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Single-cell transcriptomic profiling of non-hematopoietic circulating cells in mid-gestational mouse embryos

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Journal of Genetics and Genomics 2021年第6期48卷 508-511页

作者： Yanyan Li Yanjuan Chen Xiaona zheng Yun Gao yuxuan zheng Zongcheng Li Han He Fuchou Tang Bing Liu Yu Lan Key Laboratory for Regenerative Medicine of Ministry of Education Institute of HematologySchool of MedicineJinan UniversityGuangzhouGuangdong 510632China Integrated Chinese and Western Medicine Postdoctoral Research Station Jinan UniversityGuangzhouGuangdong 510632China Biomedical Institute for Pioneering Investigation via Convergence College of Life SciencesPeking UniversityBeijing 100871China State Key Laboratory of Experimental Hematology Institute of HematologyFifth Medical Center of Chinese PLA General HospitalBeijing 100071China

The circulatory system is indispensable for an animal’s normal *** addition to well-characterized hematopoietic cells,several types of circulating non-hematopoietic cells have been identified in adult mice(Zhang and ... 详细信息

关键词： hematopoietic gestational circulating

Integrative analysis of transcriptome,DNA methylome,and chromatin accessibility reveals candidate therapeutic targets in hypertrophic cardiomyopathy

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Protein & Cell 2024年第11期15卷 796-817页

作者： Junpeng Gao Mengya Liu Minjie Lu yuxuan zheng Yan Wang Jingwei Yang Xiaohui Xue Yun Liu Fuchou Tang Shuiyun Wang Lei Song Lu Wen Jizheng Wang Biomedical Pioneering Innovation Center School of Life SciencesPeking UniversityBeijing 100871China Emergency Center Zhongnan Hospital of Wuhan UniversityWuhan 430071China Beijing Advanced Innovation Center for Genomics(ICG) Ministry of Education Key Laboratory of Cell Proliferation and DifferentiationBeijing 100871China State Key Laboratory of Cardiovascular Disease Fuwai HospitalNational Center for Cardiovascular DiseaseChinese Academy of Medical Science and Peking Union Medical CollegeBeijing 100037China Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesPeking UniversityBeijing 100871China Department of Cardiovascular Surgery Fuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100037China Cardiomyopathy Ward Fuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100037China National Clinical Research Center for Cardiovascular Diseases Fuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100037China

Hypertrophic cardiomyopathy(HCM)is the most common inherited heart disease and is characterized by primary left ventricular hypertrophy usually caused by mutations in sarcomere *** mechanism underlying cardiac remodeling in HCM remains incompletely *** investigation of HCM through integrative analysis at multi-omics levels will be helpful for treating *** methylation and chromatin accessibility,as well as gene expression,were assessed by nucleosome occupancy and methylome sequencing(NOMe-seq)and RNA-seq,respectively,using the cardiac tissues of HCM *** with those of the controls,the transcriptome,DNA methylome,and chromatin accessibility of the HCM myocardium showed multifaceted *** the transcriptome level,HCM hearts returned to the fetal gene program through decreased sarcomeric and metabolic gene expression and increased extracellular matrix gene *** the DNA methylome,hypermethylated and hypomethylated differentially methylated regions were identified in *** the chromatin accessibility level,HCM hearts showed changes in different genome *** transcription factors,including SP1 and EGR1,exhibited a fetal-like pattern of binding motifs in nucleosome-depleted regions in *** particular,the inhibition of SP1 or EGR1 in an HCM mouse model harboring sarcomere mutations markedly alleviated the HCM phenotype of the mutant mice and reversed fetal gene ***,this study not only provides a high-precision multi-omics map of HCM heart tissue but also sheds light on the therapeutic strategy by intervening in the fetal gene reprogramming in HCM.

