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Programmable Analytical Feature of Ratiometric Electrochemical Biosensor by Alternating the Binding Site of Ferrocene to DNA Duplex for the Detection of Aflatoxin B1

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Chinese Journal of Chemistry 2022年第18期40卷 2232-2238页

作者： Dong liu Fan Jia Ya Wei yuye li Shuyun Meng Tianyan You Key Laboratory of Modern Agricultural Equipment and Technology Ministry of EducationSchool of Agricultural EngineeringJiangsu UniversityZhenjiangJiangsu212013 China

Ratiometric biosensor is among the promising strategies for the advanced analysis;its applicability in multiple scenarios,however,is seriously limited for the fixed analytical feature.Here,we reported ratiometric electrochemical biosensors with programmable analytical feature by alternating the binding site of ferrocene(Fc)to DNA duplex for the detection of aflatoxin B1(AFB1).DNA duplex was synthesized with AFB1 aptamer and its complementary DNA(cDNA)with Fc molecular tagged on aptamer or cDNA.For analysis,aptamer-AFB1 complex stripped from the electrode,and free cDNA triggered hybridization chain reaction to produce double-stranded DNA for methylene blue(MB)absorption.In this way,redox currents of Fc(I_(Fc))and absorbed MB(I_(MB))at the electrode were collected to produce the ratiometric signal of I_(MB)/I_(Fc)as a yardstick for AFB1.When Fc was tagged on aptamer,IFc decreased with higher concentration of AFB1(C_(AFB1)),the resultant biosensor allowed detection with an On/Off(I_(MB)/I_(Fc))mode;when Fc was tagged on cDNA,I_(Fc) kept constant as C_(AFB1)varying,offering an On/Constant(I_(MB)/I_(Fc))mode for the detection.Consequently,biosensor with On/Off mode showed a linear range of 0.1—100 pg/mL with a limit of detection(LOD)of 0.0650 pg/mL,while biosensor with On/Constant mode offered a wider linear rang of 1—10000 pg/mL but a relatively poor LOD of 0.330 pg/mL.Our work revealed the vital importance of binding site of probes to DNA,and this principle can endow ratiometric biosensor with programmable analytical feature(e.g.,linear range,LOD),allowing detections in various scenarios.

关键词： Electrochemistry Biosensors Aptamers Ratiometric detection Aflatoxins

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Epidemiology of urticaria in China:a population-based study

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Chinese Medical Journal 2022年第11期135卷 1369-1375页

作者： Jiaqing li Dandan Mao Shuoshuo liu Ping liu Jing Tian Chenhong Xue Xiaojing liu Ruiqun Qi Bingxue Bai Jianjun Nie Siqi Ye Yu Wang yuye li Qing Sun Juan Tao Shuping Guo Hong Fang Jianqin Wang Qiri Mu Quanzhong liu Yan Ding Jianzhong Zhang Department of Dermatology Peking University People’s HospitalBeijing 100044China Department of Dermatology Beijing Children’s HospitalCapital Medical UniversityBeijing 100045China Department of Dermatology Henan Provincial People’s HospitalZhengzhouHenan 450003China Optical Medicine Institute of Tongji University in Shanghai Skin Disease Hospital Shanghai 200071China Department of Dermatology The First Hospital of China Medical UniversityShenyangLiaoning 110001China Department of Dermatology The Second Affiliated Hospital of Harbin Medical UniversityHarbinHeilongjiang 150001China Department of Dermatology Chengdu Second People’s HospitalChengduSichuan 610017China Department of Dermatology The Second Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhouGuangdong 510120China Department of Dermatology Affiliated Hospital of Guizhou Medical UniversityGuiyangGuizhou 550004China Department of Dermatology The First Affiliated Hospital of Kunming Medical UniversityKunmingYunnan 650031China Department of Dermatology Qilu Hospital of Shandong UniversityJinanShandong 250012China Department of Dermatology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430022China Department of Dermatology First Affiliated Hospital of Shanxi Medical UniversityTaiyuanShanxi 030001China Department of Dermatology The First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhouZhejiang 310003China Department of Dermatology Guangzhou Institute of DermatologyGuangzhouGuangdong 510095China Department of Dermatology Inner Mongolia People’s HospitalHohhotInner Mongolia 010017China Department of Dermatology Tianjin Medical University General HospitalTianjin 300052China Department of Dermatology Hainan Provincial Hospital of Skin DiseaseHaikouHainan 570206China.

