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Impact of lipoprotein lipase gene polymorphisms on ulcerative colitis

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World Journal of Gastroenterology 2006年第39期12卷 6325-6330页

作者： Toshihito Kosaka Junji Yoshino Kazuo Inui Takao Wakabayashi Kazumu Okushima Takashi Kobayashi Hironao Miyoshi yuta nakamura Shigekazu Hayashi Taizou Shiraishi Masatoshi Watanabe Takayuki Yamamoto Ai Nakahara Takahiko Katoh Department of Internal Medicine Second Teaching Hospital Fujita Health University School of Medicine 3-6-10 Otobashi Nakagawaku Nagoya 454-8509 Japan Department of Gastroenterology Nagoya Ekisaikai Hospital 4-66 Shounencho Nakagawaku Nagoya 454-8502 Japan Department of Pathologic Oncology Mie University Graduate School of Medicine 2-174 Edobashi Tsu Mie 514-8507 Japan School of Engineering Yokohama National University 79-5 Tokiwadai Hodogaya-ku Yokohama 240-8501 Japan Inflammatory bowel disease centre and department of surgery Yokkaichi Social Insulance Hospital 10-8 Hazuyamacho Yokkaichi Mie Japan Department of Public Health Miyazaki Medical College University of Miyazaki 5200 Kihara Miyazaki 889-1692 Japan

AIM： To examine the influence of lipoprotein lipase （LPL） gene polymorphism in ulcerative colitis （UC） patients.METHODS： Peripheral blood was obtained from 131 patients with UC and 106 healthy controls for DNA extraction. We determined LPL gene polymorphisms affecting the enzyme at Ser447stop, as well as Hind Ⅲ and Pvu Ⅱ polymorphisms using PCR techniques. PCR products were characterized by PCR-RFLP and direct sequencing. Polymorphisms were examined for association with clinical features in UC patients. Genotype frequencies for LPL polymorphisms were also compared between UC patients and controls.RESULTS： In patients with onset at age 20 years or younger, C/G and G/G genotypes for Ser447stop polymorphism were more prevalent than C/C genotype （OR = 3.13, 95% CI = 0.95-10.33）. Patients with H^＋/- or H^-/- genotype for Hind Ⅲ polymorphism also were more nu merous than those with H^＋/＋ genotype （OR = 2.51, 95% CI = 0.85-7.45）. In the group with H^＋/＋ genotype for Hind Ⅲ polymorphism, more patients had serum triglyceride concentrations over 150 mg/dL than patients with H^＋/- or H^- genotype （P 〈 0.01, OR = 6.46, 95% CI = 1.39-30.12）. Hypertriglycemia was also more prevalent in patients with P^＋/＋ genotypes for Pvu Ⅱ polymorphism （P 〈 0.05, OR = 3.0, 95% CI = 1.06-8.50）. Genotype frequency for LPL polymorphism did not differ significantly between UC patients and controls.

关键词： Ulcerative colitis Lipoprotein lipase Lipid metabolism Triglyceride

低温渗氮工艺的进展及其对商用纯钛4点弯曲疲劳性能的影响

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热处理技术与装备 2014年第3期35卷 30-30页

作者： Shoichi Kikuchi yuta nakamura Akira Ueno Kei Ameyama

为了提高商业纯钛(CP)的疲劳和摩擦两种性能,开发了低温渗氮工艺。冷轧作为离子渗氮的前处理,能获得细晶粒,加速氮向材料中扩散。用显微维氏硬度计、光学显微镜、扫描电镜(SEM)、X射线衍射(XRD)和背散射电子衍射技术(EBSD)表征冷轧前处... 详细信息

为了提高商业纯钛(CP)的疲劳和摩擦两种性能,开发了低温渗氮工艺。冷轧作为离子渗氮的前处理,能获得细晶粒,加速氮向材料中扩散。用显微维氏硬度计、光学显微镜、扫描电镜(SEM)、X射线衍射(XRD)和背散射电子衍射技术(EBSD)表征冷轧前处理的渗氮样品的显微组织。在大于600℃渗氮的表面上形成钛氮化合物(TiN和Ti2N)。在冷轧预处理的CP钛渗氮后生成的化合物层比单一渗氮的更厚,因此有更高的硬度。低温渗氮(600℃)的试样以应力比R=0.1,室温下做4点弯曲疲劳试验,能抵制晶粒粗化。此外,根据观察显微组织和断裂表面探讨了渗氮CP钛的疲劳断裂机制。

