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Elevated free cholesterol in a p62 overexpression model of non-alcoholic steatohepatitis

World Journal of Gastroenterology 2014年第47期20卷 17839-17850页

作者： yvette simon Sonja M Kessler Katja Gemperlein Rainer M Bohle Rolf Müller Johannes Haybaeck Alexandra K Kiemer Department of Pharmacy Pharmaceutical BiologySaarland University66123 SaarbrückenGermany Medical University of Graz Institute of PathologyGraz 8036Austria Department of Pharmacy Pharmaceutical BiotechnologySaarland University and Helmholtz Institute for Pharmaceutical Research Saarland (HIPS)Helmholtz Centre for Infection Research (HZI)66123 SaarbrückenGermany Saarland University Department of Pathology66421 HomburgGermany

AIM: To characterize how insulin-like growth factor 2 (IGF2) mRNA binding protein p62/IMP2-2 promotes steatohepatitis in the absence of dietary cholesterol.

关键词： Insulin-like growth factor 2 mRNA binding protein 2-2 Methionine/choline deficient Non-alcoholic fatty liver disease Filipin Iron

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Rapid chromatographic method to decipher distinct alterations in lipid classes in NAFLD/NASH

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World Journal of Hepatology 2013年第10期5卷 558-567页

作者： Stephan Laggai yvette simon Theo Ranssweiler Alexandra K Kiemer Sonja M Kessler Department of Pharmacy Pharmaceutical Biology Saarland University

AIM: To establish a simple method to quantify lipid classes in liver diseases and to decipher the lipid profile in p62/IMP2-2/IGF2BP2-2 transgenic ***: Liver-specific overexpression of the insulin-like growth factor 2 mRNA binding protein p62/IMP2-2/IGF2BP2-2 was used as a model for *** was induced by feeding a methioninecholine deficient diet. Steatosis was assessed histologically. For thin layer chromatographic analysis, lipids were extracted from freeze-dried tissues by hexane/2-propanol, dried, redissolved, and chromatographically separated by a two-solvent system. Dilution series of lipid standards were chromatographed, detected, andquantified. The detection was performed by either2',7'-dichlorofluoresceine or a sulfuric acid/ethanol ***: Histological analyses confirmed steatosis and steatohepatitis development. The extraction,chromatographic, and detection method showed high inter-assay reproducibility and allowed quantification of the different lipid classes. The analyses confirmed an increase of triglycerides and phosphatidylethanolamine and a decrease in phosphatidylcholine in the methionine-choline deficient diet. The method was used for the first time to asses the lipid classes induced in the p62-overexpressing mouse model and showed a significant increase in all detected lipid species with a prominent increase of triglycerides by 2-fold. Interestingly, the ratio of phosphatidylcholine to phosphatidylethanolamine was decreased, as previously suggested as a marker in the progression from steatosis to ***: The thin layer chromatography analysis allows a reliable quantification of lipid classes and provides detailed insight into the lipogenic effect of p62.

关键词： Non alcoholic steatohepatitis Non alcoholic fatty liver disease Thin layer chromatography IMP2/IGF2BP2 p62 Methionine choline deficient diet Polar lipids neutral lipids Phosphatidylcholine/phosphatidylethanolamine ratio Triglycerides

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Anticonvulsant Effects of Chrysanthellum americanum L. (Vatke) Aqueous Extract in Mice Pilocarpine Model of Epilepsy and Associated Memory Impairment: Role of Antioxidant Defense System and Cholinergic Transmission

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Journal of Biosciences and Medicines 2023年第6期11卷 81-102页

作者： yvette Nguezeye Fanta Sabine Adeline Yadang simon Pale Vanessa Tita Jugha Hart Mann Alain Youbi Mambou Raymond Bess Bila Tambong Ako Ojongnkpot Germain Sotoing Taiwe Gabriel Agbor Agbor Elisabeth Ngo Bum Centre for Research on Medicinal Plants and Traditional Medicine Institute of Medical Research and Medicinal Plants Studies Yaoundé Cameroon Department of Biological Sciences Faculty of Science University of Ngaoundere Ngaoundéré Cameroon Department of Animal Biology and Conservation Faculty of Science University of Buea Buea Cameroon

