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MEK inhibitors for the treatment of extracranial arteriovenous malformations

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Chinese Journal of Plastic and Reconstructive Surgery 2023年第3期5卷 141-144页

作者： Zi’an Xu Jingwei Zhou yuxi chen Xi Yang chen Hua Yunbo Jin Xiaoxi Lin Department of Plastic and Reconstructive Surgery Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200011China

Arteriovenous malformations(AVMs)are serious congenital vascular anomalies in which the arteries connect directly with veins without *** condition will continue to worsen without proper intervention and cause ulcers,repeated hemorrhages,and even cardiac *** treatment options for AVMs include surgery and interventional treatment;however,they are associated with high risk and recurrence *** studies revealed that excessive activation of the Ras/MAPK pathway can induce the formation and development of peripheral AVM,whereas MEK inhibitors can effectively control nidus progression,making them a potential novel treatment for *** review provides an up-to-date overview of correlated laboratory and clinical research to provide information for further research and clinical practice.

关键词： Arteriovenous malformation MEK1 MEKi Trametinib MAPK

CRISPR-assisted transcription activation by phaseseparation proteins

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Protein & Cell 2023年第12期14卷 874-887页

作者： Jiaqi Liu yuxi chen Baoting Nong Xiao Luo Kaixin Cui Zhan Li Pengfei Zhang Wenqiong Tan Yue Yang Wenbin Ma Puping Liang Zhou Songyang State Key Laboratory of Biocontrol MOE Key Laboratory of Gene Function and Regulation and Guangzhou Key Laboratory of Healthy Aging ResearchSchool of Life SciencesSun Yat-senUniversityGuangzhou510275China Sun Yat-sen Memorial Hospital Sun Yat-sen UniversityGuangzhou 510275China Lumiere Therapeutics Suzhou215000China

The clustered regularly interspaced short palindromic repeats(CRISPR)-Cas9 system has been widely used for genome engineering and transcriptional regulation in many different *** CRISPR-activation(CRISPRa)platforms often require multiple components because of inefficient transcriptional ***,we fused different phase-separation proteins to dCas9-VPR(dCas9-VP64-P65-RTA)and observed robust increases in transcriptional activation ***,human NUP98(nucleoporin 98)and FUS(fused in sarcoma)IDR domains were best at enhancing dCas9-VPR activity,with dCas9-VPR-FUS IDR(VPRF)outperforming the other CRISPRa systems tested in this study in both activation efficiency and system ***9-VPRF overcomes the target strand bias and widens gRNA designing windows without affecting the off-target effect of *** findings demonstrate the feasibility of using phase-separation proteins to assist in the regulation of gene expression and support the broad appeal of the dCas9-VPRF system in basic and clinical applications.

关键词： CRISPR transcriptional activation phase-separation proteins

Poly(lactic acid)/Poly(butylene adipate-co-terephthalate)films with simultaneous high oxygen barrier and fast degradation properties

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Green Energy & Environment 2025年第1期10卷 1-10页

作者： Mengjing Yang yuxi Mao Penghui Zhang Jie Li Zeming Tong Zhenguo Liu Yanhui chen Institute of Flexible Electronics Northwestern Polytechnical UniversityXi'an710072China Key Laboratory of Flexible Electronics of Zhejiang Province Ningbo Institute of Northwestern Polytechnical University218 Qingyi RoadNingbo315103China School of Chemistry and Chemical Engineering Shaanxi Key Laboratory of Macromolecular Science and TechnologyKey Laboratory of Special Functional and Smart Polymer Materials of Ministry of Industry and Information TechnologyNorthwestern Polytechnical UniversityXi'an 710072China

Although poly(lactic acid)(PLA)is a good environmentally-friendly bio-degradable polymer which is used to substitute traditional petrochemical-based polymer packaging films,the barrier properties of PLA films are still insufficient for high-barrier packaging *** this study,oxygen scavenger hydroxyl-terminated polybutadiene(HTPB)and cobalt salt catalyst were incorporated into the PLA/poly(butylene adipate-co-terephthalate)(PLA/PBAT),followed by melting extrusion and three-layer co-extrusion blown film process to prepare the composite *** oxygen permeability coefficient of the composite film combined with 6 wt%oxygen scavenger and 0.4 wt%catalyst was decreased significantly from 377.00 cc·mil·m^(-2)·day^(-1)·0.1 MPa^(-1) to 0.98 cc·mil·m^(-2)·day^(-1)·0.1 MPa^(-1),showing a remarkable enhancement of 384.69 times compared with the PLA/PBAT composite ***,the degradation behavior of the composite film was also accelerated,exhibiting a mass loss of nearly 60%of the original mass after seven days of degradation in an alkaline environment,whereas PLA/PBAT composite film only showed a mass loss of 32%.This work has successfully prepared PLA/PBAT composite films with simultaneously improved oxygen barrier property and degradation behavior,which has great potential for high-demanding green chemistry packaging industries,including food,agricultural,and military packaging.

