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Detection of Low-Risk and High-Risk Oncogenic Human Papillomavirus in Archived Tissues from ENT Tumors in Burkina Faso

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Journal of Biosciences and Medicines 2023年第9期11卷 171-180页

作者： Ali Kandé Maïmouna Ilboudo Djénéba Ouermi Ina marie Angèle Traore Abdoul Karim Ouattara Théodora Mahoukèdè Zohoncon Esther Mah Alima Traore Florencia W. Djigma Dorcas Obiri-Yeboah Henriette Poaty yvette marie chantal gyebre Olga M. Lompo Charlemagne MR Ouedraogo Jacques Simpore Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Laboratoire de Biologie moléculaire et de génétique (LABIOGENE) UFR/SVT Université Joseph Ki-Zerbo Ouagadougou Burkina Faso University Post Office Department of Microbiology and Immunology School of Medical Sciences University of Cape Coast Cape Coast Ghana Centre Hospitalier Universitaire Yalgado Ouedraogo Université Joseph Ki-Zerbo Ouagadougou Burkina Faso

Human papillomavirus (HPV) is classified into high-risk HPV (HR-HPV) and HPV (LR-HPV) according to their oncogenic potential. These viruses can be found in the cervix, vagina, vulva, anus and in the ENT sphere. HPV ENT infections can lead to benign or malignant tumors in which we could find both LR-HPV and HR-HPV genotypes. The objective of this study was to investigate the genotypes of HR-HPV and LR-HPV in archived tissue samples derived from both benign and malignant tumors of the ear, nose, and throat (ENT) in Ouagadougou, Burkina Faso. One hundred and twenty formalin-fixed, paraffin-embedded archived tissues of the ENT sphere from 26 benign tumors and 94 malignant tumors were included. The tissues were first deparaffinized with xylem. The extracted DNA was used to test for high-risk and low-risk HPV by Real-Time Multiplex PCR. HPV DNA was found in 57.7% (15/26) of benign tumors and 43.61% (41/94) of malignant tumors. The prevalence of HPV infection was 46.67% (56/120) in all tumors combined. The most common HPV genotypes found were HPV 11 (34.28%), HPV 6 (30%), HPV56 (14.28%) and HPV 33 (8.57%). There were 21.43% (12/56) cases of genotypes co-infections with 10 cases of double infection and 2 cases of triple infection. Both low-risk and high-risk HPV are found in ENT tumors with relatively high HPV prevalence.

关键词： HPV Archived Tissues ENT Tumor Burkina Faso

Management of Vulnating Esophageal Foreign Bodies in Burkina Faso

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International Journal of Otolaryngology and Head & Neck Surgery 2024年第2期13卷 135-148页

作者： Ibrahima Diallo Aboubacar Gouéta Alseny Camara Assoumi Anatou Biga Mama Brigitte Ouoba Edi Emmanuel Martial Nao Moustapha Sérémé Bertin Priva Ouédraogo yvette marie chantal Gyébré Abdoulaye Keita Alpha Oumar Diallo ENT and HNS Department Yalgado Ouédraogo University Hospital Ouagadougou Burkina Faso ENT and HNS Department Bogodogo University Hospital Ouagadougou Burkina Faso ENT and HNS Department Tengandogo University Hospital Ouagadougou Burkina Faso ENT and HNS Department Conakry University Hospital Conakry Guinea

Introduction: Esophageal foreign bodies (EFB) are a diagnostic and therapeutic emergency because of the serious complications they can cause. Aim: This paper aimed to study the vulnating esophageal foreign bodies in the ENT and Head and Neck Surgery departments of the Yalgado Ouedraogo and Bogodogo University Hospital. Methodology: This was an analytic cross-sectional study with retrospective data collection over 10 years (2012-2021). Results: We collected 91 cases of vulnating esophageal foreign bodies, i.e. 9.1 cases/year (4.7%). The mean age of the patients was 14 ± 19 years. The sex ratio was 1.6. The circumstances of occurrence were dominated by accidental ingestion of vulnating esophageal foreign bodies (98.9%). The average time to consultation was 7.5 hours. Dysphagia was the dominant symptom (64.8%). Cervico-thoracic radiography found dual contour radiopaque images in 71.4%. Esophagoscopy with rigid tube was performed in 97.8%. The average time for extraction of the vulnating esophageal foreign bodies was 8 hours. Vulnerating esophageal foreign bodies were non-organic in 84.6%. The button cell represented 64.8%. Their location was cervical in 61.5% intraoperatively. The lesion assessment found ulcerative lesions in 42.9% (p Conclusion: Vulnating esophageal foreign bodies are relatively frequent in our ENT practice. Although their diagnosis is often easy, their treatment is still difficult and requires multidisciplinary management. Thus, for us, prevention remains the first effective weapon.

