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Differential response of radial growth and δ^(13)C in Qinghai spruce(Picea crassifolia) to climate change on the southern and northern slopes of the Qilian Mountains in Northwest China

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Journal of Forestry Research 2024年第3期35卷 205-218页

作者： Li Qin Huaming shang Weiping Liu yuting Fan Kexiang Liu Tongwen Zhang Ruibo Zhang Key Laboratory of Tree-Ring Physical and Chemical Research(CMA)/Xinjiang Key Laboratory of Tree-Ring Ecology Institute of Desert MeteorologyChina Meteorological AdministrationUrimqi 830002People's Republicof China Xinjiang Meteorological Information Center Urimqi 830002People's Republic of China

Tree radial growth can have significantly differ-ent responses to climate change depending on the *** elucidate the effects of climate on radial growth and stable carbon isotope(δ^(13)C)fractionation of Qing-hai spruce(Picea crassifolia),a widely distributed native conifer in northwestern China in different environments,we developed chronologies for tree-ring widths and δ^(13)C in trees on the southern and northern slopes of the Qilian Mountains,and analysed the relationship between these tree-ring variables and major climatic ***-ring widths were strongly influenced by climatic factors early in the growing season,and the radial growth in trees on the northern slopes was more sensitive to climate than in trees on the ***-ring δ^(13)C was more sensitive to climate than radial growth.δ^(13)C fractionation was mainly influenced by summer temperature and precipitation early in the growing *** conductance more strongly limited stable carbon isotope fractionation in tree rings than photosynthetic rate *** response between tree rings and climate in mountains gradually weakened as climate *** in radial growth and stable carbon isotope fractionation of *** in response to climate in the Qilian Mountains may be further complicated by continued climate change.

关键词： Tree rings Qinghai spruce(Picea crassifolia Kom.) Stable carbon isotope(δ^(13)C) Qilian Mountains:Climate change

Quantum Dot Nanobeads-Labelled Lateral Flow Immunoassay Strip for Rapid and Sensitive Detection of Salmonella Typhimurium Based on Strand Displacement Loop-Mediated Isothermal Amplification

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Engineering 2022年第12期19卷 62-70页

作者： yuting shang Shuzhen Cai Qinghua Ye Qingping Wu Yanna Shao Xiaoying Qu Xinran Xiang Baoqing Zhou Yu Ding Moutong Chen Liang Xue Honghui Zhu Jumei Zhang Guangdong Provincial Key Laboratory of Microbial Safety and Health&State Key Laboratory of Applied Microbiology Southern China&Institute of Microbiology Guangdong Academy of SciencesGuangzhou 510070China State Key Laboratory of Food Science and Technology&National Engineering Research Center for Functional Foods&Synergetic Innovation Center of Food Safety&Joint International Research Laboratory on Food Safety School of Food Science and TechnologyJiangnan UniversityWuxi 214122China

Rapid,sensitive,point-of-care detection of pathogenic bacteria is important for food *** this study,we developed a novel quantum dot nanobeads-labelled lateral flow immunoassay strip(QBs-labelled LFIAS)combined with strand displacement loop-mediated isothermal amplification(SD-LAMP)for quantitative Salmonella Typhimurium(ST)*** dot nanobeads(QBs)served as fluorescence reporters,providing good detection *** customizable strand displacement(SD)probe was used in LAMP to improve the specificity of the method and prevent by-product *** was based on a sandwich immunoassay.A fluorescence strip reader measured the fluorescence intensity(FI)of the test(T)line and control(C)*** linear detection range of the strip was 10^(2)–10^(8) colony forming units(CFU)·mL^(-1).The visual limit of detection was 10^(3) CFU·mL^(-1),indicating that the system was ten-fold more sensitive than AuNPs-labelled test *** specificity was analyzed in accordance with agarose gel outputs of polymerase chain reaction(PCR)and *** detected ST in foods with an acceptable recovery of 85%–110%.The method is rapid,simple,almost equipment-free,and suitable for bacterial detection in foods and for clinical diagnosis.

