检索结果-南通市图书馆

Hantaan Virus Infection Induces both Th1 and ThCTL Immune responses that Associated with the Viral Control and Clinical Outcome in Humans

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hantaan Virus Infection Induces both Th1 and ThCTL Immune re...

第九届全国免疫学学术大会

作者： ying ma yusi zhang bei liu Chunmei zhang Yun zhang Haitao Yu Angang Yang Ran Zhuang boquan jin Department of Immunology the Fourth Military Medical University Department of Infectious Diseases of Tangdu Hospital the Fourth Military Medical University

Background:The potential roles of CD4T cells in viral control have been extensively ***,the contribution of CD4T cells to the host response against Hantaan virus(HTNV)infection remains ***:Here,based on the T-cell epitopes mappaed on HTNV glycoprotein,we studied the effects and characteristics of CD4T cell responses in determining the outcome of hemorrhagic fever with renal ***:A total of 79 novel 15-mer T-cell epitopes on the HTNV glycoprotein were identified,among which 20 peptides were dominant target ***,we showed the presence of both effective Thl responses with polyfunctional cytokine secretion and ThCTL responses with cytotoxic mediators production against HTNV *** HTNV glycoprotein-specific CD4T cell responses inversely correlated with the plasma HTNV RNA load in *** with milder disease outcomes showed broader epitopes targeted and stronger CD4T cell responses against HTNV glycoproteins compared with more severe *** CD4T cells characterized by broader antigenic repertoire, stronger polyfunctional responses,better expansion capacity and highly differentiated effector memory phenotype(CD27CD28CCR7CD45RACD127would elicit greater defense against HTNV infection and lead to much milder outcome of the ***:HTNV glycoprotein-specific CD4T cells could mediate host defense against HTNV infection,maybe through the essential helper for CTLs,inducing antiviral condition of the host cells and cytotoxic effect of *** findings highlight the contribution of CD4T cell-immunity to HTNV control and provide crucial information to better understand the immune defense against HTNV infection.

关键词： Hantaan virus Hemorrhagic fever with renal syndrome Th1 ThCTL Viral load

CD8lowCD100-T cells identifies a novel CD8 T cell subset associated with viral control during human Hantaan virus infection

在线全文

cnki会议

学校读者我要写书评

暂无评论

CD8lowCD100-T cells identifies a novel CD8 T cell subset ass...

第十届全国免疫学学术大会

作者： bei liu ying ma yusi zhang Chunmei zhang jing Yi Ran Zhuang Haitao Yu Angang Yang Yun zhang boquan jin Department of Immunology The Fourth Military Medical University Department of Blood Transfusion Xijing Hospitalthe Fourth Military Medical University Department of Infectious Disease Tangdu Hospital the Fourth Military Medical University

Purpose:Hantaan virus(HTNV)infection can cause a severe lethal hemorrhagic fever with renal syndrome(HFRS)in ***8+T cells play a critical role in combatting HTNV virus ***,the contribution of different CD8+T cell subsets to immune response against viral infection is poorly ***:Plasma and peripheral blood mononuclear cell(PBMC)samples from 68 HFRS patients and 15 healthy controls were *** dynamic change in CD100 expression on CD8+cells,phenotype,functional markers,the viral specific cellular immune response and the relationship between low CD100 expression on CD8+cells and plasma HTNV RNA load during the course of HFRS were ***:We identified a novel subset of CD8+T cells characterized by the CD8lowCD100-phenotype in HFRS *** CD8lowCD100-subset accounted for a median of 14.3%of the total CD8+T cells in early phase of HFRS,and thispercentage was subsequently declined in late phase of infection,whereas this subset was absent in healthy ***,the CD8lowCD100-cells were associated with high activation and expressed high levels of cytolytic effector molecules and exhibited a distinct expression profile of effector/effector memory-like CD8+T cells(CCR7+/-CD45RACD127highCD27intCD28lowCD62L-).When stimulated with specific HTNV-nucleocapsid protein-derived peptide pools,most responding CD8+cells(IFN-γ+and/or TNF-α+)were *** frequency of CD8lowCD100-cells among HTNV-specific CD8+T cells is higher in milder patients than more severe ***,the proportion of the CD8lowCD100-subset among CD8+T cells in early phase of HFRS was negatively correlated with the HTNV viral load,suggesting that CD8lowCD100-cells may be associated with viral *** contraction of the CD8lowCD100-subset in late phase of infection may be related to the consistently highexpression levels of ***:These results may provide new insights into understanding of CD8+T cell-mediat

