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Epidemiology and immunodiagnostics of Strongyloides stercoralis infections among migrant workers in Malaysia

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Asian Pacific Journal of Tropical Medicine 2019年第6期12卷 250-257页

作者： Norhidayu Sahimin yvonne A.L.Lim Rahmah Noordin Muhammad Hafiznur Yunus Norsyahida Arifin Jerzy Marian Behnke Siti Nursheena Mohd Zain Tropical Infectious Diseases Research and Education Centre(TIDREC) University of Malaya50603 Kuala LumpurMalaysia Department of Parasitology Faculty of MedicineUniversity of MalayaKuala Lumpur.Malaysia Institute for Research in Molecular Medicine(INFORMM) Universiti Sains Malaysia11800 Pulau PinangMalaysia School of Life Sciences University of NottinghamUniversity ParkNottinghamNG7 2RDUK Institute of Biological Science Faculty of ScienceUniversity of MalayaKuala LumpurMalaysia

Objective: To investigate the status of Strongyloides(S.) stercoralis infections among migrant workers in Malaysia for the first time and identify risk ***: Four diagnostic methods were employed for the detection of S. stercoralis including microscopy, enzyme-linked immunosorbent assay(ELISA) using a commercial kit, ELISA using the rSs1a antigen and polymerase chain reaction(PCR). Low and semi-skilled workers from five working sectors(i.e. manufacturing, food service, agriculture and plantation, construction and domestic service) were tested on a voluntary basis. Results: The overall seroprevalence of S. stercoralis from 483 workers employing the ELISA commercial kit for IgG was 35.8%(n=173;95% CI: 31.5%-40.1%) whereas seroprevalence using the rSs1a-ELISA was 13.0%(n=63;95% CI: 10.0%-16.0%). Cross tabulation between the ELISA commercial kit and rSs1a-ELISA showed that only 6.4%(n=31;95% CI: 4.2%-8.6%) of the samples were positive in both tests. Microscopic examination of all 388 fecal samples were negative;however subsequent testing by a nested PCR against DNA from the same samples successfully amplified DNA from three male subjects(0.8%;3/388). Male workers, India and Myanmar nationality, food service occupation and those living in the hostel were statistically significant with seroprevalence(P<0.005). Conclusion: This is the first report on the epidemiology of S. stercoralis infections among the migrant workers in Malaysia. Our results highlight the importance of using appropriate diagnostic tools for detection. The presence of anti-S. stercoralis antibodies in the study population calls for improvements in personal hygiene and sanitation standards among migrant workers in Malaysia through control strategies including health education campaigns and programs aimed at increasing awareness and healthy behaviors.

关键词： Strongyloides stercoralis Migrant workers Microscopic examination Serological analysis PCR

Comparison of bacterial quantities in left and right colon biopsies and faeces

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World Journal of Gastroenterology 2012年第32期18卷 4404-4411页

作者： Anna Lyra Sofia Forssten Peter Rolny yvonne Wettergren Sampo J Lahtinen Krista Salli Lennart Cedgrd Elisabeth Odin Bengt Gustavsson Arthur C Ouwehand DuPont Nutrition and Health KantvikActive Nutrition02460 KantvikFinland Department of Medicine Sahlgrenska AcademyUniversity of GothenburgS-41685 GothenburgSweden Wasa MedicalsAB Probiotic DivisionS-302 91 HalmstadSweden Department of Surgery Sahlgrenska AcademyUniversity of GothenburgS-41685 GothenburgSweden

