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Alterations in expression and localization of POMGNT1 in the APP/PS1 mouse model of Alzheimer’s disease

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Genes & Diseases 2024年第5期11卷 55-58页

作者： Hanxiao jiang yuxue Feng Xia Hao Guiqiong He Xiaofeng Li Department of Neurology The Second Affiliated Hospital of Chongqing Medical UniversityChongqing 401336China Chongqing Key Laboratory of Neurobiology Chongqing Medical UniversityChongqing 400016China Department of Anatomy Chongqing Medical UniversityChongqing 400016China Department of Neurology The Fifth People’s Hospital of ChongqingChongqing 400062China

Alzheimer’s disease(AD)is a complex and progressive neurodegenerative disorder that causes dementia in the aging *** of the characteristic pathologic hallmarks of AD is the senile plaques,composed of the accumulation of *** b-amyloid results from the cleavage of transmembrane b-amyloid precursor protein(APP)by b-secretases and g-secretases,sequentially.O-mannosylation was found essential for the normal function of APP in Saccharomyces cerevisiae.1 Protein Olinked mannose b1,2-N-acetyl-glucosaminyltransferase 1(POMGNT1)is a glycosyltransferase crucial for the elongation of O-mannosyl glycans and catalyzes the transfer of Nacetylglucosamine from uridine 50-diphosphate-N-acetylglucosamine to O-mannose of glycoproteins.

关键词： APP/PS1 Alzheimer amyloid

IL-33 drives the antitumor effects of dendritic cells via the induction of Tc9 cells

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Cellular & Molecular Immunology 2019年第7期16卷 644-651页

作者： Ning Liu yuxue jiang Jintong Chen He Nan Yinghua Zhao Xiao Chu Alison Wang Dongjiao Wang Tianxue Qin Sujun Gao Ying Yue Siqing Wang Department of Gynecological Oncology The First Hospital of Jilin UniversityChangchun 130061China Department of Cancer Immunology The First Hospital of Jilin UniversityChangchun 130061China Department of Rheumatology and Immunology China-Japan Union Hospital of Jilin UniversityChangchun 130033China Department of Hematology The First Hospital of Jilin UniversityChangchun 130061China and 5 Department of Cancer BiologyLerner Research InstituteCleveland ClinicClevelandOH 44195USA

Dendritic cell(DC)tumor vaccines exert their antitumor effects through the induction of effector T *** recently identified Tc9 cells as a new potent antitumor effector T cell ***,approaches to direct DCs to preferably prime antitumor Tc9 cells should be further ***,we demonstrate that the addition of interleukin(IL)-33 potently promotes the induction of Tc9 cells by DCs in vitro and in ***-33 treatment also drives the cytotoxic activities of DC-induced Tc9 ***,IL-33 treatment enhances cell survival and proliferation of DC-primed CD8+T *** importantly,the addition of IL-33 during in vitro priming of tumor-specific Tc9 cells by DCs increases the antitumor capability of Tc9 *** studies demonstrated that IL-33 treatment inhibits exhaustive CD8+T cell differentiation by inhibiting PD-1 and 2B4 expression and increasing IL-2 and CD127(IL-7 receptor-α,IL-7Rα)expression in CD8+T ***,the addition of IL-33 further promotes the therapeutic efficacy of DC-based tumor vaccines in the OT-I mouse *** study demonstrates the important role of IL-33 in DC-induced Tc9 cell differentiation and antitumor immunity and may have important clinical implications.

