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Allosteric DNAzyme-based encoder for molecular information transfer

Chinese Chemical Letters 2024年第7期35卷 235-241页

作者： Minghao hu Tianci Xie yuqiang hu Longjie Li Ting Wang Tongbo Wu School of Pharmacy Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Oncology Tongji Hospital of Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China GI Cancer Research Institute Tongji HospitalHuazhong University of Science and TechnologyWuhan 430030China School of Life Science and Technology Wuhan Polytechnic UniversityWuhan 430023China State Key Laboratory of Bioelectronics National Demonstration Center for Experimental Biomedical Engineering EducationSchool of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China

Dynamic DNA nanotechnology plays a significant role in nanomedicine and information science due to its high programmability based on Watson-Crick base pairing and nanoscale *** DNA machines and networks have been widely used in various fields,including molecular imaging,biosensors,drug delivery,information processing,and logic *** serve as crucial components for information compilation and transfer,allowing the conversion of information from diverse application scenarios into a format recognized and applied by DNA ***,there are only a few encoder designs with DNA ***,the molecular priority encoder is hardly *** this study,we introduce allosteric DNAzyme-based encoders for information *** design of the allosteric domain and the recognition arm allows the input and output to be independent of each other and freely *** pre-packaged mode design achieves uniformity of baseline dynamics and dynamics *** also integrated non-nucleic acid molecules into the encoder through the aptamer design of the allosteric ***,we developed the 2^(n)-n encoder and the EndoⅣ-assisted priority encoder inspired by immunoglobulin's molecular structure and effector *** our knowledge,the proposed encoder is the first enzyme-free DNA encoder with DNA output,and the priority encoder is the first molecular priority encoder in the DNA reaction *** encoders avoid complex operations on a single molecule,and their simple structure facilitates their application in complex DNA circuits and biological scenarios.

关键词： DNAzyme Encoder Nucleic acids DNA circuit DNA strand displacement Dynamic DNA nanotechnology

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Addressable DNA Information Processing System with a Fluorescent Readout for Rewritable Memory

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Chinese Journal of Chemistry 2023年第20期41卷 2628-2634页

作者： Zirui Xi Mengyao Yang yuqiang hu Tongbo Wu School of Pharmacy Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei430030 China

DNA-based nanostructure allows the construction of molecular devices useful in biological computing and information ***,an addressable and editable DNA information processing system established on a fluorescence intensity signal detection platform to save and encrypt information is *** system operates by encoding information into distinct and changeable units of the trigger strands decoding by fluorescence intensity signal *** toehold-mediated strand displacement reactions,the trigger strand can be precisely added to and removed from the memorizer and reporter to implement the function of editing,encrypting,and *** strategy is simple to implement,requiring only two mixing steps at room temperature for each operation and fluorescence intensity signal detection to read the *** the system can realize accurate retrieval of specific individual information,eliminating all unnecessary redundant *** of its point-to-point accurate readout and programmability,the system is expected to become a powerful tool for the future development of information storage and sensing of biological molecules.

关键词： Information processing Strand displacement Self-assembly Fluorescence DNA structures

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DNA Reaction Network Mimicking the Basic Steps of Synaptic Communication

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Chinese Journal of Chemistry 2023年第11期41卷 1313-1318页

作者： Minghao hu yuqiang hu Wenqian Yuan Zhen Zhang Tongbo Wu School of Pharmacy Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei430030 China

Biological systems use intricate networks of chemical reactions to exchange information. How to simulate complex systems with simple strand-displacement reactions is crucial to broaden the application scenario of the DNA reaction network. Here, we report the artificial DNA reaction network to mimic the operation and function of biological information transfer via strand-displacement reaction. DNA is used as simple artificial analogs to schematize structures and transmit information. Using chemical synapses in neural networks as an example, we show that the proposed network enables core functions of biological systems, such as the long-term potential of synapses, which underpin learning and memory. Also, we performed the simple “silicon mimetic” to link electronic circuits to chemical network-based biological structures. As such, synaptic communication simulated by the DNA reaction network provides a complete demonstration for designing artificial reaction networks based on the essence of information interaction.

