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MICROPROPAGATION IN TISSUE CULTURE OFDAHURIAN LARCH

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Journal of Northeast Forestry University 1991年第1期2卷 1-8页

作者： lu Zhihua Li Li Liu yuxi Qi LizhiNortheast Forestry University 1. Northeast Forestry University China

Dormant buds of Larix gmelinii (4-30 years old) were cultured in vitro. Axillary buds grew on the explants, and 60%-65% of the explants' axillary buds, a differentiation rate of 60% was obtained on the explants collected from the 30-year-old trees. The maximum number of axillary buds was 26 in one induction per initial explant. Bud clusters were separated into individual buds and most of them elongated into shoots. A few roots grew on the shoots. The MS (Murashige and skoog) and SH (Schenk and Hildebrandt) were more efficient media than the WPM. (Woody Plant Medium). The best hormone Combinations for the axillary bud inductions were BA1+NAA0.01 and BA2+NAA0.2 (mg/L). The procedure was as follows: (1) Apical buds were explanted on the no-hormone basal agar medium and grown for 1 or 2 weeks; (2) Explants were transferred onto the bud-inducing medium and grown for 2 weeks and then (3) Cultured on the basal medium without hormones for axillary bud elongation; (4) Bud clusters were separated and cultured continuously to a minimum height of 1.5cm; (5) shoots were cut at the base and cultured on root-inducing medium; (6) plantlets were allowed to acclimatize and then transferred to the soil.

关键词： Larix gmelinii Micropropagatlon Tissue culture

牛源化脓隐秘杆菌溶血素的重组表达及其间接ELISA检测方法的建立

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黑龙江畜牧兽医 2022年第20期 75-80页

作者：赵宇周思旋卢昱希张涛唐远江陶小艳赵春萍黄波武俊达史开志贵州省农业科学院畜牧兽医研究所贵阳550005

为了建立检测牛源化脓隐秘杆菌抗体的间接ELISA检测方法,试验对分离的牛源化脓隐秘杆菌溶血素(pyolysin,PLO)基因序列进行分析,截去信号肽与跨膜区域后进行碱基优化,合成PLO基因片段,连接至原核表达载体pET-30a中,转化至Arctic Express(... 详细信息

为了建立检测牛源化脓隐秘杆菌抗体的间接ELISA检测方法,试验对分离的牛源化脓隐秘杆菌溶血素(pyolysin,PLO)基因序列进行分析,截去信号肽与跨膜区域后进行碱基优化,合成PLO基因片段,连接至原核表达载体pET-30a中,转化至Arctic Express(DE3)大肠杆菌感受态后诱导表达并纯化,以纯化后的重组蛋白作为抗原包被96孔板,建立牛源化脓隐秘杆菌PLO抗体间接ELISA检测方法,确定检测方法的临界值、特异性、敏感性、重复性,并用建立的方法对临床血清样本进行检测。结果表明:成功构建了重组原核表达质粒pET30a-PLO,实现了重组蛋白在Arctic Express(DE3)大肠杆菌感受态中的表达,并建立了用于检测牛源化脓隐秘杆菌PLO抗体的间接ELISA检测方法。该检测方法的临界值为0.354,与常见牛细菌阳性血清无交叉反应,阳性血清稀释至320倍检测仍高于临界值;批内、批间检测变异系数均小于5%;对10份已知阳性血清进行检测,阳性检出率为100%,80份临床牛血清的阳性检出率为21.25%(17/80)。说明该方法具有良好的特异性、敏感性、重复性,可用于牛源化脓隐秘杆菌感染的快速检测与流行病学调查。

关键词：牛化脓隐秘杆菌溶血素蛋白原核表达间接ELISA 抗体

儿童友好视角下的学前教育设施空间布局及优化研究--以桂林市临桂区为例

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城市建筑 2023年第24期20卷 41-44页

作者：赵珈莹王晓陆玉玺朱文青李福航谢柳娟桂林理工大学土木与建筑工程学院

当前中国进入“老龄化少子化”新常态,学前教育布局的不平衡不充分与人民群众对“幼有所育”的美好期盼存在巨大的矛盾。研究以桂林市临桂区为研究对象,通过运用POI大数据、Python和GIS分析方法,辅以Geo-DA中双变量莫兰指数进行分析。... 详细信息