关键词： HTNV HFRS CD100 CD8+ T cell Viral load

The genetic polymorphisms of HLA are strongly correlated with the disease severity after Hantaan virus infection in the Chinese Han population

在线全文

cnki会议

学校读者我要写书评

暂无评论

The genetic polymorphisms of HLA are strongly correlated wit...

第八届全国免疫学学术大会

作者： ying ma Bin Yuan jing Yi Ran Zhuang Jiuping Wang Yun zhang Zhuwei Xu yusi zhang bei liu Chunmei zhang Angang Yang boquan jin Department of Immunology Xijing Hospital Department of Blood Transfusion Xijing Hospital Department of Infectious Disease Tangdu Hospital the Fourth Military Medical University

Background:Hantaan virus(HTNV),the prototype of the Hantavirus genus,causes severe hemorrhagic fever with renal syndrome(HFRS)in humans with a case fatality rate of approximately 15%.The polymorphism of human leukocyte antigen(HLA),which is considered a genetic factor that influences the progression of HFRS after HTNV infection,was incompletely ***:We aimed to explore the relationship between distinct alleles or haplotypes and the disease progression of HFRS in the Chinese Han ***:In this case-control study,76 HFRS patients and 370 healthy controls from the Chinese Han population at Guanzhong region of Shaanxi province were typed for the HLA-A,-B,and-DRB1 *** regression analysis was carried out for HLA loci,allele or haplotype to estimate their contribution to disease

关键词：

Identification of a novel B-cell epitope of Hantaan virus glycoprotein recognized by neutralizing 3D8 monoclonal antibody

在线全文

cnki会议

学校读者我要写书评

暂无评论

Identification of a novel B-cell epitope of Hantaan virus gl...

第八届全国免疫学学术大会

作者： yusi zhang Guolin Yan ying ma jing Yi bei liu Zhuwei Xu Yun zhang Chunmei zhang Fanglin zhang Zhikai Xu Angang Yang Ran Zhuang boquan jin Department of Immunology Department of Microbiology

Background:Hantaan virus, a member of Bunyaviridae family, is a major agent causing hemorrhagic fever with renal syndrome, a high-mortality-rate disease threatening approximately 150,000 people around the world *** 3G1 and 3D8 m Ab showed a potent virus neutralizing activity both in vitro and in ***, little research has been done on the binding sites of 3G1 and 3D8 m *** far only one epitope 96YPWHTAKCHY105 recognized by 3G1 m Ab has been identified by the phage-displayed peptide *** binding sites of 3D8 m Ab remain ***:In the present study, we mapped the epitopes of GPs recognized by 3D8 m ***:Pepscan methods were carried out to map the epitopes of 3D8 m Ab and the specificities of the binding with the 3D8 m Ab were further confirmed by the competitive ELISA(c ELISA).And then the crucial amino acid residues of the epitope were identified by the alanine-scanning ***:A new B-cell epitope 882GFLCPEFPGSF RKKC896 of HTNV, which locates on Gc, has been screened out from a set of 15-mer synthesized peptides covering the full length of *** has been shown by the alanine-scanning technique that 885 C, 893 R, 894 K, 895 K, and 896 C are the key amino acids of the binding sites of the ***:In summary, the identification of a novel B-cell epitope of GPs recognized by 3D8 m Ab will help us to elucidate the mechanisms of the neutralization activity of 3D8 m Ab and to provide implications for hantavirus vaccinology.

关键词： hantaan virus B-cell epitope neutralizing antibody pepscan method

Sustained Elevation of Serum VEGF is Positively Correlated With Disease Severity of Patients With Hemorrhagic Fever With Renal Syndrome

在线全文

cnki会议

学校读者我要写书评

暂无评论

Sustained Elevation of Serum VEGF is Positively Correlated W...

