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Gene cloning and expression of cadherin in midgut of Helicoverpa armigera and its Cry1A binding region

Science China(Life Sciences) 2005年第4期48卷 346-356页

作者： WANG Guirong WU Kongming LIANG Gemei guo yuyuan State Key Laboratory of Plant Disease and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100094China

Cadherins belong to one of the families of animal glycoproteins responsible for cal-cium-dependent cell-cell *** literatures showed that the cadherin-like in midgut of several insects served as the receptor of Bt toxin Cry1A and the variation of cadherin-like is re-lated to insect’s resistance to *** full-length cDNA encoding cadherin-like of Helicoverpa armigera is cloned by degenerate PCR and RACE techniques and the gene was designated as BtR-harm,which is 5581 bp in full-length,encoding 1730 amino acid residues(BtR-harm was deposited in GenBank and the accession number is AF519180).Its predicted molecular weight and isoelectric point were 195.39 kDa and 4.23,*** inferred amino acid sequence includes a signal sequence,11 cadherin repeats,a membrane-proximal region,a transmem-brane region and a cytoplasmic *** analysis indicated that the deduced protein sequence was most similar to the cadherin-like from Heliothis virescens with 84.2%identity and highly similar to three other lepidopteran cadherin from Bombyx mori,Manduca sexta and Pectinophora gossypiella,with the sequence identities of 60.3.6%,57.5%and 51.0%,*** cDNA encoding cadherin gene was expressed successfully in *** and the recom-binant proteins can bind with *** analysis and binding experiment of BtR-harm revealed that the Cry1A binding region was a contiguous 244-amino acid sequence,which lo-cated between amino acid 1217 and ***-quantitative RT-PCR analysis showed that BtR-harm was highly expressed in midgut of ***,very low expressed in foregut and hindgut and was not expressed in other *** *** producing resistance to Cry1Ac,the expression quantity of BtR-harm significantly decreased in midgut of *** is the first confirmation that BtR-harm can function as receptor of Cry1Ac in *** and the binding region was located on a contiguous 244 amino acid sequence,suggesting that the de-crease o

关键词： BtR-harm Bt receptor gene cloning and expression Helicoverpa armigera.

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Cytotoxicity and binding profiles of activated CrylAc and Cry2Ab to three insect cell lines

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Insect Science 2018年第4期25卷 655-666页

作者： Jizhen Wei Gemei Liang Kongming Wu Shaohua Gu yuyuan guo Xinzhi Ni Xianchun Li State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing China Department of Entomology and BIO5 Institute University of Arizona Tucson AZ USA USDA-ARS Crop Genetics and Breeding Research Unit Tifton GA USA

While CrylAc has been known to bind with larval midgut proteins cad- herin, APN （amino peptidase N）, ALP （alkaline phosphatase） and ABCC2 （adenosine triphosphate-binding cassette transporter subfamily C2）, little is known about the recep- tors of Cry2Ab. To provide a clue to the receptors of Cry2Ab, we tested the baseline cytotoxicity of activated Cry 1Ac and Cry2Ab against the midgut and fat body cell lines of Helicoverpa zea and the ovary cell line ofSpodopterafrugiperda （SFg）. As expected, the descending order of cytotoxicity of CrylAc against the three cell lines in terms of 50% lethal concetration （LC50） was midgut （31.0μg/mL）〉 fat body （59.0μg/mL） and SF9 cell （99.6μg/mL）. By contrast, the fat body cell line （LC50 = 7.55μg/mL） was about twice more susceptible to Cry2Ab than the midgut cell line （16.0/xg/mL）, the susceptibility of which was not significantly greater than that of SF9 cells （27.0μg/mL）. Further, ligand blot showed the binding differences between CrylAc and Cry2Ab in the three cell lines. These results indicated that the receptors of Cry2Ab were enriched in fat body cells and thus largely different from the receptors of CrylAc, which were enriched in midgut cells.

关键词： Bt cell toxicity receptors midgut cell fat body cell SF9 cell

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Cloning,expression and binding specificity analysis of odorant binding protein 3 of the lucerne plant bug,Adelphocoris lineolatus(Goeze)

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Chinese Science Bulletin 2010年第34期55卷 3911-3921页

作者： GU ShaoHua SUN Yang REN LiYan ZHANG XueYing ZHANG YongJun WU KongMing guo yuyuan State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural SciencesBeijing 100193 China College of Agriculture and Biotechnology China Agricultural University Beijing 100193 China