当前中国进入“老龄化少子化”新常态,学前教育布局的不平衡不充分与人民群众对“幼有所育”的美好期盼存在巨大的矛盾。研究以桂林市临桂区为研究对象,通过运用POI大数据、Python和GIS分析方法,辅以Geo-DA中双变量莫兰指数进行分析。研究发现,学前教育设施存在数量不足、空间分布不均衡、服务覆盖率低等问题。由此建议增加普惠性学前设施供给,增加服务半径,提高服务可达性,优化学前教育空间分布,以推动学前教育的普惠、安全、优质发展,并为教育局等有关部门修订相关政策提供参考依据。

关键词：学前教育设施儿童友好空间布局

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二氧化锰-聚吡咯-改性碳布柔性复合材料的制备及其在超级电容器中的应用

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广东化工 2022年第8期49卷 21-23页

作者：曾永康徐晋扬陈远远卢昱昕黄秋玲常学义东莞理工学院生态环境与建筑工程学院广东东莞523808

本文在活性碳布表面用恒电流法沉积一层聚吡咯,然后再在聚吡咯表面通过水热反应原位生成一层二氧化锰,获得了新型二氧化锰-聚吡咯-改性碳布柔性复合材料。研究表明增加了聚吡咯中间层后的复合物构筑的超级电容器电容性能有显著提升。其... 详细信息

本文在活性碳布表面用恒电流法沉积一层聚吡咯,然后再在聚吡咯表面通过水热反应原位生成一层二氧化锰,获得了新型二氧化锰-聚吡咯-改性碳布柔性复合材料。研究表明增加了聚吡咯中间层后的复合物构筑的超级电容器电容性能有显著提升。其中,C@P@M-400电极获得了1201 m F·cm^(-2)的高比容量;并且其比电容随着电压扫描速率的减小而增大,随着恒流充放电的电流密度的减小而增大。此外,在50 m A·cm^(-2)的电流密度下,C@P@M-400电极经过5000次充放电循环后,容量仍保留了92.0%。这归因于复合材料增加聚吡咯中间层后会显著增加其比表面积,提高离子通过率。

关键词：柔性复合材料二氧化锰聚吡咯改性碳布赝电容水热反应

Unveiling non-radiative center control in CsPbBr_(3) nanocrystals:A comprehensive comparative analysis of hot injection and ligandassisted reprecipitation approaches

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Nano Research 2024年第5期17卷 4525-4534页

作者： Daocheng Hong Yuchen Zhang Shuhan Pan Hanyu Liu Wei Mao Wanli Zhang Yuhui Ye Zhihong Wei Xuxing lu Xiaoyong Wang Zhenda lu yuxi Tian Key Laboratory for Advanced Technology in Environmental Protection of Jiangsu Province Yancheng Institute of TechnologyYancheng 224051China Laboratory of Mesoscopic Chemistry of MOE School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China College of Engineering and Applied Sciences Nanjing UniversityNanjing 210023China National Laboratory of Solid State Microstructures School of PhysicsCollaborative Innovation Center of Advanced MicrostructuresNanjing UniversityNanjing 210093China School of Electronic Electrical Engineering and PhysicsFujian University of TechnologyFuzhou 350118China

Metal-halide perovskite nanocrystals(NCs)have gained significant attention in the field of optoelectronic and photonic devices due to their promising *** their exceptional optical properties,the impact of different synthetic strategies on the fundamental nature of NCs,such as nonradiative recombination centers,remains poorly *** this study,we investigated the photophysical properties of CsPbBr_(3) NCs synthesized using two distinct methods,hot injection and ligand-assisted reprecipitation,at the individual particle *** observed different blinking behaviors under specific photoexcitation power densities and proposed,through intensity-lifetime analysis and Monte-Carlo simulations,that these different synthetic strategies can fabricate NCs with similar crystal structures but distinct surface quenchers with varying energy levels,which significantly affected the photo-induced blinking-down and blinking-up behaviors in individual *** findings indicate a practical and feasible approach for controlling defect engineering in perovskite NCs,with significant implications for their use in optoelectronic and other technological applications.

