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Analyzing the cellular and molecular atlas of ovarian mesenchymal cells provides a strategy against female reproductive aging

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Science China(Life Sciences) 2023年第12期66卷 2818-2836页

作者： Longzhong Jia Wenji Wang Jing Liang Shudong Niu Yibo Wang Jian Yang Lingyu Li Ge Wang Xueqiang Xu Lu Mu Kaixin Cheng Xuebing Yang Yijing Wang Haoshu Luo Guoliang Xia yuwen ke Yan Zhang Hua Zhang State key Laboratory of Animal Biotech Breeding College of Biological SciencesChina Agricultural UniversityBeijing 100193China School of Life Sciences Taizhou UniversityTaizhou 318000China National Institute of Biological Sciences Beijing 102206China key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in Western China College of Life ScienceNingxia UniversityYinchuan 750021China

Ovarian mesenchymal cells(oMCs)constitute a distinct microenvironment that supports folliculogenesis under physiological conditions.Supplementation of exogenous non-ovarian mesenchymal-related cells has been reported to be an efficient approach to improve ovarian functions.However,the development and cellular and molecular characteristics of endogenous oMCs remain largely unexplored.In this study,we surveyed the single-cell transcriptomic landscape to dissect the cellular and molecular changes associated with the aging of oMCs in mice.Our results showed that the oMCs were composed of five ovarian differentiatedMC(odMC)populations and one ovarian mesenchymal progenitor(oMP)cell population.These cells could differentiate into various odMCs via an oMP-derived route to construct the ovarian stroma structures.Comparative analysis revealed that ovarian aging was associated with decreased quantity of oMP cells and reduced quality of odMCs.Based on the findings of bioinformatics analysis,we designed different strategies involving supplementation with young oMCs to examine their effects on female fertility and health.Our functional investigations revealed that oMCs supplementation prior to ovarian senescence was the optimal method to improve female fertility and extend the reproductive lifespan of aged females in the longterm.

关键词： single-cell analysis ovary mesenchyma mesenchymal progenitor cells aging infertility

Aurora B kinase activation requires survivin priming phosphorylation by PLK1

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分子细胞生物学报:英文版 2011年第4期3卷 260-267页

作者： Youjun Chu Phil Y.Yao Wenwen Wang Dongmei Wang Zhikai Wang Liangyu Zhang Yuejia Huang yuwen ke Xia Ding Xuebiao Yao Anhui key Laboratory of Cellular Dynamics and Chemical Biology University of Science and Technology of ChinaHefei 230027China Department of Physiology Morehouse School of MedicineAtlantaGA 30310USA Department of Medicine Beijing University of Chinese MedicineBeijing 100086China

During cell division,chromosome segregation is orchestrated by the interaction of spindle microtubules with the centromere.Accurate attachment of spindle microtubules to kinetochore requires the chromosomal passenger of Aurora B kinase complex with borealin,INCENP and survivin(SUR).The current working model argues that SUR is responsible for docking Aurora B to the centromere whereas its precise role in Aurora B activation has been unclear.Here,we show that Aurora B kinase activation requires SUR priming phosphorylation at Ser20 which is catalyzed by polo-like kinase 1(PLK1).Inhibition of PLK1 kinase activity or expression of non-phosphorylatable SUR mutant prevents Aurora B activation and correct spindle microtubule attachment.The PLK1-mediated regulation of Aurora B kinase activity was examined in real-time mitosis using fluorescence resonance energy transfer-based reporter and quantitative analysis of native Aurora B substrate phosphorylation.We reason that the PLK1-mediated priming phosphorylation is critical for orchestrating Aurora B activity in centromere which is essential for accurate chromosome segregation and faithful completion of cytokinesis.

关键词： Aurora B kinase sensor phospho-CENP-A PLK1 survivin

Load and velocity boundaries of oil-based superlubricity using 1,3-diketone

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Friction 2023年第5期11卷 704-715页

作者： Yuyang YUAN Tobias AMANN yuwen XU Yan ZHANG Jingfu CHEN Chenqing YUAN ke LI School of Transportation and Logistics Engineering Wuhan University of TechnologyWuhan 430063China Reliability Engineering Institute National Engineering Research Center for Water Transport SafetyMOSTWuhan 430063China Fraunhofer Institute for Mechanics of Materials IWM Freiburg 79108Germany