Credible evidence shows that odorant binding proteins(OBPs)are required for insect olfaction perception and play a key role in transporting hydrophobic odorants across the sensillum lymph to the olfactory receptors(ORs).In the present study,a novel OBP(AlinOBP3)gene from the lucerne plant bug,Adelphocoris lineolatus,was cloned and *** expression pattern of AlinOBP3 was evaluated by qPCR,which indicated that AlinOBP3 was dominantly expressed in *** binding properties of AlinOBP3 with 9 cotton volatiles and 5 sex pheromone analogs were measured by fluorescence competitive binding assays with the fluorescence probe *** results revealed that of 9 cotton volatiles,Myrcene,β-Ocimene and α-Phellandrene can bind with AlinOBP3.α-Phellandrene especially bound to AlinOBP3 with a high binding affinity,with a dissociation constant of 56.68 μmol/*** the 5 sex pheromone analogs,Hexyl butyrate had the strongest binding affinity with AlinOBP3,with a dissociation constant as 59.53 μmol/*** butyrate,trans-2-Hexenyl butyrate and Ethyl butyrate had medium binding affinities with AlinOBP3,with dissociation constants of 227.39,108.77 and 143.47 μmol/L,*** results suggest that AlinOBP3 might be a pheromone binding protein(PBP)with a dual-function for the perception of sex pheromones and plant volatiles.

关键词：气味结合蛋白克隆和表达结合特异性线纹结合亲和力植物挥发物性信息素解离常数

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The recent disciplinal progresses of agricultural entomology in China

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Engineering Sciences 2009年第3期7卷 2-15页

作者： guo yuyuan Liang Gemei State Key Laboratory of Plant Disease and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100094 China

In this paper, four recent advances and achievements of China in agricultural insect research, namely, on the genome of silkworm (Bombyx mori Linnaeus), on the geographical differentiation and regional migration of cotton bollworm (Helicoverpa armigera (Hübner)), on the standardized monitoring techniques for safety of honey bee (Apis mellifera Linnaeus) products, and on the virus transmission property of small brown planthopper (Laodelphax striatellus (Fallén)) as well as the interactions between vector and rice stripe virus (RSV), were reported. All of these researches are very important for controlling agricultural insect pests and the diseases they transmit, accelerating the molecular biological research of silkworm, and promoting the international trade of honey bee products. Most of these achievements mentioned above have got the national, provincial, ministerial or municipal awards on science and technology.

关键词： recent advances and achievements of China agricultural insect research control agricultural insect pests and diseases molecular biology research international trade

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Illustrations with Real Examples of Using Ecological Regulation Strategies Against Crop Pests in China

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工程科学（英文版） 2006年第4期4卷 8-18页

作者： guo yuyuan Liang Gemei State Key Laboratory of Plant Disease and Inset Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences Beijing 100094

In this paper, a series of examples from China, including those using ecological regulation strategies successfully to keep crop pests under control and those making more damages of crop pests caused by wrong cropping systems, crop distributions or extensive cultivation, were given and analyzed. From these, two pieces of experience were enlightened as follows: the first, the reforms of cropping systems and crop distributions must be beneficial to pest control or at least not increase the damage of crop pests; the second, we should further clarify the relationship between population dynamics of pests and cropping system/crop distribution, the basis for correctly laying down the ecological regulation systems.

关键词：生态环境生态调节植物植被

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我国农作物病虫害生态调控实例分析

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植物保护 2006年第2期32卷 1-4页

作者：郭予元中国农业科学院植物保护研究所北京100094

从改造病虫害发生区的生态环境、合理调整作物布局、实施合理种植制度、加强栽培管理几个方面,用已往通过生态调控措施成功地控制病虫害以及因不合理改制或栽培管理粗放造成病虫害大发生的典型例子进行分析。由此得出两点体会:1.在推行... 详细信息

从改造病虫害发生区的生态环境、合理调整作物布局、实施合理种植制度、加强栽培管理几个方面,用已往通过生态调控措施成功地控制病虫害以及因不合理改制或栽培管理粗放造成病虫害大发生的典型例子进行分析。由此得出两点体会:1.在推行栽培制度、作物布局改革和作物品种更替时,一定要考虑其对农作物病虫害发生动态的影响,以有利于控制病虫害或至少不加重病虫害的发生危害为基本原则。2.需要进一步分析病虫害种群动态受作物布局及耕作制度制约的机理,由此制定合理的生态调控措施。

关键词：农作物病虫害改造生态环境调整作物布局合理种植制度生态调控

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Impact of the Belt and Road Initiative:The case of Pakistan

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Economic and Political Studies 2024年第3期12卷 235-249页

作者： Zhanming Chen Yasir Amin Ruiping Jiang Shan guo yuyuan Wen Xinye Zheng School of Applied Economics Renmin University of ChinaBeijingChina School of Public Administration and Policy Renmin University of ChinaBeijingChina

This study investigates the macroeconomic impact of the Belt and Road Initiative(BRI)on Pakistan using the synthetic control *** on the panel data of 42 non-BRI countries,we construct a synthetic Pakistan to estimate the economic growth trend of the country without the *** comparing the actual economy with the synthetic case,our results show that the BRI increases the annual per capita GDP growth rate of Pakistan from 3.04%to 4.69%for the period ***’s per capita GDP in 2018 would have been Int$5,022 instead of Int$5,567 if the BRI had not *** outcomes of the in-time placebo test,in-place placebo test,and dropping sample test all confirm the robust impact of the BRI on Pakistan.

