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Identif ication of a hypersensitive response core peptide of HrpZ and its role in increasing grape downy mildew resistance

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Horticultural Plant Journal 2024年第5期10卷 1112-1120页

作者： Zongbao Fan Xueqiang Guan Zhichang Zhang Yushuai Sun Fei Wang Huiru Chi yuxin yao State Key Laboratory of Crop Biology Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient ProductionCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai'anShandong 271018China Shandong Academy of Grape/Shandong Technology Innovation Center of Wine Grape and Wine JinanShandong 250100China Zhichang Agricultural Science and Technology Development Co. Ltd.RizhaoShandong 276511China

Harpins play a key role in inducing disease resistance in crops,and identifying their core functional regions and establishing a system for their efficient expression would be very *** this study,large amounts of soluble fusion proteins of harpin HrpZ and its subpeptides were obtained via the optimized induction conditions(28℃ with 0.5 mmol·L^(-1) IPTG for 6 h)in Escherichia coli BL21(DE3).Hypersensitive response(HR)assays demonstrated that the C-terminal 66 aa of HrpZ(HrpZ_C_2_2)elicited a strong HR in tobacco(Nicotiana benthamiana)and grape(Flame Seedless)***,treatment with HrpZ,and particularly HrpZ_C_2_2,significantly reduced the disease incidence and severity index of field vine leaves and those inoculated with downy *** determination of the physiological parameters indicated that HrpZ,and especially HrpZ_C_2_2,improved the photosynthesis-and chlorophyll fluorescence-related parameters,enhanced the activity of defense-related enzymes,including SOD,POD,CAT and PAL,and increased the H_(2)O_(2) ***,we efficiently expressed a core peptide of HrpZ and elucidated its strong ability to elicit a HR and resistance to downy *** research provides insight into understanding the structure and function of HrpZ and will advance the application of HrpZ_C_2_2 to increase the resistance of grapevine to downy mildew.

关键词： Grapevine Harpin HrpZ Prokaryotic expression Downy mildew Hypersensitive response

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智能家居产品中的隐式交互设计研究

纺织报告 2024年第8期43卷 67-69页

作者：姚雨欣长沙理工大学湖南长沙410000

隐式人机交互作为一种新兴的交互模式,侧重于通过感知和推理减轻用户的认知和操作负担。文章首先研究了隐式交互利用日常无意识行为创造更自然、直观的交互体验,在智能家居产品使用的多元化场景下,总结了隐式交互规则,有助于提升用户对... 详细信息

隐式人机交互作为一种新兴的交互模式,侧重于通过感知和推理减轻用户的认知和操作负担。文章首先研究了隐式交互利用日常无意识行为创造更自然、直观的交互体验,在智能家居产品使用的多元化场景下,总结了隐式交互规则,有助于提升用户对智能家居产品的持续使用意愿;其次详细探讨了智能家居技术的发展、隐式交互设计的重要性并进行了案例分析;最后讨论了智能家居产品设计中定制化和动态调整的发展趋势,以期为智能家居产品用户体验提供有价值的理论参考。

关键词：智能家居产品隐式交互设计无意识动作个性化

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红肉水果种质现状及研究进展

落叶果树 2024年第2期56卷 6-11页

作者：姚玉新王菲山东农业大学园艺科学与工程学院山东泰安271018

红肉水果富含花青素,具有较高的营养和保健价值,越来越受消费者和育种者青睐。综述了目前常见的红肉水果资源,包括生产上主要栽培的红肉水果品种;总结了红肉水果中主要的花青素组分,分析了红肉和白肉果实中花青素组分差异;评价了红肉水... 详细信息

红肉水果富含花青素,具有较高的营养和保健价值,越来越受消费者和育种者青睐。综述了目前常见的红肉水果资源,包括生产上主要栽培的红肉水果品种;总结了红肉水果中主要的花青素组分,分析了红肉和白肉果实中花青素组分差异;评价了红肉水果资源作为中间材料在育种上的应用,并概述了红肉水果所提取的花青素在保健品、医药、化妆品等行业的应用;结合花青素生物合成途径,概述了果肉着色的调控机制。最后,讨论了红肉水果存在的主要问题、发展趋势及其主要用途。