The clarification of the critical operating conditions and the failure mechanism of superlubricity systems is of great significance for seeking appropriate applications in industry.In this work,the superlubricity region of 1,3-diketone oil EPND(1-(4-ethyl phenyl)nonane-1,3-dione)on steel surfaces was identified by performing a series of ball-on-disk rotation friction tests under various normal loads(3.5–64 N)and sliding velocities(100–600 mm/s).The result shows that beyond certain loads or velocities superlubricity failed to be reached due to the following negative effects:(1)Under low load(≤3.5 N),insufficient running-in could not ensure good asperity level conformity between the upper and lower surfaces;(2)the high load(≥64 N)produced excessive wear and big debris;(3)at low velocity(≤100 mm/s),the weak hydrodynamic effect and the generated debris deteriorated the lubrication performance;(4)at high velocity(≥500 mm/s),oil migration occurred and resulted in oil starvation.In order to expand the load and velocity boundaries of the superlubricity region,an optimized running-in method was proposed to avoid the above negative effects.By initially operating a running-in process under a suitable combination of load and velocity(e.g.16 N and 300 mm/s)and then switching to the target certain higher or lower load/velocity(e.g.100 N),the superlubricity region could break through its original boundaries.The result of this work suggests that oil-based superlubricity of 1,3-diketone is a promising solution to friction reduction under suitable operating conditions especially using a well-designed running-in strategy.

关键词： macroscopic superlubricity 1,3-diketone oil running-in process load and velocity boundaries

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基于图像技术的爆区粉尘浓度测量方法研究

矿业研究与开发 2024年第4期44卷 168-174页

作者：余晓轲柯丽华张光权胡南燕蒋玉文黄兆云武汉科技大学资源与环境工程学院湖北武汉430081 湖北景深安全技术有限公司湖北宜昌市443000

针对露天矿爆区粉尘浓度不易测量的问题,提出了一种基于图像技术的爆区粉尘浓度测量方法。采集爆破区域内粉尘图像及典型测点的粉尘浓度信息,优选高斯滤波降噪方法并确定灰度级α为128,对粉尘图像进行降噪、增强和分割处理,有效识别粉... 详细信息

针对露天矿爆区粉尘浓度不易测量的问题,提出了一种基于图像技术的爆区粉尘浓度测量方法。采集爆破区域内粉尘图像及典型测点的粉尘浓度信息,优选高斯滤波降噪方法并确定灰度级α为128,对粉尘图像进行降噪、增强和分割处理,有效识别粉尘区域与背景区域边界,提高了粉尘图像灰度信息提取的准确性。采用函数拟合法,优化建立爆区粉尘浓度和灰度值的函数为:C=-0.01157G^(2)+2.342G-104.1,并应用于矿山爆区粉尘浓度测量中。结果表明:爆区粉尘浓度在起爆后第7秒达到峰值13.824 mg/m^(3),在第90秒下降到3.749 mg/m^(3);在第6秒时,爆区左后方的高浓度粉尘云团处于距地表3~52 m高度,其影响区域范围为110 m×60 m,爆区右上方的高浓度粉尘云团处于距地表52 m以上的位置,其影响区域范围为59 m×56 m;在第90秒时,粉尘云团集中在距地表40 m以上的位置,影响范围为120 m×67 m,与实际情况相符,验证了所提出的粉尘浓度测量方法的有效性。

关键词：露天矿图像技术爆破粉尘粉尘浓度灰度值

Genome wide abnormal DNA methylome of human blastocyst in assisted reproductive technology

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Journal of Genetics and Genomics 2017年第10期44卷 475-481页

作者： Guoqiang Li Yang Yu Yong Fan Congru Li Xiaocui Xu Jialei Duan Rong Li Xiangjin Kang Xin Ma Xuepeng Chen yuwen ke Jie Yan Ying Lian Ping Liu Yue Zhao Hongcui Zhao Yaoyong Chen Xiaofang Sun Jianqiao Liu Jie Qiao Jiang Liu Ministry of Education key Laboratory of Assisted Reproduction Beijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductiveTechnology Center of Reproductive Medicine Peking University Third Hospital Beijing 100191 China GAS key Laboratory of Genome Sciences and Information Collaborative Innovation Center of Genetics and Development Beijing Institute of GenomicsGAS Beijing 160101 China key Laboratory for Major Obstetric Diseases of Guangdong Province The Third Affiliated Hospital of Guangzhou Medical University Guangzhou 510150China University of Chinese Academy of Sciences Beijing 100029 China

