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米糠纳米纤维素的制备表征及其细胞毒性

中国粮油学报 2024年第4期39卷 41-48页

作者：郭锐林郭雨桐孟可瑜梁富强石嘉怿南京财经大学食品科学与工程学院南京210023

以提取的米糠纤维素(纤维素质量分数90.22%)为原料,采用不同体积分数硫酸水解(60%、64%和68%)制备获得米糠纳米纤维素(Rice bran nanocellulose,RBNC),对其表面形貌、尺寸、电位、化学结构、热稳定性及晶体结构进行了表征,并进一步评价... 详细信息

以提取的米糠纤维素(纤维素质量分数90.22%)为原料,采用不同体积分数硫酸水解(60%、64%和68%)制备获得米糠纳米纤维素(Rice bran nanocellulose,RBNC),对其表面形貌、尺寸、电位、化学结构、热稳定性及晶体结构进行了表征,并进一步评价了其细胞毒性。透射电镜和原子力显微镜结果显示,制备得到的RBNC是长度在196.45~257.26 nm、直径为8.46~16.34 nm、长径比为17.06~24.34范围内的针状粒子;Zeta电位值在-32.9~-35.2 mV范围内;红外光谱和X-射线衍射分析表明得到的RBNC化学结构未发生变化,仍保留纤维素Ⅰ型晶体结构。体积分数为64%的硫酸水解得到的RBNC长径比最优(24.34±3.63)、结晶度最高(81.32%)、Zeta电位绝对值最大(-35.20±0.85)mV。进一步的细胞毒性评价表明,RBNC分别在≤2000μg/mL和≤500μg/mL质量浓度范围内,对Caco-2和HepG-2细胞无显著毒性,表现出良好的生物相容性。

关键词：米糠纤维素纳米纤维素细胞毒性

九翅豆蔻叶绿体基因组特征及其密码子偏好性分析

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基因组学与应用生物学 2024年第1期43卷 17-30页

作者：傅武祥郭雨桐张雪梅马占霞滇西应用技术大学普洱茶学院普洱665000 云南农业大学农学与生物技术学院昆明650201 中国科学院西双版纳热带植物园勐仑666303

为探究九翅豆蔻(Amomum maximum)叶绿体全基因组(chloroplast DNA,cpDNA)特征和密码子使用偏好性,本研究利用Illumina HiSeq测序平台对九翅豆蔻叶绿体全基因组进行测序,通过组装与注释得到叶绿体基因组全序列,使用MISA、IQTREE和CodonW... 详细信息

为探究九翅豆蔻(Amomum maximum)叶绿体全基因组(chloroplast DNA,cpDNA)特征和密码子使用偏好性,本研究利用Illumina HiSeq测序平台对九翅豆蔻叶绿体全基因组进行测序,通过组装与注释得到叶绿体基因组全序列,使用MISA、IQTREE和CodonW等软件对九翅豆蔻叶绿体全基因组的微卫星位点(microsatellite site)、密码子偏好性及系统发育进行分析。结果表明,九翅豆蔻完整叶绿体全基因组长度为163602 bp,基因组平均G+C含量为36.1%,其大单拷贝(large single copy,LSC)区和小单拷贝(small single copy,SSC)区长度分别为88626 bp和15426 bp,两个反向重复(inverted repeat,IR)区长度为29775 bp。九翅豆蔻叶绿体基因组共编码132个基因,包含86个蛋白编码基因、38个tRNA基因和8个rRNA基因。叶绿体基因组序列上共检测到132个简单重复序列(simple sequence repeat,SSR)位点。其中单核苷酸、二核苷酸、三核苷酸、四核苷酸和五核苷酸数量分别为65、35、5、24、3个,绝大多数属于单碱基重复的A/U类型。系统发育分析显示九翅豆蔻在豆蔻属(Amomum)植物中单独聚为一支。这为九翅豆蔻种质资源鉴定、评价及物种形成机制研究奠定了基础。

关键词：九翅豆蔻叶绿体基因组微卫星位点密码子偏好性系统发育分析

COL4A5基因突变致X连锁Alport综合征的1例家系

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中国优生与遗传杂志 2024年第2期32卷 355-359页