关键词：红肉水果种质资源育种营养保健调控机制

电荷迁移方向调控的BiVO_(4)量子点/苝四羧酸Z型异质结与纳米金修饰在人工光合成H_(2)O_(2)中的协同作用

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Chinese Journal of Catalysis 2024年第7期62卷 277-286页

作者：梁腾李雨彤徐帅姚宇欣许荣萍边辑张紫晴井立强黑龙江大学化学化工与材料学院功能无机材料化学教育部重点实验室催化技术国际联合研究中心实验室黑龙江哈尔滨150080

利用太阳能驱动的半导体光催化技术将H_(2)O和O_(2)转化为H_(2)O_(2),是传统蒽醌工艺的可持续替代方案之一.在光催化O_(2)还原生产H_(2)O_(2)的过程中,主要涉及到O_(2)的还原和H_(2)O的氧化两个半反应.然而,单一的窄带隙半导体往往难以... 详细信息

利用太阳能驱动的半导体光催化技术将H_(2)O和O_(2)转化为H_(2)O_(2),是传统蒽醌工艺的可持续替代方案之一.在光催化O_(2)还原生产H_(2)O_(2)的过程中,主要涉及到O_(2)的还原和H_(2)O的氧化两个半反应.然而,单一的窄带隙半导体往往难以满足整体反应的热力学电位要求.为了克服这一挑战,研究人员借鉴自然光合作用的原理,提出了构建Z型异质结体系的策略.这种体系不仅能有效促进电荷的分离,从而增强光催化效率,而且能保留光生电荷较强的氧化还原能力,这对于H_(2)O_(2)的光合成至关重要.此外,O_(2)的活化对于H_(2)O_(2)光合成同样重要.然而,目前在构建Z型异质结时,对于Z型异质结的两组分自身电荷传输方向的匹配性鲜有关注.因此,构建具有电荷定向传输的Z型异质结,并精准负载O_(2)活化位点,对于提高H_(2)O_(2)的光合成效率和稳定性具有重要意义.本文以苝-3,4,9,10-四羧酸二酐(PTCDA)为前驱体,在水解的过程中,引入预制备的钒酸铋量子点(BQD),原位构建BQD/PTA Z型异质结.然后,通过低温辅助光还原策略将纳米Au精确修饰在苝四甲酸(PTA)上,制得具有定向电荷传输的BQD/PTA-Au Z型异质结.形貌表征结果表明,PTA的形态为平均厚度为3.5 nm的不规则纳米片,其表面均匀负载了直径约为7 nm的BQD纳米颗粒,而超小的Au纳米粒子(直径约为5 nm)则负载在PTA纳米片的边缘.优化后的BQD/PTA-Au异质结光催化H_(2)O_(2)产率为218.6μmol g^(-1)h^(-1),约为PTA纳米片的6.4倍;此外,在405 nm波长下,该异质结的表观量子产率为PTA的4.8倍.在模拟太阳光条件下,BQD/PTA-Au异质结的太阳能到化学能转换效率(SCC)为0.47%;且在连续5次的循环实验中表现出良好的稳定性.光物理和光化学测试结果表明,BQD/PTA Z型异质结的构建显著促进了PTA的电荷分离,Au修饰后复合体的电荷分离进一步增强.扫描开尔文探针(SKP)测试结果表明,BQD和PTA的能带结构满足Z型电荷传输路径,电子顺磁共振和原位X射线光电子能谱分析结果表明,所构建的Z型异质结由于电荷传输方向匹配,显著促进·O_(2)-自由基的形成;而后续的Au修饰则能够定向接受来自PTA的光生电子.旋转圆盘电极测试结果表明,BQD/PTA-Au的平均电子转移数最接近2,说明其遵循两电子氧还原反应(ORR)路径.结合物种捕获实验推测,·O_(2)-自由基是BQD/PTA-Au异质结光合成H_(2)O_(2)的主要活性物种.原位漫反射红外光谱测试证实,纳米Au修饰可进一步提高O_(2)在催化剂表面吸附,并加速两电子的O_(2)还原反应.综上,本文围绕着具有各向异性电荷迁移的PTA,设计构建了电荷定向迁移的Z型异质结体系,实现了高效的H_(2)O_(2)光合成,为高效Z型异质结体系设计构建提供了新思路.