关键词：弯曲疲劳性能渗氮工艺纯钛低温商用维氏硬度计光学显微镜背散射电子

Globoside accelerates the differentiation of dental epithelial cells into ameloblasts

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International Journal of Oral Science 2016年第4期8卷 205-212页

作者： Takashi nakamura yuta Chiba Masahiro Naruse Kan Saito Hidemitsu Harada Satoshi Fukumoto Division of Molecular Pharmacology and Cell Biophysics Department of Oral Biology Tohoku University Graduate School of Dentistry Division of Pediatric Dentistry Department of Oral Health and Development Sciences Tohoku University Graduate School of Dentistry Division of Developmental Biology and Regenerative Medicine Department of Anatomy Iwate Medical University

Tooth crown morphogenesis is tightly regulated by the proliferation and differentiation of dental epithelial cells. Globoside （Gb4）, a globo-series glycosphingolipid, is highly expressed during embryogenesis as well as organogenesis, including tooth development. We previously reported that Gb4 is dominantly expressed in the neutral lipid fraction of dental epithelial cells. However, because its functional role in tooth development remains unknown, we investigated the involvement of Gb4 in dental epithelial cell differentiation. The expression of Gb4 was detected in ameloblasts of postnatal mouse molars and incisors. A cell culture analysis using HAT-7 cells, a rat-derived dental epithelial cell line, revealed that Gb4 did not promote dental epithelial cell proliferation. Interestingly, exogenous administration of Gb4 enhanced the gene expression of enamel extracellular matrix proteins such as ameloblastin, amelogenin, and enamelin in dental epithelial cells as well as in developing tooth germs. Gb4 also induced the expression of TrkB, one of the key receptors required for ameloblast induction in dental epithelial cells. In contrast, Gb4 downregulated the expression of p75, a receptor for neurotrophins （including neurotrophin-4） and a marker of undifferentiated dental epithelial cells. In addition, we found that exogenous administration of Gb4 to dental epithelial cells stimulated the extracellular signal-regulated kinase and p38 mitogen-activated protein kinase signalling pathways. Furthermore, Gb4 induced the expression of epiprofin and Runx2, the positive regulators for ameloblastin gene transcription. Thus, our results suggest that Gb4 contributes to promoting the differentiation of dental epithelial cells into ameloblasts.

关键词： ameloblast differentiation enamel matrix epiprofin glycosphingolipids tooth development

Simultaneous augmentation of muscle and bone by locomomimetism through calcium-PGC-1αsignaling

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Bone Research 2022年第4期10卷 770-783页

作者： Takehito Ono Ryosuke Denda yuta Tsukahara Takashi nakamura Kazuo Okamoto Hiroshi Takayanagi Tomoki Nakashima Department of Cell Signaling Graduate School of Medical and Dental SciencesTokyo Medical and Dental University(TMDU)Yushima 1-5-45Bunkyo-kuTokyo 113-8549Japan Department of Orthopaedic Surgery The Jikei University School of Medicine3-25-8 Nishi-ShimbashiMinato-kuTokyo 105-8461Japan School of Dentistry Tokyo Medical and Dental University(TMDU)Yushima 1-5-45Bunkyo-kuTokyo 113-8549Japan Department of Biochemistry Tokyo Dental CollegeKandamisakicho 2-9-18Chiyoda-kuTokyo 101-0061Japan Department of Osteoimmunology Graduate School of Medicine and Faculty of MedicineThe University of TokyoHongo 7-3-1Bunkyo-kuTokyo 113-0033Japan Department of Immunology Graduate School of Medicine and Faculty of MedicineThe University of TokyoHongo 7-3-1Bunkyo-kuTokyo 113-0033Japan