Background:Urticaria is a common skin disease characterized by episodes of wheals,and it has a negative effect on patients’quality of life.Large-scale population-based epidemiological studies of urticaria are scarce in China.The aim of this survey was to determine the prevalence,clinical forms,and risk factors of urticaria in the Chinese population.Methods:This survey was conducted in 35 cities from 31 provinces,autonomous regions,and municipalities of China.Two to three communities in each city were selected in this investigation.Participants completed questionnaires and received dermatological examinations.We analyzed the prevalence,clinical forms,and risk factors of urticaria.Results:In total,44,875 questionnaires were distributed and 41,041 valid questionnaires were collected(17,563 male and 23,478 female participants).The lifetime prevalence of urticaria was 7.30%,with 8.26%in female and 6.34%in male individuals(Pliving in urban areas,exposure to pollutants,an anxious or depressed psychological status,a personal and family history of allergy,thyroid diseases,and Helicobacter pylori infection were associated with a higher prevalence of urticaria.Smoking was correlated with a reduced risk of urticaria.Conclusion:This study demonstrated that the lifetime prevalence of urticaria was 7.30%and the point prevalence was 0.75%in the Chinese population;women had a higher prevalence of urticaria than men.Various factors were correlated with urticaria.

关键词： Urticaria Prevalence Risk factor China

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A Novel 2-dimensional Multiplex qPCR Assay for Single-Tube Detection of Nine Human Herpesviruses

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Virologica Sinica 2021年第4期36卷 746-754页

作者： Yingxue li Zhenzhou Wan Lulu Zuo Shenwei li Honglian liu Yingying Ma lianqun Zhou Xia Jin yuye li Chiyu Zhang Shanghai Public Health Clinical Center Fudan UniversityShanghai201508China CAS Key Laboratory of Bio-Medical Diagnostics Suzhou Institute of Biomedical Engineering and TechnologyChinese Academy of SciencesSuzhou215163China School of Biomedical Engineering University of Science and Technology of ChinaHefei260026China Medical Laboratory of Taizhou Fourth People's Hospital Taizhou225300China Viral Hemorrhagic Fevers Research Unit Institut Pasteur of ShanghaiChinese Academy of SciencesShanghai200031China Shanghai International Travel Healthcare Center Shanghai200335China Department of Dermatology and Venereology First Affiliated Hospital of Kunming Medical UniversityKunming650032China

Human herpesviruses are double-stranded DNA viruses that are classified into nine species.More than 90%of adults are ever infected with one or more herpesviruses.The symptoms of infection with different herpesviruses are diverse ranging from mild or asymptomatic infections to deadly diseases such as aggressive lymphomas and sarcomas.Timely and accurate detection of herpesvirus infection is critical for clinical management and treatment.In this study,we established a single-tube nonuple qPCR assay for detection of all nine herpesviruses using a 2-D multiplex qPCR method with a house-keeping gene as the internal control.The novel assay can detect and distinguish different herpesviruses with 30 to 300 copies per 25µL single-tube reaction,and does not cross-react with 20 other human viruses,including DNA and RNA viruses.The robustness of the novel assay was evaluated using 170 clinical samples.The novel assay showed a high consistency(100%)with the single qPCR assay for HHVs detection.The features of simple,rapid,high sensitivity,specificity,and low cost make this assay a high potential to be widely used in clinical diagnosis and patient treatment.

关键词： Human herpesviruses(HHVs) 2-D multiplex qPCR Melting temperature(Tm) Fluorescence probe Melting curve

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基于低碳理念下的居住区景观全生命周期碳平衡研究

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园林 2024年第2期41卷 111-118页

作者：李昱烨唐红甘肃农业大学林学院兰州730070

居住区景观是人们日常生活中最常接触到的城市绿地,对于实现“碳中和”目标具有至关重要的作用。城市景观不仅具备植物碳汇功能,且涵盖了景观材料的生产、建造、使用和维护周期的碳排放。以甘肃省白银市国际城小区为例,通过实地调查研... 详细信息

居住区景观是人们日常生活中最常接触到的城市绿地,对于实现“碳中和”目标具有至关重要的作用。城市景观不仅具备植物碳汇功能,且涵盖了景观材料的生产、建造、使用和维护周期的碳排放。以甘肃省白银市国际城小区为例,通过实地调查研究及参考相应标准碳排放因子,计算景观全生命周期的碳排放量,揭示出景观材料生产阶段占总碳排放量的比重最大(47.08%),其次是景观使用阶段(27.68%)。参考植物单位叶面积和单位叶面积固碳量,计算居住区植物景观全生命周期碳汇量为2 530.36 t。分析碳源和碳汇的相互转化过程可得,居住区景观在建成的第48年达到碳平衡状态。基于对居住区景观全生命周期碳平衡的分析,提出了低碳理念下居住区景观的减排和增汇策略,进而初步探讨低碳景观评价体系,为可持续发展的城市景观建设提供实践参考。

关键词：居住区景观景观全生命周期碳平衡碳排放植物碳汇

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The number and cytotoxicity and the expression of cytotoxicity-related molecules in peripheral natural killer(NK)cells do not predict the repeated implantation failure(RIF)for the in vitro fertilization patients

