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Molecular mechanism of lycopene cyclases regulating carotenoids ratio in different branches during tea leaf and flower development

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Horticultural Plant Journal 2023年第6期9卷 1177-1192页

作者： Anqi Peng Xiaoyan Tang Yingying Feng Yun Huang Jilai Cui Kai Tian Mengqian Lu Yifan Zhao yuting pan Sanjin Wang Yutong Hu Wei Zhao Chuankui Song Qiang Wang State Key Laboratory of Tea Plant Biology and Utilization and International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural UniversityAnhuiHefei 230036China Hefei Ecological Environment Monitoring Center AnhuiHefei 230036China College of Life Science Xinyang Normal UniversityXinyangHenan 464000China Henan Field Observation and Research Station of Headwork Wetland Ecosystem of the Central Route of South-to-North Water Diversion Project School of Life Sciences and Agricultural EngineeringNanyang Normal UniversityNanyangHenan 473061China

Carotenoids are essential components in tea quality, contributing to leaf color and aroma. However, little information about carotenoids in different tea cultivars and their biosynthesis regulation mechanism during leaf development is known. Here we analyzed carotenoids by HPLC in the buds and leaves of 113 tea cultivars harvested on the same day. By profile clustering, carotenoids were divided into five groups. Same group cultivars displayed divergence in the total content of carotenoids but a similar molar ratio. To figure out the molecular mechanisms of this phenomenon, we further characterized all functional lycopene cyclases, which are the branch point of the carotenoid biosynthesis pathway. Two β-lycopene cyclases(CsLCYB1 and CsLCYB2) and one ε-lycopene cyclase(CsLCYE1) were cloned. Subcellular localization analysis showed that all cloned CsLCYs were localized in plastids. Enzyme activity assays in E. coli indicated both CsLCYBs catalyzed lycopene into β-carotene, and CsLCYE1 produced δ-carotene and ε-carotene. We found CsLCYB1 and CsLCYE1 predominantly expressed in leaf, while CsLCYB2was mainly expressed during flowering stages. Suppression by antisense oligonucleotides reduced CsLCYB1 and CsLCYE1 transcripts and led to reduction of both β,β-branch and β,ε-branch carotenoids in leaf. The expression levels of CsLCYB1 showed a significant positive correlation withβ,β-branch carotenoids in leaf. Our study provides carotenoid profiles of different tea cultivars, which can assist tea producers in selecting cultivars of interest. Meanwhile, we proposed the molecular mechanism of carotenoids reflecting the tenderness of tea plant leaf from a metabolic flux perspective, and suggested lycopene cyclase that could be applied to the breeding of tea varieties with different branch carotenoids.

关键词： Camellia sinensis Carotenoids Lycopene cyclase Metabolicﬂux Leaf development Flower

Promoter and coding sequence diversity of CsCCD1 may contribute to the differential accumulation of floralβ-ionone in fresh tea leaves

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Horticultural Plant Journal 2023年第4期9卷 837-847页

作者： Jingming Wang Mingyue Zhao Ting Gao Yingying Feng Feiquan Wang yuting pan Jieyang Jin Tingting Jing Mengqian Lu Mengting Zhang Danyang Guo Xiaochun Wan Wilfried Schwab Chuankui Song State Key Laboratory of Tea Plant Biology and Utilization International Joint Laboratory on Tea Chemistry and Health EffectsAnhui Agricultural UniversityHefeiAnhui 230036China Wuyi University WuyishanFujian 354300China Biotechnology of Natural Products Technische Universität MünchenLiesel-Beckmann-Str.1Freising 85354Germany

