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Evaluation of in vitro and in vivo immunostimulatory activities of poly(lactic-co-glycolic acid) nanoparticles loaded with soluble and autoclaved Leishmania infantum antigens: A novel vaccine candidate against visceral leishmaniasis

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Asian Pacific Journal of Tropical Medicine 2019年第8期12卷 353-364页

作者： Emrah Sefik Abamor Adil Allahverdiyev ozlem Ayse Tosyali Melahat Bagirova Tayfun Acar Zeynep Mustafaeva Serap Derman Yildiz Technical University Chemical and Metallurgical Engineering FacultyBioengineering Department34220Esenler-IstanbulTurkey Beykent University Faculty of Engineering and ArchitectureBiomedical Engineering Department34398Sariyer-istanbulTurkey

Objective: To prepare and characterize poly lactic-co-glycolic acid(PLGA) nanoparticles loaded with soluble leishmanial antigen or autoclaved leishmanial antigen and explore in vitro and in vivo immunogenicity of antigen encapsulated nanoparticles. Methods: Water/oil/water double emulsion technique was employed to synthesize PLGA nanoparticles, and scanning electron microscopy, Fourier transform infrared spectroscopy and Zeta-potential measurements were used to identify the characteristics of nanoparticles. Cytotoxicity of synthetized nanoparticles on J774 macrophage were investigated by MTT assays. To determine the in vitro immunostimulatory efficacies of nanoparticles, griess reaction and ELISA was used to measure the amounts of NO and cytokines. During the in vivo analysis, Balb/c mice were immunized with vaccine formulations, and protective properties of nanoparticles were measured by Leishman Donovan unit in the liver following the infection. Cytokine levels in spleens of mice were determined by ELISA. Results: MTT assay showed that neither soluble leishmanial antigen nor autoclaved leishmanial antigen encapsulated nanoparticles showed cytotoxicity against J774 macrophage cells. Contrary to free antigens, both autoclaved leishmanial antigen-nanoparticle and soluble leishmanial antigen-nanoparticle formulations led to a 10 and 16-fold increase in NO amounts by macrophages, respectively. Leishman Donovan unit calculations revealed that soluble leishmanial antigen-nanoparticles and autoclaved leishmanial antigen-nanoparticles yielded 52% and 64% protection against visceral leishmaniasis in mouse models. Besides, in vitro and in vivo tests demonstrated that by increasing IFN-γ and IL-12 levels and inhibiting IL-4 and IL-10 secretions, autoclaved leishmanial antigen-nanoparticles and soluble leishmanial antigennanoparticles triggered Th1 immune response. Conclusions: Both autoclaved leishmanial antigen-nanoparticles and soluble leishmanial antigen-nanoparticles form

关键词： Visceral leishmaniasis Vaccine Delivery Immunostimulant Poly lactic-co-glycolic acid(PLGA) Nanoparticle

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Meta-Herpetic Keratitis and Therapeutic Approach: Case Report

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Open Journal of Ophthalmology 2014年第3期4卷 75-78页

作者： ozlem Sahin Department of Ophthalmology/Uveitis DunyaGoz Hospital Ltd. Ankara Turkey

The purpose of this study is to disclose the steps in the therapeutic approach for meta-herpetic corneal ulcer. Report of a case with anterior segment photography was used. The 6 months of follow-up results of the cas... 详细信息

关键词： Corneal Ulcer Corneal Neovascularization Herpes Simplex Virus-1 Meta-Herpetic Keratitis

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Effects of astaxanthin on antioxidant parameters in ARPE-19 cells on oxidative stress model

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International Journal of Ophthalmology(English edition) 2019年第6期12卷 930-935页

作者： Yigit Musa Günes Alime Uguz Cihangir Yalcin Tok ozlem Tok Levent Oz Ahmi Naziroglu Mustafa Department of Ophthalmology Süleyman Demirel University Research and Education HospitalCünür-Isparta 32200Turkey Department of Biophysics Faculty of MedicineSüleyman Demirel University Research and Education HospitalCünür-Isparta 32200Turkey

