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CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta Variant

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Nano-Micro Letters 2022年第10期14卷 83-94页

作者： Chenshuo Wu Zhi Chen Chaozhou Li Yabin Hao yuxuan tang yuxuan Yuan Luxiao Chai Taojian Fan Jiangtian Yu Xiaopeng Ma Omar AAl-Hartomy SWageh Abdullah GAl-Sehemi Zhiguang Luo Yaqing He Jingfeng Li Zhongjian Xie Han Zhang International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of EducationInstitute of Microscale OptoelectronicsCollege of Physics and Optoelectronic EngineeringShenzhen UniversityShenzhen 518060People’s Republic of China Shenzhen International Institute for Biomedical Research Shenzhen 518116People’s Republic of China Department of Respiratory Shenzhen Children’s HospitalShenzhen 518038People’s Republic of China Institute of Pediatrics Shenzhen Children’s HospitalShenzhen 518038People’s Republic of China Shenzhen Han’s Tech Limited Company Shenzhen 518000People’s Republic of China Hospital of Guangzhou Medical University Qingyuan city People’s HospitalQingyuan 511518People’s Republic of China Department of Physics Faculty of ScienceKing Abdulaziz UniversityJeddah 21589Saudi Arabia Research Center for Advanced Materials Science(RCAMS) King Khalid UniversityP.O.Box 9004Abha 61413Saudi Arabia Department of Chemistry College of ScienceKing Khalid UniversityP.O.Box 9004Abha 61413Saudi Arabia Zhongmin(Shenzhen)Intelligent Ecology Co. LtdShenzhen 518055People’s Republic of China Shenzhen Center for Disease Control and Prevention Shenzhen 518055People’s Republic of China

Coronavirus disease 2019(COVID-19)is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).The gold standard method for the diagnosis of SARS-CoV-2 depends on quantitative reverse transcription-polymerase chain reaction till now,which is time-consuming and requires expensive instrumentation,and the confirmation of variants relies on further sequencing ***,we first proposed a robust technique-methodology of electrochemical CRISPR sensing with the advantages of rapid,highly sensitivity and specificity for the detection of SARS-CoV-2 *** enhance the sensing capability,gold electrodes are uniformly decorated with electro-deposited gold *** DNA template identical to SARS-CoV-2 Delta spike gene sequence as model,our biosensor exhibits excellent analytical detection limit(50 fM)and high linearity(R2=0.987)over six orders of magnitude dynamic range from 100 fM to 10 nM without any nucleic-acid-amplification *** detection can be completed within 1 h with high stability and specificity which benefits from the CRISPR-Cas ***,based on the wireless micro-electrochemical platform,the proposed biosensor reveals promising application ability in point-of-care testing.

关键词： SARS-COV-2 variant Methodology of electrochemical CRISPR sensing(MOECS) Gold nanoparticles(AuNPs) Point-of-care testing(POCT)

Arbuscular mycorrhizal fungi inoculation and exogenous indole-3-acetic acid application induce antioxidant defense response to alleviate cadmium toxicity in Broussonetia papyrifera

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Pedosphere 2024年第2期34卷 447-459页

作者： Xue LI Jingwei LIANG Hongjian WEI yuxuan KUANG Hui CHEN Ming tang Wentao HU State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources Guangdong Laboratory for Lingnan Modern AgricultureGuangdong Key Laboratory for Innovative Development and Utilization of Forest Plant GermplasmCollege of Forestry and Landscape ArchitectureSouth China Agricultural UniversityGuangzhou 510642China

Cadmium(Cd)contamination in soil poses a huge threat to plants even at low concentrations;Broussonetia papyrifera has great potential in remediation of soil heavy metal ***,whether exogenous indole-3-acetic acid(IAA)application and arbuscular mycorrhizal fungi(AMF)have synergistic effects on Cd tolerance of *** remains *** investigate the effects of AMF inoculation and IAA application on the tolerance of *** to Cd stress,two experiments were conducted:the first to investigate the effect of AMF(Rhizophagus irregularis)inoculation on the tolerance of *** to Cd stress and the second to investigate the combined effects of AMF inoculation and IAA application on the tolerance of *** to Cd *** including endogenous hormone concentration,antioxidant defense response,malondialdehyde(MDA)content,and gene expression related to antioxidant enzyme system and hormone were *** results indicated that AMF alleviated Cd toxicity of *** by reducing MDA content and improving antioxidant enzyme activities and Cd absorption ***,the combination of AMF inoculation and IAA application had a synergetic effect on the tolerance of *** to Cd stress through upregulating BpAUX1 and BpAUX2,which might contribute to root growth and root xylem synthesis,and by upregulating BpSOD2 and BpPOD34 to enhance the antioxidant enzyme *** work provides a new insight into the application of IAA in the remediation of soil Cd pollution by mycorrhizal plants.