关键词： Belt and Road Initiative economic growth Pakistan synthetic control method

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棉铃虫种群的地理型分化和区域性迁飞规律

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植物保护 2007年第5期33卷 6-11页

作者：吴孔明郭予元中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室北京100094

对棉铃虫地理种群的遗传变异、滞育性状和抗寒性分化等的系统研究表明,我国棉铃虫由热带型、亚热带型、温带型和新疆型4个地理型组成,各地理型对华南地区、长江流域地区、黄河流域地区和新疆地区的气候环境具有高度专化适应性。受东亚... 详细信息

对棉铃虫地理种群的遗传变异、滞育性状和抗寒性分化等的系统研究表明,我国棉铃虫由热带型、亚热带型、温带型和新疆型4个地理型组成,各地理型对华南地区、长江流域地区、黄河流域地区和新疆地区的气候环境具有高度专化适应性。受东亚季风影响,5～7月份温带型1代和2代棉铃虫成虫随偏南风向北迁移,可迁入山西北部、河北北部、辽宁等非越冬区的中温带地区。温带型3代和4代棉铃虫于8月中旬以后,随偏北风向南回迁。成虫种群密度过大和所处的不良环境是引起迁飞的主要原因。

关键词：棉铃虫地理型迁飞

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Integrative analysis of tumor stemness and immune microenvironment deciphers novel molecular subtypes in hepatocellular carcinoma

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Genes & Diseases 2024年第5期11卷 81-84页

作者： Zhiyi Wang Shuang Chen Dongxiao Li Hui Xu Siyuan Weng yuyuan Zhang Yuqing Ren Chunguang guo Xiuling Li Zaoqu Liu Xinwei Han Department of Interventional Radiology The First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenan 450052China Department of Gastroenterology Henan Provincial People’s HospitalZhengzhou University People’s HospitalZhengzhouHenan 450004China Center of Reproductive Medicine The First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenan 450052China Department of Respiratory and Critical Care Medicine The First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenan 450052China Department of Endovascular Surgery The First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenan 450052China State Key Laboratory of Proteomics Beijing Proteome Research CenterNational Center for Protein Sciences(Beijing)Beijing Institute of LifeomicsBeijing 102206China State Key Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical SciencesDepartment of PathophysiologyPeking Union Medical CollegeBeijing 100730China

Hepatocellular carcinoma(HCC)is a highly heterogeneous tumor,with dynamic equilibrium and complex interplay between its intricate tumor nature and ambient tumor immune microenvironment(TIME).1 Elegant research has indicated that cancer stem cells,a small subset of neoplastic cells confined within dedicated niches,display stem cell-like properties and interact with cells in TIME,thereby imparting an indelible impact on stemness regulation,tumor heterogeneity,and cancer cell plasticity.2 Previous taxonomies solely from the perspective of stemness or TIME may introduce some degree of bias in the comprehension of HCC carcinogenesis,3,4 and thus it is of paramount importance to systematically consider tumor stemness and TIME as a whole to truly portray the biological landscape of HCC.

关键词： thereby landscape stem

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棉铃虫对Bt的抗性筛选及交互抗性研究

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中国农业科学 2000年第4期33卷 46-53页

作者：梁革梅谭维嘉郭予元中国农业科学院植物保护研究所!北京100094

通过室内筛选获得了棉铃虫 H elicoverpa armigera ( Hübner)对 Bt杀虫剂、Bt毒蛋白和转 Bt基因棉的抗性种群 ,并在此基础上测定了与其它 Bt、化学杀虫剂、新鲜转 Bt基因棉的交互抗性。结果表明 ,经 1 6代筛选 ,棉铃虫对 3种 Bt都可以... 详细信息

通过室内筛选获得了棉铃虫 H elicoverpa armigera ( Hübner)对 Bt杀虫剂、Bt毒蛋白和转 Bt基因棉的抗性种群 ,并在此基础上测定了与其它 Bt、化学杀虫剂、新鲜转 Bt基因棉的交互抗性。结果表明 ,经 1 6代筛选 ,棉铃虫对 3种 Bt都可以产生抗性 ,抗性上升的快慢顺序是 :Bt杀虫剂 >Bt毒蛋白 >转 Bt基因棉。筛选过程中 ,棉铃虫 7日龄幼虫的体重和成虫羽化率变化明显 ,被选为确定筛选剂量的标准。棉铃虫对 Bt产生抗性后 ,与含有相同基因的其它 Bt产品存在正交互抗性 ,与含有不同基因型的 Bt有较低的交互抗性或负交互抗性。用田间新鲜棉叶测定也得到相同的结果。抗性种群的 7日龄幼虫对溴氰菊酯、氰戊菊酯、辛硫磷、硫丹的敏感性明显提高 ,但不同种群的 3龄幼虫的反应差异较大。已经对转 Bt基因棉花有抗性的棉铃虫 ,取食新鲜的转 Bt基因棉棉叶死亡率明显降低

关键词：棉铃虫 Bt 抗性交互抗性

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