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Neural Regeneration Research 2014年第22期9卷 1961-1967页

作者： Li Zhao Wei Qu yuxuan wu Hao Ma Huajun Jiang Department of Anesthesiology Heji Hospital Affiliated to Changzhi Medical College Department of Orthopedics First Affiliated Hospital of Dalian Medical University

Schwann cells, nerve regeneration promoters in peripheral nerve tissue engineering, can be used to repair both the peripheral and central nervous systems. However, isolation and puriifcation of Schwann cells are complicated by contamination with ifbroblasts. Current reported measures are mainly limited by either high cost or complicated procedures with low cell yields or purity. In this study, we collected dorsal root ganglia from neonatal rats from which we obtained highly puriifed Schwann cells using serum-free melanocyte culture medium. The purity of Schwann cells （〉95%） using our method was higher than that using standard medium containing fetal bovine serum. The obtained Schwann cells were implanted into poly（lactic-co-glycolic acid）/chi-tosan conduits to repair 10-mm sciatic nerve defects in rats. Results showed that axonal diameter and area were signiifcantly increased and motor functions were obviously improved in the rat sciatic nerve tissue. Experimental ifndings suggest that serum-free melanocyte culture medium is conducive to purify Schwann cells and poly（lactic-co-glycolic acid）/chitosan nerve conduits combined with Schwann cells contribute to restore sciatic nerve defects.

关键词： nerve regeneration Schwann cells dorsal root ganglia melanocyte medium fibroblasts poly(lactic-co-glycolic acid) chitosan sciatic nerve defect NSFC grants neural regeneration

Combined blasting for protection of gob-side roadway with thick and hard roof

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第8期16卷 3165-3180页

作者： Qiang Fu Jun Yang Yubing Gao Changjiang Li Hongxu Song yuxuan Liu Xing wu State Key Laboratory for Tunnel Engineering China University of Mining and Technology(Beijing)Beijing100083China School of Mechanics and Civil Engineering China University of Mining and Technology(Beijing)Beijing100083China

The deformation control of surrounding rock in gobside roadway with thick and hard roof poses a significant challenge to the safety and efficiency of coal *** address this issue,a novel approach combining directional and non-directional blasting techniques,known as combined blasting,was *** study focuses on the experimental investigation of the proposed method in the 122108 working face in Caojiatan Coal Mine as the engineering *** initial phase of the study involves physical model experiments to reveal the underlying mechanisms of combined blasting for protecting gob-side roadway with thick and hard *** results demonstrate that this approach effectively accelerates the collapse of thick and hard roofs,enhances the fragmentation and expansion coefficient of gangue,facilitates the filling of the goaf with gangue,and provides support to the overlying strata,thus reducing the subsidence of the overlying strata above the ***,the method involves cutting the main roof into shorter beams to decrease the stress and disrupt stress transmission *** numerical simulations were conducted to corroborate the findings of the physical model experiments,thus validating the accuracy of the experimental ***,field engineering experiments were performed,affirming the efficacy of the combined blasting method in mitigating the deformation of surrounding rock and achieving the desired protection of the gob-side roadway.

关键词： Thick and hard roof Surrounding rock control Combined blasting Fragmentation and expansion support stress relief

Targeting lipid metabolism overcomes BTK inhibitor resistance in diffuse large B-cell lymphoma

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Medicine in Novel Technology and Devices 2024年第2期22卷 9-14页

作者： Zhuojun Liu Chenyue wu Zhaohua Yao yuxuan Wang Zhe Yu Jian Yu School of Biological Science and Medical Engineering Beihang UniversityBeijing100191China Key Laboratory of Biomechanics and Mechanobiology Beijing Advanced Innovation Center for Biomedical EngineeringSchool of Biological Science and Medical EngineeringBeihang UniversityBeijing100191China Shandong Provincial Third Hospital Shandong250031China

Exploring novel therapeutic targets of Diffuse large B cell lymphoma(DLBCL)to overcome its resistance to Bruton's Tyrosine Kinase(BTK)inhibitors is a key *** metabolism,crucial in the resistance landscape of various cancers,may unlock new aspects of BTK inhibitor resistance in *** the known importance of lipid metabolism in oncological resistance patterns,its intricate role in DLBCL and its resistance to BTK inhibitor therapies remains largely *** review will explore the complex relationship between lipid metabolism and BTK inhibitor resistance,giving new insights for improving the effectiveness of BTK inhibitors and developing innovative combination treatment methods for *** review holds the potential for guiding future research directions,optimizing existing therapeutic processes,and inspiring the development of innovative combinatorial therapeutic strategies that improve outcomes in patients with DLBCL.

