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Impact of interlayer on moisture characteristics of reclaimed soil backfilled with Yellow River sediments

International Journal of Agricultural and Biological Engineering 2020年第1期13卷 153-159页

作者： Xiaotong Wang Zhenqi Hu yusheng liang Institute of Land Reclamation and Ecological Restoration China University of Mining and Technology(Beijing)Beijing 100083China China College of Environment Science and Spatial Informatics China University of Mining and TechnologyXuzhou 221116China

Underground coal mining causes land subsidence,and backfilling with Yellow River sediment is an effective reclamation technology to restore farmland in *** date,two-layer soil reconstructed(TSR)for subsided land reclamation resulted in poor capacity to retain *** solve this problem,multi-layered soil reconstructed(MSR),sandwiching soil interlayers between sediment,was developed as a new reclamation strategy with Yellow River *** order to evaluate the impact of soil interlayer on moisture characteristics,laboratory experiments of infiltration and evaporation were *** control treatments(CK1,CK2)and four experimental treatments(T1-T4)were ***1 was undamaged farmland,CK2 was conventional reconstructed two-layers soil profile(filled sediment with 40 cm soil cover).T1-T4 were multiple-layers soil profiles sandwiching different structures of soil interlayers between sediment *** results indicated that putting interlayers into sediment reduced water leakage and water evaporation,improved the water-holding capacity of conventional two-layer soil *** total thickness of soil interlayers of 30 cm(T3 and T4)was better than 20 cm(T1 and T2)and two soil interlayers(T2)were better than one(T1)on water-holding ***,the best reconstructed soil profile was T3,sandwiched two soil interlayer and the first thickness was 20 *** treatment had the greatest improvement on soil water holding capacity with an increase of 49.14%compared to CK2 at the end of the evaporation and was closest to CK1(402.31 mm).This study provided experimental evidence that compares with TSR,MRS improved the moisture characteristics of backfilling with Yellow River sediment.

关键词： land reclamation Mining subsidence Yellow River sediment multi-layered soil reconstructed moisture characteristics

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Effects of Industrial Relocation on Chinese Regional Economic Growth Disparities: Based on System Dynamics Modeling

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Chinese Geographical Science 2014年第6期24卷 706-716页

作者： WU Aizhi LI Guoping SUN Tieshan liang yusheng Library Peking University School of Government Peking University College of Geoscience and Surveying Engineering China University of Mining and Technology

The economic growth of China has led to increasing growth disparities between regions. Such disparities are uncontrolled and are severely negative symptoms in the process of economic development. On the basis of system dynamics(SD) modeling and the relationship between industrial relocation and regional economic growth, we construct a model of the interrelationship between the two aforementioned phenomena. The model is an effective and creative exploration for examining effects of industrial relocation on Chinese regional economic growth disparities. The SD model is employed in this study to build an inter-regional labor migration SD model, an inter-regional capital migration SD model, an intra-industry SD model, an intra-regional population SD model, and an intra-regional SD model which are based on realities in labor and capital flow from the view of industrial relocation. VENSIM software is utilized to perform a system simulation based on the data of the eastern, middle, and western regions from 2000 to 2010. Results show that industrial relocation gradually narrows the relative disparity in GDP among the three regions. Moreover, the absolute one is enlarged continuously. The absolute and relative disparities in per capita GDP among eastern, middle, and western regions generally exhibit decreasing trends.

关键词： industrial relocation economic growth disparity system dynamics (SD) modeling China

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Hepatic metabolomics and transcriptomics to study susceptibility to ketosis in response to prepartal nutritional management

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Journal of Animal Science and Biotechnology 2020年第1期11卷 194-210页

作者： Khuram Shahzad Vincenzo Lopreiato yusheng liang Erminio Trevisi Johan S.Osorio Chuang Xu Juan J.Loor COMSATS Institute of Information Technology ChakShahzadIslamabad 44000Pakistan Department of Animal Sciences and Division of Nutritional Sciences University of IllinoisUrbanaIL 61801USA Istituto di Zootecnica Facoltàdi Scienze AgrarieAlimentari e AmbientaliUniversitàCattolica del Sacro Cuore29122 PiacenzaItaly Department of Dairy Science South Dakota State UniversityBrookingsSD 57006USA College of Animal Science and Veterinary Medicine Heilongjiang Bayi Agricultural UniversityXinyang Rd.5Daqing 163319China.

