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Long-term dexamethasone treatment diminishes store-operated Ca^(2+) entry in salivary acinar cells

International Journal of Oral Science 2019年第1期11卷 25-32页

作者： Yuichiro Kusuda yusuke kondo Yuta Miyagi Takashi Munemasa yusuke Hori Fumiko Aonuma Shintaro Tsuka Taro Mukaibo Chihiro Masaki Ryuji Hosokawa Division of Oral Reconstruction and Rehabilitation Kyushu Dental University

Corticosteroids are used in the treatment of many diseases; however, they also induce various side effects. Dexamethasone is one of the most potent corticosteroids, and it has been reported to induce the side effect of impaired salivary gland function. This study aimed to evaluate the effects of dexamethasone on mouse submandibular gland function to gain insight into the mechanism of dexamethasone-induced salivary hypofunction. The muscarinic agonist carbachol(CCh) induced salivary secretion and was not affected by short-term dexamethasone treatment but was decreased following long-term dexamethasone administration. The expression levels of the membrane proteins Na^+-K^+-2 Cl-cotransporter, transmembrane member 16 A, and aquaporin 5 were comparable between the control and long-term dexamethasone treatment groups. The CCh-induced increase in calcium concentration was significantly lower in the presence of extracellular Ca^(2+) in the long-term dexamethasone treatment group compared to that in the control group. Furthermore, CCh-induced salivation in the absence of extracellular Ca^(2+) and Ca^(2+) ionophore A23187-induced salivation was comparable between the control and long-term dexamethasone treatment ***, salivation induced by the Ca^(2+) -ATPase inhibitor thapsigargin was diminished in the long-term dexamethasone treatment group. In summary, these results demonstrate that short-term dexamethasone treatment did not impair salivary gland function, whereas long-term dexamethasone treatment diminished store-operated Ca^(2+) entry, resulting in hyposalivation in mouse submandibular glands.

关键词： Corticosteroids carbachol(CCh)induced

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Stress Distribution in Maxillary Central Incisors without Ferrules: A Finite Element Analysis of Post and Core Systems

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Open Journal of Stomatology 2021年第7期11卷 251-262页

作者： Akihiro Tagahara Chihiro Masaki Tomotaka Nodai Takashi Munemasa Taro Mukaibo yusuke kondo Ryuji Hosokawa Division of Oral Reconstruction and Rehabilitation Kyushu Dental University Kitakyushu Japan

Purpose: The purpose of this study was to identify optimal post and core materials for central incisors without ferrules using three-dimensional finite element analysis and three-point bending tests. Methods: Stress analyses were performed with six models: cast metal post and core (MP), composite resin core alone, straight fiber-reinforced post-composite resin core (FSR), tapered fiber-reinforced post-composite resin core, straight titanium post-composite resin core (TSR), and tapered titanium post-composite resin core (TTR). A 100-N load was applied to the lingual surface at a 45° angle to the long axis of the tooth. Maximum von Mises stress distributions were calculated with finite element analysis software. Five samples each of composite resin, straight fiber-reinforced post, straight titanium post, straight fiber-reinforced post and composite resin, and straight titanium post and composite resin were subjected to three-point bending tests, followed by analysis of variance and Tukey’s multiple comparison test. Results: Stress distribution was optimal on TTR. Maximum von Mises stress on the cervical side of the post was greatest in TSR (693 MPa) and TTR (556 MPa). Maximum stress on the apical side of the post was greatest in MP (110 MPa). Maximum stress in surrounding dentin was lowest in MP (203 MPa) and TTR (250 MPa). Gap distance was smallest in MP (0.09 mm) and largest in FSR (0.26 mm). Mean maximum three-point bending force was lowest in composite resin (26.9 N/mm) and highest in titanium post and composite resin (97.1 N/mm). Titanium post bending strength was consistently greater than that of the fiber-reinforced post (p Conclusion: These results revealed optimal stress distribution and high bending strength with the tapered titanium post and resin combination, suggesting that this combination can most effectively prevent root or post fracture in an anterior tooth without a ferr

关键词： Fiber Post Titanium Post Ferrule Three-Dimensional Finite Element Analysis Three-Point Bending Test

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Thermal influence of saliva secretion <i>ex vivo</i>in the mouse submandibular gland

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Open Journal of Stomatology 2013年第1期3卷 83-88页