关键词： Diffuse large B-Cell lymphoma BTK inhibitor resistance Lipid metabolism Fatty acid Cholesterol

Overview of recognition methods for SSVEP-based BCIs in World Robot Contest 2022: MATLAB undergraduate group

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Brain Science Advances 2023年第3期9卷 224-236页

作者： Chengzhi Yi yuxuan wu Fan Ye Xinchen Zhang Jingjing Chen Department of Biomedical Engineering School of MedicineTsinghua UniversityBeijing 100084China College of Science Beijing Forestry UniversityBeijing 100083China Department of Psychology School of Social SciencesTsinghua UniversityBeijing 100084China Tsinghua Laboratory of Brain and Intelligence Tsinghua UniversityBeijing 100084China

The steady-state visual evoked potential(SSVEP)-based speller has emerged as a widely adopted paradigm in current brain–computer interface(BCI) systems due to its rapid processing and consistent performance across different individuals. Calibration-free SSVEP algorithms, as opposed to their calibration-based counterparts, offer clear and intuitive mathematical principles, making them accessible to novice developers. During the World Robot Contest(WRC)2022, participants in the undergraduate category utilized various approaches to accomplish target detection in the calibration-free setting, successfully implementing the algorithms using *** winning approach achieved an average information transfer rate of 198.94 bits/min in the final test, which is notably high given the calibration-free scenario. This paper presents an introduction to the underlying principles of the selected methods, accompanied by a comparison of their effectiveness through analysis of results from both the final test and offline experiments. Additionally, we propose that the youth competition of WRC could serve as an ideal starting point for beginners interested in studying and developing their own BCI systems.

关键词： brain-computer interfaces electroencephalogram steady-state visual evoked potential BCI spellers calibration-free MATLAB

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空气炸鲨鱼皮工艺条件的优化

渔业研究 2024年第3期46卷 260-270页

作者：陈晓婷吴雨璇刘淑集潘南许旻苏永昌张玉苍刘智禹集美大学海洋食品与生物工程学院福建厦门361021 福建水产研究所国家海水鱼类加工技术研发中心福建省海洋生物增养殖与高值化利用重点实验室福建厦门361013

为开发鲨鱼皮即食休闲食品,本文对鲨鱼皮进行空气炸工艺条件的优化。采用鲨鱼皮为原料,以质构特性和色泽变化为指标,通过预干燥控制鲨鱼皮水分含量,确定最佳预干燥条件;并在单因素试验的基础上进行正交实验优化,研究碱液浓度、泡碱时间... 详细信息

为开发鲨鱼皮即食休闲食品,本文对鲨鱼皮进行空气炸工艺条件的优化。采用鲨鱼皮为原料,以质构特性和色泽变化为指标,通过预干燥控制鲨鱼皮水分含量,确定最佳预干燥条件;并在单因素试验的基础上进行正交实验优化,研究碱液浓度、泡碱时间、空气炸温度和空气炸时间对鲨鱼皮品质的影响。结果表明,当预干燥控制水分含量在30%~60%时,鱼皮具有膨化作用,确定空气炸鲨鱼皮最佳工艺条件为预干燥温度80℃、干燥时间90 min、碱液浓度15 g·L^(-1)、泡碱时间10 min、空气炸温度150℃、空气炸时间14 min。该条件下制备的鲨鱼皮膨化效果最佳、色泽均匀、脆度和酥度较好。本研究旨在为空气炸鲨鱼皮即食产品工艺提供一定的理论基础。

关键词：鲨鱼皮预干燥空气炸品质工艺优化

Spermatogenic Cell-Specific Gene Mutation in Mice via CRISPR-Cas9

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Journal of Genetics and Genomics 2016年第5期43卷 289-296页