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Genes & Diseases 2020年第2期7卷 283-289页

作者： Hongzhan Zhang Chunyu Huang Xian Chen Longfei li Su liu yuye li Yongnu Zhang Yong Zeng lina HuDepartment of Obstetrics and Gynecology The Second Affiliated Hospital of Chongqing Medical University Chongqing400010PR China Shenzhen Key Laboratory of Reproductive Immunology for Peri-implantation Shenzhen ZhongshanInstitute for Reproduction and GeneticsFertility CenterShenzhen Zhongshan Urology HospitalShenzhenGuangdong518045PR China

Natural killer(NK)cells are thought to play a key role in the successful establishment of a pregnancy by facilitating immunological adaptation of the semi-allogeneic developing embryo.The aim of this study was to explore the cell number,immunophenotypic characteristics,and activities of peripheral blood NK cells in women with repeated implantation failure(RIF).Peripheral blood was obtained from 27 women with RIF and 11 healthy,fertile controls during the middle luteal phase of the menstrual cycle.CD3^-CD56^+NK cells were quantified and analyzed by flow cytometry for the expression of cytolytic molecules(granzyme B,granulysin,and perforin)as well as cell surface receptors responsible for NK cell activation or inhibition(NKG2D,NKp30,NKp46,CD158a,CD158b).NK cytotoxicity was measured at three effector-to-target cell ratios.Women with RIF and fertile controls did not differ significantly in the percentage of circulating CD3CD56t NK cells,or in the proportions of these cells that expressed granzyme B,granulysin,or perforin.The two groups also did not differ significantly in the proportions of NK cells expressing the receptors NKG2D,NKp30,NKp46,CD158a or CD158b.General linear model analysis showed that NK cytotoxicity increased with effector-to-target cell ratio.However,NK cytotoxicity did not differ significantly between patients with RIF and fertile controls.These results suggest that RIF is not associated with significant alterations in the number or function of peripheral blood NK cells.

关键词： Activating receptor Inhibitory receptor NK cytotoxicity Peripheral blood NK cells Repeated implantation failure

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两栖动物生物活性肽对皮肤的保护作用研究进展

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皮肤病与性病 2024年第3期46卷 157-162页

作者：黄买银李哲张单李玉叶昆明医科大学第一附属医院皮肤科云南昆明650032

皮肤是人体最大的器官,具有多种生理功能,对人体健康至关重要。然而,皮肤经常面临外部环境压力,常常导致创伤、感染、炎症和肿瘤等问题。近年来,两栖动物生物活性肽因具有抗氧化、促进伤口愈合、抗感染、抗炎和抗癌等作用,在治疗皮肤损... 详细信息

皮肤是人体最大的器官,具有多种生理功能,对人体健康至关重要。然而,皮肤经常面临外部环境压力,常常导致创伤、感染、炎症和肿瘤等问题。近年来,两栖动物生物活性肽因具有抗氧化、促进伤口愈合、抗感染、抗炎和抗癌等作用,在治疗皮肤损伤中展现出巨大的研究和应用潜力,是开发皮肤科新药的宝库。本文综述了两栖动物生物活性肽对皮肤的保护作用及其未来应用的可能性,为两栖动物生物活性肽在治疗皮肤损伤方面的研究提供参考。

关键词：两栖动物生物活性肽皮肤保护抗氧化伤口愈合

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宿主免疫与HIV 相关隐球菌性脑膜炎预后研究进展

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皮肤病与性病 2024年第3期46卷 168-171页

作者：赵树伟苏瑞李钰春董天祥李玉叶李红宾昆明医科大学第一附属医院皮肤科云南昆明650032 昆明医科大学海源学院云南昆明650106

即使在全球开展抗逆转录病毒治疗和抗真菌药物治疗背景下,HIV相关隐球菌性脑膜炎仍然是艾滋病患者死亡的重要原因。宿主免疫状态是决定HIV相关隐球菌性脑膜炎预后的关键因素,近年来宿主免疫与HIV相关隐球菌性脑膜炎预后相关性的研究取... 详细信息

即使在全球开展抗逆转录病毒治疗和抗真菌药物治疗背景下,HIV相关隐球菌性脑膜炎仍然是艾滋病患者死亡的重要原因。宿主免疫状态是决定HIV相关隐球菌性脑膜炎预后的关键因素,近年来宿主免疫与HIV相关隐球菌性脑膜炎预后相关性的研究取得一些进展,本文就宿主先天免疫、适应性免疫、中枢神经系统免疫及免疫重建综合征对HIV相关隐球菌性脑膜炎预后的影响进行综述。