AIM: To observe the protective effect of astaxanthin(AST) against hydroquinone(HQ) mediated cell death in the apoptotic cascade and evaluate intracellular Ca2+ release, caspase-3, and-9 activation, reactive oxygen species(ROS) production in ARPE-19 ***: We cultured ARPE-19 cells in special mediums and performed MTT tests to determine protective effect of AST, before exposing the cells to HQ in an incubator. We analyzed intracellular Ca2+ release experiments, mitochondrial membrane depolarization, glutathione(GSH), glutathione peroxidase(GSH-Px) and ROS experiments, and apoptosis ***: ROS production ranges depend on the amount of cell death. We computed the correlation between ROS ranges and cell death by 20,70-dichlorofluorescein fluorescence, and Ca2+ levels by Fura-2-AM. HQ-induced cell death found out to rise ranges of caspase-3 and-9, and mitochondrial depolarization. These three steps were delayed by AST ***: ARPE-19 cells are avoided from HQinduced ROS production and caspase-3 and-9 activation by AST. AST may limit the range of caspase synthesis, Ca2+ release and excess production of ROS with antiapoptotic effect. This study proposes a new therapeutic approach for the treatment of age-related macular degeneration.

关键词： apoptosis ARPE-19 cell astaxanthin oxidative stress

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Petroselinum crispum extract ameliorates scopolamine-induced cognitive dysfunction:role on apoptosis,inflammation and oxidative stress

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Food Science and Human Wellness 2022年第5期11卷 1290-1298页

作者： GökselŞener Gözde Karakadıoglu Dilek Ozbeyli Seren Ede Refiye Yanardag ozlem Sacan Asli Aykac Department of Pharmacology Faculty of PharmacyFenerbahce UniversityIstanbul 34758Turkey Department of Pharmacology Institude of Health SciencesMarmara UniversityIstanbul 34854Turkey Department of Medical Pathology Techniques Vocational School of Health ServicesMarmara UniversityIstanbul 34865Turkey Department of Biochemistry Faculty of EngineeringIstanbul Universityş-CerrahpasaIstanbul 34320Turkey Department of Biophysics Faculty of MedicineNear East UniversityNicosia 99138Cyprus

This study was designed to investigate whether Petroselinum crispum(PC)extract has protective effects on the brain in the scopolamine-induced Alzheimer’s disease(AD)rat *** rats were divided into;control,scopolamine(1 mg/kg,i.p.),galantamine(1.5 mg/kg,i.p.)and PC extract(2 g/kg,p.o.)-treated scopolamine *** day 14,the novel object recognition test(NORT)and Morris water maze test(MWMT)were performed and then the rats were ***-induced cognitive impairments observed in the NORT and MWMT,significantly improved with PC extract and galantamine *** reduced M_(1) receptor expression,Bcl-2/Bax ratio,and glutathione levels in the hippocampus and frontal cortex,while malondialdehyde levels,caspase-3/9 expressions,and acetylcholinesterase(AChE)activity were found to be *** the other hand,PC and galantamine treatments reversed these *** conclusion,PC extract has shown an ameliorative effect on the spatial and recognition memory,M_(1) receptor expression,apoptosis,oxidative stress,and increased AChE ***,it was concluded that PC could prevent AD-like conditions and can be used as a functional ***,since animal models do not completely mimic those of humans,based on the data obtained in this study,the importance of PC on human AD should be demonstrated in future studies.

关键词： Petroselinum crispum Apoptosis Spatial memory Recognition memory Oxidative stress M1 receptor

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Expression of Notch pathway components (Numb, Itch, and Siah-1) in colorectal tumors: A clinicopathological study

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World Journal of Gastroenterology 2020年第26期26卷 3814-3833页

作者： Sinem Cil Gonulcu Betul Unal Ibrahim Cumhur Bassorgun Mualla Ozcan Hasan Senol Coskun Gulsum ozlem Elpek Department of Pathology Akdeniz UniversitySchool of MedicineAntalya 07070Turkey Department of Oncology Akdeniz UniversitySchool of MedicineAntalya 07070Turkey