作者： Meizhu Bai Dan Liang Yinghua Wang Qing Li yuxuan wu Jinsong Li School of Life Science and Technology Shanghai Tech University Group of Epigenetic Reprogramming State Key Laboratory of Cell BiologyCAS Center for Excellence in Molecular Cell ScienceInstitute of Biochemistry and Cell BiologyShanghai Institutes for Biological SciencesChinese Academy of Sciences Shanghai Key Laboratory of Molecular Andrology Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological SciencesChinese Academy of Sciences

Tissue-specific knockout technology enables the analysis of the gene function in specific tissues in adult ***,conventional strategy for producing tissue-specific knockout mice is a time- and labor-consuming process,restricting rapid study of the gene function in ***-Cas9 system from bacteria is a simple and efficient gene-editing technique,which has enabled rapid generation of gene knockout lines in mouse by direct injection of CRISPR-Cas9 into ***,we demonstrate CRISPR-Cas9-mediated spermatogenic cell-specific disruption of Scp3 gene in testes in one *** first generated transgenic mice by pronuclear injection of a plasmid containing Hspa2 promoter driving Cas9 expression and showed Cas9 specific expression in spermatogenic *** then produced transgenic mice carrying Hspa2 promoter driven Cas9 and constitutive expressed sgRNA targeting Scp3 *** founders were infertile due to developmental arrest of spermatogenic cells while female founders could produce progeny ***,male progeny from female founders were infertile and females could transmit the transgenes to the next *** study establishes a CRISPR-Cas9-based one-step strategy to analyze the gene function in adult tissues by a temporal-spatial pattern.

关键词： CRISPR-Cas9 Tissue-specific knockout Spermatogenesis Mouse

Anodic Electrosynthesis of Amide from Alcohol and Ammonia

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CCS Chemistry 2024年第1期6卷 125-136页

作者： yuxuan Lu Yingying Li Bo Zhou Jingcheng wu Ling Zhou Yuping Pan Zhongcheng Xia Ming Yang Yandong wu Zhenran Yuan Rixin Peng Zhijie Kong Shuangyin Wang Yuqin Zou State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringAdvanced Catalytic Engineering Research Center of the Ministry of EducationHunan UniversityChangsha 410082 Shenzhen Institute of Hunan University Shenzhen 518057 School of Chemistry and Chemical Engineering Jishou UniversityJishouHunan 416000

Amide is essential in biologically active compounds,synthetic materials,and building ***,conventional amide production relies on energyintensive consumption and activating agents that modulate processes to construct the C–N ***,for the first time,we have successfully realized the formation of amides at industrial current density via the anodic coelectrolysis of alcohol and ammonia under ambient *** have proved thatmodulation of the interface microenvironment concentration of nucleophile by electrolyte engineering can regulate the reaction pathways of amides rather than acetic *** C-N coupling strategy can be further extended to the electrosynthesis of the long-chain and aryl-ring amide with high selectivity by replacing ammonia with *** work opens up a vast store of information on the utilization of biomass alcohol for high-value N-containing chemicals via an electrocatalytic C-N coupling reaction.

关键词： electrocatalysis electrocatalysts biomass upgrading elctrochemical oxidation coupling amide production

Luminescent metal-halide perovskites:fundamentals,synthesis,and light-emitting devices

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Science China Chemistry 2024年第6期67卷 1776-1838页

作者： Kang Wang Jeong Hui Kim Jie Yang Xiaoke Liu Yixuan Dou yuxuan Li Weijian Tao Haiyun Dong Haiming Zhu Kaifeng wu Li Na Quan Feng Gao Jianpu Wang Letian Dou Yong Sheng Zhao Beijing National Laboratory for Molecular Sciences(BNLMS) Key Laboratory of PhotochemistryInstitute of ChemistryChinese Academy of SciencesBeijing100190China Davidson School of Chemical Engineering Purdue UniversityWest LafayetteIN47907USA School of Chemical Sciences University of Chinese Academy of SciencesBeijing100049China Key Laboratory of Flexible Electronics(KLOFE) Institute of Advanced Materials(IAM)&School of Flexible Electronics(Future Technologies)Nanjing Tech University(NanjingTech)Nanjing211816China Department of Physics Linköping UniversityLinköping58183Sweden Department of Chemistry Virginia TechBlacksburgVA24060USA State Key Laboratory of Molecular Reaction Dynamics Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian116023China Department of Chemistry Zhejiang UniversityHangzhou310058China Changzhou University Changzhou213164China Department of Chemistry Purdue UniversityWest LafayetteIN47907USA