作者： Taro Mukaibo Tetsuji Nakamoto yusuke kondo Manami Kidokoro Atsushi Imamura Chihiro Masaki Ryuji Hosokawa Department of Oral Reconstruction and Rehabilitation Kyushu Dental University Kitakyushu Japan

The physiological and pharmacological responses of an ex vivo mouse submandibular gland were used to study fluid secretion and cell signaling in response to muscarinic stimulation at increasing temperatures. Saliva production at 37°C was 5.5-fold that at 25°C with pilocarpine stimulation and 9.8-fold that at 25°C with cevimeline stimulation. Both of these muscarinic agonists are used clinically. With the experimental agonist carbachol (CCh), saliva secretion was increased with an increase in temperature, but the CCh concentration producing the peak flow was the same in both dose-response curves, suggesting that the muscarinic receptor itself is not responsible for the temperature dependence. Purinergic agonists also induced temperature-dependent saliva production ex vivo. The calcium ionophore A23187 failed to have a significant effect on saliva production. The CCh-induced increase in intracellular Ca2+ also upregulated the initial increase and sustained the plateau phase of saliva flow. Thus, muscarinic receptor stimulation of saliva production is temperature sensitive due to an increase in intracellular Ca2+.

关键词： Submandibular Gland Fluid Secretion Cevimeline Pilocarpine Thermal Influence

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Effects of Beverage Ingredients on Salivary Fluid Secretion with an <i>ex Vivo</i>Submandibular Gland Perfusion System: Tannic Acid as a Key Component for the Inhibition of Saliva Secretion

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Open Journal of Stomatology 2015年第1期5卷 12-18页

作者： Atsushi Imamura Tetsuji Nakamoto Taro Mukaibo Takashi Munemasa yusuke kondo Manami Kidokoro Chihiro Masaki Ryuji Hosokawa Department of Oral Reconstruction and Rehabilitation Kyushu Dental University Kitakyushu Japan

Tannic acid (TA) and TA containing beverage have been proved to inhibit Ca2+-activated Cl- channel located apical membrane of the secretory cells. However, their effect on salivary fluid secretion is not well investigated. We used mouse ex Vivo submandibular gland perfusion technique to identify the general effect of TA and related beverage samples on muscarinic agonist carbachol induced fluid secretion. Green tea inhibited fluid secretion by 64% from the control, where oolong tea was by 53%, and red wine by 43% which was linked with their TA concentration. On the other hand, though TA was contained at 4.7 μM in white wine sample and 33 μM in coffee extract, no adverse effect was observed. In addition, coffee induced salivation in the absence of carbachol. TA had a negative effect on fluid secretion with a concentration dependent manner. The effects of TA on carbachol induced calcium increase showed identical as fluid secretion, which was initially no effect, and then gradually decreased over the time. These results demonstrate that TA directly inhibits the salivary fluid secretion and it affects not only Ca2+-activated Cl- channel but also intracellular Ca2+ increasing mechanisms.

关键词： Saliva Secretion Red Wine Tannic Acid Ca2+ Activated Cl- Channel

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糖尿病和肥胖症动物模型肌肉及脂肪组织的葡萄糖摄取:利用18F-FDG评价胰岛素和β3受体激动剂的作用

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中华核医学与分子影像杂志 2018年第1期38卷 73-73页

作者：唐军 Seigo Ishino Taku Sugita yusuke kondo Mika Okai Kazue Tsuchimori Masanori Watanabe Ikuo Mori Masaki Hosoya Takashi Horiguchi Hidenori Kamiguchi 上海交通大学医学院附属苏州九龙医院核医学科 Integrated Technology Research Laboratories Pharmaceutical Research Division Takeda Pharmaceutical Company Limited

目的糖尿病和肥胖症的主要原因是胰岛素功能不足导致肌肉和脂肪组织内的糖摄取、糖代谢异常。因此,许多药物的研发都是基于促进肌肉和脂肪组织对葡萄糖的摄取。现已可通过测定血液相关参数来评价上述组织糖代谢的增加,但这仅仅反映了用... 详细信息