关键词： Z型异质结苝四甲酸 BiVO_(4)量子点 H_(2)O_(2)光合成氧还原

融合5G网络的智能油气田安全监测预警系统应用

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计算机应用文摘 2024年第19期40卷 100-102页

作者：邓增利陈小泉匡胜姚予鑫中海石油(中国)有限公司湛江分公司广东湛江524057 中海油信息科技有限公司湛江分公司广东湛江524057

作为能源供应的重要基地,油气田的安全生产直接关系到国家能源安全和经济发展。然而,油气田的生产环境复杂多变,存在诸多安全隐患,如管道泄漏、设备故障、地质变化等。传统的管理和监测手段已难以满足现代油气田的安全生产需求。因此,融... 详细信息

作为能源供应的重要基地,油气田的安全生产直接关系到国家能源安全和经济发展。然而,油气田的生产环境复杂多变,存在诸多安全隐患,如管道泄漏、设备故障、地质变化等。传统的管理和监测手段已难以满足现代油气田的安全生产需求。因此,融合5G网络的智能油气田安全监测预警系统应运而生,为油气田的安全生产提供了强有力的技术支撑。

关键词： 5G网络管道泄漏设备故障地质变化

Melatonin Treatment Enhances the Polyphenol Content and Antioxidant Capacity of Red Wine

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Horticultural Plant Journal 2018年第4期4卷 144-150页

作者： XU Lili YUE Qianyu BIAN Feng'e ZHAI Heng yao yuxin State Key Laboratory of Crop Biology Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Huang-Huai Region Ministry of Agriculture) College of Horticulture Science and Engineering Shandong Agricultural University

Melatonin and polyphenols are strong antioxidants, and melatonin is also a potential plant growth regulator. The role of melatonin in regulating polyphenol metabolism is unclear. This study assessed the primary impacts of exogenous melatonin treatment on phenolics accumulation and antioxidant capacity of the 'Moldova' wine. It was found that two times of 100 μmol·L^(-1) melatonin treatment clearly enhanced the endogenous melatonin content of ripened berries and wine. Further experiments indicated that melatonin treatment significantly increased the contents of total phenols, flavonoids and anthocyanins in wine. Additionally, the contents of most of the 20 detected individual phenolic compounds were significantly enhanced by melatonin treatment in wine. Particularly, the content of two non-flavonoid phenolic compounds(syringic and coumaric acid) and four anthocyanin compounds [Mv-3-Glu, Mv-3-(6-Coum)Glu-5-Glu, Dp-3-(6-AC)Glu and Dp-3-(6-Coum)Glu-5-Glu]were largely increased by melatonin treatment. In contrast, only Pn-3-(6-Coum)Glu was significantly reduced. Moreover, melatonin treatment significantly enhanced the antioxidant capacity of wine indicated by DPPH and FRAP assays. In summary, melatonin treatment enhanced the polyphenol content and antioxidant capacity of wine.