Background:Ketosis in dairy cows is associated with body fat mobilization during the peripartal ***-clinical and clinical ketosis arise more frequently in cows that are overfed energy during the entire dry(last 50 to 45 days prior to parturition)or close-up period(last^28 days prepartum).Methods:A retrospective analysis was performed on 12 cows from a larger cohort that were fed a higher-energy diet[1.54 Mcal/kg of dry matter(DM);35.9%of DM corn silage and 13%of DM ground corn]during the close-up dry period,of which 6 did not develop clinical ketosis(OVE,0.83 mmol/L plasma hydroxybutyrate;BHB)and 6 were diagnosed with clinical ketosis(KET,1.4 mmol/L BHB)during the first week postpartum.A whole-transcriptome bovine microarray(Agilent Technologies)and metabolomics(GC-MS,LC-MS;Metabolon~?Inc.)were used to perform transcript and metabolite profiling of liver tissue harvested at-10 days relative to parturition which allowed to establish potential associations between prepartal transcriptome/metabolome profiles and susceptibility to clinical ketosis ***:Cows in KET had greater(P=0.01)overall body weight between-2 and 1 week around parturition,but similar body condition score than *** dry matter intake(DMI)did not differ prepartum,KET cows had lower(P<0.01)DMI and similar milk yield as OVE cows during the first week *** analysis revealed a total of 3065 differentially expressed genes(DEG;P≤0.05)in *** identified 15 out of 313 total biochemical compounds significantly affected(P≤0.10)in *** those,greater concentrations(P≤0.06,+2.3-fold)of glycochenodeoxycholate in KET cows also have been detected in humans developing non-alcoholic fatty liver *** analysis using the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway database and the DEG revealed that,among the top 20 most-impacted metabolic pathway categories in KET,65%were overall *** included’Metabolism of cofactors

关键词： Bioinformatics Ketosis Metabolomics Transition cow

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Knockout of butyrophilin subfamily 1 member A1(BTN1A1) alters lipid droplet formation and phospholipid composition in bovine mammary epithelial cells

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Journal of Animal Science and Biotechnology 2020年第4期11卷 975-985页

作者： Liqiang Han Menglu Zhang Zhiyang Xing Danielle N.Coleman yusheng liang Juan J.Loor Guoyu Yang College of Animal Science and Veterinary Medicine Henan Agricultural UniversityZhengzhou 450002PR China Department of Animal Sciences and Division of Nutritional Sciences University of IllinoisUrbanaIllinois 61801USA.

Background: Milk lipids originate from cytoplasmic lipid droplets(LD) that are synthesized and secreted from mammary epithelial cells by a unique membrane-envelopment process. Butyrophilin 1 A1(BTN1 A1) is one of the membrane proteins that surrounds LD, but its role in bovine mammary lipid droplet synthesis and secretion is not well ***: The objective was to knockout BTN1 A1 in bovine mammary epithelial cells(BMEC) via the CRISPR/Cas9 system and evaluate LD formation, abundance of lipogenic enzymes, and content of cell membrane phospholipid(PL) species. Average LD diameter was determined via Oil Red O staining, and profiling of cell membrane phospholipid species via liquid chromatography-tandem mass spectrometry(LC-MS/MS).Results: Lentivirus-mediated infection of the Cas9/sg RNA expression vector into BMEC resulted in production of a homozygous clone BTN1 A1^((-/-)). The LD size and content decreased following BTN1 A1 gene knockout. The m RNA abundance of fatty acid synthase(FASN) and peroxisome proliferator-activated receptor-gamma(PPARG) was downregulated in the BTN1 A1^((-/-))clone. Subcellular analyses indicated that BTN1 A1 and LD were co-localized in the cytoplasm. BTN1 A1 gene knockout increased the percentage of phosphatidylethanolamine(PE) and decreased phosphatidylcholine(PC), which resulted in a lower PC/PE ***: Results suggest that BTN1 A1 plays an important role in regulating LD synthesis via a mechanism involving membrane phospholipid composition.

