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Implantable Electrochemical Microsensors for In Vivo Monitoring of Animal Physiological Information

Nano-Micro Letters 2024年第3期16卷 183-211页

作者： Jin Zhou Shenghan Zhou Peidi Fan Xunjia Li Yibin Ying Jianfeng Ping yuxiang pan Laboratory of Agricultural Information Intelligent Sensing College of Biosystems Engineering and Food ScienceZhejiang UniversityHangzhou 310058People’s Republic of China ZJU-Hangzhou Global Scientific and Technological Innovation Center Zhejiang UniversityHangzhou 311200People’s Republic of China

In vivo monitoring of animal physiological information plays a crucial role in promptly alerting humans to potential diseases in animals and aiding in the exploration of mechanisms underlying human diseases.Currently,implantable electrochemical microsensors have emerged as a prominent area of research.These microsensors not only fulfill the technical requirements for monitoring animal physiological information but also offer an ideal platform for integration.They have been extensively studied for their ability to monitor animal physiological information in a minimally invasive manner,characterized by their bloodless,painless features,and exceptional performance.The development of implantable electrochemical microsensors for in vivo monitoring of animal physiological information has witnessed significant scientific and technological advancements through dedicated efforts.This review commenced with a comprehensive discussion of the construction of microsensors,including the materials utilized and the methods employed for fabrication.Following this,we proceeded to explore the various implantation technologies employed for electrochemical microsensors.In addition,a comprehensive overview was provided of the various applications of implantable electrochemical microsensors,specifically in the monitoring of diseases and the investigation of disease mechanisms.Lastly,a concise conclusion was conducted on the recent advancements and significant obstacles pertaining to the practical implementation of implantable electrochemical microsensors.

关键词： Electrochemical microsensors Implantable sensors In vivo monitoring Animal physiological information

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土地经营权流转期限的分层调整制度设计与战略构想——基于分配正义视角

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现代经济探讨 2024年第4期 125-132页

作者：潘雨祥西南政法大学民商法学院重庆401120

土地经营权被先后纳入土地承包法和民法典,搭建起农地三权分置格局,但其权利构建尚未完成。土地经营权制度构建事关农地权利分配正义,维护承包权主体利益、满足经营权主体需求、促进农地资源优化利用是其基本要求,土地经营权流转期限正... 详细信息

土地经营权被先后纳入土地承包法和民法典,搭建起农地三权分置格局,但其权利构建尚未完成。土地经营权制度构建事关农地权利分配正义,维护承包权主体利益、满足经营权主体需求、促进农地资源优化利用是其基本要求,土地经营权流转期限正是这些基本要求的集中体现。因此,流转期限的分层调整负载了土地经营权制度构建的现实基础、价值依据和技术路径。根植于农地流转实践,因循中国物权和债权制度现实,可将土地经营权流转期限分层为短期、中长期以及长期,赋予不同期限的土地经营权以不同性质。并在此基础上通过确保土地收回权落地等保障性措施,促进土地经营权的中长期流转,为土地经营权制度构建奠定符合中国实际的法理和制度基础。

关键词：分配正义土地经营权流转期限分层调整中长期流转

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3D microgroove electrical impedance sensing to examine 3D cell cultures for antineoplastic drug assessment

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Microsystems & Nanoengineering 2020年第1期6卷 1199-1208页

作者： yuxiang pan Deming Jiang Chenlei Gu Yong Qiu Hao Wan Ping Wang Biosensor National Special Laboratory Key Laboratory for Biomedical Engineering of Education MinistryDepartment of Biomedical EngineeringZhejiang UniversityHangzhou 310027China State Key Laboratory of Transducer Technology Chinese Academy of SciencesShanghai 200050China