Chrysanthellum americanum (L.) Vatke is a medicinal plant used by the traditional healers to treat epilepsy and associated memory impairment. This work aims at evaluating the anticonvulsant effects of Chrysanthellum americanum aqueous extract in mice pilocarpine model of epilepsy and associated memory loss. Mice were administered orally Chrysanthellum americanum aqueous extract (27.69, 69.22, 138.45, 276.9 mg/kg, prepared from the whole part) for test groups, intraperitoneally 300 mg/kg sodium valproate for the positive control group or orally 10 mL/kg distilled water for the negative control group, respectively, during a period of seven consecutive days. On the first day, temporal lobe epilepsy was induced by intraperitoneal injection of 360 mg/kg pilocarpine one hour after the administration of different treatment to mice, and the occurrence of status epilepticus was evaluated. On the second day, the anticonvulsant property was measured after the intraperitoneal injection of a sub-convulsive dose of picrotoxin (1 mg/kg). On the seventh day, the anti-amnesic properties of the extract were evaluated in the epileptic mice using the T-maze and open field paradigms. The results show that Chrysanthellum americanum extract significantly (p Chrysanthellum americanum (276.9 mg/kg) likewise sodium valproate (300 mg/kg) significantly (p Chrysanthellum americanum aqueous extract has anticonvulsant effects against pilocarpine induced-epileptic seizures and memory impairment. These properties could be mediated by the amelioration of antioxidant defense system and cholinergic neurotransmission in epileptic mice, which could partly justify the use of Chrysanthellum americanum in the traditional medicine for the treatment of epilepsy.

关键词： Chrysanthellum americanum Epilepsy Memory Impairment Oxidative Stress Cholinergic Transmission

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新研究确定了与肝病和脂肪代谢有关的肝细胞突变

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中西医结合肝病杂志 2021年第11期31卷 1011-1011页

作者： Stanley W. K. Ng Foad J. Rouhani simon F. Brunner Natalia Brzozowska Federico Abascal Luiza Moore Lia Chappell Daniel Leongamornlert Aleksandra Ivovic Philip Robinson Timothy Butler Mathijs A. Sanders Nicholas Williams Tim H. H. Coorens Jon Teague Keiran Raine Adam P. Butler yvette Hooks Beverley Wilson Natalie Birtchnell Michael R. Stratton Iñigo Martincorena Raheleh Rahbari Peter J. Campbell Foad J. Rouhani & Huw Naylor Sarah J. Aitken & Matthew Hoare Sarah J. Aitken & Susan E. Davies Sarah J. Aitken Ming Yang Efterpi Nikitopoulou Christian Frezza Matthew Hoare Cancer Genome Project Wellcome Sanger Institute Hinxton UK Department of Hematology Erasmus University Medical Center Rotterdam The Netherlands Stem Cell Institute University of Cambridge Cambridge UK Department of Surgery Addenbrooke’s Hospital Cambridge UK CRUK Cambridge Institute Cambridge UK Department of Pathology Addenbrooke’s Hospital Cambridge UK MRC Toxicology Unit University of Cambridge Cambridge UK MRC Cancer Unit University of Cambridge Cambridge UK Department of Medicine University of Cambridge Addenbrooke’s Hospital Cambridge UK

一项来自英国韦尔科姆基金会桑格研究所和剑桥大学等研究机构的研究人员的新研究分析了来自34例患者肝脏样本的1590个基因组,包括健康对照、酒精相关肝病和非酒精性脂肪肝疾病患者的肝脏样本,结果发现ACVR2A、TNRC6B、NEAT1、FOXO1和GP... 详细信息

一项来自英国韦尔科姆基金会桑格研究所和剑桥大学等研究机构的研究人员的新研究分析了来自34例患者肝脏样本的1590个基因组,包括健康对照、酒精相关肝病和非酒精性脂肪肝疾病患者的肝脏样本,结果发现ACVR2A、TNRC6B、NEAT1、FOXO1和GPAM 五个基因在肝病患者的肝细胞中发生了突变,过程如图a所示。而其中的FOXO1、CIDEB、GPAM三个基因是对肝细胞如何代谢脂肪和对胰岛素作出反应有直接影响的基因。

关键词： FOXO1 肝病患者细胞突变肝细胞脂肪代谢胰岛素 GPA 剑桥大学

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