关键词： Lipid droplet Mammary epithelial cell Milk fat globule Phospholipid

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Multifaceted role of one-carbon metabolism on immunometabolic control and growth during pregnancy, lactation and the neonatal period in dairy cattle

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Journal of Animal Science and Biotechnology 2021年第1期12卷 82-109页

作者： Danielle N.Coleman Abdulrahman S.Alharthi yusheng liang Matheus Gomes Lopes Vincenzo Lopreiato Mario Vailati-Riboni Juan J.Loor Department of Animal Sciences Division of Nutritional SciencesUniversity of IllinoisUrbanaIL 61801USA .2Department of Animal Production College of Food and Agriculture SciencesKing Saud UniversityRiyadh 11451Saudi Arabia .3Department of Animal Sciences Food and NutritionFaculty of AgricultureFood and Environmental ScienceUniversitàCattolica del Sacro Cuore29122 PiacenzaItaly.

Dairy cattle undergo dramatic metabolic, endocrine, physiologic and immune changes during the peripartal period largely due to combined increases in energy requirements for fetal growth and development, milk production, and decreased dry matter intake. The negative nutrient balance that develops results in body fat mobilization,subsequently leading to triacylglycerol(TAG) accumulation in the liver along with reductions in liver function,immune dysfunction and a state of inflammation and oxidative stress. Mobilization of muscle and gluconeogenesis are also enhanced, while intake of vitamins and minerals is decreased, contributing to metabolic and immune dysfunction and oxidative stress. Enhancing post-ruminal supply of methyl donors is one approach that may improve immunometabolism and production synergistically in peripartal cows. At the cellular level, methyl donors(e.g. methionine, choline, betaine and folic acid) interact through one-carbon metabolism to modulate metabolism,immune responses and epigenetic events. By modulating those pathways, methyl donors may help increase the export of very low-density lipoproteins to reduce liver TAG and contribute to antioxidant synthesis to alleviate oxidative stress. Thus, altering one-carbon metabolism through methyl donor supplementation is a viable option to modulate immunometabolism during the peripartal period. This review explores available data on the regulation of one-carbon metabolism pathways in dairy cows in the context of enzyme regulation, cellular sensors and signaling mechanisms that might respond to increased dietary supply of specific methyl donors. Effects of methyl donors beyond the one-carbon metabolism pathways, including production performance, immune cell function,mechanistic target or rapamycin signaling, and fatty acid oxidation will also be highlighted. Furthermore, the effects of body condition and feeding system(total mixed ration vs. pasture) on one-carbon metabolism pathways are explored. Poten

关键词： Epigenetics Immune system Metabolism Methyl donor Transition period

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Green tea polyphenols supplementation alters immunometabolism and oxidative stress in dairy cows with hyperketonemia

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Animal Nutrition 2021年第1期7卷 206-215页

作者： Yanfen Ma Ying Feng Liwen Song Muyang Li Hongyu Dai Hua Bao Guijie Zhang Lei Zhao Chunhua Zhang Jing Yi yusheng liang School of Agriculture Ningxia UniversityYinchuan 750021China Institute of Animal Nutrition and Feed Inner Mongolia Academy of Agriculture and Animal Husbandry SciencesHohhot 010031China College of Food Engineering&Biotechnology Tianjin University of Science&TechnologyTianjin 300457China College of Animal Science and Veterinary Medicine Heilongjiang Bayi Agricultural UniversityDaqing 163316China College of Veterinary Medicine Nanjing Agricultural UniversityNanjing 210095China Department of Animal Sciences and Division of Nutritional Sciences University of IllinoisUrbana 61801USA

Peripartal cows often experience negative energy balance,and are therefore prone to suffering from metabolic diseases such as hyperketonemia,which causes financial losses in dairy *** study aimed to investigate the effect of green tea polyphenol(GTP)supplementation during the periparturient period on production performance,oxidative stress and immunometabolism in dairy cows with *** hundred Holstein cows were assigned to GTP(0.2 g/kg DM;n=50)or control(without GTP;n=50)group based on body weight,previous milk yield,and parity on d 15 before expected ***,10 cows with hyperketonemia were selected from each group,according to bloodβ-hydroxybutyric acid(BHBA)concentration between 1.2 and 2.9 mmol/L from 2 to 3 d *** cows were fed a close-up diet and a lactation diet with or without GTP supply from 15 d prepartum until 30 d *** and blood samples were obtained from 20 cows selected with hyperketonemia on 10,20,and 30 d *** with control cows,greater milk yield and lower somatic cell count were observed in GTP *** GTP group had lower concentrations of BHBA,free fatty acids,cholesterol,triglyceride,reactive oxygen species,malondialdehyde,and hydrogen peroxide,greater concentrations of glucose,lower activities of aspartate aminotransferase,alanine aminotransferase,and glutamyl transpeptidase,alongside greater ac-tivities of superoxide dismutase,glutathione peroxidase,and total antioxidant ***,GTP supplementation up-regulated concentrations of interleukin-6 and interleukin-10,but down-regulated concentrations of tumor necrosis factor-α,interleukin-1β,interleukin-2,interleukin-8,and interferon-γin *** concentrations of plasma immunoglobulin G were also detected in the GTP ***,the data suggested that GTP supplementation from 15 d prepartum to 30 d postpartum improved the milk yield and health status in cows with hvoerketonemia during early lactation.