第八届全国免疫学学术大会

作者： bei liu ying ma Bin Yuan Jiuping Wang Haitao Yu Yun zhang Zhuwei Xu yusi zhang jing Yi Chunmei zhang Xingchu Zhou Angang Yang Ran Zhuang boquan jin Department of Immunology The Fourth Military Medical University Department of Infectious Diseases of Tangdu Hospital The Fourth Military Medical University

Background:Hantaan virus(HTNV), the prototype of the Hantavirus genus, is a rodent-borne pathogen that causes human hemorrhagic fever with renal syndrome(HFRS) in Asia with a mortality rate of approximately 15%.Since hantavirus infections are not lytic, the mechanisms by which hantaviruses cause haemorrhagic fever with renal syndrome(HFRS) are *** increase in permeability of vascular endothelial cells is considered one of the major causes of hemorrhage after Hantaan virus(HTNV) infection in ***:This study aimed to investigate the role of vascular endothelial growth factor(VEGF) in the pathogenesis of endothelial injury in hemorrhagic fever with renal syndrome(HFRS).Method:We detected the levels of VEGF in the serum of HFRS patients and compared the serum levels of VEGF throughout the course of the illness in a large cohort of patients with different *** concentration of VEGF from 197 serum samples of 88 patients was quantified with sandwich *** analysis was performed using SPSS 16.0 software and Prism ***:The level of serum VEGF in HFRS patients was elevated dramatically compared with healthy controls(P maximum till the convalescence ***, the elevated serum VEGF was positively correlated with the level of serum creatinine and the severity of ***:Our results suggested that the sustained elevation of the serum VEGF level is positively correlated with the severity of HFRS, w

关键词： HFRS VEGF ELISA

High-Spin Level Scheme of^(120)I

在线全文

cnki会议

学校读者我要写书评

暂无评论

Chinese Physics Letters 2013年第6期30卷 67-70页

作者： LI Li SUN Ji LI Jian YU De-Yang liu Gong-Ye zhang Wei-Tian ma Ke-Yan YANG Dong ma ying-Jun WU Xiao-Guang ZHU Li-Hua HE Chuang-Ye ZHENG Yun YAO Shun-He LI Cong-Bo HU Shi-Peng CAO Xue-Peng WANG jin-Long YU bei-bei College of Physics Jilin UniversityChangchun 130012 China Institute of Atomic Energy Beijing 102413 School of Physics and Nuclear Energy Engineering Beihang UniversityBeijing 100191

Excited states in^(120)I are investigated utilizing in-beam gamma-ray spectroscopy following the^(114)Cd(^(11)B,5n)reaction at 70 MeV bombarding energy.A level scheme is constructed with states up to 9 *** levels are classified into several bands and their possible configurations at low spins are discussed *** on the linking transitions between different bands at higher spins,the excitation energy of the previously reported T1/2=53 min isomer is ascertained to be 72 *** showing the existence of an isomer at an excitation energy of 6.4 MeV is presented.

关键词： energy bands

Elevated nocturnal and morning blood pressure in patients with obstructive sleep apnea syndrome