关键词： Interleukin-33 Dendritic cells Tc9 Cancer immunology

Metabolomic Analysis of the Anthocyanins Associated with Different Colors of Cymbidium goeringii in Guizhou, China

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Phyton-International Journal of Experimental Botany 2024年第7期93卷 1455-1466页

作者： Dongbo Wu Shuang Qu Lianwen Shen Shengqun Chen Xia jiang Ai Rao yuxue Zhao Yueyun Wang College of Pharmacy Guizhou University of Traditional Chines MedicineGuiyang550025China Research Institute of Juglans Regia Guizhou Academy of ForestryGuiyang550005China Guizhou Liping Rocky Desertification Ecosystem National Observation and Research Station Qiandongnan Prefecture556200China

Cymbidium goeringii is an economically important ornamental plant,and flower color is one of the main features of *** that contributes to its high economic *** clarify the molecular mechanisms underlying the role of anthocyanins in mediating differences in color among varieties,liquid chromatography–tandem mass spectrometry was used to perform anthocyanin-targeted metabolomics of seven *** varieties,including‘Jin Qian Yuan’(JQY),‘Jin Xiu Qian Yuan’(JXQY),‘Miao jiang Su Die’(MJSD),‘Qian Ming Su’(QMS),‘Shi Chan’(SC),and‘Yang Ming Su’(YMS),as well as the *** detected 64 anthocyanins,including cyanidins,delphinidins,malvidins,pelargonidins,peonidins,petunidins,procyanidins,and *** identified six shared differentially accumulated metabolites(DAMs),including cyanidin-3-O-rutinoside,delphinidin-3-Osophoroside,pelargonidin-3-O-rutinoside,peonidin-3-O-(6-O-malonyl-beta-D-glucoside),peonidin-3-Osophoroside,and *** DAMs were enriched in the anthocyanin biosynthesis *** Encyclopedia of Genes and Genomes pathway analysis revealed that the differentially expressed metabolites were significantly enriched in the anthocyanin biosynthesis *** of the content of differentially expressed metabolites indicated that peonidin-3-O-(6-O-malonyl-beta-D-glucoside)was the key metabolite underlying color differences among *** *** B2,pelargonidin-3-O-galactoside,and naringenin might also affect the color formation of JQY and QMS,SC,and MJSD,*** results of this study shed light on the metabolic mechanism underlying flower color differences in *** at the molecular *** findings will aid future studies of the mechanism of flower color regulation in *** and have implications for the breeding of new varieties.

关键词： Cymbidium goeringii ﬂower color anthocyanins metabolomics

彩色多普勒超声在甲状腺肿瘤诊断中的应用研究

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中国卫生标准管理 2015年第3期6卷 54-55页

作者：姜玉学黑龙江省黑河市逊克县中医医院 164499

目的研究彩色多普勒超声在甲状腺肿瘤诊断中的应用价值。方法搜集2013年6月-2014年6月我院接收的甲状腺肿瘤59例患者,对全部患者给予彩色多普勒超声检查,并将检查结果与术后病理诊断相比较。结果彩色多普勒超声共检出7例恶性肿瘤,52例... 详细信息

目的研究彩色多普勒超声在甲状腺肿瘤诊断中的应用价值。方法搜集2013年6月-2014年6月我院接收的甲状腺肿瘤59例患者,对全部患者给予彩色多普勒超声检查,并将检查结果与术后病理诊断相比较。结果彩色多普勒超声共检出7例恶性肿瘤,52例良性肿瘤,与术后病理的诊断准确率是86.97%,结果有统计学意义（P〈0.05）。结论彩色多普勒超声对甲状腺肿瘤的诊断价值较大,诊断准确率较高。

关键词：甲状腺肿瘤彩色多普勒超声诊断应用

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38例前置胎盘的超声诊断体会

中国卫生标准管理 2014年第24期5卷 45-46页

作者：姜玉学逊克县中医医院黑龙江黑河164499

目的探讨前置胎盘的超声影像诊断价值。方法选取38例前置胎盘患者超声诊断资料进行分析。结果超声诊断为前置胎盘的38例患者足月后均以剖宫产终止妊娠。完全性前置胎盘患者11例,部分性前置胎盘患者27例,均有不同程度阴道出血。结论B型... 详细信息

目的探讨前置胎盘的超声影像诊断价值。方法选取38例前置胎盘患者超声诊断资料进行分析。结果超声诊断为前置胎盘的38例患者足月后均以剖宫产终止妊娠。完全性前置胎盘患者11例,部分性前置胎盘患者27例,均有不同程度阴道出血。结论B型超声诊断前置胎盘时须注意妊娠周数,妊娠中期胎盘占据宫腔一半的面积。对前置胎盘诊断准确率达90%-95%,经腹、阴道及直肠均可。

