusters and associations:individual(NGC 2023 NGC 2024 NGC 2068 and NGC 2071)

Three new diterpenoids from Leonurus japonicus

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Chinese Chemical Letters 2014年第5期25卷 677-679页

作者： Zhi-Ke Liu Da-Rong Wu Yi-Ming Shi ting zeng Shao-Hua Liu Xue Du Yong-Jun Dang Wei-Lie Xiao Han-Dong Sun State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of Sciences University of Chinese Academy of Sciences Chengdu No. 1 Pharmaceutical Group Co. Ltd. Key Laboratory of Molecular Medicine Ministry of Educationand Department of Biochemistry and Molecular BiologyFudan University Shanghai Medical College

Phytochemical investigation of the aerial parts of Leonurus japonicus led to the isolation of one unusual clerodane diterpenoid,leojaponin A（1）,characterized by a C4–C7oxa-bridge,and two new labdane diterpenoids,leojaponins B（2） and C（3）.The structures of these new compounds were determined based on extensive 1D and 2D NMR spectroscopic *** the best of our knowledge,compound 1 is the first clerodane diterpenoid obtained from *** of them were evaluated for their cytotoxicity.

关键词： Leonurus japonicus Leojaponin A Leojaponin B Leojaponin C

Cassiterite U-Pb age in the Bawapin tin deposit, Tanintharyi Region, southern Myanmar

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China Geology 2023年第2期6卷 358-360,I0046页

作者： Song-yang Wu Shu-sheng Liu Yong-fei Yang Bin Zhang Hui-min Liang Hong Zhang Xiang-ting zeng Fei Nie Chengdu Center China Geological SurveyChengdu 610081China Applied Geological Survey Center China Geological SurveyChengdu 610036China

*** Southeast Asia has a 3500 km tin metallogenic belt in the world,extending from southern Yunnan Province to Bangka,*** region is also the world’s largest one regarding tin production,which accounts for 85%of historical tin production globally(Lehmann B,2021).Myanmar is one of the main tin producers within this region(33000 t in 2020)and has become the key supplier after China and *** on tin mineralization in Myanmar mostly focused on ore-related granites,for zircon geochronology,magma evolution,and tectonic settings(Mao W et al.,2020).

关键词： Myanmar mostly globally

Study of preoperative diagnostic modalities in Chinese patients with superficial esophageal squamous cell carcinoma

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World Journal of Gastrointestinal Surgery 2022年第9期14卷 986-996页

作者： Ya-ting zeng Yu-Ying Sun Wen-Cheng Tan Shu-Ai Luo Bi-Hui Zou Guang-Yu Luo Chun-Yu Huang State Key Laboratory of Oncology in South China Sun Yat-sen University Cancer CenterGuangzhou 510060Guangdong ProvinceChina Collaborative Innovation Center for Cancer Medicine Sun Yat-sen University Cancer CenterGuangzhou 510060Guangdong ProvinceChina Department of Endoscopy Sun Yat-sen University Cancer CenterGuangzhou 510060Guangdong ProvinceChina Cancer Prevention Center Sun Yat-sen University Cancer CenterGuangzhou 510060Guangdong ProvinceChina

BACKGROUND Endoscopic ultrasonography(EUS)and magnifying endoscopy(ME)reliably determine indications for endoscopic resection in patients with superficial esophageal squamous cell carcinoma(SESCC).ME is widely accepted for predicting the invasion depth of superficial esophageal cancer with satisfying ***,the addition of EUS is *** To evaluate the diagnostic efficiency of ME vs EUS for invasion depth prediction and investigate the influencing factors in patients with SESCC to determine the best diagnostic model in *** We retrospectively analyzed patients with suspected SESCC who completed both ME and EUS and then underwent endoscopic or surgical resection at Sun Yat-Sen University Cancer Center between January 2018 and December *** evaluated and compared the diagnostic efficiency of EUS and ME according to histological results,and investigated the influencing *** We included 152 lesions from 144 patients in this *** diagnostic accuracies of ME and EUS in differentiating invasion depth were not significantly different(73.0%and 66.4%,P=0.24);both demonstrated moderate consistency with the pathological results(ME:kappa=0.58,95%confidence interval[CI]:0.48-0.68,P<0.01;EUS:kappa=0.46,95%CI:0.34-0.57,P<0.01).ME was significantly more accurate in the diagnosis of high-grade intraepithelial(HGIN)or carcinoma in situ(odds ratio[OR]=3.62,95%CI:1.43-9.16,P=0.007)*** a miniature probe rather than conventional EUS can improve the accuracy of lesion depth determination(82.3%vs 49.3%,P<0.01).Less than a quarter of circumferential occupation and application of a miniature probe were independent risk factors for the accuracy of tumor invasion depth as assessed by EUS(<1/4 circumferential occupation:OR=3.07,95%CI:1.04-9.10;application of a miniature probe:OR=5.28,95%CI:2.41-11.59,P<0.01).Of the 41 lesions(41/152,27.0%)that were misdiagnosed by ME,24 were corrected by EUS(24/41,58.5%).CONCLUSION Preoperative diagnos

关键词： Superficial esophageal squamous cell carcinoma Endoscopic ultrasound Magnifying endoscopy Endoscopic resection Japan Esophageal Society classification