AIM:To compare quantities of predominant and pathogenic bacteria in mucosal and faecal ***:Twenty patients undergoing diagnostic colonoscopy with endoscopically and histologically normal mucosa were recruited to the study,14 subjects of which also supplied faecal(F) samples between 15 d to 105 d post *** biopsies were taken from each subject from the midportion of the ascending colon(right side samples,RM) and the sigmoid(left side samples,LM).Predominant intestinal and mucosal bacteria including clostridial 16S rRNA gene clusters Ⅳ and ⅩⅣab,Bacteroidetes,Enterobacteriaceae,Bifidobacterium spp.,Akkermansia muciniphila(***),Veillonella spp.,Collinsella spp.,Faecalibacterium prausnitzii(***) and putative pathogens such asEscherichia coli(***),Clostridium difficile(***),Helicobacter pylori(***) and Staphylococcus aureus(***) were analysed by quantitative polymerase chain reaction(qPCR).Host DNA was quantified from the mucosal samples with human glyceraldehyde 3-phosphate dehydrogenase gene targeting *** t tests and the Pearson correlation were applied for statistical ***:The most prominent bacterial groups were clostridial groups Ⅳ and ⅩⅣa+b andBacteroidetes and bacterial species *** in both sample *** and *** were not detected and *** was detected in only one mucosal sample and three faecal *** was detected in less than half of the mucosal samples at both sites,but was present in all faecal *** detected bacteria,except Enterobacteriaceae,were present at higher levels in the faeces than in the mucosa,but the different locations in the colon presented comparable quantities(RM,LM and F followed byP 1 for RMvs F,P 2 for LMvs F andP 3 for RM vs LM:4.17 ± 0.60 log 10 /g,4.16 ± 0.56 log 10 /g,5.88 ± 1.92 log 10 /g,P 1 = 0.011,P 2 = 0.0069,P 3 = 0.9778 ***;6.25 ± 1.3 log 10 /g,6.09 ± 0.81 log 10 /g,8.84 ± 1.38 log 10 /g,P

关键词： Gastrointestinal microbiota Mucosa Faeces Real-time quantitative polymerase chain reaction Sampling

Immune and microRNA responses to Helicobacter muridarum infection and indole-3-carbinol during colitis

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World Journal of Gastroenterology 2020年第32期26卷 4763-4785页

作者： Rasha Raheem Alkarkoushi yvonne Hui Abbas S Tavakoli Udai Singh Prakash Nagarkatti Mitzi Nagarkatti Ioulia Chatzistamou Marpe Bam Traci L Testerman Department of Pathology Microbiology and ImmunologyUniversity of South Carolina School of MedicineColumbiaSC 29209United States College of Nursing University of South CarolinaUniversity of South CarolinaColumbiaSC 29208United States Department of Medicine Hematology and OncologyUniversity of Virginia School of MedicineCharlottesvilleVA 22908United States

BACKGROUND Indole-3-carbinol(I3C)and other aryl hydrocarbon receptor agonists are known to modulate the immune system and ameliorate various inflammatory and autoimmune diseases in animal models,including colitis induced by dextran sulfate sodium(DSS).MicroRNAs(miRNAs)are also gaining traction as potential therapeutic agents or diagnostic *** Helicobacter(EHH)species are associated with an increased risk of inflammatory bowel disease,but little is known about how these species affect the immune system or response to *** To determine whether infection with an EHH species alters the response to I3C and how the immune and miRNA responses of an EHH species compare with responses to DSS and inflammatory bowel *** We infected C57BL/6 mice with Helicobacter muridarum(***),with and without DSS and I3C *** responses were evaluated by histological examination,symptom scores,and cytokine *** analysis was performed on mesenteric lymph nodes to further evaluate the regional immune *** *** infection alone caused colonic inflammation and upregulated proinflammatory,macrophage-associated cytokines in the colon similar to changes seen in DSS-treated *** upregulation occurred upon treatment with *** infection caused broad changes in mesenteric lymph node miRNA expression,but colitis-associated miRNAs were regulated similarly in ***-infected and uninfected,DSS-treated *** spite of causing colitis exacerbation,*** infection did not prevent disease amelioration by I3C.I3C normalized both macrophage-and T cell-associated *** Thus,I3C may be useful for inflammatory bowel disease patients regardless of EHH *** miRNA changes associated with I3C treatment are likely the result of,rather than the cause of immune response changes.

关键词： Helicobacter muridarum MicroRNA Immune T regulatory cell T helper 17 cell Colitis Cytokine

Fatal case of amoebic liver abscess in a child

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Asian Pacific Journal of Tropical Medicine 2015年第10期8卷 858-860页

作者： Khuen Foong Ng Kah Kee Tan Romano Ngui yvonne AL Lim Amirah Amir Yamuna Rajoo Hamimah Hassan Rohela Mahmud Department of Pediatric Tuanku Jaafar Hospital Department of Parasitology Faculty of MedicineUniversity of Malaya Department of Medical Microbiology Faculty of MedicineUniversity of Malaya

We reported a case of amoebic liver abscess(ALA) in a 6-year-old Malaysian boy who presented with fever,lethargy,diarrhea and right hypochondriac *** admission he was diagnosed with perforated acute appendicitis and a laparotomy was *** surgery he developed acute respiratory ***,chest X-Ray and CT scan revealed two ALAs in the posterior segment of right lobe of liver,pleural effusion and collapsed consolidation of lungs *** liver abscesses drainage was done and intravenous Metronidazole was *** carried out on the pus from the abscess was positive for Entamoeba *** however succumbed to the infection one week after admission.