关键词： metal-halide perovskite nanocrystals photophysical properties nonradiative recombination centers hot injection method ligand-assisted reprecipitation

Effects of local matrix environment on the spectroscopic properties of ensemble to single-particle level carbon dots

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Chinese Chemical Letters 2022年第2期33卷 751-756页

作者： Zhihong Wei Boyang Wang Mingcai Xie Daocheng Hong Xin Yang Sushu Wan Weiqing Yang Siyu lu yuxi Tian Key Laboratory of Mesoscopic Chemistry of MOE School of Chemistry and Chemical EngineeringJiangsu Key Laboratory of Vehicle Emissions ControlNanjing UniversityNanjing 210023China Green Catalysis Center and College of ChemistryZhengzhou UniversityZhengzhou 450001China Key Laboratory for Advanced Technology in Environmental Protection of Jiangsu Province Yancheng Institute of TechnologyYancheng 224051China

Carbon dots(CDs), because of their unique properties, are being rapidly developed as important luminescent materials for imaging, sensing, and use in photonic devices. However, most of the reported fundamental properties of the CDs are results of investigations conducted in the solution state, which may be completely different from those conducted in the solid state. In this work, we study the luminescence properties, photostability, and the dynamics of CDs in different matrix environments, from ensemble to the single-particle level. We observed that the properties associated with the emission centers and photostability of CDs were extremely sensitive to the local chemical environment. A better understanding of the dependence of the spectroscopic properties of CDs on the complex local chemical environment is an important step toward finding new ways of controlling the optical properties of CDs and optimizing their use in various applications.

关键词： Carbon dots Matrix effects Single-particle Photostability Blinking

Optimization of the Plugging Agent Dosage for High Temperature Salt Profile Control in Heavy Oil Reservoirs

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Fluid Dynamics & Materials Processing 2023年第2期19卷 421-436页

作者： Jiayu Ruan Mingjing lu Wei Zhang yuxi Zhang Yuhui Zhou Jie Gong Fan Wang Yuanxiao Guan School of Petroleum Engineering Yangtze UniversityWuhan430000China Petroleum Engineering Technology Research Institute of Shengli Oilfield SINOPECDongying257067China Postdoctoral Scientific Research Working Station of Shengli Oilfield SINOPECDongying257067China Petro China Changqing Oilfield Branch No.1 Gas Production Plant Yan’an716000China

After steam discharge in heavy oil reservoirs,the distribution of temperature,pressure,and permeability in different wells becomes *** channels can easily be produced,which affect the sweep efficiency of the oil *** studies have shown that the salting-out plugging method can effectively block these channels in high-temperature reservoirs,improve the suction profile,and increase oil *** the present study,the optimal dosage of the plugging agent is determined taking into account connection transmissibility and inter-well *** with the connectivity model,a water flooding simulation model is ***,a non-gradient stochastic disturbance algorithm is used to obtain the optimal plugging agent dosage,which provides the basis for the high-temperature salting-out plugging agent adjustment in the field.