关键词： Reaction networks Strand-displacement cascades DNA nanotechnology Synapse Nucleic acids Fluorescence Synthetic biology

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Probing and modulating the interactions of the DNAzyme with DNA-functionalized nanoparticles

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Chinese Chemical Letters 2022年第6期33卷 3026-3030页

作者： yuqiang hu Zhen Zhang Wei Zhang Minghao hu Xianjin Xiao Tongbo Wu School of Pharmacy Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Institute of Reproductive Health/Center of Reproductive Medicine Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Obstetrics and Gynaecology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

DNA-functionalized gold nanoparticles are one of the most versatile bionanomaterials for biomedical and clinical diagnosis. Herein, we discovered that the performance of DNAzyme cleaving the substrate is highly related to its length. This intriguing phenomenon only appears at the interfaces of DNAfunctionalized gold nanoparticles. We systematically investigated the causes of this phenomenon. We conjectured that the DNAzyme with extended nucleotides that do not match its substrate strand is vulnerable to non-specific adsorption, electrostatic repulsion, and steric hindrance. Based on our improved understanding of this phenomenon, we have successfully developed a highly sensitive and specific amplifiable biosensor to detect human apurinic/apyrimidinic endonuclease 1.

关键词： Gold nanoparticles DNAzyme Interface interaction Rate difference human apurinic/apyrimidinic endonuclease 1

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Sensitive DNA Mutation Detection at Physiological Temperature with Endonuclease IV by Inhibiting Its Side Activity

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Chinese Journal of Chemistry 2021年第9期39卷 2477-2482页

作者： Zhen Zhang Longjie Li Qingxu Liu yuqiang hu Wenqian Yuan Xianjin Xiao Tongbo Wu School of Pharmacy Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430030China School of Molecular Medicine Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhouZhejiang 310024China Department of Oncology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430030China Institute of Reproductive Health/Center of Reproductive Medicine Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430030China

Endonuclease IV(Endo IV)has the side activity to cleave the apurinic/apyrimidinic site in the single-stranded DNA probe(AP-ssDNA),which adversely affects its performance in single-nucleotide variation(SNV)*** this work,we developed a simple strategy to inhibit Endo IV's side activity by introducing an assistant strand into the detection *** assistant strand effectively inhibited the side-activity of Endo IV by conf ini ng the freedom of AP-ssDNA through rigid double-stranded DNA formati on,and the inhibition efficiency could reach 98%.About 20 times enhancement of the discrimination factor(DF)was obtained compared to the detection system without the assistant *** optimization,a mean DF value was calculated to be 738 for different mutation types,and the sample with 0.005%allele frequency was discriminated from the wild-type *** advantage of using an assists nt stra nd was that the SNV detection could be executed at physiological temperature without precise temperature optimization as in other Endo IV-based SNV detection ***,EGFR T790M mutant in the synthesized and clinical samples was detected by our *** results showed satisfactory sensitivity and accuracy by comparison with Sanger ***,this strategy has the potential to be applied in the field of precision medicine.

关键词： DNA mutation Enzymes Side activity Fluorescent probes Biose nsors

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无语言的孤独症和智力残疾儿童自发手势的发展研究

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中国特殊教育 2005年第5期 73-77页

作者：王梅胡玉强北京联合大学特殊教育学院北京100075 北京通州培智中心学校北京101100

自发手势及其分类研究有助于公众更好地理解其意,从而增加相互交流的可能性。本文对20名无语言的孤独症和智力残疾儿童在语言沟通有障碍时利用自发手势进行沟通的情况进行了观察研究,研究结果表明两者在发生频率上没有区别,但在表达内... 详细信息

自发手势及其分类研究有助于公众更好地理解其意,从而增加相互交流的可能性。本文对20名无语言的孤独症和智力残疾儿童在语言沟通有障碍时利用自发手势进行沟通的情况进行了观察研究,研究结果表明两者在发生频率上没有区别,但在表达内容、目的性、动作姿势等方面存在一定差异。

关键词：孤独症智力残疾自发手势儿童特殊教育

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316L表面激光熔覆NiTi合金涂层的组织结构及元素占位

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复合材料学报 2024年第2期41卷 1030-1037页