BACKGROUND The role of the Notch pathway in carcinogenesis and tumor progression has been demonstrated in many organs,including the ***,studies aimed at developing therapies targeting this pathway in various cancers require the identification of several factors that may play a role in regulating Notch-1 *** Numb,Itch,and seven in absentia homolog-1(Siah-1)have been shown to contribute to the regulation of Notch signaling,their role in colorectal carcinogenesis and tumor progression has not been fully elucidated to *** To evaluate Numb,Itch,and Siah-1 expression in colorectal tumors to clarify their relationship with Notch-1 expression and their role in carcinogenesis and tumor *** Expression of Notch-1,Numb,Itch,and Siah-1 was investigated in 50 colorectal carcinomas,30 adenomas,and 20 healthy colonic tissues by immunohistochemistry and quantitative real-time polymerase chain reaction(PCR)*** In contrast to Notch-1,which is expressed at higher levels in tumor tissues and adenomas,expression of Numb,Itch,and Siah-1 was stronger and more frequent in normal mucosa(P<0.01).There was a positive correlation between Notch-1 expression and high histological grade,the presence of lymph node metastasis,and advanced-stage tumors,whereas expression of Numb,Itch,and Siah-1 was absent or reduced in tumors with these clinicopathological parameters(P<0.05).In survival analysis,expression of Notch was related to poor prognosis but that of Numb,Itch,and Siah-1 correlated with improved survival(P<0.05).Multivariate analysis revealed Notch-1 expression and loss of Numb expression to be independent prognostic parameters together with lymph node metastasis(P<0.05).CONCLUSION Our findings support the role of Notch-1 in colorectal carcinoma and indicate that loss of Numb,Itch,and Siah-1 expression is associated with *** data also suggest that these three proteins might be involved in the Notch-1 pathway during colo

关键词： Colorectal adenomas Colorectal cancer Notch-1 Numb Itch Siah-1

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Epidemiologic Characteristics of Patients with Cerebral Palsy

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Open Journal of Therapy and Rehabilitation 2014年第3期2卷 126-132页

作者： Fatma Fidan ozlem Baysal Department of Physical Medicine and Rehabilitation Yildirim Beyazit University Faculty of MedicineAnkaraTurkey Department of Physical Medicine and Rehabilitation Inonu University Faculty of MedicineMalatyaTurkey

Aim: The aim of this study was to determine the demographical and clinical features of patients with cerebral palsy (CP) attending to our outpatient or inpatient clinics. Materials and Methods: Children admitted to Inonu University Faculty of Medicine Department of Physical Medicine and Rehabilitation outpatient or inpatient clinics, Malatya, Turkey with the diagnosis of CP during the study period were included in the study. Age, sex, etiological factors, clinical classifications, and epidemiological characteristics such as socio-economic status as well as the problems associated with CP were analyzed in all patients. Results: Of the total of 130 patients with mean ages 51.05 ± 36.06 months, 51 were girls and 79 were boys. The most common etiologic risk factors were bleeding and presence of threatened miscarriage in prenatal period;asphyxia, low birth weight and prematurity in perinatal period;and neonatal convulsions and hyperbilirubinemia in the postnatal period. Consanguineous marriage was present in 24.6% of families. In clinical classification it was seen that 45 subjects were spastic quadriplegic (34.6%), 41 were spastic diplegic (31.5%), 15 were dyskynetic (11.5%), 14 were spastic hemiplegic (10.8%), 10 were mixed type (7.7%) and 5 were hypotonic/ataxic (3.8%). Conclusion: Prenatal risk factors constitute the foremost risk factors in CP etiology. CP incidence can be lowered by close follow up of the newborns who has been considered as high risk newborns due to the presence of risk factors during pregnancies or deliveries, and by increasing the number of neonatal intensive care units. Besides, it is important to raise public awareness about consanguinity marriages in our country.

关键词： Cerebral Palsy Epidemiology Etiology

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Optimum Arrival Routes for Flight Efficiency

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Journal of Power and Energy Engineering 2015年第4期3卷 449-452页

作者： ozlem Sahin Meric Faculty of Aeronautics and Astronautics Eskisehir Turkey

With the development of aircraft equipment, conventional navigation is the shift from performance based navigation (PBN). As is known, conventional navigation is based on ground-based navigation aids;however, PBN is based on aircraft avionics and performance. In this paper, a new method called Point Merge System (PMS) considered as one of PBN procedures will be introduced. PMS has many benefits related to fuel savings and emission reductions by implementing Continuous Descent Approaches (CDAs). A new PMS standard arrival route (STAR) model will be designed in radar simulation and it will be suggested.