关键词： HIV相关隐球菌性脑膜炎先天免疫适应性免疫中枢神经系统免疫免疫重建综合征

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LaMnO_(3)/Co_(3)O_(4) nanocomposite for enhanced triethylamine sensing properties

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Journal of Rare Earths 2024年第4期42卷 733-742页

作者： Jian Zhang Yanping Chen ling li Xue Chen Wenya An Xiujuan Qian yuye Tao School of Science Shandong Jianzhu UniversityJinan250100China

LaMnO_(3) modified Co_(3)O_(4) nanocomposites were prepared by simple hydrothermal method co mbined with sol-gel method.The gas sensitivity properties of pure Co_(3)O_(4) and LaMnO_(3)/Co_(3)O_(4) with different composite proportions are compared.It is found that 0.6-LMO/Co_(3)O_(4) sensor has higher sensitivity to triethylamine(TEA)than pure Co_(3)O_(4) sensor,which is improved by 9.27 times.And the working temperature is reduced from 150 to 130℃.Besides,it has excellent gas selectivity and repeatability.The improvement of the gas sensitivity of LaMnO_(3)/Co_(3)O_(4) sensor may be due to the fact that LaMnO_(3) is an effective catalyst,and the catalytic performance perhaps is beneficial to improving the sensing performance.In addition,the formation of p-p heterojunctions may be the key factor to improve the gas sensing performance.This work provides a new Co_(3)O_(4)-based gas sensing material for the detection of TEA.

关键词： Gas sensing Co_(3)O_(4) LaMnO_(3) Triethylamine Rareearths

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The nonlinear mechanism for the same responses of neuronal bursting to opposite self-feedback modulations of autapse

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Science China(Technological Sciences) 2021年第7期64卷 1459-1471页

作者： li yuye GU HuaGuang JIA Bing DING Xueli College of Mathematics and Computer Science Chifeng UniversityChifeng024000China School of Aerospace Engineering and Applied Mechanics Tongji UniversityShanghai200092China Department of Physiology and Biophysics School of Life SciencesFudan UniversityShanghai200438China Department of Basic Education Fuyang Institute of TechnologyFuyang236031China

In the traditional viewpoint,inhibitory and excitatory effects always induce opposite responses.In the present study,the enhanced bursting activities induced by excitatory autapses,which are consistent with the recent experimental observations,and those induced by inhibitory autapses,which is a paradoxical phenomenon,were simulated using the Chay model.The same bifurcations and different ionic currents for the same responses were acquired with fast-slow variable dissection and current decomposition,respectively.As the inhibitory or excitatory autaptic conductance increased,the ending phase of the burst related to a homoclinic bifurcation of the fast subsystem changed to widen the burst duration to contain more spikes,which was induced by an elevated minimal potential(V_(min)) of spiking of the fast subsystem.Larger inhibitory and excitatory autaptic conductances induced smaller and larger maximal potentials(V_(max)) of spiking,respectively.During the downstroke,a weaker potassium current induced by the smaller V_(max) played a dominant role for the inhibitory autapse,and the stronger potassium current induced by the larger V_(max) became weaker due to the opposite autaptic current of the excitatory autapse,which induced the V_(min) elevated.The results present the nonlinear and biophysical mechanisms of the same responses to opposite effects,which extends nonlinear dynamics knowledge and provides potential modulation measures for the nervous system.

关键词： bifurcation bursting autapse inhibitory effect excitatory effect fast-slow variable dissection

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PDCA循环在缩短性激素样本周转时间中的应用

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临床检验杂志 2024年第4期42卷 308-311页

作者：杨小兵庄佩玲廖婉婷李玉叶吴彤华深圳中山妇产医院检验中心广东深圳518016

目的运用PDCA循环优化管理,缩短性激素5项检测的样本周转时间(TAT),提高临床和患者满意度。方法成立PDCA管理小组,回顾分析2021年性激素TAT现状。2022年应用PDCA工具,绘制鱼骨图分析原因、柏拉图确定真因,制定并实施相应改进措施,监测P... 详细信息

目的运用PDCA循环优化管理,缩短性激素5项检测的样本周转时间(TAT),提高临床和患者满意度。方法成立PDCA管理小组,回顾分析2021年性激素TAT现状。2022年应用PDCA工具,绘制鱼骨图分析原因、柏拉图确定真因,制定并实施相应改进措施,监测PDCA前后TAT变化。结果通过人员培训、增加检测设备、优化流程等整改措施,2022年(PDCA改善后)性激素TAT中位数为54 min,较2021年(改善前)的61 min显著下降(P<0.05)。结论PDCA循环管理有效缩短了性激素标本TAT,提高了临床诊疗效率和患者就医体验,改善了医患关系,同时也提升了实验室质量管理水平。

关键词： PDCA循环性激素样本周转时间

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