In recent decades,three-dimensional(3D)cancer cell models have attracted increasing interest in the field of drug screening due to their significant advantages in more accurate simulations of heterogeneous tumor behavior in vivo compared to two-dimensional models.Furthermore,drug sensitivity testing based on 3D cancer cell models can provide more reliable in vivo efficacy prediction.The gold standard fluorescence staining is hard to achieve real-time and label-free viability monitoring in 3D cancer cell models.In this study,a microgroove impedance sensor(MGIS)was specially developed for the dynamic and noninvasive monitoring of 3D cell viability.3D cancer cells were trapped in microgrooves with gold electrodes on opposite walls for in situ impedance measurement.The change in the number of live cells caused inversely proportional changes to the impedance magnitude of the entire cell/Matrigel construct and reflected the proliferation and apoptosis of the 3D cells.It was confirmed that the 3D cell viability detected by the MGIS was highly consistent with the standard live/dead staining by confocal microscope characterization.Furthermore,the accuracy of the MGIS was validated quantitatively using a 3D lung cancer model and sophisticated drug sensitivity testing.In addition,the parameters of the MGIS in the measurement experiments were optimized in detail using simulations and experimental validation.The results demonstrated that the MGIS coupled with 3D cell culture would be a promising platform to improve the efficiency and accuracy of cell-based anticancer drug screening in vitro.

关键词： impedance walls electrical

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Covalently grafting first-generation PAMAM dendrimers onto MXenes with self-adsorbed AuNPs for use as a functional nanoplatform for highly sensitive electrochemical biosensing of cTnT

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Microsystems & Nanoengineering 2022年第2期8卷 119-133页

作者： Xin Liu Yong Qiu Deming Jiang Fengheng Li Ying Gan Yuxuan Zhu yuxiang pan Hao Wan Ping Wang Zhejiang University 310027 HangzhouChina Cancer Centre Zhejiang University310058 HangzhouZhejiangChina State Key Laboratory of Transducer Technology Chinese Academy of Sciences200050 ShanghaiChina Binjiang Institute of Zhejiang University 310053 HangzhouChina. School of Biomedical Engineering Tianjin Medical University300070 TianjinChina Research Center of Smart Sensing ZhejiangLab310027 HangzhouChina

2D MXene-Ti3C2Tx has demonstrated promising application prospects in various fields;however,it fails to function properly in biosensor setups due to restacking and anodic oxidation problems.To expand beyond these existing limitations,an effective strategy to for modifying the MXene by covalently grafting first-generation poly(amidoamine)dendrimers onto an MXene in situ(MXene@PAMAM)was reported herein.When used as a conjugated template,the MXene not only preserved the high conductivity but also conferred a specific 2D architecture and large specific surface areas for anchoring PAMAM.The PAMAM,an efficient spacer and stabilizer,simultaneously suppressed the substantial restacking and oxidation of the MXene,which endowed this hybrid with improved electrochemical performance compared to that of the bare MXene in terms of favorable conductivity and stability under anodic potential.Moreover,the massive amino terminals of PAMAM offer abundant active sites for adsorbing Au nanoparticles(AuNPs).The resulting 3D hierarchical nanoarchitecture,AuNPs/MXene@PAMAM,had advanced structural merits that led to its superior electrochemical performance in biosensing.As a proof of concept,this MXene@PAMAM-based nanobiosensing platform was applied to develop an immunosensor for detecting human cardiac troponin T(cTnT).A fast,sensitive,and highly selective response toward the target in the presence of a[Fe(CN)6]^(3-/4-)redox marker was realized,ensuring a wide detection of 0.1-1000 ng/mL with an LOD of 0.069 ng/mL.The sensor's signal only decreased by 4.38%after 3 weeks,demonstrating that it exhibited satisfactory stability and better results than previously reported MXene-based biosensors.This work has potential applicability in the bioanalysis of cTnT and other biomarkers and paves a new path for fabricating high-performance MXenes for biomedical applications and electrochemical engineering.

关键词： electrochemical anodic properly

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玉米须活性成分的提取分离及其生物活性研究

玉米须活性成分的提取分离及其生物活性研究

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作者： yuxiang pan 天津大学

学位级别：硕士

玉米须,又名玉蜀黍须,是禾本科植物玉蜀黍Zea mays L.的花柱和柱头。全国大部分地区均产,夏、秋果实成孰时收集,除去杂质,鲜用或晒干生用。玉米在我国大江南北广为栽培,因此玉米须资源十分丰富。玉米须是我国一种传统的中草药,被1985版... 详细信息