关键词：前置胎盘超声诊断影像表现

紫花苜蓿红色茎秆花青素苷含量及相关合成酶活性的研究

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中国草地学报 2024年第4期46卷 62-69页

作者：宗亚倩段玉雪匡倩何承刚姜华云南农业大学动物科学技术学院云南昆明650201

为研究空间诱变紫花苜蓿茎秆呈红色的原因,以空间诱变的紫花苜蓿红色茎秆和未诱变的紫花苜蓿绿色茎秆(CK)为材料,采用超高效液相色谱串联质谱(UPLC-MS/MS)技术测定茎秆中花青素苷成分和含量,用紫外可见分光光度计和ELISA试剂盒进行酶活... 详细信息

为研究空间诱变紫花苜蓿茎秆呈红色的原因,以空间诱变的紫花苜蓿红色茎秆和未诱变的紫花苜蓿绿色茎秆(CK)为材料,采用超高效液相色谱串联质谱(UPLC-MS/MS)技术测定茎秆中花青素苷成分和含量,用紫外可见分光光度计和ELISA试剂盒进行酶活性测定,并分析各花青素苷与酶活性之间的相关性。结果表明,紫花苜蓿红色茎秆中矢车菊素、飞燕草素、天竺葵素、芍药花素及总花青素苷含量显著高于绿色茎秆(P<0.05)。同时,红色茎秆中苯丙氨酸解氨酶(PAL)、肉桂酸4-羟基化酶(C4H)、查尔酮合成酶(CHS)、类黄酮-3-羟化酶(F3H)、谷胱甘肽S-转移酶(GST)活性显著高于绿色茎秆(P<0.05),且矢车菊素、飞燕草素、天竺葵素、芍药花素和总花青素苷含量与PAL、C4H、CHS、F3H和GST活性呈显著或极显著正相关(P<0.05或P<0.01)。综上,花青素苷的种类和含量可能是紫花苜蓿茎秆呈红色的主要原因,PAL、C4H、CHS、F3H和GST活性的提高促进了紫花苜蓿茎秆由绿色变为红色。

关键词：紫花苜蓿空间诱变花青素苷关键酶活性

浙江地区杨梅园土壤重金属含量变异特征与污染风险评价

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果树学报 2017年第4期34卷 473-481页

作者：吕豪豪梁森苗刘玉学江波吕爱华钟哲科杨生茂浙江省农业科学院环境资源与土壤肥料研究所杭州310021 浙江省生物炭工程技术研究中心杭州310021 浙江省农业科学院园艺研究所杭州310021 浙江省林产品质量检测站杭州310023 国家林业局竹子研究开发中心杭州310012

【目的】对浙江省杨梅果园土壤6种重金属含量及动态变化特征进行研究,评价浙江省杨梅园土壤重金属的污染风险。【方法】对2007—2013年连续采集的241个土壤样品中铜、锌、铅、镉、铬、砷和汞等重金属含量进行了分析测定,并通过相关性分... 详细信息

【目的】对浙江省杨梅果园土壤6种重金属含量及动态变化特征进行研究,评价浙江省杨梅园土壤重金属的污染风险。【方法】对2007—2013年连续采集的241个土壤样品中铜、锌、铅、镉、铬、砷和汞等重金属含量进行了分析测定,并通过相关性分析、污染指数等方法分析杨梅园重金属之间的联系及其污染风险。【结果】各个年度杨梅园土壤重金属含量均值较高的为铬和锌,其余依次为铅、铜、砷、汞和镉;除少部分地块一些重金属(汞、砷、铅和锌)指标存在超限值之外,绝大部分地块各指标均低于食用林产品产地土壤环境质量标准。采用单项污染指数和综合污染指数法,对照食用林产品产地环境通用要求中食用林产品产地土壤环境质量标准,对土壤重金属进行了环境质量评价,结果表明,单因子污染指数2008年汞最高,为0.90,锌大部分年度(除2009年和2012年)单项污染指数均超过0.70,处于警戒安全限值。【结论】浙江省杨梅园土壤各项重金属元素的单因子污染指数均小于1,且重金属综合污染指数也均小于1,表明土壤清洁,但锌元素污染指数处于警戒水平,值得持续关注。