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2012年第10期125卷 1740-1746页

作者： HE Quan-ying FENG jing zhang Xilong LIANG Zong-an HUANG Shao-guang KANG Jian WANG Guang-fa zhang Li-qiang ma Li-jun WANG bei LIN Qi-chang zhang jin-nong liu Hui-guo LUO Yuan-ming liu Jian-hong WANG Shi XIAO Gao-hui LU Gan zhang jin FENG Xue-wei CHEN Bao-yuan Department of Respiratory Diseases Peking University People’s HospitalBelling 100044China Department of Respiratory Diseases Tianjin Medical University General HospitalTianiin 300052China Department of Respiratory Diseases First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Department of Respiratory Diseases West China HospitalSichuan UniversityChengduSichuan 610041China Department of Respiratory Diseases Ruijin HospitalShanghai Jiao Tbng University School of MedicineShanghai 200025China Department of Respiratory Diseases First Affiliated Hospita1 ofChina Medical UniversityShenyangLiaoning 110001China Department of Respiratory Diseases Peking University First HospitalBeijing 100034China Department of Respiratory Diseases Peking University Third HospitalBeijing 100191China Department of Respiratory Diseases Henan Provincial People’s HospitalZhengzhouHenan 450003China Department of Respiratory Diseases Second Affiliated Hospita1 of Shanxi Medical UniversityTaiyuanShanxi 030001China Department of Respiratory Diseases First Affiliated Hospital of Fujian Medical UniversityFuzhouFuiian 350005China Department of Respiratory Diseases Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologywuhanHubei 430022China Department of Respiratory Diseases Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430030 China Guangzhou Institute of Respiratory Disease.First Affiliated Hospital of Guangzhou Medical College GuangzhouGuangdong 510120China Department of Respiratory Diseases Guangxi Zhuang Autonomous Region People’s HospitalNanningGuangxi 530021China Department of Respiratory Diseases Central Hospita1 of Shenyang Medical College ShenyangLiaoning 110024China Department of Respiratory Diseases Beijing Gongan HospitalBeijing 100006China Department of Respiratory Diseases Jiangsu Senility HospitalNanjingJiangsu 210024China Department of Respiratory Diseases Afiliated Hospita1 of Ningxi

Background The nocturnal nondipping and elevated morning blood pressure （BP） in patients with obstructive sleep apnea syndrome （OSAS） have not yet been well investigated in Chinese patients. This study aimed to describe the BP profile, and to elucidate the relationships between daytime BP and nighttime BP, and between evening BP and morning BP in patients with OSAS. Methods Twenty teaching hospital sleep centers in China were organized by the Chinese Medical Association to participate in this study and 2297 patients were recruited between January 2004 and April 2006. BP assessments were made at four time points （daytime, evening, nighttime and morning） and polysomnography （PSG） was performed and subjects were classified into four groups by their apnea-hypopnea index （AHI）： control, n=213 with AHI 〈5; mild, n=420 with AHI -〉5 and 〈15; moderate, n=460 with AHI -〉15 and 〈30; and severe, n=1204 with AHI -〉30. SPSS 11.5 software package was used for statistical analysis and figure drawing. Results All the average daytime, nighttime, evening and morning BPs were positively correlated with AHI and negatively correlated with nadir nocturnal oxygen saturation. The ratios of nighttime/daytime and morning/evening average BP were positively correlated with AHI. The ratio of nighttime/daytime systolic BP became a ＂reversed BP dipping＂ pattern until the classification reached severe, while the ratio of nighttime/daytime diastolic BP became reversed at moderate. Similarly, the ratio of morning/evening diastolic BP becomes reversed even at mild. Conclusions OSAS may result in higher BP levels at all four time points. The ratios of nighttime/daytime and morning/evening BP increase with increased AHI. The increasing of diastolic BP, which is inclined to rise more quickly, is not parallel with increasing systolic BP.

关键词： obstructive sleep apnea syndrome blood pressure hypertension, dipping, polysomnography apnea-hypopnea index

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

用于分布式语义查询的语义逼近方法

北京邮电大学学报 2008年第6期31卷 13-17页

作者：马应龙张世鹏刘绍华金蓓弘华北电力大学计算机科学技术学院北京102206 中国科学院软件研究所北京100080 北京邮电大学电子工程学院北京100876

传统的基于关键词的信息查询方法已经不能满足用户的需要.为此,提出基于语义逼近的信息查询方法,探讨了语义替换规则和基于逼近度的查询结果排序策略.最后基于多代理技术实现了一个分布式语义查询系统,能执行跨多个本体的语义查询任务.

关键词：语义逼近语义查询本体多代理系统

Relationship of daytime blood pressure and severity of obstructive sleep apnea among Chinese： a multi-center investigation in China