关键词： Heavy oil salting out profile control high temperature plugging agent dosage optimization numerical simulation connectivity model

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贵州松桃县猪常见疫病流行病学调查

现代畜牧科技 2023年第9期 35-39页

作者：齐婧张静赵春萍卢昱希谭娅张雄王婧周思旋史开志贵州省农业科学院畜牧兽医研究所贵州贵阳550005 贵州省地方猪保护与繁育工程研究中心贵州贵阳550005

为掌握松桃县规模化猪场常见病毒病和细菌病感染状况和流行情况,本研究采用ELISA试剂盒对384份猪血清样品中常见病毒抗体进行检测,并采用荧光定量PCR方法对80份病死猪肺脏组织中易感病毒和细菌进行核酸检测,对265份鼻拭子进行易感细菌... 详细信息

为掌握松桃县规模化猪场常见病毒病和细菌病感染状况和流行情况,本研究采用ELISA试剂盒对384份猪血清样品中常见病毒抗体进行检测,并采用荧光定量PCR方法对80份病死猪肺脏组织中易感病毒和细菌进行核酸检测,对265份鼻拭子进行易感细菌核酸检测。结果显示,育肥猪、公猪、母猪的口蹄疫、圆环、猪瘟和蓝耳病毒抗体阳性率均在90%以上,公猪和母猪的流行性腹泻阳性抗体率分别为60.00%和59.02%,公猪伪狂犬gE抗体阳性率为6.90%;病死猪圆环病毒2型阳性率为100%、链球菌阳性率为42.5%、蓝耳病毒阳性率为2.5%;断奶仔猪、育肥猪、公猪和母猪的链球菌检出率分别为70.83%、88.67%、100%、96.77%,肺炎支原体检出率分别为37.5%、35.56%、26.67%和11.29%。

关键词：抗体检测病原检测荧光定量PCR 流行病学调查

BMP9 induces osteogenic differentiation through up-regulating LGR4 via the mTORC1/ Stat3 pathway in mesenchymal stem cells

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Genes & Diseases 2024年第3期11卷 468-483页

作者： Jie Zhang Jinhai Jiang Hang Liu Shiyu Wang Kaixin Ke Siyuan Liu Yue Jiang lu Liu Xiang Gao Baicheng He yuxi Su Department of Pharmacology School of PharmacyChongqing Medical UniversityChongqing 400016China Key Laboratory of Biochemistry and Molecular Pharmacology of Chongqing Chongqing Medical UniversityChongqing 400016China Department of Orthopedics Second Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China orthopedics Department Children's Hospital of Chongqing Medical UniversityChongqing 400014China China International Science and Technology Cooperation Base of Child Development and Critical Disorders Jiangxi Hospital Affiliated Children's Hospital of Chongqing Medical UniversityJiangxi 330000China National Clinical Research Center for Child Health and Disorders China

Bone defects and non-union are prevalent in clinical orthopedy,and the outcomes of current treatments are often *** tissue engineering offers a promising approach to treating these conditions *** morphogenetic protein 9(BMP9)can commit mesenchymal stem cells to osteogenic lineage,and a knowledge of the underlying mechanisms may help advance the field of bone tissue ***-rich repeats con-taining G protein-coupled receptor 4(LGR4),a member of G protein-coupled receptors,is essential for modulating bone *** study is aimed at investigating the impact of LGR4 on BMP9-induced osteogenesis in mesenchymal stem cells as well as the underlying *** marrow stromal cells from BMp9-knockout mice exhibited diminished LGR4 expression,and exogenous LGR4 clearly restored the impaired osteogenic potency of the bone marrow stromal ***,LGR4 expression was increased by BMP9 in C3H10T1/2 ***4 augmented the benefits of BMP9-induced osteogenic markers and bone formation,whereas LGR4 inhibition restricted these ***,the BMP9-induced li-pogenic markers were increased by LGR4 *** protein levels of Raptor and p-Stat3 were elevated by *** knockdown or p-Stat3 suppression attenuated the osteoblastic markers and LGR4 expression brought on by ***4 significantly reversed the blocking ef-fect of Raptor knockdown or p-Stat3 suppression on the BMP9-induced osteoblastic *** interacts with p-Stat3,and p-Stat3 activates the LGR4 promoter *** conclusion,LGR4 boosts BMP9 osteoblastic potency in mesenchymal stem cells,and BMP9 may up-regulate LGR4 via the mTORC1/Stat3 signal activation.

关键词： BMP9 LGR4 mTORC1 Osteogenic differentiation Stat3