关键词： Salmonella Typhimurium Quantum dot nanobeads Lateral flow immunoassay strip Loop-mediated isothermal amplification Strand displacement probe

Composition characterization and transformation mechanism of dissolved organic phosphorus in wastewater treatment using 31P NMR spectroscopy

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Frontiers of Environmental Science & Engineering 2024年第3期18卷 103-113页

作者： yuting Zhang Wei shang SoonThiam Khu Xingcan Zheng Yongli Sun Pengfeng Li Miao Gu Wen-an Zhang Huanmei Ma National Engineering Research Center For Urban Water&Wastewater North China Municipal Engineering Design&Research Institute Co.Ltd.Tianjin 300381China School of Environment Science&Engineering Tianjin UniversityTianjin 300350China

The migration and transformation of phosphorus components in wastewater treatment plants(WWTPs)play a crucial role in the convergence and circulation of ***,the composition and variation of dissolved organic phosphorus(DOP)in WWTPs were unclear because of its complex nature,hindering its efficient *** this study,the DOP species and their transformation during the treatment process in WWTP were comprehensively ***,two enrichment methods were assessed for their effectiveness at facilitating wastewater analysis:lyophilization and aluminum salt *** salt precipitation was found to be better because its application allowed 31P nuclear magnetic resonance(31P NMR)spectroscopy to identify more species in the secondary effluent:orthophosphate(Ortho-P)(81.1%–89.3%of the dissolved total phosphorus),pyrophosphates(Pyro-P)(0%–2.3%),orthophosphate monoesters(Mono-P)(7.0%–10.77%),orthophosphate diesters(Di-P)(1.0%–2.96%),and phosphonate(Phos-P)(1.7%–5.16%).Furthermore,the variation and transformation mechanism of phosphorus,particularly those of DOP,during the entire sewage-treatment process were *** the treatment steps,biological treatment combined tertiary treatment achieved better DOP removal ***,biological treatment mainly removed Mono-P and Di-P with removal efficiencies of 33.3%and 41.7%compared with the effluent of the grit ***-P has higher bioavailability and is more easily converted and utilized by microorganisms than ***,Phos-P,with low bioavailability,was hardly utilized by microorganisms,which showed only 18.4%removal efficiency in biological *** tertiary treatment,coagulation process exhibited higher removal ability of Ortho-P(69.1%)and partial removal efficiencies of DOP,resulting in an increase in the DOP proportion in *** addition,Phos-P could not be effectively removed through the biological treatment and was only partially reduced via the adsorption pro

关键词： Municipal wastewater Dissolved organic phosphorus Composition Transformation 31P nuclear magnetic resonance

On challenges of AI to cognitive security and safety

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Security and Safety 2023年第2期2卷 44-56页

作者： Ruiyang Huang Xiaoqing Zheng yuting shang Xiangyang Xue National Digital Switching System Engineering&Technological R&D Center Zhengzhou 450002China Fudan University Shanghai 200433China

Recent advances in deep learning have led to disruptive breakthroughs in artificial intelligence(AI),fueling the jump in ChatGPT-like large language models(LLMs).As with any emerging technology,it is a two-sided coin,bringing not only vast social impacts but also significant security concerns,especially in the socio-cognitive *** this back-ground,this work starts with an inherent mechanism analysis of cognitive domain games,from which it proceeds to explore the security concerns facing the cognitive domain as well as to analyze the formation mechanisms of a cognitive immune ***,inspired by behavioral mimicry in biology,this work will elaborate on new approaches to cognitive security from three aspects:Mimicry Computing,Mimicry Defense,and Mimicry Intelligence.

关键词： Cognitive security Artificial intelligence Endogenous safety and security Mimicry intelligence

高温、高湿条件下模拟仓储小麦品质与微生物群落结构的变化

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中国食品学报 2022年第4期22卷 265-275页

作者：尚玉婷杨薇张民天津农学院食品科学与生物工程学院天津300392 天津农学院基础科学学院天津300392

研究小麦从入库储藏到发现菌丝体过程中,其品质和微生物群落结构的变化趋势。以天津市实仓储藏的硬质白小麦籽粒为研究对象,以三角瓶为储藏容器,分别在30℃、60%RH,40℃、80%RH条件下对小麦进行模拟仓储,储藏时间为0~120 d。通过监测小... 详细信息