目的糖尿病和肥胖症的主要原因是胰岛素功能不足导致肌肉和脂肪组织内的糖摄取、糖代谢异常。因此,许多药物的研发都是基于促进肌肉和脂肪组织对葡萄糖的摄取。现已可通过测定血液相关参数来评价上述组织糖代谢的增加,但这仅仅反映了用药后的全身反应。课题组进行了数项研究来调查药理实验中疾病动物模型的不同肌肉和脂肪组织葡萄糖摄取测定的实用性。方法对肥胖大鼠模型(2型糖尿病模型)、饮食诱导的肥胖鼠模型(肥胖模型)和相应的对照组模型分别注射18F-脱氧葡萄糖(FDG),各模型肌肉和脂肪组织(白色和棕色脂肪)的基础葡萄糖摄取通过生物分布法进行比较。另外,可促进肌肉和脂肪组织葡萄糖摄取的胰岛素和β3受体激动剂(CL316,243)亦用于各模型来评价两者的作用。18F-FDG在各组织的摄取通过测量各组织放射性的方法进行,而18F-FDG的分布采用放射自显影的方法来分析。结果2型糖尿病模型与对照组(瘦大鼠)相比,所有组织的测量结果均显示其基础葡萄糖摄取水平降低。肥胖模型的白色脂肪组织也有相同表现,但肥胖模型棕色脂肪组织的基础葡萄糖摄取水平增加。胰岛素可促进2型糖尿病模型和瘦大鼠模型的葡萄糖摄取,尽管前者的葡萄糖摄取增加有所抑制。同样的现象也存在于对照组,但使用胰岛素后肥胖模型的肌肉和棕色脂肪组织没有观察到葡萄糖摄取的明显增加。2型糖尿病模型与瘦大鼠使用β3受体激动剂后可观察到与使用胰岛素后一样的反应,但前者脂肪组织的葡萄糖摄取增加受抑。结论通过18F-FDG监测组织葡萄糖摄取水平能够观察到疾病动物模型和相应对照模型间葡萄糖摄取的差别,同时可识别各组织对胰岛素和β3受体激动剂的反应。总之,18F-FDG生物分布法可在疾病动物模型上用于抗糖尿病和抗肥胖药物的评价研究。

关键词： 18F-FDG 肥胖大鼠模型饮食诱导的肥胖鼠模型胰岛素 β3受体激动剂

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Induction heating for desorption of surface contamination for high-repetition laser-driven carbon-ion acceleration

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Matter and Radiation at Extremes 2023年第5期8卷 19-25页

作者： Sadaoki Kojima Tatsuhiko Miyatake Hironao Sakaki Hiroyoshi Kuroki yusuke Shimizu Hisanori Harada Norihiro Inoue Thanh Hung Dinh Masayasu Hata Noboru Hasegawa Michiaki Mori Masahiko Ishino Mamiko Nishiuchi Kotaro kondo Masaharu Nishikino Masaki Kando Toshiyuki Shirai Kiminori kondo Kansai Institute for Photon Science National Institutes for Quantum Science and Technology(QST)8-1-7 UmemidaiKizugawaKyoto 619-0215Japan Interdisciplinary Graduate School of Engineering Sciences Kyushu University6-1Kasuga-KoenKasugaFukuoka 816-8580Japan Hitachi Zosen Corporation 7-89 Nanko-Kita 1-chomeSuminoe-kuOsaka 559-8559Japan Department of Accelerator and Medical Physics Institute for Radiological ScienceNational Institutes for Quantum Science and Technology(QST)4-9-1 AnagawaInage-kuChiba 263-8555Japan

This study reports the first experimental demonstration of surface contamination cleaning from a high-repetition supply of thin-tape targets for laser-driven carbon-ion *** adsorption of contaminants containing protons,mainly water vapor and hydrocarbons,on the surface of materials exposed to low vacuum(>10^(−3)Pa)suppresses carbon-ion *** newly developed contamination cleaner heats a 5-μm-thick nickel tape to over 400℃in 100 ms by induction *** the future,this heating method could be scaled to laserdriven carbon-ion acceleration at rates beyond 10 *** contaminant hydrogen is eliminated from the heated nickel surface,and a carbon source layer—derived from the contaminant carbon—is spontaneously formed by the catalytic effect of *** species of ions accelerated from the nickel film heated to various temperatures have been observed *** the nickel film is heated beyond∼150℃,the proton signal considerably decreases,with a remarkable increase in the number and energy of carbon *** Langmuir adsorption model adequately explains the temperature dependence of desorption and re-adsorption of the adsorbed molecules on a heated target surface,and the temperature required for proton-free carbon-ion acceleration can be estimated.

关键词： acceleration heated nickel

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