作者：郭禹佟李深王志伟北京中医药大学北京100029 中国中医科学院广安门医院北京100053 烟台市牟平区中医医院山东烟台264100

Alport综合征是最常见的遗传性肾脏病之一。其临床表型除与该病相关的基因变异相关,还同其他基因状况、生活环境等因素密切相关,故即使同一家系中拥有相近基因型的个体也会出现不同的疾病表现。本研究对中国中医科学院广安门医院1例具... 详细信息

Alport综合征是最常见的遗传性肾脏病之一。其临床表型除与该病相关的基因变异相关,还同其他基因状况、生活环境等因素密切相关,故即使同一家系中拥有相近基因型的个体也会出现不同的疾病表现。本研究对中国中医科学院广安门医院1例具有复杂家系的慢性肾脏病患者开展调查,收集患者及其家族成员临床资料,采用三代基因测序技术对患者及多位亲属的外周血DNA进行遗传学分析,结果显示家族中多人为COL4A5[c.152G>A(***51Glu)]基因突变导致的Alport综合征。文章就X连锁Alport综合征的临床特点及预后进行文献复习,结合对该家系多个受累成员发病情况分析,旨在提高临床医生对该病特点及遗传规律的认识。

关键词：肾小球病 X连锁Alport综合征 COL4A5基因突变

基于改进NSGA-Ⅱ的交叉口信号配时多目标优化

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青岛理工大学学报 2024年第1期45卷 111-117,125页

作者：陈秀锋王瑞聪陈咨羽高梦圆郭玉彤青岛理工大学土木工程学院青岛266525

针对城市交叉口信号配时多目标优化的求解效率问题,设计了一种基于改进NSGA-Ⅱ的城市交叉口信号配时优化方案。该方法采纳车辆延误、停车次数、通行能力3个评价指标设计了多目标信号配时优化模型,并提出了改进的NSGA-Ⅱ(NSGA-Ⅱ-DE)对... 详细信息

针对城市交叉口信号配时多目标优化的求解效率问题,设计了一种基于改进NSGA-Ⅱ的城市交叉口信号配时优化方案。该方法采纳车辆延误、停车次数、通行能力3个评价指标设计了多目标信号配时优化模型,并提出了改进的NSGA-Ⅱ(NSGA-Ⅱ-DE)对配时模型进行求解。NSGA-Ⅱ-DE采用DE/Rand/1的全局变异策略对NSGA-Ⅱ算法选择策略进行改进,并设计了种群动态更新算法,提高了算法的收敛速度和求解精度。结果表明改进算法获得了较好的Pareto最优解,通过典型交叉口仿真验证,相对于NSGA-Ⅱ算法,NSGA-Ⅱ-DE方法设计配时方案平均车辆延误优化提升13.42%,排队长度优化提升16.73%,平均停车次数优化提升15.50%,能够实现城市交叉口的最优化控制。

关键词：交通工程信号配时多目标优化 T形交叉口城市交通相位 NSGA-Ⅱ-DE算法全局变异

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Calhex231通过细胞焦亡改善大鼠心肌梗死面积及心肌纤维化

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心血管病学进展 2024年第4期45卷 379-384页

作者：刘文秀郭雨桐孙雪宋琳琳刘越丁雪哈尔滨医科大学附属第一医院心内科黑龙江哈尔滨150001

目的研究钙敏感受体(CaSR)负性变构调节剂Calhex231是否能改善大鼠的心肌梗死(MI)面积和心肌纤维化。方法健康Wistar大鼠随机分为3组:sham组、MI组和MI+Calhex231组。TTC染色评估MI面积和坏死情况。Masson染色、免疫组织化学及电镜检查... 详细信息