Impaired locomotion has been extensively studied worldwide because those afflicted with it have a potential risk of becoming *** exercise at times can be an effective remedy for frailty,but exercise therapy cannot be applied in all clinical *** is safer than exercise,but there are no drugs that reinforce both muscle and bone when administered *** medications increase the risk of adverse events;thus,there is a need for individual drugs targeting both *** this end,we established a novel sequential drug screening system and identified an aminoindazole derivative,locamidazole(LAMZ),which promotes both myogenesis and osteoblastogenesis while suppressing *** of this drug enhanced locomotor function,with muscle and bone significantly ***,LAMZ induced Mef2c and PGC-1αin a calcium signaling–dependent *** this signaling is activated upon physical exercise,LAMZ mimics physical ***,LAMZ is a promising therapeutic drug for locomotor diseases,including sarcopenia and osteoporosis.

关键词： drugs clinical diseases

Effects of docosahexaenoic acid or arachidonic acid supplementation on gene expression and contractile force of rat cardiomyocytes in primary culture

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BIOCELL 2021年第5期45卷 1213-1229页

作者： MIZUNA YANO yuta UMEHARA TOMOKAZU KUDO TAKAO nakamura TADASHI KOSAWADA ATSUYOSHI NISHINA MASAKI SAZUKA DAISUKE SATO ZHONGGANG FENG Graduate School of Science and Engineering Yamagata UniversityYonezawa9928510Japan Graduate School of Medical Science Yamagata UniversityYamagata9909585Japan Department of Materials and Applied Chemistry College of Science and TechnologyNihon UniversityTokyo1018308Japan Department of Health and Nutrition Yamagata Prefectural Yonezawa University of Nutrition SciencesYonezawa9920025Japan

While fatty acids play essential roles in the physiology of the myocardium,conventional culture media contain little *** previously revealed that rat neonatal myocardium mainly contains docosahexaenoic(DHA),linoleic(LA),and arachidonic(AA)acids as polyunsaturated fatty acids(PUFAs),and these contents in cultured cardiomyocytes derived from fetal rats were markedly lower than those in the neonatal *** this study,we first assessed the effects of supplementation of DHA,LA,or AA on the fatty acid contents and the percentage change of contractile area in primarily cultured rat *** on this assessment,we then evaluated the effects of DHA or AA supplementation on mRNA expression and further directly measured the contractile force of cardiomyocytes with the *** study revealed that percentage change of contractile area was maximized under 20μM DHA or 50μM AA supplementation while LA supplementation did not affect this contraction index,and that a widespread upregulation tendency of the mRNA expression related to differentiation,maturity,fatty acid metabolism,and cell adhesion was seen in the cultured cardiomyocytes with supplementation of DHA or *** particular,upregulation of the gene expression of cellular adhesion molecules connexin43 and N-cadherin were remarkable,whereas the effects on differentiation and maturation were less ***,the increase of the percentage change of the contractile area of cardiomyocyte clusters in culture dishes with the supplementations was significant,whereas the enhancement of the contractile force was *** results suggest that supplementation of DHA or AA to the fetal cardiomyocyte culture may play effective roles in preventing the de-differentiation of the cardiomyocytes in culture and that the enhancement of the contractile performance may be mainly attributed to the improvement of intercellular connection.

关键词： Cardiomyocyte culture Polyunsaturated fatty acid mRNA expression Contractile force Intercellular connection

The Effects of Blue LED Light on Behavior and Retinal Function in Maternal and Offspring Mice

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Journal of Behavioral and Brain Science 2017年第8期7卷 348-359页

作者： Megumi Horibe yuta Yoshino Shinichi Domoto Maho nakamura Masamitsu Shimazawa Hideaki Hara Molecular Pharmacology Department of Biofunctional Evaluation Gifu Pharmaceutical University Gifu Japan

In the present study, we investigated whether blue light emission diode (LED) light exposure affects the maternal behavior of mice. The brain function of the offspring mice, including short-term memory, locomotor activity, anxiety-like behavior, and depression-like behavior, was evaluated. Pregnant mice at day 11 were housed in the apparatus for exposure to blue LED light during the daytime. Nesting behavior and the survival of pups were observed until weaning. After weaning, the offspring mice were bred in normal light conditions until 12 weeks old, and then the Y-maze test, open field test, and tail suspension test were performed. Retinal functions were evaluated by electroretinogram and histological analysis. Blue LED light exposure during the daytime induced retinal damage, but did not affect behavior related to maternal care in maternal mice. In the offspring mice, blue LED light exposure during the daytime did not affect the retina or brain functions. These findings suggest that blue LED light during the daytime might not be a risk factor for disruption of the mother-infant relationship or offspring brain development in mice.