The carotenoid-derived volatileβ-ionone makes an important contribution to tea fragrance.Here,we qualitatively and quantitatively analysed 15 carotenoids in tea leaves of 13 cultivars by UHPLC-APCI-MS/MS.The 13 cultivars were divided into two groups by PCA(Principal Component Analysis)clustering analysis of their carotenoid content,and OPLS-DA(Orthogonal projections to latent structures)indicated that the levels ofβ-carotene(VIP=2.89)and lutein(VIP=2.30)were responsible for much of the variation between the two groups.Interestingly,theβ-carotene toβ-ionone conversion rates in Group 1 were higher than in Group 2,while theβ-carotene content was significantly lower in Group 1 than in Group 2.Theβ-ionone content was significantly higher in Group 1.Pearson Correlation Coefficient calculation between the transcription level of candidate genes(CsCCD1 and CsCCD4)and the accumulation ofβ-ionone indicated that CsCCD1 may involve in the formation ofβ-ionone in 13 cultivars.Prokaryotic expression and in vitro enzyme activity assays showed that‘Chuanhuang 1’had an amino acid mutation in carotenoid cleavage dioxygenases 1(CsCCD1)compared with‘Shuchazao’,resulting in a significantly higherβ-ionone content in‘Chuanhuang 1’.Sequence analysis showed that‘Chuanhuang 1’and‘Huangdan’had different CsCCD1 promoter sequences,leading to significantly higher CsCCD1 expression andβ-ionone accumulation in‘Chuanhuang 1’.These results indicated that the promoter and coding sequence diversity of CsCCD1 might contribute to the differential accumulation ofβ-ionone in different tea cultivars.

关键词： Camellia sinensis(L.)Kuntze Carotenoid cleavage dioxygenase β-carotene β-ionone Promoter Differential accumulation

The capacity and mechanisms of various oxidants on regulating the redox function of ZVI

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Chinese Chemical Letters 2021年第6期32卷 2066-2072页

作者： Siqi Zou Qun Chen Yang Liu yuting pan Gang Yao Zhicheng pan Bo Lai State Key Laboratory of Hydraulics and Mountain River Engineering College of Architecture and EnvironmentSichuan UniversityChengdu 610065China Sino-German Centre for Water and Health Research Sichuan UniversityChengdu 610065China Department of Central Transportation Center West China HospitalSichuan University/West China School of NursingSichuan UniversityChengdu 610065China Institute of Environmental Engineering RWTH Aachen UniversityAachen 52072Germany Sichuan University Yinbin Park Yibin Institute of Industrial TechnologyYibin 644044China Laboratory of Wastewater Treatment Technology in Sichuan Province Haitian Water GroupChengdu 610065China

It is well known that zero-valent iron(ZVI)could catalyze the oxidation of various oxidants to realize the rapid oxidation removal of pollutants.However,in this study,we found that the addition of different oxidants could regulate the redox function of ZVI system.In three different co-treatment systems,the effects of different oxidizers(peroxymonosulfate(PMS),persulfate(PDS),hydrogen peroxide(H_(2)O_(2)))dosages on the ratios of oxidative degradation rate and reductive degradation rate of p-nitrophenol(PNP)were studied.The effect of the H+released from oxidizers and the generated reactive oxygen species(ROS)in ZVI/PMS,ZVI/PDS,ZVI/H_(2)O_(2)systems were detailed discussed.Especially,the contribution of generated ROS for reductive degradation of PNP was quantified in the ZVI/H_(2)O_(2)system.Based on the results of TOC removal,UV-vis absorption spectra,and intermediates concentration curves,it was found that the degradation of PNP changed from reduction to oxidation with the increase of oxidant proportion.When the molar ratio of ZVI to oxidizer equal to 100,PNP was mainly degraded by reduction accompanied by slight oxidation.Combined with the results of SEM-EDS and XPS,it was confirmed that the enhanced degradation of PNP under the addition of oxidant was mainly related to the generated ROS,the additional H+,and the corrosion products of ZVI.

关键词： Zero-valent iron Oxidizer Redox Mechanisms p-Nitrophenol

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基于双甜点的页岩储层可压性评价方法

深圳大学学报（理工版） 2024年第2期41卷 183-191页

作者：史璨林伯韬谢勃勃潘玉婷张家豪成都理工大学能源学院成都610059 中国石油大学(北京)人工智能学院北京102249 新疆油田工程技术研究院新疆克拉玛依834000

页岩储层的甜点和可压性评价是页岩油气勘探开发的重要评价指标,现场往往对储层甜点和可压性分别进行评价,而页岩储层甜点和可压性的评价参数和数学方法存在相似性,易造成二者概念混淆、相互关系不清和重复评价等问题.为明确二者的联系... 详细信息

页岩储层的甜点和可压性评价是页岩油气勘探开发的重要评价指标,现场往往对储层甜点和可压性分别进行评价,而页岩储层甜点和可压性的评价参数和数学方法存在相似性,易造成二者概念混淆、相互关系不清和重复评价等问题.为明确二者的联系性,建立一致性评价方法,系统调研了国内外常用的甜点和可压性评价方法,从概念和评价方法出发,厘清了地质甜点、工程甜点及可压性的关联性,并基于甜点综合分析提出可压性评价方法.该方法利用现场地质、工程参数计算地质和工程甜度指数,构建储层的可压性指数和区域可压性评价图版,可快捷、定量地评价单井及区块的可压性优劣.通过选用四川盆地威远区块10口页岩气井的地质和工程参数,应用所建方法计算了甜度及可压性,发现可压性指数等比例地正向反映了实际测试产气量.该方法能为优化井眼轨迹、提高优质储层钻遇率和优化储层压裂施工方案提供有效的科学指导.