Metal-halide perovskites have garnered considerable research attention as highly efficient light emitters in recent years due to their outstanding optoelectronic properties with remarkable tunability and excellent solution *** advancements have been achieved in the development of novel halide perovskites,and the exploitations of these materials in lightemitting *** review comprehensively outlines recent breakthroughs in metal-halide perovskites,encompassing the rational design of perovskite materials with tunable light emission properties,the controllable growth of single crystal for a deeper understanding of their structure-property relationships,as well as the fundamental insights into the photophysics and carrier dynamics in perovskite ***,it provides an overview of recent applications of perovskite materials in high-performance light-emitting diodes(LEDs)and lasers.

关键词： metal-halide perovskites perovskite luminescent materials perovskite synthesis excited-state processes perovskite light-emitting diodes perovskite lasers

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边缘、对抗与渴望:马来西亚华裔英语作家文化身份构建研究

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当代外国文学 2023年第1期44卷 87-94页

作者：乔幪吴雨轩宁夏大学外国语学院华北理工大学国际教育中心北京语言大学外国语学部

马来西亚华裔英语作家流散时间久远、创作身份独特,但学界尚未对其展开整体研究。选取林玉玲等六位作家的作品,考察作家与侨居国、西方及祖籍国的关系对其文化身份构建产生的影响。研究发现,马来西亚华裔英语作家对侨居国有很深的感情牵... 详细信息

马来西亚华裔英语作家流散时间久远、创作身份独特,但学界尚未对其展开整体研究。选取林玉玲等六位作家的作品,考察作家与侨居国、西方及祖籍国的关系对其文化身份构建产生的影响。研究发现,马来西亚华裔英语作家对侨居国有很深的感情牵绊,也因此激烈地批判侨居国对本族裔的边缘化;他们接受英语教育、用英语创作,但马来西亚的殖民经历又使其有意识地对抗西方文化的优越性,体现出作家的主体性;他们因缺失而产生对中华文化的渴望,而且中华民族文化资本依然可以为其在困境中提供精神支持。穿行于三种文化间使其身份体现出的高度杂糅性,因此,探索流散族裔文化身份时,研究者要看到流散经历带来的变化,也要重视根文化的滋养作用。

关键词：马来西亚华裔英语作家流散文化身份杂糅性

Utilizing Certificateless Cryptography for IoT Device Identity Authentication Protocols in Web3

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ZTE Communications 2024年第2期22卷 30-38页

作者： wu Zhihui HONG yuxuan ZHOU Enyuan LIU Lei PEI Qingqi Guangzhou Institute of Technology Xidian UniversityGuangzhou 510700China Guangzhou Lianrong Information Technology Co.Ltd. Guangzhou 510700China The Hong Kong Polytechnic University Hong Kong 999077China

Traditional methods of identity authentication often rely on centralized architectures,which poses risks of computational overload and single points of *** propose a protocol that offers a decentralized approach by distributing authentication services to edge authentication gateways and servers,facilitated by blockchain technology,thus aligning with the decentralized ethos of Web3 ***,we enhance device security against physical and cloning attacks by integrating physical unclonable functions with certificateless cryptography,bolstering the integrity of Internet of Thins(IoT)devices within the evolving landscape of the *** achieve dynamic anonymity and ensure privacy within Web3 environments,we employ fuzzy extractor technology,allowing for updates to pseudonymous identity identifiers while maintaining key *** proposed protocol ensures continuous and secure identity authentication for IoT devices in practical applications,effectively addressing the pressing security concerns inherent in IoT network environments and contributing to the development of robust security infrastructure essential for the proliferation of IoT devices across diverse settings.

关键词： blockchain certificateless cryptography identity authentication IoT

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