玉米须,又名玉蜀黍须,是禾本科植物玉蜀黍Zea mays L.的花柱和柱头。全国大部分地区均产,夏、秋果实成孰时收集,除去杂质,鲜用或晒干生用。玉米在我国大江南北广为栽培,因此玉米须资源十分丰富。玉米须是我国一种传统的中草药,被1985版(一部)的《中华人民共和国卫生部药材标准》收录为常用药材品种,作为我国的传统中药材,民间常用于治疗高血压和糖尿病。玉米须最早的入药记载是在《滇南本草》,谓其性微温,味甜,入阳明胃经,宽肠下气,治疗妇人乳汁不通、乳结红肿、睡卧压着、小儿吹着、头痛体困、怕冷发热等。医家认为玉米须味甘与淡,性平。不仅入阳明胃经,而且还归肝、胆、肾经,功能清肝利胆、利水消肿。现代药理研究也证实玉米须具有多种药理活性,如降血糖、降血压、降血脂、抗肿瘤、保肝、清热利胆、免疫调节等。玉米须化学成分复杂多样,主要含有黄酮类化合物、甾体类化合物、多糖、皂苷类化合物、有机酸、生物碱、氨基酸、维生素等。研究表明玉米须中的主要活性成分包括多糖、多酚类、黄酮类、皂苷等。为了明确玉米须中的化学成分及其生物活性,本文对玉米须的化学成分与其抗氧化、降血糖活性进行了系统的研究。银纳米颗粒在电学、光学、生物医学和催化等领域展现出了十分优异的性能,尤其在生物医学方面,具有广阔的应用空间。银纳米颗粒由于其生物兼容性、表面易修饰、小尺寸效应和极大的比表面积等特性,容易与病原微生物接触,从而发挥更大的生物效应,因此具有抗菌范围广和持续杀菌时间长的优点。然而,在对银纳米颗粒的利用过程中,人们逐渐发现银纳米颗粒的表面必须做到无化学试剂残留方可对其进行进一步的生物应用,从而达到预期的效果,而其中银纳米颗粒的品质直接取决于制备方法。生物还原法是近年来兴起的一种新的银纳米颗粒的合成方法,分为微生物法和植物取液法,生物还原法条件温和、安全、环保、能耗低。其中,植物取液法相比于微生物法更具优势,它不需要复杂的过程,如微生物细胞培养和多个净化步骤。植物取液的来源广泛,取便利,用植物取液合成的银纳米颗粒无毒、无污染,更利于银纳米颗粒在医学上的应用。目前,采用的植物主要包括西袖皮、红枣、橙子和称猴桃等。尚未见用玉米须取液来合成得到银纳米颗粒的报道。玉米须在中国的分布广泛,价格低廉,无毒,便于储存,不会造成环境污染,并且有药用价值,以玉米须取液来制备银纳米颗粒具有合成效果好和绿色安全无毒的优势。本文首先对玉米须中的多糖进行了结构表征和抗氧化、降血糖等生物活性的研究。采用80%乙醇取干燥玉米须粉末得到玉米须醇物和滤渣,滤渣再采用水醇沉法得到玉米须粗多糖,Savage法除去蛋白,反复醇沉除去小分子,得到的粗多糖再经过超滤得到分子量小于30000、30000-80000、大于80000道尔顿的多糖,通过体外抗氧化实验(DPPH自由基清除实验、铁还原力实验)和α淀粉酶抑制实验发现,分子量为30000-80000道尔顿间的多糖(PCS2)活性最好,故对其进行结构表征及进一步活性研究。采用GPC分子排阻色谱法确定PCS2的平均分子量为45500 Da左右,FT-IR红外数据表明了多糖的特征峰,扫电镜观察得到了多糖多孔且光滑的表面结构,气相色谱法确定了多糖的单糖组成及比例为半乳糖:甘露糖:葡萄糖:木糖:阿拉伯糖:鼠李糖=1.00:0.67:0.63:0.42:0.16:0.08。对于PCS2的降血糖活性研究,采用了高糖高脂饲料配合链脲佐菌素注射诱导的二型糖尿病小鼠模型,实验结果显示PCS2可以显著改善糖尿病小鼠的饮食摄水,显著降低了糖尿病小鼠的空腹血糖值。葡萄糖耐受实验结果表明PCS2显著改善了小鼠的葡萄糖耐受。通过测定血清指标发现游离脂肪酸、低密度脂肪酸、糖化血清蛋白等的水平较糖尿病小鼠阴性对照组都有了显著降低,同时体内抗氧化酶如谷胱甘肽过氧化物酶、过氧化氢酶、超氧化物歧化酶的活性都有了显著的增高。这些结果说明了PCS2作为一种天然的功能食品有用于治疗糖尿病的潜力。其次是对玉米须醇溶性成分进行分级(石油醚,乙酸乙酯,正定醇)萃取,对比不同极性部分的化学成分和生物活性,再采用活性追踪的方法对正丁醇层进行分离纯化。首先为了明确玉米须的活性部位,本文首先对比研究了玉米须不同极性部位(石油醚,乙酸乙酯,正定醇)的化学成分与抗氧化活性。实验结果表明玉米须石油醚层具有最高的总甾醇含量但是体外抗氧化活性和消化酶抑制活性(α-淀粉酶抑制实验、α-葡萄糖苷酶抑制实验)最低,而乙酸乙酯层和正丁醇层的总多酚、总黄酮含量及体外抗氧化活性(DPPH自由基清除实验,铁还原力实验)、消化酶抑制活性(α-淀粉酶抑制实验、α-葡萄糖苷酶抑制实验)相对较高,这说明了多酚和黄酮类物质可能是玉米须中起到抗氧化和消化酶抑制作用的主要成分。同样采用高糖高脂饲料配合链脲佐菌素注射诱导的二型糖尿病小鼠模型对正丁醇层萃取物进行体内降血糖活性实验,结果显示正丁醇层萃取物可以显著降低血糖水平,调节胰岛素分泌及葡萄糖耐受。正丁醇层萃取