关键词： grape red wine melatonin phenolics antioxidant capacity

VvMYB14 participates in melatonin-induced proanthocyanidin biosynthesis by upregulating expression of VvMYBPA1 and VvMYBPA2 in grape seeds

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Horticulture Research 2023年第2期10卷 214-226页

作者： Xiaoqian Zhang Wanyun Ma Xueqiang Guan Fei Wang Zongbao Fan Shiwei Gao yuxin yao State Key Laboratory of Crop Biology Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient ProductionCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai-AnShandong 271018China Shandong Academy of Grape/Shandong Technology Innovation Center of Wine Grape and Wine JinanShandong 250100China

This work demonstrated that melatonin increases continuously in seeds,particularly seed coats,during berry *** melatonin treatments significantly increased the proanthocyanidin(PA)content,partially through ethylene signaling,in seed ***14 expression exhibited patterns similar to melatonin accumulation over time,which was largely induced by melatonin treatment in seed coats during berry ***,VvMYB14 bound to the MBS element of the VvMYBPA1 promoter to activate ***14 overexpression largely upregulated expression of VvMYBPA1,VvMYBPA2 and VvLAR1 and increased the PA content in grape seed-derived *** increases in AtTT2 and AtBAN expression and PA content were found in VvMYB14-overexpressing Arabidopsis *** was also observed that VvMYB14 overexpression increased ethylene production and thereby induced expression of VvERF104,which bound to the ERF element of the VvMYBPA2 promoter and activated its ***,VvERF104 suppression reduced the VvMYB14 overexpression-induced increases in expression of VvMYBPA2 and VvLAR1 and PA *** experiments revealed that melatonin-induced increases in the expression of VvMYBPA1,VvMYBPA2,VvERF104 and VvLAR1 and PA accumulation were significantly reduced in VvMYB14-suppressing grape calli and ***,VvMYB14 mediates melatonin-induced PA biosynthesis by directly transactivating VvMYBPA1 expression and indirectly upregulating VvMYBPA2 expression via VvERF104.

关键词： melatonin thereby treatment

30-Year Trends in the Disease Burden, Incidence, and Prevention of Pneumoconiosis

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China CDC weekly 2023年第38期5卷 856-860页

作者： Xuezan Huang Wei Liu yuxin yao Dongming Wang Yi Sun Weihong Chen Department of Occupational and Environmental Health School of Public HealthTongji Medical CollegeHuazhong University of Science and TechnologyWuhan CityHubei ProvinceChina Key Laboratory of Environment and Health Ministry of Education and Ministry of Environmental ProtectionState Key Laboratory of Environmental Health(Incubating)School of Public HealthTongji Medical CollegeHuazhong University of Science and TechnologyWuhan CityHubei ProvinceChina Institute for Occupational Safety and Health of the German Social Accident Insurance Sankt AugustinGermany.

The global incidence of pneumoconiosis,a lung disease caused by inhaling industrial dust,has increased by 61.5%from 1990 to 2019,according to a recent *** had the highest number of cases in 2019,followed by India and the United *** all-age standard incidence rate for pneumoconiosis was 2.39 per 100,000 population in 2019,with China also having the highest ***,there was a decrease in the incidence rate in Belgium and Italy during the same *** report also highlights regional variations in the changing trend,with the largest increase in cases observed in North Africa and the Middle *** dust prevention and control measures are urgently needed to reduce workplace dust exposure and combat the rising cases of pneumoconiosis globally.

关键词： prevention lung Incidence

天文源跟踪和数据文件生成——以太阳射电流强时域变化实验为例

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物理与工程 2024年第4期34卷 141-150页

作者：安泱屹欧阳希昶姚雨欣骆彦高扬中山大学物理与天文学院天文系

本文是专题“面向天文、物理和多个工科专业的射电望远镜实验平台”上篇，内容包括望远镜基本参数和实验设计，以及天文源跟踪、数据文件生成两个实现望远镜天文观测功能的选做实验。太阳是距离我们最近的较强天文射电源，本文基于实例... 详细信息

本文是专题“面向天文、物理和多个工科专业的射电望远镜实验平台”上篇，内容包括望远镜基本参数和实验设计，以及天文源跟踪、数据文件生成两个实现望远镜天文观测功能的选做实验。太阳是距离我们最近的较强天文射电源，本文基于实例介绍了太阳射电流强时域变化实验。

关键词：天文教学射电天文观测望远镜控制频谱仪API编程太阳射电辐射