关键词： Green tea polyphenol Hyperketonemia Peripartal period Dairy cow

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Evaluation of a povidone-iodine and chitosan-based barrier teat dip in the prevention of mastitis in dairy cows

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Journal of Integrative Agriculture 2021年第6期20卷 1615-1625页

作者： HANG Hui-min JIANG Hong-rui CHEN Dai-jie SHEN Zi-liang MAO Yong-jiang liang yusheng Juan JLOOR YANG Zhang-ping Key Laboratory for Animal Genetics BreedingReproduction and Molecular Design of Jiangsu ProvinceCollege of Animal Science and TechnologyYangzhou UniversityYangzhou 225009P.R.China Joint International Research Laboratory of Agriculture&Agri-Product Safety Ministry of Education/Yangzhou UniversityYangzhou 225009P.R.China Department of Animal Sciences&Division of Nutritional Sciences University of IllinoisUrbanaIL 61801USA

Postmilking teat dip is an important tool used to prevent mastitis in the modern dairy *** this study,we evaluated the in vitro and in vivo efficacies of a barrier teat dip containing povidone-iodine and chitosan for the prevention of *** experiment 1,we evaluated the antibacterial effects of chitosans with different molecular weights against six mastitis-causing bacteria based on the minimal inhibitory concentration *** results showed that 50 k Da chitosan had the maximum antibacterial activity compared with 5,150 and 350 k Da *** experiment 2,the inhibition zone test indicated that the barrier teat dip with 4.0%povidone-iodine and 1.0%chitosan had higher(P0.05)in SCC,somatic cell score(SCS)or other milk quality indicators

关键词： mastitis povidone-iodine chitosan barrier postmilking dip

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Transcription factor EB(TFEB)-mediated autophagy protects bovine mammary epithelial cells against H_(2)O_(2)-induced oxidative damage in vitro

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Journal of Animal Science and Biotechnology 2021年第3期12卷 1008-1018页

作者： Xudong Sun Renxu Chang Yan Tang Shengbin Luo Chunhui Jiang Hongdou Jia Qiushi Xu Zhihao Dong yusheng liang Juan J.Loor Chuang Xu Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases College of Animal Science and Veterinary MedicineHeilongjiang Bayi Agricultural UniversityNo.5 Xinyang RoadDaqing 163319Heilongjiang ProvinceChina College of Veterinary Medicine Hunan Agricultural UniversityChangsha 410128China Mammalian NutriPhysioGenomics Department of Animal Sciences and Division of Nutritional SciencesUniversity of IllinoisUrbana 61801USA

Background:Bovine mammary epithelial cells after calving undergo serious metabolic challenges and oxidative stress both of which could compromise *** factor EB(TFEB)-mediated autophagy is an important cytoprotective mechanism against oxidative ***,effects of TFEB-mediated autophagy on the oxidative stress of bovine mammary epithelial cells remain ***,the main aim of the study was to investigate the role of TFEB-mediated autophagy in bovine mammary epithelial cells experiencing oxidative ***:H_(2)O_(2) challenge of the bovine mammary epithelial cell MAC-T increased protein abundance of LC3-II,increased number of autophagosomes and autolysosomes while decreased protein abundance of *** of autophagy via bafilomycin A1 aggravated H_(2)O_(2)-induced reactive oxygen species(ROS)accumulation and apoptosis in MAC-T ***,H_(2)O_(2) treatment triggered the translocation of TFEB into the *** of TFEB by siRNA reversed the effect of H_(2)O_(2) on protein abundance of LC3-II and p62 as well as the number of autophagosomes and *** of TFEB activated autophagy and attenuated H_(2)O_(2)-induced ROS ***,TFEB overexpression attenuated H_(2)O_(2)-induced apoptosis by downregulating the caspase apoptotic ***:Our results indicate that activation of TFEB mediated autophagy alleviates H_(2)O_(2)-induced oxidative damage by reducing ROS accumulation and inhibiting caspase-dependent apoptosis.