目的研究钙敏感受体(CaSR)负性变构调节剂Calhex231是否能改善大鼠的心肌梗死(MI)面积和心肌纤维化。方法健康Wistar大鼠随机分为3组:sham组、MI组和MI+Calhex231组。TTC染色评估MI面积和坏死情况。Masson染色、免疫组织化学及电镜检查心肌纤维化、Ⅲ型胶原蛋白及成纤维细胞胶原合成情况。免疫荧光和Western blot检测CaSR表达变化。Western blot检测含NOD样受体热蛋白结构域相关蛋白3(NLRP3)、胱天蛋白酶-1(Casp-1)、gasdermin D(GSDMD)、GSDMD N-末端结构域(NT-GSDMD)和白细胞介素-1β(IL-1β)的蛋白表达变化。并采用免疫组织化学法评估NT-GSDMD和IL-1β的表达情况。结果与sham组相比,MI组大鼠MI面积和纤维化明显增加,心肌组织Ⅲ型胶原蛋白沉积和成纤维细胞胶原合成明显增加,而且CaSR、NLRP3、Casp-1、GSDMD、NT-GSDMD和IL-1β表达水平也明显升高。进一步免疫组织化学染色确认了NT-GSDMD和IL-1β表达增加。MI+Calhex231组与MI组相比较,Calhex231可抑制上述改变。结论Calhex231可通过CaSR抑制细胞焦亡和Ⅲ型胶原蛋白沉积,改善大鼠MI面积和心肌纤维化,有助于Calhex231在心肌纤维化疾病中临床转化。

关键词：钙敏感受体细胞焦亡心肌梗死纤维化

iHypoxia:An Integrative Database of Protein Expression Dynamics in Response to Hypoxia in Animals

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Genomics, Proteomics & Bioinformatics 2023年第2期21卷 267-277页

作者： Ze-Xian Liu Panqin Wang Qingfeng Zhang Shihua Li Yuxin Zhang yutong guo Chongchong Jia Tian Shao Lin Li Han Cheng Zhenlong Wang State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer MedicineSun Yat-sen University Cancer CenterSun Yat-sen UniversityGuangzhou 510060China School of Life Sciences Zhengzhou UniversityZhengzhou 450001China

Mammals have evolved mechanisms to sense hypoxia and induce hypoxic ***,high-throughput techniques have greatly promoted global studies of protein expression changes during hypoxia and the identification of candidate genes associated with hypoxiaadaptive evolution,which have contributed to the understanding of the complex regulatory networks of *** this study,we developed an integrated resource for the expression dynamics of proteins in response to hypoxia(iHypoxia),and this database contains 2589 expression events of 1944 proteins identified by low-throughput experiments(LTEs)and 422,553 quantitative expression events of 33,559 proteins identified by high-throughput experiments from five mammals that exhibit a response to *** experimental details,such as the hypoxic experimental conditions,expression patterns,and sample types,were carefully collected and ***,8788 candidate genes from diverse species inhabiting low-oxygen environments were also *** addition,we conducted an orthologous search and computationally identified 394,141 proteins that may respond to hypoxia among 48 *** enrichment analysis of human proteins identified from LTEs shows that these proteins are enriched in certain drug targets and cancer *** of known posttranslational modification(PTM)sites in the proteins identified by LTEs reveals that these proteins undergo extensive PTMs,particularly phosphorylation,ubiquitination,and *** provides a convenient and user-friendly method for users to obtain hypoxia-related information of interest.

关键词： Hypoxia Expression dynamics Low-throughput experiment High-throughput experiment Functional annotation

Bacteria-responsive programmed self-activating antibacterial hydrogel to remodel regeneration microenvironment for infected wound healing

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National Science Review 2024年第4期11卷 254-270页

作者： yutong Yang Jiaxin Wang Shengfei Huang Meng Li Jueying Chen Dandan Pei Zhen Tang Baolin guo State Key Laboratory for Mechanical Behavior of Materials and Frontier Institute of Science and Technology and Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine ResearchCollege of Stomatology Xi'an Jiaotong University Department of Orthopedics Tangdu Hospital Fourth Military Medical University Department of Orthopedics The First Affiliated Hospital of Xi'an Jiaotong University