关键词： hypertrophic cardiomyopathy multi-omics chromatin accessibility DNA methylation fetal gene reprogramming therapy

Goat milk-based infant formula regulates intestinal barrier function and promotes the production of short-chain fatty acids

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Food Science and Human Wellness 2024年第6期13卷 3150-3158页

作者： Qingxue Chen Ting Cao Hongwei Tang Linyi Zhou yuxuan zheng Jinju Cheng Bailiang Li Song Wang College of Food Science Northeast Agricultural UniversityHarbin 150030China College of Food and Health Beijing Technology and Business UniversityBeijing 100048China Key Laboratory for Deep Processing of Major Grain and Oil Ministry of EducationWuhan 430000China Hubei Key Laboratory for Processing and Transformation of Agricultural Products Wuhan 430000China

Infant formula(IF)based on cow milk and goat milk is a substitute food for infants who are underfed with human *** our previous study,we reported the composition and physicochemical stability of IF based on milk from cows and goats and a combination of both ***,we investigated the effects of these 3 IFs on intestinal immunity and short-chain fatty acid production(SCFAs)using human microbiota-associated(HMA)mice and selected human milk as a positive *** results showed that goat milk-based IF is associated with a functional immune advantage,due to the rise in the levels of immune-related cytokines interleukin(IL)-2 and IL-10,decreased levels of intestinal permeability markers D-lactic acid and endotoxin,and increased mRNA levels of intestinal tight junction proteins occludin and *** addition,the intestine of mice fed with goat milk-based IF contained 12.06μmol/g acetate,2.42μmol/g propionate,and 1.72μmol/g butyrate,which reached 69%,79%,and 60%of the levels in human milk,*** results indicate that goat milk-based IF improves intestinal immune function and promotes the production of intestinal SCFAs.

关键词： Goat milk-based infant formula Immune function Short-chain fatty acid Human microbiota associated mice

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慢性萎缩性胃炎脾胃虚弱证中医研究进展

亚太传统医药 2025年第1期21卷 218-223页

作者：郑雨煊王文荣福建中医药大学福建福州350003 福建中医药大学附属第二人民医院福建福州350003

慢性萎缩性胃炎(CAG)是一种发病率较高的胃癌前状态,备受临床关注。中医将CAG分为不同证型,既往多项研究认为,脾胃虚弱证为CAG的发生、传变之源,早期治疗有助于延缓疾病的下一步传变,甚至起到逆转作用。中医治疗随证加减用药,对症状及... 详细信息

慢性萎缩性胃炎(CAG)是一种发病率较高的胃癌前状态,备受临床关注。中医将CAG分为不同证型,既往多项研究认为,脾胃虚弱证为CAG的发生、传变之源,早期治疗有助于延缓疾病的下一步传变,甚至起到逆转作用。中医治疗随证加减用药,对症状及病理改善均起到明显疗效,具有其特色优势,故将中医辨证与现代医学的微生态认识、血清学检查、胃镜检查等相结合,分析各指标及镜下表现等与中医辨证的关联性,并从中医病因病机、用药、选方、外治法等综述CAG脾胃虚弱证的研究进展,以期为后续CAG相关研究提供参考。

关键词：慢性萎缩性胃炎脾胃虚弱证 CAG 中医研究进展

A single-cell transcriptomic atlas of primate pancreatic islet aging

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National Science Review 2021年第2期8卷 17-31页

作者： Jingyi Li yuxuan zheng Pengze Yan Moshi Song Si Wang Liang Sun Zunpeng Liu Shuai Ma Juan Carlos Izpisua Belmonte Piu Chan Qi Zhou Weiqi Zhang Guang-Hui Liu Fuchou Tang Jing Qu State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of Sciences National Laboratory of Biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of Sciences Beijing Institute for Brain Disorders Advanced Innovation Center for Human Brain ProtectionNational Clinical Research Center for Geriatric DisordersXuanwu Hospital Capital Medical University University of Chinese Academy of Sciences Beijing Advanced Innovation Center for Genomics Biomedical Pioneering Innovation CenterCollege of Life SciencesPeking University Ministry of Education Key Laboratory of Cell Proliferation and Differentiation Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesPeking University Institute for Stem Cell and Regeneration Chinese Academy of Sciences The MOH Key Laboratory of Geriatrics Beijing HospitalNational Center of Gerontology State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of Sciences Gene Expression Laboratory Salk Institute for Biological Studies CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences China National Center for Bioinformation