关键词： Foreign Bodies Esophagus Vulnerable Management Sequelae

Foreign Body Larynx in ORL Department of University Hospital-Yalgado Ouedraogo

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International Journal of Otolaryngology and Head & Neck Surgery 2016年第3期5卷 129-133页

作者： yvette marie chantal Gyé bré Noé Zaghré Aboubacar Goué ta Maimouna Ouattara Kampadilemba Ouoba Service d’ORL et de CC-F CHU-YO Ouagadougou Burkina Faso Service d’ORL et de CC-F Blaise Compaoré Ouagadougou Burkina Faso

Introduction: Foreign bodies of the larynx are serious. They can be immediately life-threatening. The aim of this study was to report the epidemiological and clinical features that characterize this affection. Material and method: We retrospectively reviewed the clinical records of patients admitted to foreign bodies of the lower airways in the Otorhinolaryngology (ORL) department of the University Hospital-Yalgado Ouedraogo (UH-YO) from 2008 to 2012. Results: Seventeen (17) foreign body records were compiled on a group of 76 foreign bodies of the lower airways. The average age was 5 years. Patients ranged in age from 6 months to 35 years with a sex ratio of 1.4. The call signs were the penetration syndrome that was found in 4 cases (23.5%), laryngeal dyspnea was found in 8 cases (47.05%) with stage II to III and dysphonia 10 cases (58.8%). The extraction was done endoscopically in all patients, and a tracheostomy was made in 2 cases (11.7%). A death was noted in 1 case (5.9%). Conclusion: The laryngeal foreign bodies are common and maintain their classical gravity. It should be thought about in front of any dyspnea associated with dysphonia in children previously healthy. The prevention is the key weapon in our context.

关键词： Larynx Foreign Body Endoscopy

Complications of Cervicofacial Cellulitis Supported in University Hospital Yalgado Ouedraogo

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International Journal of Otolaryngology and Head & Neck Surgery 2016年第3期5卷 115-120页

作者： yvette marie chantal Gyé bré Aboubacar Goué ta Noé Zaghré Moustapha Sé ré mé Bertin Priva Oué draogo Kampadilemba Ouoba Service d’ORL et de CC-F CHU-YO Ouagadougou Burkina Faso Service d’ORL et de CC-F Blaise Compaoré Ouagadougou Burkina Faso

Introduction: The complications of cervicofacial cellulitis are one of the most serious emergencies Oto-Rhino-Laryngology (ORL). These complications are still observed in our context despite the advent of antibiotics. The aim of our study was to describe the clinical presentation and management of patients admitted to our institution for complications of cervicofacial cellulitis. Patients and Methods: It came from a retrospective study in the ORL service, a department of Yalgado Ouédraogo Hospital of Ouagadougou, between January 2005 and December 2014, during which all patients with cervicofacial cellulitis complications were identified. Results: We collected over 10 years, 69 cases of complicated cellulitis, a frequency of 54.3% of cervicofacial cellulitis and 2.3% of all hospitalizations. The group included 33% women and 67% men to 29 years of average age. The non-steroidal anti-inflammatory isolated was the main factor contributing 59.4%. The front door was mainly dental 43.5% and pharyngeal 36.2%. The most frequent complications were mediastinitis thoracic dissemination 24.6%, sepsis 21.7% and spontaneous fistula 20.3% with orostome or pharyngostome. Medico-surgical treatment was associated with a reanimation in most cases. The outcome was favorable in 79.7% of cases. Mortality was 17.4%. Conclusion: The complications of cervicofacial cellulitis are frequent and often life-threatening. Their management is done in a multidisciplinary framework. The prevention and early treatment remain the pledge of their control.

关键词： Complications Cervicofacials Cellulitis

Management of Vulnating Esophageal Foreign Bodies in Burkina Faso

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