Proper reprogramming of parental DNA methylomes is essential for mammalian embryonic development. However, it is unknown whether abnormal methylome reprogramming occurs and is associated with the failure of embryonic development. Here we analyzed the DNA methylomes of 57 blastocysts and 29 trophectoderm samples with different morphological grades during assisted reproductive technology （ART） practices. Our data reveal that the global methylation levels of high-quality blastocysts are similar （0.30 ± 0.02, mean ± SD）, while the methylation levels of low-quality blastocysts are divergent and away from those of high-quality blastocysts. The proportion of blastocysts with a methylation level falling within the range of 0.30± 0.02 in different grades correlates with the live birth rate for that grade. Moreover, abnormal methylated regions are associated with the failure of embryonic development. Furthermore, we can use the methylation data of cells biopsied from trophectoderm to predict the blastocyst methylation level as well as to detect the aneuploidy of the blastocysts. Our data indicate that global abnormal methylome reprogramming often occurs in human embryos, and suggest that DNA methylome is a potential biomarker in blastocyst selection in ART.

关键词： Human Abnormal Blastocyst Methylome ART

基于古代文献计量分析的经典名方苏葶丸关键信息考证

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中国医院用药评价与分析 2024年第2期24卷 157-160,164页

作者：王薇王岚柯苹叶玉雯章春晓陈岚贡磊磊空军杭州特勤疗养中心疗养三区药械科杭州310002 中国中医科学院中药研究所北京100700 首都医科大学附属北京妇产医院药学部北京100026

目的:系统性梳理经典名方苏葶丸的相关古籍以及现代研究文献,进行关键信息的考证,并总结古今应用情况,为后续复方制剂的深入研究和开发提供科学依据。方法:采用文献计量学方法,以“苏葶丸”为关键词检索相关古籍文献及现代临床研究文献... 详细信息

目的:系统性梳理经典名方苏葶丸的相关古籍以及现代研究文献,进行关键信息的考证,并总结古今应用情况,为后续复方制剂的深入研究和开发提供科学依据。方法:采用文献计量学方法,以“苏葶丸”为关键词检索相关古籍文献及现代临床研究文献。依据纳入、排除标准进行筛选,选取苏葶丸的处方来源、功能主治、组成、药物炮制、用法与用量和临床研究等信息,以考证苏葶丸的古代文献及现代临床应用。结果:经古籍文献检索,苏葶丸首载于清代吴谦所著的《医宗金鉴》,由紫苏子和葶苈子组成,功效为泻肺平喘,随着后世流传,组成及功效主治基本延续至今,其服用剂量及服用方法略有变化。检索其现代应用发现,苏葶丸常与其他方剂合用来治疗心脑血管系统、呼吸系统疾病。结论:通过对苏葶丸古今文献进行分析考证,明确了苏葶丸的处方来源、药物基原、炮制规格、功效和用法与用量等关键问题,也对其现代临床应用进行了总结,为苏葶丸的制剂开发及后续深入研究提供了参考依据。

关键词：经典名方苏葶丸历史沿革文献分析

Genome-wide analyses on transcription factors and their potential microRNA regulators involved in maize male fertility

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The Crop Journal 2021年第6期9卷 1248-1262页

作者： Ziwen Li Taotao Zhu Shuangshuang Liu Yilin Jiang Haoyun Liu yuwen Zhang ke Xie Jinping Li Xueli An Xiangyuan Wan Zhongzhi International Institute of Agricultural Biosciences Biology and Agriculture Research CenterUniversity of Science and Technology BeijingBeijing 100024China Beijing Engineering Laboratory of Main Crop Bio-Tech Breeding Beijing International Science and Technology Cooperation Base of Bio-Tech BreedingBeijing 100192China

Anther development is a programmed biological process crucial to plant male reproduction. Genomewide analyses on the functions of transcriptional factor(TF) genes and their microRNA(miRNA) regulators contributing to anther development have not been comprehensively performed in maize. Here, using published RNA-Seq and small RNA-Seq(sRNA-Seq) data from maize anthers at ten developmental stages in three genic male-sterility(GMS) mutants(ocl4, mac1, and ms23) and wild type W23, as well as newly sequenced maize anther transcriptomes of ms7-6007 and lob30 GMS mutants and their WT lines, we analyzed and found 1079 stage-differentially expressed(stage-DE) TF genes that can be grouped into six(premeiotic, meiotic, postmeiotic, premeiotic-meiotic, premeiotic-postmeiotic, and meiotic-postmeiotic clusters) expression clusters. Functional enrichment combined with cytological and physiological analyses revealed specific functions of genes in each expression cluster. In addition, 118 stage-DE miRNAs and99 miRNA-TF gene pairs were identified in maize anthers. Further analyses revealed the regulatory roles of zma-miR319 and zma-miR159 as well as ZmMs7 and ZmLOB30 on ZmGAMYB expression. Moreover,ZmGAMYB and its paralog ZmGAMYB-2 were demonstrated as novel maize GMS genes by CRISPR/Cas9 knockout analysis. These results extend our understanding on the functions of miRNA-TF gene regulatory pairs and GMS TF genes contributing to male fertility in plants.