关键词： Blue LED Light Maternal Behavior Daytime Retina Brain

Inhibitory Effect of Cigarette Smoke Extract on Experimental Lung Metastasis of Mouse Melanoma by Suppressing Tumor Invasion

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Pharmacology & Pharmacy 2012年第3期3卷 316-321页

作者： yuta Takahashi Shizuyo Horiyama Yoko Kimoto Noriko Yoshikawa Masaru Kunitomo Satomi Kagota Kazumasa Shinozuka Kazuki nakamura Analytical Center School of Pharmacy and Pharmaceutical Sciences Mukogawa Women’s University Nishinomiya Japan Department of Pharmacology School of Pharmacy and Pharmaceutical Sciences Mukogawa Women’s University Nishinomiya Japan Institute for Biosciences Mukogawa Women’s University Nishinomiya Japan

We investigated the effect of a nicotine-and tar-free cigarette smoke extract (CSE) using an experimental metastasis mouse model which was intravenously injected with B16-BL6 mouse melanoma cells. Three-hour pretreatment of cells with various concentrations of CSE (0, 0.1, 0.3, and 1%) dose-dependently reduced the number of lung metastatic nodules 14 days after tumor injection. To elucidate the mechanism of this anti-metastatic effect of CSE, we examined the invasion and migration activities of B16-BL6 cells pretreated with CSE for three hours in vitro. CSE significantly reduced the invasion of cells at 1% and the migration at 0.3% and 1%. Under the same pretreatment conditions, CSE had no effect on the proliferation of cells. These findings suggest that CSE contains some ingredients that suppress hematogenic lung metastasis via inhibition of the invasion and migration activities of mouse melanoma cells.

关键词： Cigarette Smoke Extract (CSE) Anti-Metastasis B16-BL6 Mouse Melanoma Cells Invasion Migration

Successful treatment of conversion chemotherapy for initially unresectable synchronous colorectal liver metastasis

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World Journal of Gastroenterology 2015年第6期21卷 1982-1988页

作者： Kenta Baba Akihiko Oshita Mohei Kohyama Satoshi Inoue yuta Kuroo Takuro Yamaguchi Hiroyuki nakamura Yoichi Sugiyama Tatsuya Tazaki Masaru Sasaki Yuji Imamura yutaka Daimaru Hideki Ohdan Atsushi Nakamitsu Department of Surgery JA Hiroshima General Hospital Department of Gastroenterological and Transplant Surgery Applied Life Sciences Institute of Biomedical and Health Sciences Hiroshima University Department of Pathology JA Hiroshima General Hospital

A 72-year-old woman with a sigmoid colon cancer and a synchronous colorectal liver metastasis(CRLM),which involved the right hepatic vein(RHV)and the inferior vena cava(IVC),was referred to our *** metastatic lesion was diagnosed as initially unresectable because of its invasion into the confluence of the RHV and *** she had undergone laparoscopic sigmoidectomy for the original tumor,she consequently had 3 courses of modified 5-fluorouracil,leucovorin,and oxaliplatin(m FOLFOX6)plus *** tomography revealed a partial response,and the confluence of the RHV and IVC got free from cancer *** 3 additional courses of m FOLFOX6 plus cetuximab,preoperative percutaneous transhepatic portal vein embolization(PTPE)was performed to secure the future remnant liver ***,a right hemihepatectomy was *** postoperative course was *** patient was discharged from the hospital on postoperative day *** had neither local recurrence nor distant metastasis 18 mo after the last surgical *** multidisciplinary strategy,consisting of conversion chemotherapy using FOLFOX plus cetuximab and PTPE,could contribute in facilitating curative hepatic resection for initially unresectable CRLM.

关键词： Initial y unresectable Colorectal liver metastasis