关键词： Performance Based Navigation Area Navigation Required Navigation Performance Point Merge System Efficiency

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Echocardiographic Measurement of Pulmonary Arterial Pressure in Patients with Sarcoidosis

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Open Journal of Respiratory Diseases 2016年第1期6卷 7-13页

作者： Ipek Candemir ozlem Ozdemir Kumbasar Ataturk Chest Diseases and Surgery Education and Research Hospital Ankara Turkey Department of Chest Diseases Ankara University Faculty of Medicine Ankara Turkey

Introduction: Pulmonary hypertension (PH) is a significant complication and is poor prognostic factor for sarcoidosis. We evaluated various tests of diagnosis and treatment as well as the associations between PH and value of six-minute walk test (6 MWT) in sarcoidosis patients. Methods: This study was conducted with fifty patients with sarcoidosis. We grouped patients according to echocardiography. Results: PH prevalence in sarcoidosis was found to be 10%. In cases with likely PH, FEV1%, FVC%, MMF% was found to be lower. Although DLCO was less than 80% in the group of likely PH and over 80% in the other, there were no statistical differences between two groups. Mean value of FVC/DLCO ratio was 1.1 ± 0.38 in group of likely PH. There was no significant difference between groups. Mean distance of 6 MWT was 464 ± 105 m in all cases. There were no statistical differences between two groups in 6 MWT. Significant desaturation with exercise was observed in cases with PH (p = 0.007). Conclusion: In all stages, patients who have longer disease duration and abnormal pulmonary function tests, should be examined about PH. Patients should undergo 6 MWT and presence of desaturation after 6 MWT, even without hypoxemia, should be looked after and dealt with accordingly.

关键词： Sarcoidosis PH ECO PFT 6 MWT

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Effect of basic life support training on the knowledge and skills of first aid of first year medical students

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Journal of Acute Disease 2021年第4期10卷 169-172,I0002-I0005页

作者： Esra Karaman Nazire Avcu ozlem Guneysel Department of Emergency Medicine Faculty of MedicineMaltepe UniversityIstanbulTurkey

Objective:To determine the timing of first aid training in the medical school curriculum and the training method with the 8-hour first aid training given to the first-grade students of the faculty of ***:The study was conducted prospectively with 168 first year medical students at Faculty of Medicine,Maltepe University in October *** 8-hour course plan consisting of theoretical and practical applications was *** courses included cardiopulmonary resuscitation,basic life supports,epileptic seizures,heatstroke,aspiration,and drowning issues,while practical applications included cardiopulmonary resuscitation techniques,basic life support scenarios,Heimlich’s Maneuver and the coma *** were sent a link consisting of 17 questions created with Google forms at the beginning and the end of the *** knowledge was measured with the posttest,and pre-and post-training results were ***:A significant increase was found in the rate of correct answers compared to the pre-training *** the rate of correct post-test answers increased significantly in all questions;the increase in the questions related to the subjects supported by practical applications was more *** was found that more incorrect answers were given to questions about environmental ***:It is possible to improve the public recognition of first aid,even with one day of theoretical and practical ***,adding first aid practical courses to the first-year medical school curriculum and raising awareness at an earlier age will play an essential role in medical education.

关键词： Medical education First aid Basic life support Medical school curriculum

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Competitive Advantages and Clustering of SMEs： An Analysis of Clustering Effect on Competition in Small-Scale Enterprises in Istanbul

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Journal of Modern Accounting and Auditing 2011年第9期7卷 994-1003页

作者： Yildiz Yilmaz Guzey ozlem Tasseven Izmir University Turkey Dogus University Turkey

According to Michael Porter＇s Diamond Model, a business cluster is defined as geographic concentration of inter-connected companies and institutions working in a common industry. In addition, clusters encompass an array of collaborating and competing services and providers that create a specialized infrastructure, which supports the cluster＇s industry. The economic cluster model, represents a synergy, a dynamic relationship and a network between not only the companies that comprise a cluster but also the successful partnering of the stakeholders. Today in all over the world, successful clusters have established a greater competitive advantage and wealth creation for their regions when compared to companies not in a cluster. Given this success more policy makers and regions are considering fostering cluster development as building blocks of regional economies. Research is planned and performed for understanding the clustering in SMEs （small and medium enterprises） industrial estate in Istanbul if it gives a competitive advantage to the cluster members or not. It is performed to determine and satisfy the lack of knowledge about the small and medium enterprises＇ issues, technologic systems, production quality standards, capacity usage ratios, interactions with other firms, habits of following the technologic improvements and clustering effects on the members.

关键词： clusters small and medium enterprises (SMEs) clustering

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