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Unveiling phosphofructokinase as the key target: an innovative strategy combining pull-down assays with optical imaging for snail-killing mechanism exploration

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Science China Chemistry 2025年

作者： Yuxun Lu Weisi Wang Le Yu yuqiang zhao Ying Chen Yunhai Guo Bingrong Luo Yuhua Liu Shizhu Li Liping Duan Ying Zhou Yunnan Characteristic Plant Extraction Laboratory College of Chemical Science and Technology Yunnan University College of Biological and Chemical Engineering Guangxi University of Science and Technology National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research)NHC Key Laboratory of Parasite and Vector Biology WHO Collaborating Centre for Tropical Diseases National Center for International Research on Tropical Diseases School of Chemistry and Environment Yunnan Minzu University Dali Institute of Schistosomiasis Control

The Pomacea canaliculata, also known as apple snail, is an invasive species in China, posing a serious threat to agricultural production and public health. To prevent its rampant infestation, we developed a highly efficient snail-killing lead compound, 1-(4-chlorophenyl)-3-(pyridin-3-yl)urea (PBQ). However, the killing mechanism of PBQ remains unclear. To elucidate this, two novel compounds, B-PBQ and J-PBQ, were developed through structural modification of PBQ and were used, through both protein pull-down assay and confocal laser scanning microscopy imaging, to identify and confirm that phosphofructokinase(PFK) in the apple snail is the primary target protein of PBQ. Further studies revealed that PBQ successfully inhibits the activity,content, and expression of PFK in apple snail, disrupting the glycolysis pathway and leading to abnormal energy *** disruptions impair the snail’s tolerance to low oxygen, resulting in its efficient eradication. These findings offer a promising direction for developing next-generation PFK-targeted snail-killing pesticides. Furthermore, the strategy of combining pull-down assays with optical imaging used in this work provides a novel approach for the exploration of mechanisms of other pesticide candidates.

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Laser Quenching and Ion Sulphidizing Complex Surface Treat Technology for Diesel Engine Cylinder

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Journal of Wuhan University of Technology(Materials Science) 2012年第6期27卷 1115-1119页

作者：谢兆钱 ZENG Qingqiang HUANG Huayuan Cai Zhihai zhao yuqiang Northwest Institute of Nuclear Technology Xi'an 710024China National Defense Key Laboratory for Remanufacturing Technology Academy of Armored Forces EngineeringBeijing 100072China

In order to solve the problem of wear-out-failure of diesel engine cylinder, the laser-quenching and low temperature ion sulfurizing complex surface treatment technology was operated on the surface of 42MnCr52 steel. And the tribological properties of the complex layer were investigated. The experimental results indicated that the complex layer was composed of soft surface sulphide layer and sub-surface laser- quenching harden layer, and showed excellent friction-reduction and wear-resistance performance at high temperature. The synergistic effect of the complex layer resulted in 20% increase in hardness, 10% reduction in friction coefficient and 50% reduction in wear weight loss, respectively, compared with those of the standard samples. The bench-test further demonstrated that this technology can improve the lubricating condition between cylinder and piston ring, and reduce both abnormity wear when the lubricating oil is deficiency at the time of start-up and sticking wear at high temperature during the operating period, and then prolong the service life of engine.

关键词： cylinder laser quenching ion sulphidizing friction and wear

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云南5种蜂蜜12种糖组分和20种氨基酸含量的测定与分析

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食品科学 2025年第2期46卷 148-155页

作者：孙晓杰黄学者赵玉强贾光群孙艺涵徐向东崔宗岩河北医科大学公共卫生学院河北省环境与人群健康重点实验室河北石家庄050017 秦皇岛海关技术中心河北秦皇岛066004