关键词：石油与天然气页岩储层地质甜点工程甜点可压性综合权重

Volatile compound-mediated plant-plant interactions under stress with the tea plant as a model

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Horticulture Research 2023年第9期10卷 281-292页

作者： Jieyang Jin Mingyue Zhao Tingting Jing Mengting Zhang Mengqian Lu Guomeng Yu Jingming Wang Danyang Guo yuting pan Timothy D.Hoffmann Wilfried Schwab Chuankui Song State Key Laboratory of Tea Plant Biology and Utilization International Joint Laboratory on Tea Chemistry and Health EffectsAnhui Agricultural University230036HefeiAnhuiChina Biotechnology of Natural Products Technische Universität MünchenLiesel-Beckmann-Str.185354 FreisingGermany

Plants respond to environmental stimuli via the release of volatile organic compounds(VOCs),and neighboring plants constantly monitor and respond to these VOCs with great sensitivity and discrimination.This sensing can trigger increased plant fitness and reduce future plant damage through the priming of their own defenses.The defense mechanism in neighboring plants can either be induced by activation of the regulatory or transcriptional machinery,or it can be delayed by the absorption and storage of VOCs for the generation of an appropriate response later.Despite much research,many key questions remain on the role of VOCs in interplant communication and plant fitness.Here we review recent research on the VOCs induced by biotic(i.***.insects and pathogens)and abiotic(i.***.cold,drought,and salt)stresses,and elucidate the biosynthesis of stress-induced VOCs in tea plants.Our focus is on the role of stress-induced VOCs in complex ecological environments.Particularly,the roles of VOCs under abiotic stress are highlighted.Finally,we discuss pertinent questions and future research directions for advancing our understanding of plant interactions via VOCs.

关键词： stress drought compound

The analysis of distributed photovoltaic access system schemes

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International English Education Research 2014年第12期 70-73页

作者： yuting pan Yuchen Chen Zhiqiang Yuan Bo Liu Shanghai University of Engineering Science Songjiang District Shanghai China Shanghai Electric Power Design Institute Co. Ltd. Huangpu District Shanghai China

Cold Stress-induced Glucosyltransferase CsUGT78A15 is Involved in the Formation of Eugenol Glucoside in Camellia sinensis

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Horticultural Plant Journal 2020年第6期6卷 439-449页

作者： Mingyue Zhao Binbin Cai Jieyang Jin Na Zhang Tingting Jing Jingming Wang yuting pan Zixiang Zhou Yifan Zhao Yingying Feng Feng Yu Mengting Zhang Yating Li Zhonghua Liu Chuankui Song State Key Laboratory of Tea Plant Biology and Utilization International Joint Laboratory on Tea Chemistry and Health EffectsAnhui Agricultural UniversityHefei 230036China Key Laboratory of Tea Science of Ministry of Education National Research Center of Engineering Technology for Utilization of Functional Ingredients from BotanicalsCollege of HorticultureHunan Agricultural UniversityChangsha 410128China

Eugenol is a natural phenolic compound known for its health-promoting properties and its ability to add a floral scent to tea plants.Plant eugenol glycosides have been identified and shown to make important contributions to fruit floral quality.However,the details of their biosynthesis and metabolism in tea plants are still unknown.Here,eugenol glucoside was unambiguously identified as a native metabolite in the tea plant,and its biosynthesis was shown to be induced by low temperature treatment.Through the analysis of UGTs induced by low temperature,the glycosyltransferase CsUGT78A15 was identified in tea,and its encoded protein was shown to catalyze the glucosylation of eugenol.Vmax/Km ratios showed that eugenol was the most suitable substrate for CsUGT78A15.Sugar donor preference analysis showed that CsUGT78A15 had a higher selectivity for glucose,followed by galactose and glucuronic acid.The expression of CsUGT78A15was correlatedwith the accumulation of eugenol glucoside in different tissues and genotypes of tea.Down-regulation of CsUGT78A15 led to a decreased eugenol glucoside content under cold stress,indicating that CsUGT78A15 plays an important role in the biosynthesis of eugenol glucoside under cold stress.The identification of eugenol glucoside in the tea plant and the discovery of a cold stress-induced eugenol glucosyltransferase in tea provide the foundation for the improvement of tea flavor under cold stress and the biotechnological production of eugenol glucoside.