关键词： Amoebic liver abscess Entamoeba histolytica Childr

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产出绩效评估对图图梅区小学学习表现影响

产出绩效评估对图图梅区小学学习表现影响

作者： yvonne Makwaeba 华东师范大学

学位级别：硕士

该研究调查了产出绩效评估对图图梅(Tutume)区小学学习表现的影响。本研究使用了目的性抽样和随机抽样两种抽样方法。本研究的目标人群包含了图图梅区小学的教学人员,包括蒂尼(Thini)、马加帕托纳(Magapatona)、图图梅中心小学,目标人数... 详细信息

该研究调查了产出绩效评估对图图梅(Tutume)区小学学习表现的影响。本研究使用了目的性抽样和随机抽样两种抽样方法。本研究的目标人群包含了图图梅区小学的教学人员,包括蒂尼(Thini)、马加帕托纳(Magapatona)、图图梅中心小学,目标人数为50人。这些教学人员是课程实施者,通过实行绩效评估而达到相应的学习表现。研究者利用伊图腾(Ithuteng)小学的教学人员进行试点研究以测试研究工具。这所学校也在同一个基础教育部的领导之下,因此,其所使用的教学策略及其审查原则与来自图图梅区小学的研究目标相似。研究者以问卷调查为研究方法。研究发现,对产出绩效评估在改善教学和学习方面起着非常关键的作用。研究指出,如果付出了应有的努力,必然能取得成功。通过在学科层面上进行绩效评估,并在之后将其推广到整个学校层面,学习表现将得以提升。尽管调查结果对绩效评估表示了肯定,但受访者提出了一些与之相反的观点。受访者认为这种做法不是为了带来积极的变化,而是为了取悦他们的雇主。

关键词：绩效评估产出学习表现

Social integration: How is it related to self-rated health?

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Advances in Aging Research 2013年第1期2卷 10-20页

作者： Hejun Kang yvonne L. Michael Department of Epidemiology and Biostatistics Drexel University Philadelphia USA Department of Geography University of Idaho Moscow USA

Social integration has well-established health benefits among older adults in observational studies. However, interventions designed to increase social integration have not improved health suggesting important knowledge gaps on how social integration influences health outcomes. This study developed a new measure of social integration, daily social contact, capturing the interpersonal nature of social integration and mobility of individuals, and providing a direct assessment of individuals’ real-time access to companionship and social support. The data used is the 2006-2007 American Time Use Survey (ATUS), which surveyed 25,191 individuals aged 15 years and older (n = 4378 aged 65 years and older). Generalized ordinal logistic regression analyses revealed positive, but non-parallel relationships between daily social contacts and the ordinal categories of self-rated health among older adults. This study may be used to identify populations that experience social exclusion, such that future research can determine more precisely how to intervene to improve health outcomes.

关键词： Daily Social Contact Social Integration Self-Rated Health Older Adults Generalized Ordinal Logistic Regression

A Highly Efficient Agrobacterium-Mediated Method for Transient Gene Expression and Functional Studies in Multiple Plant Species

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Plant Communications 2020年第5期1卷 129-140页

作者： Youjun Zhang Moxian Chen Beata Siemiatkowska Mitchell Rey Toleco Yue Jing Vivien Strotmann Jianghua Zhang yvonne Stahl Alisdair R.Fernie Center of Plant Systems Biology and Biotechnology 4000 PlovdivBulgaria Max-Planck-Institut fur Molekulare Pflanzenphysiologie Am Muhlenberg 114476 Potsdam-GolmGermany Department of Biology Hong Kong Baptist Universityand State Key Laboratory of AgrobiotechnologyThe Chinese University of Hong KongShatinHong Kong Institute for Developmental Genetics Heinrich Heine UniversityDusseldorfGermany