关键词： Apoptosis Autophagy Bovine mammary epithelial cells Oxidative stress TFEB

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基于Hydrus-1D的黄河泥沙充填复垦土壤夹层结构优化

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农业工程学报 2022年第2期38卷 76-86页

作者：王晓彤胡振琪梁宇生北京大学城市与环境学院北京100080 中国矿业大学环境与测绘学院徐州221116 中国矿业大学(北京)土地复垦与生态重建研究所北京100083

中国东部矿区煤粮复合区面积大,因采煤沉陷积水,导致大面积的耕地破坏,采煤沉陷地复垦成为中国亟待解决的问题,黄河泥沙夹层式充填复垦技术对实现采煤沉陷地复垦具有积极意义。为优化黄河泥沙充填复垦夹层式土壤剖面设计,该研究采用Hydr... 详细信息

中国东部矿区煤粮复合区面积大,因采煤沉陷积水,导致大面积的耕地破坏,采煤沉陷地复垦成为中国亟待解决的问题,黄河泥沙夹层式充填复垦技术对实现采煤沉陷地复垦具有积极意义。为优化黄河泥沙充填复垦夹层式土壤剖面设计,该研究采用Hydrus-1D模拟分析了在充填复垦土壤总厚度一定的情况下,不同夹层厚度、位置和数量对重构土壤入渗和蒸发特性的影响。研究结果表明,黄河泥沙充填层中夹土壤层后能够有效改善重构土壤的水分运动特性并提高保水性,较“上土下沙”构型(CK2)入渗过程的持水性可提高12.79%,累积蒸发量可降低10.09%,持水系数可提高21.51%。从正交分析结果看,夹层厚度对持水系数影响最大,而夹层位置对持水系数影响最小,夹层位置和夹层数量的组合具有较强的交互效应。在土壤总厚度为60 cm的情况下,夹层厚度为30 cm,夹层位置为20 cm,夹层数量为2层时夹层式重构土壤的持水性最佳。研究探明了黄河泥沙夹层式充填复垦的夹层位置、厚度和数量对土壤水分运动的影响,并为黄河泥沙夹层式土壤剖面优化设计提出了一种经济时效的方法,对指导黄河泥沙夹层式充填复垦技术在黄河流域采煤塌陷地的应用推广具有积极作用。

关键词：入渗蒸发土壤采煤塌陷地矿区土地复垦 Hydrus-1D

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准噶尔盆地沙湾凹陷构造-地层层序与盆地演化

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石油与天然气地质 2018年第5期39卷 943-954页

作者：梁宇生何登发甄宇张磊田爱军中国地质大学(北京)能源学院北京100083 中国石油新疆油田分公司新疆克拉玛依834000

研究准噶尔盆地沙湾凹陷的构造-地层层序及盆地演化,不仅对于认识盆地结构及油气勘探具有重要意义,同时对中亚造山带构造演化的研究也具有一定的启示。文章利用井、震结合的思路,精细追踪地震同相轴,结合连井剖面对比,分析石炭纪以来各... 详细信息

研究准噶尔盆地沙湾凹陷的构造-地层层序及盆地演化,不仅对于认识盆地结构及油气勘探具有重要意义,同时对中亚造山带构造演化的研究也具有一定的启示。文章利用井、震结合的思路,精细追踪地震同相轴,结合连井剖面对比,分析石炭纪以来各地层内部的削蚀及超覆尖灭关系,并结合相应的构造体制转换过程,将研究区综合划分出6个大的构造层。依据断层相关褶皱理论开展精细构造解释,并利用平衡剖面技术,重点分析了沙湾凹陷自晚石炭世以来的构造演化。研究认为沙湾凹陷的形成演化经历了晚石炭世伸展断陷、早二叠世伸展拗陷、中二叠世—晚三叠世前陆盆地、侏罗纪陆内拗陷及压扭盆地、白垩纪—古近纪陆内拗陷及新近纪—第四纪陆内前陆盆地7个阶段,其演化过程主要受周缘边界断裂带多期活动的控制。西边界的红车断裂带在晚海西运动中期(P_2),印支运动晚期(T_3)及燕山运动中晚期(J_2-E)的强烈扭压作用对沙湾凹陷的构造演化及相应地层的发育与分布影响最强。

关键词：不整合面构造-地层层序盆地演化沙湾凹陷准噶尔盆地

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