作者：丰玉强胡正飞同济大学材料科学与工程学院上海201804 上海市金属功能材料开发应用重点实验室上海201804

60NiTi(60wt%Ni-40wt%Ti)合金具有高硬度、高弹性模量及优异的耐磨和耐腐蚀性能,在许多领域有潜在的应用价值。本文对激光熔覆方法在316L不锈钢表面制备NiTi涂层的微观组织结构和物相组成进行了分析。结果表明:基材中主要成分Fe和Cr扩... 详细信息

60NiTi(60wt%Ni-40wt%Ti)合金具有高硬度、高弹性模量及优异的耐磨和耐腐蚀性能,在许多领域有潜在的应用价值。本文对激光熔覆方法在316L不锈钢表面制备NiTi涂层的微观组织结构和物相组成进行了分析。结果表明:基材中主要成分Fe和Cr扩散到熔覆涂层中并显著地影响了镍钛涂层的组织结构和性能。熔覆涂层主要组成相为NiTi和Ni_(3)Ti,提高Ni含量可抑制NiTi_(2)析出。第一性原理计算结果显示,Fe和Cr元素易于固溶在B2结构的NiTi相中,并且都倾向于置换晶胞中的Ni原子。Ni_(3)Ti晶胞结构中的Ni和Ti原子各有2个不同的晶位,Fe原子倾向于置换晶胞中Ni-1晶位的Ni原子,而Cr原子倾向于置换晶胞中Ni-2晶位的Ni原子。根据涂层的成分分析结合模拟可知,镍钛合金涂层中NiTi和Ni_(3)Ti相的化学式分别为Ni_(5)Ti_(8)Fe_(2)Cr和Ni_(9)Ti_(4)Fe_(2)Cr。

关键词：激光熔覆镍钛合金第一性原理合金元素掺杂择优占位表面改性

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加强造价管理满足水利工程建设需要

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中国水利 2010年第20期 7-9,16页

作者：王治明胡玉强水利部水利水电规划设计总院北京100120

水利工程造价管理在水利工程建设投资的确定与管理方面发挥着愈加重要的作用。结合当前的水利工程建设实际,合理确定工程投资,改进造价控制方法,提高造价管理水平,改进概算编制方法,严格控制工程投资,保证工程环境的生态投资需求,不断... 详细信息

水利工程造价管理在水利工程建设投资的确定与管理方面发挥着愈加重要的作用。结合当前的水利工程建设实际,合理确定工程投资,改进造价控制方法,提高造价管理水平,改进概算编制方法,严格控制工程投资,保证工程环境的生态投资需求,不断推进水利工程造价管理体系建设。但现行的水利工程造价标准存在一些不足之处,需吸收国外造价管理经验,不断满足水利工程建设管理新要求,开拓水利工程造价管理新局面。

关键词：水利工程造价管理体系

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Design of Multi-Ferrocenyl Alkyne Cobalt Carbonyl Cluster

Design of Multi-Ferrocenyl Alkyne Cobalt Carbonyl Cluster

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中国化学会第五届全国结构化学学术会议

作者： Limin Han Quanling Suo Yanqiang Ma yuqiang hu (Chemical Engineering College,Inner Mongolia University of Technology,Hohhot,010051)

＜正＞Design of molecular electronic device,such as molecular wire,switch,sensor,motor and so on, often need to introduce the metal atom or metal cluster framework into a conjugated *** alkyne cobalt complexes are bet...

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计算机数据库技术在信息管理中的应用

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软件 2022年第12期43卷 112-114页

作者：胡钰强四川外国语大学成都学院四川成都611844

随着社会经济的快速发展,以及科学技术的不断进步,计算机技术、互联网技术等在各行业领域和人们日常生活中得到广泛应用,其中通过计算机网络信息技术,能够实现数据快速收集、整理、分析和管理,有效促进经济、文化发展的同时,也为人们生... 详细信息

随着社会经济的快速发展,以及科学技术的不断进步,计算机技术、互联网技术等在各行业领域和人们日常生活中得到广泛应用,其中通过计算机网络信息技术,能够实现数据快速收集、整理、分析和管理,有效促进经济、文化发展的同时,也为人们生活、学习带来极大便利。另外在信息管理中,通过计算机数据库的应用,能够提高数据管理质量和效率。本文结合计算机数据库技术特点、结构和应用特征等,对其在信息管理中的应用现状进行分析,并对数据库技术应用改进策略进行探讨。

关键词：计算机数据库技术数据结构信息管理

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