为探究云南5种特色蜂蜜澳洲坚果蜜(Macadamia integrifolia honey)、咖啡蜜(Coffea honey)、米团花蜜(Leucosceptrum canum Smith honey)、苕子蜜(Vicia villosa Roth honey)及橡胶蜜(Hevea brasiliensis honey)糖组分和氨基酸含量水平和分布规律,对云南5种蜂蜜中12种糖组分及20种氨基酸含量进行测定、分析,并利用层次聚类分析和偏最小二乘判别分析(partial least squares-discriminant analysis,PLS-DA)探讨糖组分及氨基酸含量在5种蜂蜜分类鉴别中的应用。结果表明:云南5种蜂蜜中果糖、葡萄糖和蔗糖的含量符合行业标准要求。10种低聚糖总质量分数范围在5.35%~7.92%之间,其中松二糖与麦芽酮糖、曲二糖、异麦芽糖存在较强的相关性。5种特色蜂蜜的20种氨基酸含量之和范围在312.54~1245.51 mg/kg之间,其氨基酸总量关系为:咖啡蜜>米团花蜜>苕子蜜>橡胶蜜>澳洲坚果蜜。不同蜜种蜂蜜的氨基酸组成及含量呈现较为显著的差异,咖啡蜜中酪氨酸含量是其他蜜种蜂蜜的10倍以上,推测其为咖啡蜜潜在的特征标志物。聚类分析结果显示,根据氨基酸可以较好地将云南5种蜂蜜进行归类,准确率达98.5%。通过PLS-DA同样可以将5种蜂蜜进行有效区分,并筛选出脯氨酸、精氨酸、色氨酸、组氨酸、天冬酰胺、丙氨酸共6个变量投影重要性值>1的差异参数用于区分云南5种蜂蜜。

关键词：蜂蜜糖类氨基酸化学计量学

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A ratiometric near-infrared naphthalimide-based fluorescent probe with high sensitivity for detecting Fe^(2+)in vivo

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Chinese Chemical Letters 2020年第11期31卷 2941-2944页

作者： Lina Huang Yu Chen yuqiang zhao Yumin Wang Junwei Xiong Junfeng Zhang Xianghua Wu Ying Zhou College of Chemistry and Chemical Engineering Yunnan Normal UniversityKunming 650500China College of Chemical Science and Technology Yunnan UniversityKunming 650091China

Iron is one of the essential trace elements in the human *** plays an important role in human biology and *** of iron levels in cells is associated with disease *** this work,we synthesized a novel near-infrared intramolecular charge transfer(ICT)based ratiometric fluorescent probe to detect Fe^(2+),by using naphthalimide and indole moieties as building *** work showed that the radiometric probe has excellent selectivity,sensitivity and rapid ***,we could successfully perform real-time monitoring of Fe^(2+) in He La cells and ***.

关键词： Fluorescent probe Ratiometric Fe^(2+) Naphthalimide Near-infrared

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Adjustable thermal expansion in La(Fe,Si)13-based conductive composites by high-pressure synthesis

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Progress in Natural Science:Materials International 2019年第1期29卷 28-31页

作者： Wei Wang Rongjin Huang Yi Shan yuqiang zhao Xinran Shan Shibin Guo Chuanjun Huang Laifeng Li School of Materials Science and Engineering Tianjin University State Key Laboratory of Technologies in Space Cryogenic Propellants Technical Institute of Physics and Chemistry Chinese Academy of Sciences University of Chinese Academy of Sciences

Providing a suitable contact interface, where a high conductivity material with a desirable coefficient of thermal expansion(CTE) adjoins the target micro-electric devices, is very crucial to optimize the properties and service life of the relevant instruments. Regrettably, a high conductivity, low thermal expansion and relatively inexpensive material is very rare. Composites, fortunately, can offer a method to design materials with adjustable properties by mixing two or more diverse constituents. In this paper, high conductivity composites with adjustable thermal expansion were successfully prepared by a high-pressure synthesis. The composites are based on combining La(Fe,Si)-based compounds, the materials showing a giant, isotropic negative thermal expansion(NTE) properties, within Cu matrix. The La(Fe,Si)-based compounds were used to adjust the CTE of the composites, while the Cu phase is in charge of tuning the thermal conductivity properties. Thus, by changing the relative amount of the two components, the composites with high conductivities and adjustable CTE were achieved. Furthermore, the thermal expansion and magnetic properties of the composites were investigated by a physical property measurement system. The present results highlight the potential applications of the Cu-based high conductivity composites with room-temperature NTE properties in the thermal contacts to various semiconductor and microelectronic devices.