关键词： enzyme activity geneexpression heavy metal phytoremediation remediation Rhizophagus irregularis soil cadmium pollution stress tolerance

不同棉花-荷兰豆套种模式下作物生育进程、产量及综合效益的比较分析

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中国棉花 2024年第6期51卷 37-42页

作者：常明娟楚宗艳杜玉倍汤玉煊吴超占亚楠刘素玲开封市农林科学研究院河南开封475004

为提升植棉效益和土地利用率,降低荷兰豆种植成本,以早熟棉花品种中棉所50和荷兰豆品种金船白花双荚818为材料,于2022―2023年在河南省开封市设置了不同的棉花-荷兰豆套作模式,比较分析其对棉花和荷兰豆的生育进程、产量、经济效益等的... 详细信息

为提升植棉效益和土地利用率,降低荷兰豆种植成本,以早熟棉花品种中棉所50和荷兰豆品种金船白花双荚818为材料,于2022―2023年在河南省开封市设置了不同的棉花-荷兰豆套作模式,比较分析其对棉花和荷兰豆的生育进程、产量、经济效益等的影响。结果表明:棉花种植密度(8.34万株·hm^(-2))相同时,80 cm等行距种植(A1)、(120+40) cm宽窄行种植(A2)和(100+60) cm宽窄行种植(A3)的铃重、衣分、籽棉单产和皮棉单产均无显著差异,棉花生育进程有所不同。将2行荷兰豆套种于每2等行棉花中间或2宽行棉花中间,分别距棉花植株15 cm(B1)或20 cm(B2),该模式的荷兰豆种植成本较传统种植模式降低4 400元·hm^(-2)。不同套种模式的荷兰豆累计采摘量均呈“S”曲线,其中A1B1处理为最优的套种模式,荷兰豆单日采摘量最大值为341.84 kg·hm^(-2),荷兰豆累计采摘量为9 093.09 kg·hm^(-2),棉花和荷兰豆的总产值及综合纯收益最高,分别达63 944.68元·hm^(-2)和31 894.66元·hm^(-2)。

关键词：棉花-荷兰豆套作行距配置生育进程群体产量综合效益

Low Resource Chinese Geological Text Named Entity Recognition Based on Prompt Learning

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Journal of Earth Science 2024年第3期35卷 1035-1043页

作者： Hang He Chao Ma Shan Ye Wenqiang tang yuxuan Zhou Zhen Yu Jiaxin Yi Li Hou Mingcai Hou State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Institute of Sedimentary GeologyCollege of Computer Science and Cyber SecurityChengdu University of TechnologyChengdu 610059China Key Laboratory of Deep-Time Geography and Environment Reconstruction and Applications of Ministry of Natural Resources Chengdu University of TechnologyChengdu 610059China School of Information Engineering China University of Geosciences(Beijing)Beijing 100083China

Geological reports are a significant accomplishment for geologists involved in geological investigations and scientific research as they contain rich data and textual *** the rapid development of science and technology,a large number of textual reports have accumulated in the field of ***,many non-hot topics and non-English speaking regions are neglected in mainstream geoscience databases for geological information mining,making it more challenging for some researchers to extract necessary information from these *** Language Processing(NLP)has obvious advantages in processing large amounts of textual *** objective of this paper is to identify geological named entities from Chinese geological texts using NLP *** propose the RoBERTa-Prompt-Tuning-NER method,which leverages the concept of Prompt Learning and requires only a small amount of annotated data to train superior models for recognizing geological named entities in low-resource dataset *** RoBERTa layer captures context-based information and longer-distance dependencies through dynamic word ***,we conducted experiments on the constructed Geological Named Entity Recognition(GNER)*** experimental results show that the proposed model achieves the highest F1 score of 80.64%among the four baseline algorithms,demonstrating the reliability and robustness of using the model for Named Entity Recognition of geological texts.

关键词： Prompt Learning Named Entity Recognition(NER) low resource geological text text information mining big data geology.