研究小麦从入库储藏到发现菌丝体过程中,其品质和微生物群落结构的变化趋势。以天津市实仓储藏的硬质白小麦籽粒为研究对象,以三角瓶为储藏容器,分别在30℃、60%RH,40℃、80%RH条件下对小麦进行模拟仓储,储藏时间为0~120 d。通过监测小麦籽粒的水分、色差、蛋白质、脂肪、淀粉、脂肪酸等品质指标变化,以及霉菌总数、高通量测序结果评估模拟粮库中小麦在不同储藏阶段的品质与真菌群落结构变化。结果表明,在30℃、60%RH条件下,储藏120 d内,小麦品质呈缓慢下降趋势,80~120 d曲霉属相对丰度迅速增加。在40℃、80%RH条件下,小麦品质劣变更快,在60 d时曲霉属相对丰度达100%,小麦产生可见菌丝体。研究表明随着储藏温度与相对湿度的升高,小麦品质变化速度明显加快,菌丝体产生速度加快。

关键词：小麦模拟仓储高通量测序品质变化

基于AM5728的动态产品姿态获取系统设计与实现

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计算机应用与软件 2023年第2期40卷 112-117页

作者：尚玉廷广州番禺职业技术学院智能制造学院广东广州511487 珠海格力电器股份有限公司珠海格力智能装备有限公司广东珠海519000

为了能够实现智能化柔性生产,需要实时获取动态流水线上产品摆放姿态,并根据产品摆放姿态实时调整机构姿态控制机器人等执行机构以设计好的路径执行相应动作,故设计了动态产品姿态获取系统。基于AM5728控制器设计控制系统硬件平台并进... 详细信息

为了能够实现智能化柔性生产,需要实时获取动态流水线上产品摆放姿态,并根据产品摆放姿态实时调整机构姿态控制机器人等执行机构以设计好的路径执行相应动作,故设计了动态产品姿态获取系统。基于AM5728控制器设计控制系统硬件平台并进行实际测试研究,采用图像增强处理、形态学等给出一种外轮廓精确快速提取算法。在不接触产品任意外观面的前提下实时获取动态流水线上产品摆放姿态,为实现后续的智能化柔性生产提供技术基础和理论支撑。

关键词：动态流水线产品摆放姿态图像处理 AM5728

Oncogenic β-catenin-driven liver cancer is susceptible to methotrexate-mediated disruption of nucleotide synthesis

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Chinese Medical Journal 2024年第2期137卷 181-189页

作者： Fangming Liu yuting Wu Baohui Zhang Shuhui Yang Kezhuo shang Jie Li Pengju Zhang Weiwei Deng Linlin Chen Liang Zheng Xiaochen Gai Hongbing Zhang Department of Physiology State Key Laboratory of Common Mechanism Research for Major DiseasesHaihe Laboratory of Cell EcosystemInstitute of Basic Medical Sciences and School of Basic MedicineChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005China Department of Physiology Institute of Basic Medical Sciences and School of Basic MedicineGraduate School of Peking Union Medical CollegeChinese Academy of Medical SciencesBeijing100005China Department of Physiology School of Life ScienceChina Medical UniversityShenyangLiaoning 110122China School of Life Sciences Westlake UniversityHangzhouZhejiang 310024China Key Laboratory of Pediatric Hematology and Oncology Ministry of HealthInstitute of Pediatric Translational MedicineShanghai Children’s Medical CenterShanghai Jiao Tong School of MedicineShanghai 200127China