关键词： Transcription factor gene microRNA Anther development Male fertility Genic male sterility

Influence of MoSi_(2) on oxidation protective ability of TaB_(2)-SiC coating in oxygen-containing environments within a broad temperature range

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Journal of Advanced Ceramics 2020年第6期9卷 703-715页

作者： Xuanru REN Junshuai LV Wei LI yuwen HU ke SUN Can MA Hong’ao CHU Weiguang WANG Leihua XU Ziyu LI Peizhong FENG School of Materials Science and Engineering China University of Mining and TechnologyXuzhou 221116China

TaB_(2)-SiC coating modified by different content of MoSi_(2) was fabricated on graphite substrate with SiC inner coating by liquid phase sintering to elevate the anti-oxidation capability of the TaB_(2)-SiC coatings.As compared to the sample with the TaB_(2)-40wt% SiC coating,the coating sample modified with MoSi_(2) exhibited a weight gain trend at lower temperatures,the fastest weight loss rate went down by 76%,and the relative oxygen permeability value reduced from about 1% to near 0.More importantly,the large amount of SiO_(2) glass phase produced over the coating during oxidation was in contact with the modification of MoSi_(2),which was proved to be beneficial to the dispersion of Ta-oxides.A concomitantly formed continuous Ta-Si-O-B compound glass layer showed excellent capacity to prevent oxygen penetration.However,when the TaB_(2) content was sacrificed to increase the MoSi_(2) content,the relative oxygen permeability of the coating increased instead of decreased.Thus,on the basis of ample TaB_(2) content,increasing the MoSi_(2) content of the coating is conducive to reducing the relative oxygen permeability of the coatings in a broad temperature region.

关键词： MoSi_(2) TaB_(2)-SiC coating liquid phase sintering compound glass layer relative oxygen permeability

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巴基斯坦N区块浪控三角洲沉积相划分及模式

沉积学报 2022年第1期40卷 255-266页

作者：陈旭欧荣生斯尚华董玉文柯钦长江大学地球科学学院武汉430100 构造与油气资源教育部重点实验室(中国地质大学) 武汉430074 青海油田公司勘探事业部甘肃敦煌736202 西安石油大学地球科学与工程学院西安710065 中石油东方地球物理公司研究院河北涿州072750

浪控三角洲砂体是海陆交互带沉积有利的油气储层和勘探目标,目前业界对其微相划分、沉积机理和沉积模式研究较少。以巴基斯坦N区块为例,依据岩心、测井、地震及分析测试资料,开展受波浪改造作用的海相三角洲沉积相带划分、展布及沉积模... 详细信息

浪控三角洲砂体是海陆交互带沉积有利的油气储层和勘探目标,目前业界对其微相划分、沉积机理和沉积模式研究较少。以巴基斯坦N区块为例,依据岩心、测井、地震及分析测试资料,开展受波浪改造作用的海相三角洲沉积相带划分、展布及沉积模式研究。结果表明:综合大量沉积相标志分析,研究区白垩系处于砂质高能海岸带,三角洲入海口受到强烈的波浪、沿岸流和风暴浪的改造、破坏和再分配作用,形成浪控三角洲沉积,表现为沿平原亚相向海洋延伸的环岸砂坝发育带。其格架相与建设性三角洲明显不同,水下分流河道和河口砂坝不发育,在河口两侧形成滩砂复合沉积,具有砂岩质纯、厚层、平行海岸线、大面积连片分布、延伸规模广的特点,构成了浪控三角洲骨干砂体类型。外前缘相带始终受波浪冲洗和扰动,形成了平坦、席状的砂泥互层。平面上为向广海方向凸出的朵叶状轮廓,垂向上为下细上粗的反旋回,微相类型保存不完整,为破坏性三角洲沉积。最后总结了实例区浪控三角洲沉积模式,为三角洲储层评价提供基础。

关键词：沉积相标志浪控三角洲沉积微相沉积模式