关键词： La(Fe, Si)13-based compounds Metal-matrix composites Negative thermal expansion

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Effects of the molluscicide can didate PPU06 on alkaline phosphatase in the golden apple snails determined using a near-infrared fluorescent probe

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Chinese Chemical Letters 2021年第5期32卷 1809-1813页

作者： Changxiaoxi Liu Suo Yang Yimu Qiao yuqiang zhao Weisi Wang Mingxuan Jia Yanqi He Ying Zhou Liping Duan College of Chemical Science and Technology Yunnan UniversityKunming 650091China National Institute of Parasitic Diseases Chinese Center for Disease Control and PreventionWHO Collaborating Centre for Malaria.Schistosomiasisand FilariasisKey Laboratory of Parasitology and Vector Biology of the Chinese Ministry of HealthShanghai 200025China Qinghai Provincial People's Hospital Xining 810007China

We constructed a reaction-based near-infrared fluorescent probe(Niap) to specifically identify alkaline phosphatase(ALP) with fast red fluorescence *** on the positive concentrationdependent manner between the fluorescent intensity of the Niap and ALP,probe Niap was used to study the ALP enrichment and variation in golden apple snails(Pomacea canaliculata) exposed to the molluscicide candidate *** treatment with different concentrations of PPU06 over various times,three organs of the surviving snails,liver,stomach and plantaris,were frozen and sectioned for fluorescent imaging *** increased PPU06 concentration,red fluorescence substantially increased in the liver and reached a maximum within 24 h when the PPU06 co ncentration was 0.75 mg/*** obvious changes in the stomach or foot plantaris were *** showed PPU06 caused liver injury and stimulated the increase of ALP in the liver of *** study demonstrates a rapid ALP fluorescent identification method that can be used to study the effects of PPU06 on *** also provides optical evidence that may aid in the discovery of new chemistry for snail control.

关键词： Fluorescence sensor ALP Golden apple snails(R canaliculata) Molluscicide candidate Bio-imaging

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Carbon and nitrogen mineralization in soils along a desertification gradient in the semiarid Horqin Sandy Land, Northern China

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Research in Cold and Arid Regions 2010年第1期2卷 21-30页

作者： yuqiang Li HaLin zhao TongHui Zhang YuLin Li XiaoAn Zuo Cold and Arid Regions Environmental Engineering Research Institute Chinese Academy of Sciences Lanzhou Gansu 730000 China

This experiment was conducted in three sites along a desertification gradient in Horqin Sandy Land, Northern China. Soils una-mended and amended with five types of plant residue in a wide range of C：N ratios from 9.9 to 82.2 were incubated for 70 days, during which C and N mineralization were measured. Along the desertification gradient from fixed sand dune to semifixed, and mobile sand dune： cumulative CO2-C produced from the unamended soils was 231.6, 193.3 and 61.9 μg/g, respectively, while net inorganic N was 22.9, 17.6 and 0.9 mg/kg. Soils amended with residues produced more CO2-C than the unamended soils across all sites. During the first 10 days, C mineralization rate of residue-amended soils decreased with the increase of C：N ratio at each site. However, the mineralization rates were poorly correlated with the C：N ratio in subsequent stage of incubation. Soils of mobile sand dune amended with higher C：N ratio （more than 32） residues produced less CO2-C than that of fixed and semifixed sand dune. NO3--N was the predominant form of inorganic N during the mineralization process in sandy soils. Carbon-to-nitrogen ratio （C：N） can be regarded as a predictor of the speed of N mineralization in sandy soil. The more C. microphylla residue with the lowest C：N ratio （9.9） added in soils, the more net inorganic N released. Our results suggest that C. microphylla residue when added to soil would potentially provide short-term plant available N and improve the soil quality in sandy land. The desertification process postponed the release of inorganic N from plant residues.