关键词：玉米须多糖正丁醇层萃取物抗糖尿病作用银纳米颗粒

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基于LASSO回归探讨多种生物标志物预测急性冠脉综合征病人PCI术后造影剂肾病的价值

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中西医结合心脑血管病杂志 2024年第12期22卷 2246-2251页

作者：杨珊珊潘宇翔郑婉王政海南医学院第一附属医院海口570102 海南医学院第二附属医院海口570102

目的:基于最小绝对收缩与选择算法(LASSO)回归探讨多种生物标志物预测急性冠脉综合征病人经皮冠状动脉介入治疗(PCI)术后造影剂肾病的价值。方法:选取2020年1月-2022年6月在海南医学院第一附属医院就诊的119例急性冠脉综合征病人作为研... 详细信息

目的:基于最小绝对收缩与选择算法(LASSO)回归探讨多种生物标志物预测急性冠脉综合征病人经皮冠状动脉介入治疗(PCI)术后造影剂肾病的价值。方法:选取2020年1月-2022年6月在海南医学院第一附属医院就诊的119例急性冠脉综合征病人作为研究对象,记录病人一般情况、血液生化指标、炎性因子、生物标志物及PCI术后3 d造影剂肾病发生情况,以病人PCI术后3 d是否发生造影剂肾病为因变量,运用10折交叉验证LASSO回归进行特征性变量筛选,以特征性变量作为自变量代入多因素Logistic回归模型获得急性冠脉综合征病人PCI术后发生造影剂肾病的独立预测因素,重点分析生物标志物与病人PCI术后发生造影剂肾病的关系,并基于独立预测因素构建联合预测模型和列线图模型,绘制校准曲线以验证列线图模型的预测效能。结果:10折交叉验证LASSO回归筛选出最具泛化能力的4个特征性变量糖尿病、尿肾损伤分子-1(KIM-1)、尿中性粒细胞明胶酶相关载脂蛋白(NGAL)以及尿胱抑素C(CysC),其对应的LASSO回归系数分别为0.436,0.624,0.916及2.745,多因素Logistic回归分析调整和校正混杂因素后,糖尿病、尿KIM-1、尿NGAL以及尿CysC为急性冠脉综合征病人PCI术后发生造影剂肾病的独立预测因素(P<0.05),基于急性冠脉综合征病人PCI术后发生造影剂肾病的独立预测因素糖尿病、尿KIM-1、尿NGAL以及尿CysC构建列线图模型,P=1/(1+e^(-x)),X=-2.345+0.824×尿CysC+0.565×糖尿病+0.685×尿NGAL+0.634×尿KIM-1,列线图模型的校准曲线显示,急性冠脉综合征病人PCI术后发生造影剂肾病风险的预测值与实际观测值符合度良好。结论:糖尿病、尿KIM-1、尿NGAL以及尿CysC为急性冠脉综合征病人PCI术后发生造影剂肾病的独立预测因素,基于独立预测因素建立的列线图模型具有较高的预测价值。