关键词： Barrier property Oxygen scavenging Blow molding Eco-packaging Degradable composite film

改进CBBA算法的风电场无人机群巡检任务分配

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太阳能学报 2024年第10期45卷 554-565页

作者：焦嵩鸣陈雨溪华北电力大学自动化系保定071003

提出一种基于改进的共识捆绑算法(CBBA)的多无人机风电场巡检任务分配方法。首先,根据风电机组基础信息,采用K-均值算法确定无人机数量和安放位置,并构建风电机组巡检价值评估函数,更为合理地评估各风电机组的巡检收益;然后,针对无人机... 详细信息

提出一种基于改进的共识捆绑算法(CBBA)的多无人机风电场巡检任务分配方法。首先,根据风电机组基础信息,采用K-均值算法确定无人机数量和安放位置,并构建风电机组巡检价值评估函数,更为合理地评估各风电机组的巡检收益;然后,针对无人机的归巢返程问题,提出一种基于CBBA算法的任务包双向构建策略;最后,为克服传统CBBA算法中存在的因个体“贪婪”导致的整体效益下降问题,设计一种基于类别的奖励制度,以提高任务分配的合理性。试验表明,所提方法可根据风速、风向的变化制定相应的巡检任务分配方案,可为无人机群自主巡检风电场的任务分配提供可参考的方案。

关键词：风电机组巡检无人机共识捆绑 CBBA算法

A novel undifferentiated spermatogonia-speci?c surface protein 1(USSP1) in neonatal mice

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Science Bulletin 2019年第8期64卷 524-533页

作者： Zhuoheng Lin Puping Liang Zhaokai Yao yuxi chen Xiya Zhang Rui Huang Zhen Zhang Minyan Li Wenbin Ma Haiyan Zheng Shanbo Cao Guang Shi Xiaoyang Zhao Zhou Songyang Junjiu Huang Key Laboratory of Gene Function and Regulation of the Ministry of Education State Key Laboratory of Biocontrol School of Life Sciences Sun Yat-sen University Key Laboratory of Reproductive Medicine of Guangdong Province School of Life Sciences and the First Affiliated Hospital Sun Yat-sen University Department of Developmental Biology School of Basic Medical Science Southern Medical University Key Laboratory of Reproductive Medicine of Guangdong Province Third Affiliated Hospital of Guangzhou Medical University State Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center Sun Yat-sen University

Mammalian spermatogenesis is maintained by a rare population of spermatogonial stem cells(SSCs),which are important for male fertility. SSCs remain a subset of undifferentiated spermatogonia, which can be isolated by a combination of surface markers. Specific markers to identify and isolate undifferentiated spermatogonia are lacking. Ussp1, a transcript previously annotated as long noncoding RNA(RIKEN cDNA 4933427D06, Gene ID: 232217), virtually encodes a membrane protein, USSP1, in a highly testisspecific manner in mouse. We demonstrate its expression on the membrane of undifferentiated spermatogonia by a homemade polyclonal rabbit antibody against the protein. In vivo, USSP1^+ clusters consist mainly of As, Apr(GFRa1^+) and Aal(PLZF^+) cells. USSP1^+ cells exhibit enrichment of undifferentiated spermatogonia, as shown by increased expression of SSC self-renewal molecular markers and the potential to form SSC clones in vitro and in vivo. However, Ussp1 knockout did not affect the number of SSCs or spermatogenesis in mice. Thy1^+ cells from Ussp1 null mice did not show any defect in the SSC colony formation capacity, indicating that USSP1 is not essential for SSC self-renewal. Our data demonstrate that Ussp1 is specifically expressed in undifferentiated murine spermatogonia, indicating the potential to sort undifferentiated spermatogonia with USSP1 antibodies. Ussp1 might be a good maker for SSC enrichment in neonatal mice.

关键词： 4933427D06RIK USSP1 Spermatogenesis Spermatogonial stem cells CRISPR/Cas9

Intelligent Design of Computer Bridge

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Intelligent Design of Computer Bridge

Effective gene editing by high-fidelity base editor 2 in mouse zygotes

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作者： Jize Wang Hongkun Qiu yuxi chen Yajie Wang College of Computer Science Shenyang Aerospace University Engineering Training Center Shenyang Aerospace University School of Aero-engine Shenyang Aerospace University

Computer games are divided into complete information games and incomplete information games. Bridge is a type of incomplete information games. Due to the limited information that each player knows, the existing methods for solving complete information games cannot be directly applied to the bridge. Therefore, improving the ability of computer bridge agents is of great significance to the research in the field of artificial intelligence. This paper proposes a new bridge game system design structure and applies an improved Monte Carlo sampling algorithm to the computer bridge. Aiming at the problem that the state space information in the incomplete information game is huge and the optimal card can not be found in a reasonable time, a filtering mechanism is proposed to filter the sampling. And, a bidding module is designed, which can handle the stages of response and call when bidding. The experiment results show that the performance of the computer bridge system in this paper has certain reference value for incomplete information games.