Although the use of stable transformation technology has led to great insight into gene function,its application in high-throughput studies remains ***-infiltration have been widely used in species such as Nicotiana benthamiana for the rapid detection of gene expression and protein interaction analysis,but this technique does not work efficiently in other plant species,including Arabidopsis *** an efficient high-throughput transient expression system is currently lacking in the model plant species ***,we developed a method that is characterized by high efficiency,reproducibility,and suitability for transient expression of a variety of functional proteins in *** and 7 other plant species,including Brassica oleracea,Capsella rubella,Thellungiella salsuginea,Thellungiella halophila,Solanum tuberosum,Capsicum annuum,and *** of this method was independently verified in three independent research facilities,pointing to the robustness of this ***,in addition to demonstrating the utility of this technique in a range of species,we also present a case study employing this method to assess protein–protein interactions in the sucrose biosynthesis pathway in Arabidopsis.

关键词： transient expression agro-infiltration subcellular localization protein-protein interaction

Impact of humic acids on the colonic microbiome in healthy volunteers

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World Journal of Gastroenterology 2017年第5期23卷 885-890页

作者： Alexander Swidsinski yvonne Dorffel Vera Loening-Baucke Christoph Gille Anne Reiβhauer Onder Goktas Monika Krüger Jürgen Neuhaus Wieland Schrodl Laboratory for Molecular Genetics Polymicrobial Infections and BiofilmsDepartment of MedicineSection of GastroenterologyHepatology and EndocrinologyCharitéUniversitatsmedizin Berlin Outpatient Clinic Luisenstr.11-13CharitéUniversitatsmedizin Berlin entre for Infectious Diseases Faculty of Veterinary MedicineUniversity of Leipzig

AIMTo test the effects of humic acids on innate microbial communities of the *** followed the effects of oral supplementation with humic acids (Activomin®) on concentrations and composition of colonic microbiome in 14 healthy volunteers for 45 d. 3 × 800 mg Activomin® were taken orally for 10 d followed by 3 × 400 mg for 35 d. Colonic microbiota were investigated using multicolor fluorescence in situ hybridization (FISH) of Carnoy fixated and paraffin embedded stool cylinders. Two stool samples were collected a week prior to therapy and one stool sample on days 10, 31 and 45. Forty-one FISH probes representing different bacterial groups were *** sum concentration of colonic microbiota increased from 20% at day 10 to 30% by day 31 and remained stable until day 45 (32%) of humic acid supplementation (P 0.05). The others remained unaffected. Bacterial groups with initially marginal concentrations (9/mL) demonstrated no response to humic acids. The concentrations of pioneer groups of Bifidobacteriaceae, Enterobacteriaceae and Clostridium difficile increased but the observed differences were statistically not *** acids have a profound effect on healthy colonic microbiome and may be potentially interesting substances for the development of drugs that control the innate colonic microbiome.

关键词： Fluorescence in situ hybridization Colonic microbiota Colonic bioreactor Humic acids Healthy volunteers Oral supplementation

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采用水墨为环保做贡献

印刷技术 2000年第7期 29-30页

作者： yvonne Krol 林华

近年来,欧洲各国的环保法规越来越严格,法规中对印刷业VOC(挥发性有机化合物)的排放更有明确限制。因此很多公司为了遵守法规,对自己的工厂或工艺采取了相应的改进措施。荷兰Polarcup公司是Huhtamaki Van Leer集团的重要组成部分,主要... 详细信息

近年来,欧洲各国的环保法规越来越严格,法规中对印刷业VOC(挥发性有机化合物)的排放更有明确限制。因此很多公司为了遵守法规,对自己的工厂或工艺采取了相应的改进措施。荷兰Polarcup公司是Huhtamaki Van Leer集团的重要组成部分,主要产品是纸杯及硬塑料杯。Polarcup公司日产纸杯300万个,其中有1/3是用BHS四色宽幅柔印机印刷的,其余纸杯则用一台Kochsiek六色凹印机、一台KBA Planeta单张纸五色胶印机及一台三菱单张纸六色胶印机印刷。据Polarcup公司印刷部经理Helma Arink介绍。

关键词：印刷水墨环保油墨清洗工作