关键词：急性冠脉综合征造影剂肾病最小绝对收缩与选择算法回归,LASSO回归生物标志物预测价值

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Synthesis of mordenite by solvent-free method and its application in the dimethyl ether carbonylation reaction

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Chinese Journal of Chemical Engineering 2024年第2期66卷 216-223页

作者： Yinghui Liu Shaoduo Liu Xiaosheng Wang Hongjing Wang Ranjia Li Changchun Yu Chunming Xu yuxiang Liu Zhengqiu Xie Yongqiang Wang pan Tang State Key Laboratory of Heavy Oil Processing College of New Energy and MaterialsChina University of Petroleum-BeijingBeijing 102249China Synfuels China Technology Co. LtdBeijing 101400China College of Chemical Engineering Qingdao University of Science&TechnologyQingdao 266042China Yueyang Jucheng Chemical Co. LtdYueyang 414009China

Mordenite with different Si/Al ratios were synthesized by solvent-free method and used for dimethyl ether(DME)carbonylation reaction.The influence of Si/Al ratio in the feedstock on the structure,porosity and acid sites were systematically investigated.The characterization results showed that with the increase of Si/Al ratio in the feedstock,part of silicon species fail to enter the skeleton and the specific surface area and pore volume of the samples decreased.The amount of weak acid and medium strong acid decreased alongside with the increasing Si/Al ratio,and the amount of strong acid slightly increased.The Al atoms preferentially enter the strong acid sites in the 8 member ring(MR)channel during the crystallization process.The high Si/Al ratio sample had more acid sites located in the 8 MR channel,leading to more active sites for carbonylation reaction and higher catalytic performance.Appropriately increasing the Si/Al ratio was beneficial for the improvement of carbonylation reaction activity over the mordenite(MOR)catalyst.

关键词： Carbonylation Dimethyl ether Solvent-free Zeolite Synthesis Catalysis

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A Golden2-like transcription factor, BnGLK1a, improves chloroplast development, photosynthesis, and seed weight in rapeseed

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Journal of Integrative Agriculture 2024年第5期23卷 1481-1493页

作者： Qianwei zhang Yuanyi Mao Zikun Zhao Xin Hu Ran Hu Nengwen Yin Xue Sun Fujun Sun Si Chen yuxiang jiang Liezhao Liu Kun Lu Jiana Li Yu pan Integrative Science Center of Germplasm Creation in Western China(Chongqing)Science City Southwest UniversityChongqing 400715China Academy of Agricultural Sciences Southwest UniversityChongqing 400715China Engineering Research Center of South Upland Agriculture Ministry of EducationChongqing 400715China Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education/College of Horticulture and Landscape ArchitectureSouthwest UniversityChongqing 400715China

Enhancing photosynthetic efficiency is a major goal for improving crop yields under agricultural field conditions and is associated with chloroplast biosynthesis and development.In this study,we demonstrate that Golden2-like 1a(BnGLK1a)plays an important role in regulating chloroplast development and photosynthetic efficiency.Overexpressing BnGLK1a resulted in significant increases in chlorophyll content,the number of thylakoid membrane layers and photosynthetic efficiency in Brassica napus,while knocking down BnGLK1a transcript levels through RNA interference(RNAi)had the opposite effects.A yeast two-hybrid screen revealed that BnGLK1a interacts with the abscisic acid receptor PYRABACTIN RESISTANCE 1-LIKE 1-2(BnPYL1-2)and CONSTITUTIVE PHOTOMORPHOGENIC 9 SIGNALOSOME 5A subunit(BnCSN5A),which play essential roles in regulating chloroplast development and photosynthesis.Consistent with this,BnGLK1a-RNAi lines of B.napus display hypersensitivity to the abscisic acid(ABA)response.Importantly,overexpression of BnGLK1a resulted in a 10%increase in thousand-seed weight,whereas seeds from BnGLK1a-RNAi lines were 16%lighter than wild type.We propose that BnGLK1a could be a potential target in breeding for improving rapeseed productivity.Our results not only provide insights into the mechanisms of BnGLK1a function,but also offer a potential approach for improving the productivity of Brassica species.