Immunotherapy for Small Cell Lung Cancer

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Journal of Biosciences and Medicines 2024年第8期12卷 320-332页

作者： yuxuan Ge Xi tang Department of Thoracic Oncology Jingzhou Hospital Affiliated to Yangtze University Jingzhou China

Small cell lung cancer (SCLC) is a poorly differentiated, highly malignant neuroendocrine tumor characterized by rapid growth, aggressiveness, and easy recurrence. It is usually found in late clinical stage and the opportunity for surgery is lost. Therefore, surgery is often not used in clinical treatment. Although it is sensitive to chemoradiotherapy, it has a high recurrence rate and lacks effective treatment methods at present. Following chemotherapy and radiotherapy, immunotherapy for small cell lung cancer has become the mainstream research direction. Immunotherapy is profoundly changing the approach to cancer treatment due to its tolerable safety profile, sustained treatment response due to the production of immune memory, and effectiveness in a broad patient population. Immunotherapy for small cell lung cancer is one of the effective treatment methods for small cell lung cancer, and relevant studies are not rare, but there are still shortcomings such as intolerance of side effects and inaccurate evaluation of treatment timing. This article reviews the history of immunotherapy, the mechanism of action of immunodrugs, and the current immunodrugs used in the first-line treatment of extensive small cell lung cancer.

关键词： Small Cell Lung Cancer Immunotherapy PD-L1 Inhibitor PD-1 Inhibitor

适宜早熟棉—荷兰豆套作的荷兰豆品种筛选试验

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蔬菜 2024年第1期 64-67页

作者：杜玉倍楚宗艳常明娟吴超占亚楠汤玉煊刘素玲周玉琴司啸宏开封市农林科学研究院河南开封475004

为稳定豫东地区棉花种植面积,提高复种指数,降低种植成本,提高农民经济收益,引进5个荷兰豆品种,以筛选出适宜在豫东地区和早熟棉套作的荷兰豆品种。结果显示,口感脆嫩、产量高的金船白花双荚818和豌豆6号,开花时间比较集中,便于采摘,株... 详细信息

为稳定豫东地区棉花种植面积,提高复种指数,降低种植成本,提高农民经济收益,引进5个荷兰豆品种,以筛选出适宜在豫东地区和早熟棉套作的荷兰豆品种。结果显示,口感脆嫩、产量高的金船白花双荚818和豌豆6号,开花时间比较集中,便于采摘,株高和棉株相近,适宜攀附在早熟棉植株上进行套作;早熟棉套种金船白花双荚818综合经济效益为50223元/hm^(2),套种豌豆6号综合经济效益为49 319元/hm^(2),效益可观,值得在豫东地区大力推广。

关键词：早熟棉荷兰豆品种筛选

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豫东植棉区几种棉花轮作套作模式

中国棉花 2024年第6期51卷 60-62页

作者：杜玉倍楚宗艳汤玉煊常明娟吴超占亚楠刘素玲司啸宏周玉琴开封市农林科学研究院河南开封475004

近年来豫东地区棉花种植面积持续下滑,而开展棉花高效多熟种植,提高复种指数,是稳定植棉面积、提高棉农收益的有效途径之一。介绍了适宜豫东地区种植的棉花轮作套种模式及优点,包括“早熟棉-小麦”轮作模式、“棉花-西瓜”套作模式、“... 详细信息

近年来豫东地区棉花种植面积持续下滑,而开展棉花高效多熟种植,提高复种指数,是稳定植棉面积、提高棉农收益的有效途径之一。介绍了适宜豫东地区种植的棉花轮作套种模式及优点,包括“早熟棉-小麦”轮作模式、“棉花-西瓜”套作模式、“棉花-荷兰豆”套作模式、“早熟棉-花生-大蒜”套作模式。

关键词：棉花套种轮作小麦荷兰豆西瓜

Efficacy and safety of low-dose cyclophosphamide combined with lenvatinib, pembrolizumab and TACE for unresectable hepatocellular carcinoma:A single-center, prospective,single-arm clinical trial

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Chinese Journal of Cancer Research 2024年第2期36卷 114-123页

作者： Yupeng Ren yuxuan Li Mingbo Cao Yongchang tang Feng Yuan Gaoyuan Yang Zhiwei He Zheng Shi Xiaorui Su Zhicheng Yao Meihai Deng Department of Hepatobiliary Surgery the Third Affiliated HospitalSun Yat-sen UniversityGuangzhou 510630China Department of General Surgery Qilu HospitalShandong UniversityJinan 250012China Department of Hepatobiliary Surgery the First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510120China Department of Hepatobiliary and Pancreatic Surgery the Third Affiliated HospitalSun Yat-sen UniversityGuangzhou 510630China