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2010年第1期000卷 18-22页

作者： HE Quan-ying FENG jing zhang Xi-long LIANG Zong-an HUANG Shao-guang KANG Jian WANG Guang-fa zhang Li-qiang ma Li-jun, WANG bei, LIN Qi-chang, zhang jing-nong, liu Hui-guo, LUO Yuan-ming,liu Jian-hong, WANG Shi, XIAO Gao-hui, LU Gan, zhang jin, FENG Xue-wei and CHEN Bao-yuan ma Li-jun WANG bei LIN Qi-chang zhang jing-nong liu Hui-guo LUO Yuan-ming liu Jian-hong WANG Shi XIAO Gao-hui LU Gan zhang jin FENG Xue-wei CHEN Bao-yuan Department of Respiratory Diseases Beijing University People'sHospital Beijing 100044 China Department ofRespiratory Diseases Tianjin Medical University GeneralHospital Tianjin 300052 China Department of Respiratory Diseases First Affiliated Hospital ofNanjing Medical University Nanjing 210029 China Department of Respiratory Diseases Huaxi Hospital SichuanUniversity Chengdu Sichuan 610041 China Department of Respiratory Diseases Ruijin Hospital S-hanghaiJiao Tong University School of Medicine Shanghai 200025 China Department of Respiratory Diseases First AffiliatedHospital of China Medical University Shenyang Liaoning110001 China Department of Respiratory Diseases Peking University First Hospital Beijing 100034 China Department of Respiratory Diseases Peking University ThirdHospital Beijing 100083 China Department ofRespiratory Diseases Henan Provincial People's HospitalZhengzhou Henan 450003 China Department ofRespiratory Diseases Second Affiliated Hospital of ShanxiMedical University Taiyuan Shanxi 030001 China Department of Respiratory Diseases First Affiliated Hospital ofFujian Medical University Fuzhou Fujian 350005 China Department of Respiratory Diseases Union Hospital TongjiMedical College Huazhong University of Science and TechnologyWuhan Hubei 430022 China Department ofRespiratory Diseases Tongji Hospital Tongji Medical CollegeHuazhong University of Science and Technology Wuhan Hubei430030 China Guangzhou Institute of RespiratoryDisease First Affiliated Hospital of Guangzhou Medical CollegeGuangzhou Guangdong 510120 China Department ofRespiratory Diseases Guangxi Zhuang Autonomous RegionPeople's Hospital Nanning Guangxi 530021 China Department of Respiratory Diseases Central Hospital of ShenyangMedical College Shenyang Liaoning 110024 China Department of Respiratory Diseases Beijing Gongan HospitalBeijing 100006 China Department of RespiratoryDiseases Jiangsu Senility Hospital Nanjing Jiangsu 210024China Department of Respiratory Disea

Background Epidemiologic studies have shown an independent and definite association between obstructive sleep apnea （OSA） and hypertension. This study aimed to define the association between daytime blood pressure and severity of OSA in Chinese population in mainland of China. Methods Twenty university hospital sleep centers in mainland of China were invited by the Chinese Medical Association （Cma） to participate in this epidemiologic study and 2297 consecutive patients （aged 18-85 years; 1981 males and 316 females） referred to these twenty sleep centers for evaluation of OSA between January 2004 and April 2006 were prospectively enrolled. Nocturnal polysomnography was performed in each patient, and disease severity was assessed based on the apnea-hypopnea index （AHI）. These patients were classfied into four groups： non-apneic control （control, n=257） with AHI 〈5 episodes/hour; mild sleep apnea （mild, n=402） with AHI 〉5 and 〈15 episodes/hour; moderate sleep apnea （moderate, n=460） with AHI 〉15 and 〈30 episodes/hour and severe sleep apnea （severe, n=1178） with AHI 〉30 episodes/hour. Daytime blood pressure measurements were performed under standardized conditions in each patient at 10 a.m. in office on the day of referring to sleep centers for getting average value. All the patients were requested to quit medications related to blood pressure for three days before the day of assessing. Results Both daytime systolic blood pressure and diastolic blood pressure values were significantly related to AHI positively （r = 0.201 and 0.276, respectively; both P values 〈0.001） and to nadir nocturnal oxygen saturation negatively （r = -0.215 and -0.277, respectively; both P values 〈0.001）, which were the parameters of OSA severity. In two special designed mean plots, means of daytime systolic and diastolic blood pressure increased gradually with increasing AHI. Beyond AHI of 61-65, this increasing trend reached a plateau. Conclusions The results showed that OSA severity was associ

关键词： obstructive sleep apnea hypertension epidemiology blood pressure polysomnography