关键词： Brassica napus BnGLK1a chloroplast development photosynthetic efficiency yield

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Enabling an Inorganic-Rich Interface via Cationic Surfactant for High-Performance Lithium Metal Batteries

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Nano-Micro Letters 2024年第8期16卷 1-17页

作者： Zejun Sun Jinlin Yang Hongfei Xu Chonglai Jiang yuxiang Niu Xu Lian Yuan Liu Ruiqi Su Dayu Liu Yu Long Meng Wang Jingyu Mao Haotian Yang Baihua Cui Yukun Xiao Ganwen Chen Qi Zhang Zhenxiang Xing Jisheng pan Gang Wu Wei Chen Department of Chemistry National University of Singapore3 Science Drive 3Singapore 117543Singapore Agency for Science Technology and Research(A*STAR)Institute of High-Performance Computing1 Fusionopolis Way#16‑16 ConnexisSingapore 138632Singapore Department of Physics National University of Singapore2 Science Drive 3Singapore 117542Singapore Joint School of National University of Singapore and Tianjin University International Campus of Tianjin UniversityBinhai New CityFuzhou 350207People’s Republic of China Agency for Science Technologyand Research(A*STAR)Institute of Materials Research and EngineeringInnovis2 Fusionopolis Way#08‑03Singapore 138634Singapore

An anion-rich electric double layer(EDL)region is favorable for fabricating an inorganic-rich solid-electrolyte interphase(SEI)towards stable lithium metal anode in ester electrolyte.Herein,cetyltrimethylammonium bromide(CTAB),a cationic surfactant,is adopted to draw more anions into EDL by ionic interactions that shield the repelling force on anions during lithium plating.In situ electrochemical surface-enhanced Raman spectroscopy results combined with molecular dynamics simulations validate the enrichment of NO_(3)^(−)/FSI−anions in the EDL region due to the positively charged CTA^(+).In-depth analysis of SEI structure by X-ray photoelectron spectroscopy and time-of-flight secondary ion mass spectrometry results confirmed the formation of the inorganic-rich SEI,which helps improve the kinetics of Li^(+)transfer,lower the charge transfer activation energy,and homogenize Li deposition.As a result,the Li||Li symmetric cell in the designed electrolyte displays a prolongated cycling time from 500 to 1300 h compared to that in the blank electrolyte at 0.5 mA cm^(-2) with a capacity of 1 mAh cm^(-2).Moreover,Li||LiFePO_(4) and Li||LiCoO_(2) with a high cathode mass loading of>10 mg cm^(-2) can be stably cycled over 180 cycles.

关键词： Cationic surfactant Lithium nitrate additive Solid-electrolyte interphase Electric double layer Lithium metal batteries

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基于三维激光点云的公路曲线桥梁三维形貌与变形测量

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东南大学学报:自然科学版 2023年第5期53卷 756-764页

作者：王浩琛冯东明吴刚潘宇翔卢静东南大学混凝土及预应力混凝土结构教育部重点实验室南京211189 东南大学智慧建造与运维国家地方联合工程研究中心南京211189 南京奥达升智能科技有限公司南京211189

针对公路曲线桥梁检测问题,提出了一种基于三维激光扫描技术的三维形貌与变形提取方法.将原始点云数据进行包含降采样和去噪的预处理,自动提取桥梁轴线来计算桥梁里程并定位变形测点,通过对构件点云坐标系的转换实现不同构件空间几何信... 详细信息

针对公路曲线桥梁检测问题,提出了一种基于三维激光扫描技术的三维形貌与变形提取方法.将原始点云数据进行包含降采样和去噪的预处理,自动提取桥梁轴线来计算桥梁里程并定位变形测点,通过对构件点云坐标系的转换实现不同构件空间几何信息的批量提取.完成了包含一座连续梁高架桥及其匝道桥在内、总长约3000 m的桥梁结构的测量.结果表明,测量对象的主梁高程变化值在0~5 mm间均匀分布,桥面线形平顺,无明显变形,桥墩无明显扭转,但偏移较为普遍,最大值为19.5 mm,需跟踪观测.所提方法具有较高的可靠性与实际可行性,能够满足公路曲线桥梁三维形貌与变形指标全面测量的需要.

关键词：桥梁检测变形测量三维激光扫描曲线桥

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