Objective: Unresectable hepatocellular carcinoma(uHCC) continues to pose effective treatment options. The objective of this study was to assess the efficacy and safety of combining low-dose cyclophosphamide with lenvatinib, pembrolizumab and transarterial chemoembolization(TACE) for the treatment of ***: From February 2022 to November 2023, a total of 40 patients diagnosed with uHCC were enrolled in this small-dose, single-center, single-arm, prospective study. They received a combined treatment of low-dose cyclophosphamide with lenvatinib, pembrolizumab, and TACE. Study endpoints included progression-free survival(PFS), objective response rate(ORR), and safety assessment. Tumor response was assessed using the modified Response Evaluation Criteria in Solid Tumors(mRECIST), while survival analysis was conducted through KaplanMeier curve analysis for overall survival(OS) and PFS. Adverse events(AEs) were evaluated according to the National Cancer Institute Common Terminology Criteria for Adverse Events(version 5.0).Results: A total of 34 patients were included in the study. The median follow-up duration was 11.2 [95% confidence interval(95% CI), 5.3-14.6] months, and the median PFS(mPFS) was 15.5(95% CI, 5.4-NA) *** OS(mOS) was not attained during the study period. The ORR was 55.9%, and the disease control rate(DCR) was 70.6%. AEs were reported in 27(79.4%) patients. The most frequently reported AEs(with an incidence rate >10%) included abnormal liver function(52.9%), abdominal pain(44.1%), abdominal distension and constipation(29.4%), hypertension(20.6%), leukopenia(17.6%), constipation(17.6%), ascites(14.7%), and insomnia(14.7%). Abnormal liver function(14.7%) had the most common grade 3 or higher ***: A combination of low-dose cyclophosphamide with lenvatinib, pembrolizumab, and TACE is safe and effective for u HCC, showcasing a promising therapeutic strategy for managing uHCC.

关键词： Hepatocellular carcinoma lenvatinib low-dose cyclophosphamide pembrolizumab transarterial chemoembolization

Wastewater surveillance and an automated robot:effectively tracking SARS-CoV-2 transmission in the post-epidemic era

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National Science Review 2023年第6期10卷 86-95页

作者： Guanyong Ou yuxuan tang Shiyu Niu Liwen Wu Shaxi Li Yang Yang Jun Wang Yun Peng Chuanfu Huang Wei Hu Qinghua Hu Yinghui Li Yang Ping Chao Lin Boping Yu Qi Han Yabin Hao Zhiguang Luo Wende Tian Han Zhang Yingxia Liu National Clinical Research Center for Infectious Disease State Key Discipline of Infectious Disease The Third People's Hospital of Shenzhen Second Hospital Affiliated to Southern University of Science and Technology School of Medicine Southern University of Science and Technology International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education Institute of Microscale Optoelectronics College of Physics and Optoelectronic Engineering Shenzhen University Shenzhen Longhua Drainage Co. Ltd. Shenzhen Center for Disease Control and Prevention Microbiology Laboratory Shenzhen Center for Disease Control and Prevention Power China Eco-Environmental Group Co. Ltd. Shenzhen Water Planning & Design Institute Co. Ltd. Shenzhen Academy of Environmental Sciences School of Civil and Environmental Engineering Harbin Institute of Technology (Shenzhen) 1Institute of Solid Wastes and Physical Environment Research Shenzhen Academy of Environmental Sciences Shenzhen Metasensing Technology Co. Ltd. 13. Zhongmin (Shenzhen) intelligent ecology Co. Ltd. Research Centre for Eco-Environmental Sciences Chinese Academy of Sciences

Wastewater-based epidemiology(WBE) has exhibited great utility in the early and rapid identification of SARS-C ***,the efficacy of wastewater surveillance under China’ s previous strict epidemic prevention policy remains to be *** collected the WBE data of wastewater treatment plants(WWTPs) in the Third People’s Hospital of Shenzhen and several communities to determine the significant effectiveness of routine wastewater surveillance in monitoring the local spread of SARS-C oV-2under tight containment of the *** results of 1 month of continuous wastewater surveillance showed that positive signals for SARS-C oV-2 RNA were detected in the wastewater samples,and a significant positive correlation was observed between the virus concentration and the number of daily *** addition,the community’ s domestic wastewater surveillance results were confirmed even 3 days before,or simultaneously with,the infected patient being confirmed as having the ***,an automated sewage virus detection robot,ShenNong No.l robot,was developed,showing a high degree of agreement with experimental data,offering the possibility of large-scale multi-point ***,our results illustrated the clear indicative role of wastewater surveillance in combating COVID-19 and provided a practical basis for rapidly expanding the feasibility and value of routine wastewater surveillance for future emerging infectious diseases.

关键词： SARS-CoV-2 wastewater surveillance wastewater-based epidemiology WWTP automated robot