关键词： Camellia sinensis Eugenol Glucosyltransferase Cold stress

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评估的力量:推动公共图书馆改革与创新

四川图书馆学报 2024年第2期 19-27页

作者：潘雨亭刘培旺何颖芳李金南开大学商学院天津300071 天津师范大学管理学院天津300387 山东省图书馆济南250100

为进一步明确公共图书馆评估在图书馆事业中所扮演的角色,探究评估如何在公共图书馆改革与创新中发挥作用。文章立足近十年的公共图书馆评估工作,以第六次、第七次评估为切入点,从图书馆改革创新的方向、动力、途径、效能四个方面着手,... 详细信息

为进一步明确公共图书馆评估在图书馆事业中所扮演的角色,探究评估如何在公共图书馆改革与创新中发挥作用。文章立足近十年的公共图书馆评估工作,以第六次、第七次评估为切入点,从图书馆改革创新的方向、动力、途径、效能四个方面着手,论证评估工作在其中贡献的力量。研究认为,评估以一定的标准引领公共文化服务体系建设,推动公共图书馆高质量发展。通过发挥评估倍增器作用,不断增强图书馆改革创新的理念动力、系统动力、标准动力与结果动力,为图书馆建设、管理、资源、服务等方面的改革创新探索具体途径,最终达成图书馆组织管理效能、事业发展效能、行业引领效能和社会服务效能的全面提升。

关键词：公共图书馆图书馆评估图书馆事业改革创新服务效能

Numerical Analysis on Charging Performance of the Macro-Encapsulating Combined Sensible-Latent Heat Storage System with Structural Parameters

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Journal of Thermal Science 2024年第3期33卷 1026-1036页

作者： WANG Wei pan Zhenfei WANG Jingfu WU yuting MA Chongfang Key Laboratory of Enhanced Heat Transfer and Energy Conservation Ministry of EducationCollege of Energy and Power EngineeringBeijing University of TechnologyBeijing100124China Key Laboratory of Heat Transfer and Energy Conversion Beijing MunicipalityCollege of Energy and Power EngineeringBeijing University of TechnologyBeijing100124China

For combined sensible-latent heat storage system(CSLHS)(termed as the hybrid configuration),macro encapsulation can effectively solve the leakage problem of PCMs.However,due to the poor thermal conductivity of PCMs,the charging performance of the hybrid configuration slightly increases compared to the solid structure(with only sensible materials).Meanwhile,the natural convection in the PCMs zone could improve the charging performance.So,how to improve natural convection intensity is a key issue for the CSLHS by macro encapsulating.It is found that adding fins can significantly enhance natural convection and accelerate the melting of PCM.In this paper,we proposed the hybrid configuration with fins built-in by macro encapsulation,and analyzed its charging performance with different fin structural parameters in the PCM zone by CFD simulation.In the case,the sensible heat storage material is high-temperature concrete and the PCM is a low-melting-point mixed molten salt.We analyzed the effects of fin number,fin length and fin thickness on the charging performance of the hybrid configuration respectively.From the result,the charging performance increases with the fin number,but the increase rate gradually decreases.When the fin number is 6,the charging performance increases by 20.18%compared to the situation without fin.The charging performance increases gradually with the fin length.Compared with the hybrid configuration without fin,for each 10 mm increase in fin length,its charging performances increase by 4.09%,5.26%,7.02%,8.77%,11.70%,and 15.79%,respectively.Different from number and length of fins,the effect of thickness on the charging performance is very small.When the fin thickness increased from 1 mm to 4 mm,the charging performance only increased by 2.3%.It indicates that the main reason for the improving the charging performance is to increase the natural convection intensity by dividing the PCM zone through fins.These results show that the charging performance of the CSLHS with macro encapsulation can be im

关键词： macro-encapsulation hybrid configuration natural convection structural parameter charging performance