关键词：杨梅果园土壤重金属风险评价

苜蓿素对LPS诱导HUVECs炎症反应的消除作用

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甘肃农业大学学报 2024年第3期59卷 1-9页

作者：郭海海范玉雪郭炎峰陈喜宏蒋林峰玉永雄西南大学动物科学技术学院重庆404100 重庆市畜牧推广总站重庆404100

【目的】研究苜蓿素对脂多糖(LPS)诱导的人脐静脉内皮细胞(HUVECs)炎症损伤的保护作用。【方法】以HUVECs为研究对象,用MTT法确定苜蓿素的处理浓度,用酶联免疫分析法确定LPS建模浓度以及检测HUVECs炎症相关因子(TNF-α、iNOS、COX-2、I... 详细信息

【目的】研究苜蓿素对脂多糖(LPS)诱导的人脐静脉内皮细胞(HUVECs)炎症损伤的保护作用。【方法】以HUVECs为研究对象,用MTT法确定苜蓿素的处理浓度,用酶联免疫分析法确定LPS建模浓度以及检测HUVECs炎症相关因子(TNF-α、iNOS、COX-2、IL-1、IL-6)表达,用Western-blot法测定NF-κB和MAPK信号通路蛋白(β-Actin、P65、IKB、P-P65、P-IKB、P38、JNK、ERK1/2、P-P38、P-JNK、P-ERK1/2)表达。【结果】不同浓度的LPS均能促进TNF-α的表达(P<0.01),其中LPS浓度在2.5~7.5μg/mL时TNF-α的表达量最高,选用2.5μg/mL作为建模浓度,在该浓度下LPS促进炎症相关因子和炎症相关通路蛋白表达(P<0.01),而作为阳性对照辛伐他汀能抑制LPS诱导的炎症相关因子和炎症相关通路蛋白表达(P<0.01);苜蓿素浓度在3μg/mL之内对HUVECs活性无显著差异,因而选用3、2、1μg/mL作为苜蓿素高、中、低剂量组;苜蓿素高、中、低剂量组均能下调LPS诱导的炎症相关因子和炎症相关通路蛋白表达(P<0.01),而且苜蓿素对炎症相关因子的抑制效果弱于辛伐他汀,而对炎症相关通路蛋白的抑制效果强于辛伐他汀,各炎症相关因子表达随苜蓿素浓度升高而降低(P<0.01),炎症相关通路蛋白表达情况也有这个下降趋势。【结论】苜蓿素能够通过抑制NF-κB和MAPK信号通路消除LPS对HUVECs诱导的炎症损伤。

关键词：苜蓿素人脐静脉内皮细胞(HUVECs) 炎症脂多糖(LPS)

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E3 ligase Herc4 regulates Hedgehog signalling through promoting Smoothened degradation

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Journal of Molecular Cell Biology 2019年第9期11卷 791-803页

作者： Weirong jiang Xia Yao Zhaoliang Shan Wenting Li yuxue Gao Qing Zhang State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animals for Disease Study Model Animal Research Center of Nanjing UniversityNanjing 210061China

Hedgehog(Hh)signalling plays conserved roles in controlling embryonic development;its dysregulation causes many diseases including *** G protein-coupled receptor Smoothened(Smo)is the key signal transducer of the Hh pathway,whose posttranslational regulation has been shown to be critical for its accumulation and *** has been reported an essential posttranslational regulation of ***,we identify a novel E3 ligase of Smo,Herc4,which binds to Smo,and regulates Hh signalling by controlling Smo ubiquitination and ***,our data suggest that Herc4-mediated Smo degradation is regulated by Hh in PKA-primed phosphorylation-dependent and independent manners.

关键词： Hedgehog Smoothened Herc4 ubiquitination