There is still an urgent need to develop hydrogels with intelligent antibacterial ability to achieve on-demand treatment of infected wounds and accelerate wound healing by improving the regeneration microenvironment. We proposed a strategy of hydrogel wound dressing with bacteria-responsive self-activating antibacterial property and multiple nanozyme activities to remodel the regeneration microenvironment in order to significantly promote infected wound healing. Specifically, p H-responsive H2O2self-supplying composite nanozyme（MSCO） and p H/enzyme-sensitive bacteria-responsive triblock micelles encapsulated with lactate oxidase（PPEL） were prepared and encapsulated in hydrogels composed of L-arginine-modified chitosan（CA） and phenylboronic acid-modified oxidized dextran（ODP） to form a cascade bacteria-responsive self-activating antibacterial composite hydrogel platform. The hydrogels respond to multifactorial changes of the bacterial metabolic microenvironment to achieve on-demand antibacterial and biofilm eradication through transformation of bacterial metabolites, and chemodynamic therapy enhanced by nanozyme activity in conjunction with self-driven nitric oxide（NO） release. The composite hydrogel showed 'self-diagnostic' treatment for changes in the wound *** self-activating antibacterial therapy in the infection stage to self-adaptive oxidative stress relief and angiogenesis in the post-infection stage, it promotes wound closure, accelerates wound collagen deposition and angiogenesis, and completely improves the microenvironment of infected wound regeneration, which provides a new method for the design of intelligent wound dressings.

关键词： bacterial response hydrogel dressing multiple nanozyme activity biofilm elimination infected motion wound healing

Cardiac energy metabolism disorder mediated by energy substrate imbalance and mitochondrial damage upon tebuconazole exposure

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Journal of Environmental Sciences 2024年第2期136卷 270-278页

作者： Tingting Ku Jindong Hu Mengmeng Zhou Yuanyuan Xie yutong Liu Xin Tan Lin guo Guangke Li Nan Sang College of Environmental Science and Resources Research Center of Environment and HealthShanxi UniversityTaiyuan 030006China

Tebuconazole exposure has been described as an increasing hazard to human *** increasing number of recent studies have shown a positive association between tebuconazole exposure and cardiovascular disease risk,which is characterized by the reduction of adenosine triphosphate(ATP)***,researches on the damage of tebuconazole exposure to energy metabolism and the related molecular mechanisms are *** the present study,male C57BL/6 mice were treated with tebuconazole at different low concentrations for 4 *** results indicated that tebuconazole could accumulate in the heart and further induce the decrease of ATP content in the mouse ***,tebuconazole induced an obvious shift in substrate utilization of fatty acid and glucose by disrupting their corresponding transporters(GLUT1,GLUT4,CD36,FABP3 and FATP1)expression,and significantly repressed the expression of mitochondrial biogenesis(Gabpa and Tfam)and oxidative phosphorylation(CS,Ndufa4,Sdhb,Cox5a and Atp5b)related genes in a dosedependent *** investigation revealed that these alterations were related to the IRS1/AKT and PPARγ/RXRα*** findings contribute to a better understanding of triazole fungicide-induced cardiovascular disease by revealing the key indicators associated with this phenomenon.

关键词： Tebuconazole Cardiotoxicity Energy metabolic disorders Energy substrate Mitochondrial damage

Sparse identification-assisted exploration of the atomic-scale deformation mechanism in multiphase CoCrFeNi high-entropy alloys

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Science China(Technological Sciences) 2024年第4期67卷 1124-1132页

作者： XIAO Lu guo XiaoXiang SUN yutong WANG Gang LONG WeiMin LIAW Peter K. REN JingLi School of Mathematics and Statistics Zhengzhou UniversityZhengzhou 450001China Academy of Mathematics and Systems Science Chinese Academy of SciencesBeijing 100190China Institute of Materials Shanghai UniversityShanghai 200444China SKL of Advanced Brazing Metals&Technology Zhengzhou Research Institute of Mechanical EngineeringZhengzhou 450001China Department of Materials Science and Engineering The University of TennesseeKnoxvilleTN 37996USA

This study investigated the atomic-scale deformation mechanism of multiphase CoCrFeNi high-entropy alloys(HEAs)at liquid helium,liquid nitrogen,and room temperatures.A million-atom multiphase HEA was prepared using molecular dynamics simulation involving melt and quench *** HEA exhibited high-density dislocations and some twins,consistent with experimental *** analysis revealed an inconsistent evolution of the microstructure under tensile *** particular,the elastic and initial plastic stages exhibited an increase in the disordered structure at the expense of the face-centered cubic and hexagonal close-packed structures,followed by a subsequent transformation involving multiple structural ***,through sparse identification,a model depicting microstructural evolution during tension was extracted for the CoCrFeNi HEA at three typical temperatures and three tensile *** model highlighted the importance of the body-centered cubic structure in the evolutionary process.

关键词： high-entropy alloy data-driven method microstructure evolution multiphase structure molecular dynamics simulation