Aging-related degeneration of pancreatic islet cells contributes to impaired glucose tolerance and *** cells age heterogeneously, complicating the efforts to unravel the molecular drivers underlying endocrine aging. To overcome these obstacles, we undertook single-cell RNA sequencing of pancreatic islet cells obtained from young and aged non-diabetic cynomolgus monkeys. Despite sex differences and increased transcriptional variations, aged β-cells showed increased unfolded protein response(UPR) along with the accumulation of protein aggregates. We observed transcriptomic dysregulation of UPR components linked to canonical ATF6 and IRE1 signaling pathways, comprising adaptive UPR during pancreatic aging. Notably, we found aging-related β-cell-specific upregulation of HSP90 B1, an endoplasmic reticulum-located chaperone, impeded high glucose-induced insulin secretion. Our work decodes aging-associated transcriptomic changes that underlie pancreatic islet functional decay at single-cell resolution and indicates that targeting UPR components may prevent loss of proteostasis,suggesting an avenue to delaying β-cell aging and preventing aging-related diabetes.

关键词： islet β-cell aging single-cell RNA sequencing primate

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动态碎裂过程中的最快速卸载现象

中国科学：技术科学 2016年第4期46卷 332-338页

作者：郑宇轩周风华余同希宁波大学冲击与安全工程教育部重点实验室宁波315211 香港科技大学机械与航空航天工程系

固体在高应变率加载作用下常常断裂成多个碎片.Grady针对韧性碎裂和脆性碎裂提出了一个同样形式的碎片尺度预测公式,对韧性碎裂结果预测较好,而对脆性碎裂结果的预测相对较差.是否存在一种物理机制可以同时阐述韧性碎裂和脆性碎裂过程呢... 详细信息

固体在高应变率加载作用下常常断裂成多个碎片.Grady针对韧性碎裂和脆性碎裂提出了一个同样形式的碎片尺度预测公式,对韧性碎裂结果预测较好,而对脆性碎裂结果的预测相对较差.是否存在一种物理机制可以同时阐述韧性碎裂和脆性碎裂过程呢?本文借鉴Grady解决绝热剪切问题时提出的最小集中化时间的思想,认为固体的动态碎裂过程中存在着最快速卸载现象.对韧性碎裂过程和脆性碎裂过程从理论和数值计算上进行分析,发现最快速卸载理论得到的平均碎片尺度和目前文献报道结果均吻合较好.

关键词：碎裂裂纹阵列应力波作用最快速卸载平均碎片尺寸

韧性材料的高应变率拉伸碎裂过程及材料参数影响

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固体力学学报 2012年第4期33卷 358-369页

作者：郑宇轩胡时胜周风华中国科学技术大学中国科学院材料力学行为和设计重点实验室合肥230026 宁波大学教育部冲击与安全工程重点实验室宁波315211

Grady-Kipp将一个与断裂能量相关的内聚断裂模型引入Mott[1]的刚性卸载波分析,导出一个预测韧性材料在高速拉伸碎裂过程中产生碎片的平均尺度的计算公式[2].为定量评估Grady-Kipp公式的适用程度,采用数值方法模拟了具有不同材料参数的... 详细信息

Grady-Kipp将一个与断裂能量相关的内聚断裂模型引入Mott[1]的刚性卸载波分析,导出一个预测韧性材料在高速拉伸碎裂过程中产生碎片的平均尺度的计算公式[2].为定量评估Grady-Kipp公式的适用程度,采用数值方法模拟了具有不同材料参数的一维弹塑性杆在高应变率拉伸过程中的碎裂现象.通过改变材料的断裂能Gc、密度ρ、屈服应力A和应变率敏感系数C,模拟了杆在不同应变率ε下的碎裂过程,研究了材料参数对碎裂时的碎片尺度、表观断裂应变和断口温升等的影响.通过对应变率和碎片尺寸进行无量纲化,证实Grady-Kipp公式在广泛的材料参数范围内能较好地预测碎裂过程中发生的碎片平均尺寸的下限.