Background:Liver cancer is largely resistant to *** study aimed to identify the effective chemotherapeutics forβ-catenin-activated liver cancer which is caused by gain-of-function mutation of catenin beta 1(CTNNB1),the most frequently altered proto-oncogene in hepatic ***:Constitutiveβ-catenin-activated mouse embryonic fibroblasts(MEFs)were established by deleting exon 3(β-catenin^(Δ(ex3)/+)),the most common mutation site in CTNNB1 gene.A screening of 12 widely used chemotherapy drugs was conducted for the ones that selectively inhibitedβ-catenin^(Δ(ex3)/+)but not for wild-type *** metabolomics was carried out to examine the alterations of metabolites in nucleotide *** efficacy and selectivity of methotrexate(MTX)onβ-catenin-activated human liver cancer cells were determined in ***-deficient nude mice subcutaneously inoculated withβ-catenin wild-type or mutant liver cancer cells and hepatitis B virus(HBV);β-catenin^(lox(ex3)/+)mice were used,respectively,to evaluate the efficacy of MTX in the treatment ofβ-catenin mutant liver ***:MTX was identified and validated as a preferential agent against the proliferation and tumor formation ofβ-catenin-activated *** nucleotide synthesis was the major metabolic aberration inβ-catenin-active cells,and this alteration was also the target of ***,MTX abrogated hepatocarcinogenesis of HBV;β-catenin^(lox(ex3)/+)mice,which stimulated concurrent Ctnnb1-activated mutation and HBV infection in liver ***:MTX is a promising chemotherapeutic agent forβ-catenin hyperactive liver *** repurposing MTX has the advantages of lower risk,shorter timelines,and less investment in drug discovery and development,a clinical trial is warranted to test its efficacy in the treatment ofβ-catenin mutant liver cancer.

关键词： Liver cancer β-catenin Methotrexate Nucleotide synthesis Chemotherapy

产业技术创新联盟信息共享决策研究——基于区块链技术的应用

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技术经济 2023年第5期42卷 16-28页

作者：王莉尚宇婷山西财经大学管理科学与工程学院太原030031

将区块链技术应用于产业技术创新联盟信息共享领域,可解决联盟信息共享安全问题,提高联盟主体信息共享意愿,加深信息共享程度,进而提升联盟信息共享效率。使用演化博弈方法,构建产业技术创新联盟企业和学研两方的信息共享博弈模型,并通... 详细信息

将区块链技术应用于产业技术创新联盟信息共享领域,可解决联盟信息共享安全问题,提高联盟主体信息共享意愿,加深信息共享程度,进而提升联盟信息共享效率。使用演化博弈方法,构建产业技术创新联盟企业和学研两方的信息共享博弈模型,并通过Matlab仿真对影响双方信息共享决策的政府与区块奖励、惩罚力度、边际成本、风险系数5项因素的影响结果与演化逻辑进行具体分析。结果表明,与传统产业技术创新联盟信息共享过程相比,区块链技术的应用对其共享过程中的各项因素的关键参数值产生了较大影响,更容易推动双方实现均共享的理想均衡状态,但各因素的影响程度不同。有条件的联盟应致力于区块链技术与联盟信息共享网络的融合工作,根据实际情况制定合适的因素组合协同策略,促进联盟的可持续高速发展。

关键词：产业技术创新联盟区块链演化博弈信息共享

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基于深度学习的粘连米粒实例分割算法研究

中国粮油学报 2022年第9期37卷 224-230页

作者：尚玉婷王粤刘彬浙江工商大学信息与电子工程学院杭州310018

基于机器视觉的农作物外观质量检测近些年越来越受到人们的关注。在抽样检测台上由于米粒可能相互触碰、粘连,采集的图像若不进行分割预处理会造成后续大米外观品质评测的失误。由此,本实验提出了一种基于Mask R-CNN改进的实例分割网络R... 详细信息

基于机器视觉的农作物外观质量检测近些年越来越受到人们的关注。在抽样检测台上由于米粒可能相互触碰、粘连,采集的图像若不进行分割预处理会造成后续大米外观品质评测的失误。由此,本实验提出了一种基于Mask R-CNN改进的实例分割网络RiceInstNet,用于粘连米粒的图像分割。主干网络由2个改进的VoVNetV2网络并行组成,在大规模减少网络参数的同时加强对粘连米粒图像的特征提取,另外在掩膜分支上增加了一个学习物体边界的子网络,利用边界特征丰富掩膜特征并促进掩膜预测得更加精细。实验结果表明,该网络可以实现对粘连米粒的实例分割,与Mask R-CNN网络相比,改进后的网络模型RiceInstNet的平均精度和召回率分别由87.2%、89.7%提升到90.4%、93.7%,同时本网络模型更轻量,非常适合集成到移动终端或嵌入式设备中。

关键词：实例分割轻量级粘连边界子网络深度学习