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Accuracy comparison of dry matter intake prediction models evaluated by a feeding trial of lactating dairy cows fed two total mixed rations with different forage source

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Journal of Integrative Agriculture 2017年第4期16卷 921-929页

作者： PAN Xiao-hua YANG Liang yves beckers XIONG Ben-hai JIANG Lin-shu State Key Laboratory of Animal Nutrition Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193P.R.China University of Liege Gembloux Agro-Bio TechPrecision Livestock and NutritionB-5030 GemblouxBelgium Beijing Key Laboratory for Dairy Cow Nutrition Beijing University of AgricultureBeijing 102206P.R.China

Dry matter intake （DMI） prediction models of NRC （2001）, Fox et aL （2004） and Fuentes-Pila et aL （2003） were targeted in the present study, and the objective was to evaluate their prediction accuracy with feeding trial data of 32 lactating Holstein cows fed two total mixed rations with different forage source. Thirty-two cows were randomly assigned to one of two total mixed ration groups： a ration containing a mixed forage （MF） of 3.7% Chinese wildrye, 28.4% alfalfa hay and 26.5% corn silage diet and another ration containing 33.8% corn stover （CS） as unique forage source. The actual DMI was greater in MF group than in CS group （P=0.064）. The NRC model to predict DMI resulted in the lowest root mean square prediction error for both MF and CS groups （1.09 kg d-1 vs. 1.28 kg d-1） and the highest accuracy and precision based on concordance correlation coefficient for both MF and CS diet （0.89 vs. 0.87）. Except the NRC model, the other two models presented mean and linear biases in both MF and CS diets when prediction residuals were plotted against predicted DMI values （P〈0.001）. The DMI variation in MF was caused by week of lactation （55.6%）, milk yield （13.9%）, milk fat percentage （7.1%） and dietary neutral detergent fiber （13.3%）, while the variation in CS was caused by week of lactation （50.9%）, live body weight （28.2%）, milk yield （8.4%）, milk fat percentage （5.2%） and dietary neutral detergent fibre （3.8%）. In a brief, the NRC model to predict DMI is comparatively acceptable for lactating dairy cows fed two total mixed rations with different forage source.

关键词： dairy cows dry matter intake model comparison mixed forage corn stover

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Diversity shifts of rumen bacteria induced by dietary forages in dairy cows and quantification of the changed bacteria using a new primer design strategy

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Journal of Integrative Agriculture 2016年第11期15卷 2596-2603页

作者： JIN Di ZHAO Sheng-guo ZHANG Yang-dong SUN Peng BU Deng-pan yves beckers WANG Jia-qi State Key Laboratory of Animal Nutrition Institute of Animal ScienceChinese Academy of Agricultural Sciences Animal Science Unit Gembloux Agro-Bio TechUniversity of Liège

The partial 16 S r RNA gene sequences（100 to 500 bp） were widely used to reveal rumen bacterial composition influenced by diets, while quantification of the changed uncultured bacteria was inconvenient due to difficult designing of specific primers based on short sequences. This study evaluated the effect of forage resources on rumen bacterial diversity and developed new strategy for primer design based on short sequences to quantify the changed uncultured bacteria. Denaturing gradient gel electrophoresis（DGGE） analysis and subsequent band sequencing were used to reveal the distinct rumen bacteria composition in cows fed with two forage sources（single corn stover vs. mixed forages including alfalfa hay and corn silage）. The bacterial diversity in the rumen of dairy cows fed with corn stover was lower than that with mixed forages（P0.05）. The bacterium named R-UB affiliating to uncultured Succinivibrionaceae was identified, and it was abundant in the rumen of cows fed with mixed forages compared to corn stover. The full length 16 S r RNA gene sequences with identity of 97% to the R-UB 16 S r RNA gene sequence were obtained from Gen Bank and used to design specific primers to quantify uncultured bacterium R-UB. All sequences of amplicon from the new primers were of 100% identity to R-UB sequences indicating the high specificity of new primers. Quantitative PCR confirmed that abundance of R-UB in the rumen of cows fed with corn stover was lower than those fed with mixed forages（P0.01）. New strategy for designing primers based on partial 16 S r RNA genes to quantify targeted uncultured bacteria was successfully developed. The rumen bacteria descending significantly in the cows fed corn stover compared to those fed mixed forages was identified as uncultured R-UB from Succinivibrionaceae.

关键词： rumen bacterial diversity forage source primers qPCR

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Approximate Compositional Values and Tissue Fatty Acid Profiles of Nile Tilapia (<i>Oreochromis niloticus</i>L.) Fed <i>Azolla</i>-Diets in Earthen Ponds

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Food and Nutrition Sciences 2011年第9期2卷 964-973页

作者： Youssouf Abou Emile Didier Fiogbé yves beckers Jean-Claude Micha 不详

The approximate general composition and the fatty acid profile of Nile tilapia fed Azolla-diets in ponds were studied for 90 days. Six isonitrogenous (29.2% CP) and isoenergetic (16.9 kJ·g>sup>-1) diets were formulated to contain 0% (A0), 10% (A10), 20% (A>sub>20), 30% (A30), 40% (A40) and 50% (A50) of Azolla meal (AM), as partial fish meal (FM) substitutes. Diet A0 without AM served as a control. Fish growth decreased as AM level exceeded 20% in diets (P 0.05). Crude lipid was significantly lower in fish fed A50 and significant differences were also found in crude ash (P 20, A30 and A50. High level of the fern reduces growth without negatively affecting fatty acid in fish. Fish PUFA, especially the (n - 3) fatty acids, are affected positively, even when fed 50% AM, which is good for the quality of the fish produced in regard to the benefits for the health of consumers.

关键词： Azolla Filiculoides Fish Meal Replacement Oreochromis Niloticus Fatty Acid Natural Food Earthen Pond

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