关键词：韧性材料碎裂碎片尺度 Grady-Kipp公式 Mott卸载波

The transcription factor AtGLK1 acts upstream of MYBL2 genetically to regulate sucrose-induced anthocyanin biosynthesis in Arabidopsis

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The transcription factor AtGLK1 acts upstream of MYBL2 genet...

江西省作物学会2020年学术年会

作者： Dongming Zhao yuxuan zheng Ziyu Yao Yongjun Zeng Dong Liu Key Laboratory of Crop Physiology Ecology and Genetic Breeding Ministry of Education Jiangxi Agricultural University

The regulation of anthocyanin biosynthesis by various factors including sugars, light and abiotic stresses is mediated by numerous regulatory factors acting at the transcriptional level. Here we provide experimental evidence to show that AtGLK1, the nuclear GARP transcription factor, plays an important role in regulating anthocyanin biosynthesis in Arabidopsis. Real-time quantitative PCR and GUS staining assays demonstrated that AtGLK1 was mainly expressed in the green tissues of Arabidopsis seedlings and could be induced by sucrose. The loss-of-function glk1 glk2 double mutant have lower anthocyanin levels than glk2 single mutant, although loss of AtGLK1 alone does not affect anthocyanin accumulation. Overexpression of AtGLK1 enhances the accumulation of anthocyanin in transgenic Arabidopsis seedlings accompanied by increased expression of anthocyanin biosynthetic and regulatory genes. Moreover, we found that AtGLK1 participates in the plastid-signalling-mediated anthocyanin accumulation. Genetic, physiological and molecular biological approaches demonstrated that AtGLK1 acts upstream of MYBL2, a key negative regulator of anthocyanin biosynthesis, genetically to regulate sucrose-induced anthocyanin biosynthesis. Taken together, these results indicate that AtGLK1 positively regulates sucrose-induced anthocyanin biosynthesis in Arabidopsis via repression of MYBL2.

关键词： Arabidopsis thaliana Anthocyanin AtGLK1 MYBL2

来源： cnki会议评论

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固体的冲击拉伸碎裂

力学进展 2016年第1期46卷 506-540页

作者：郑宇轩周风华胡时胜余同希宁波大学教育部冲击与安全工程重点实验室宁波315211 中国科学技术大学中科院材料力学行为和设计重点实验室合肥230027 香港科技大学机械与航空航天工程系香港999077

固体材料在冲击拉伸载荷作用下常常会断裂成多个碎片(碎片化),固体材料碎片化的物理机制是多点损伤同时在固体中成核和发展,导致固体多处破坏.自Mott对固体的动态碎裂问题进行了开创性研究后,几十年来,对固体动态碎裂机制的研究一直是... 详细信息

固体材料在冲击拉伸载荷作用下常常会断裂成多个碎片(碎片化),固体材料碎片化的物理机制是多点损伤同时在固体中成核和发展,导致固体多处破坏.自Mott对固体的动态碎裂问题进行了开创性研究后,几十年来,对固体动态碎裂机制的研究一直是应用物理学、力学、航天和兵器工程等领域共同关心的重要课题.本文介绍了在冲击拉伸载荷作用下固体的动态碎裂研究的发展历史,给出相关的理论分析、实验研究和数值模拟的研究进展,特别针对现有的各种关于碎片尺度、碎片分布、以及碎片化物理机制的理论模型进行了较详尽的阐述和讨论,最后指出现有实验和理论研究中仍然存在的关键科学问题及进一步的研究展望.

关键词：冲击拉伸碎裂脆性和韧性断裂碎片尺度碎片分布