关键词： carbon and nitrogen mineralization residue amendment desertification Horqin Sandy Land

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Robust Global Asymptotic Stability of Uncertain Nonholonomic Systems with Strong Nonlinear Drifts

Robust Global Asymptotic Stability of Uncertain Nonholonomic...

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第二十九届中国控制会议

作者： WU yuqiang,zhao Yan Institute of Automation,Qufu Normal University,Qufu 273165,P.R.China

The robust stability is investigated for nonholonomic control systems in generalized chained form with strong nonlinear *** order to make the origin be a solution of the closed-loop system and prevent the finite time escape phenomenon from happening,anew robust nonlinear switching law is proposed sothat global asymptotic stability analysis can be dealt with for discontinuous closed-loop *** recursive integrator backstepping technique is applied to the design of the robust *** simulation example demonstrates the effectiveness and robust features of the proposed method.

关键词： Nonholonomic systems with uncertainties Robust nonlinear control Integrator backstepping Switching control strategy Global asymptotic stability

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不同进风方案下隧道烘干窑热风流场CFD模拟和优化

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农业工程学报 2014年第14期30卷 315-321页

作者：师建芳吴中华刘清娄正赵玉强朱明农业部规划设计研究院农产品加工工程研究所北京100125 天津科技大学机械工程学院天津300222

隧道烘干窑内同一横截面的热风均匀性影响着物料干燥均匀性和产品质量,而烘干窑入口风速分布直接影响着窑内热风流场的均匀分布。为了解决单一风机直进风隧道烘干窑存在的风速不均匀问题,提出了多种进风结构设想,并利用计算流体力学方... 详细信息

隧道烘干窑内同一横截面的热风均匀性影响着物料干燥均匀性和产品质量,而烘干窑入口风速分布直接影响着窑内热风流场的均匀分布。为了解决单一风机直进风隧道烘干窑存在的风速不均匀问题,提出了多种进风结构设想,并利用计算流体力学方法对实际生产的隧道窑进风流场进行数值模拟,研究3种不同的进风方案(4风机、6风机和9风机)对隧道窑内热风流场均匀性的影响。模拟结果表明:6风机方案下隧道窑入口处进风均匀,热风扩散距离短,窑内热风流场整体均匀性较佳,综合性价比最高。研究结果为隧道窑入口进风的设计提供参考。

关键词：计算流体力学数值分析干燥流场热风干燥隧道式烘干窑

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“闲”始源字形与含义补阙——从《汉书》“闲驹”之“闲”不当训“阑”说起

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湖北理工学院学报（人文社会科学版） 2020年第3期37卷 53-58页

作者：赵玉强杭州师范大学哲学系浙江杭州311121

“闲”是中国文化中的重要词汇。除了“门中有木”的渊源外,“闲”当还有一个待补阙的字源“姦馬”。南朝《玉篇》以“姦馬”为“闲”古字,元代《六书统》等释其为“马步习”之义,先秦及后世众多与马、车骑、旗帜、钟鼓、军旅等相关的... 详细信息

“闲”是中国文化中的重要词汇。除了“门中有木”的渊源外,“闲”当还有一个待补阙的字源“姦馬”。南朝《玉篇》以“姦馬”为“闲”古字,元代《六书统》等释其为“马步习”之义,先秦及后世众多与马、车骑、旗帜、钟鼓、军旅等相关的“闲”“闲闲”“简简”“阑阑”等词,均可与“姦馬”义发明互证。惜乎明清以降,诸字书再不见“闲”与“姦馬”的关联。“姦馬”的发现可为文艺学、美学、休闲学等相关“休闲”研究辟出新义。

关键词：闲姦馬简简阑阑休闲

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