关键词： Bridge Computer Games Incomplete Infomation Monte Carlo

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Protein & Cell 2017年第8期8卷 601-611页

作者： Puping Liang Hongwei Sun Ying Sun Xiya Zhang Xiaowei Xie Jinran Zhang zhen Zhang yuxi chen chenhui Ding Yuanyan Xiong Wenbin Ma Dan Liu Junjiu Huang Zhou Songyang Key Laboratory of Gene Engineering of the Ministry of Education Guangzhou Key Laboratory of Healthy Aging Researchand State Key Laboratory of Biocontrol SYSU-BCM Joint Research Center School of Life Sciences Sun Yat-sen UniversityGuangzhou 510275 China State Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou 510275 China Key Laboratory of Reproductive Medicine of Guangdong Province School of Life Sciences and the First Affiliated HospitalSun Yat-sen University Guangzhou 510275 China Guangzhou Magigen Biotechnology Co.Ltd Guangzhou 510320 China 5 Vernaand Marrs Mclean Department of Biochemistry and Molecular Biology Baylor College of Medicine One Baylor Plaza Houston TX 77030 USA

Targeted point mutagenesis through homologous recombination has been widely used in genetic studies and holds considerable promise for repairing disease- causing mutations in patients. However, problems such as mosaicism and low mutagenesis efficiency continue to pose challenges to clinical applicaUon of such approaches. Recently, a base editor （BE） system built on cytidine （C） deaminase and CRISPR/Cas9 technology was developed as an alternative method for targeted point mutagenesis in plant, yeast, and human cells. Base editors convert C in the deamination window to thymidine （T） efficiently, however, it remains unclear whether targeted base editing in mouse embryos is feasible. In this report, we generated a modified high- fidelity version of base editor 2 （HF2-BE2）, and investigated its base editing efficacy in mouse embryos. We found that HF2-BE2 could convert C to T efficiently, with up to 100% biallelic mutation efficiency in mouse embryos. Unlike BE3, HF2-BE2 could convert C to T on both the target and non-target strand, expanding the editing scope of base editors. Surprisingly, we found HF2-BE2 could also deaminate C that was proximal to the gRNA-binding region. Taken together, our work demonstrates the feasibility of generating point mutations in mouse by base editing, and underscores the need to carefully optimize base editing systems in order to eliminate proximal-site deamination.

关键词： base editor high-fidelity mouse embryos,proximal-site deamination whole-genome sequencing

CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes

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Protein & Cell 2015年第5期6卷 363-372页

作者： Puping Liang Yanwen Xu Xiya Zhang chenhui Ding Rui Huang Zhen Zhang Jie Lv Xiaowei Xie yuxi chen Yujing Li Ying Sun Yaofu Bai Zhou Songyang Wenbin Ma Canquan Zhou Junjiu Huang Guangdong Province Key Laboratory of Reproductive Medicine the First Affiliated Hospital and Key Laboratory of GeneEngineering of the Ministry of Education School of Life Sciences Sun Yat-sen University Guangzhou 510275 China

Genome editing tools such as the clustered regularly interspaced short palindromic repeat （CRISPR）-associated system （Cas） have been widely used to modify genes in model systems including animal zygotes and human cells, and hold tremendous promise for both basic research and clinical applications. To date, a serious knowledge gap remains in our understanding of DNA repair mechanisms in human early embryos, and in the efficiency and potential off-target effects of using technologies such as CRISPR/Cas9 in human pre-implantation embryos. In this report, we used tripronuclear （3PN） zygotes to further investigate CRISPR/Cas9-mediated gene editing in human cells. We found that CRISPR/Cas9 could effectively cleave the endogenous β-globin gene （HBB）. However, the efficiency of homologous recombination directed repair （HDR） of HBB was low and the edited embryos were mosaic. Off-target cleavage was also apparent in these 3PN zygotes as revealed by the T7E1 assay and whole-exome sequencing. Furthermore, the endogenous delta-globin gene （HBD）, which is homologous to HBB, competed with exogenous donor oligos to act as the repair template, leading to untoward mutations. Our data also indicated that repair of the HBB locus in these embryos occurred preferentially through the non-crossover HDR pathway. Taken together, our work highlights the pressing need to further improve the fidelity and specificity of the CRISPR/Cas9 platform, a prerequisite for any clinical applications of CRSIPR/Cas9-mediated editing.

关键词： CRISPR/Cas9 β-thalassemia humantripronuclear zygotes gene editing homologousrecombination whole-exome sequencing

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永久性氚发光涂料

涂料工业 1990年第5期20卷 34-38页

作者：陈玉玺张家口市发光材料厂

利用放射性β射线激发材料发光的特性,制造永久性发光涂料。这种涂料不需要外激发光源,不消耗电能,结构简单,使用期长,不危害人体。叙述了此涂料的主要技术指标、用途和使用方法,β射线激发发光的原理和特点,放射性同位素和发光基质的选... 详细信息

利用放射性β射线激发材料发光的特性,制造永久性发光涂料。这种涂料不需要外激发光源,不消耗电能,结构简单,使用期长,不危害人体。叙述了此涂料的主要技术指标、用途和使用方法,β射线激发发光的原理和特点,放射性同位素和发光基质的选择,以及氚发光涂料的制造过程等。

关键词：发光涂料氚发光涂料涂料