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Discovery of Cladonema multiramosum ***.(Cnidaria:Hydrozoa:Cladonematidae)using DNA barcoding and life cycle analyses

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Acta Oceanologica Sinica 2022年第3期41卷 44-52页

作者： Konglin Zhou yuping gu Lu Wang Jianming Chen Institute of Oceanography College of Geography and OceanographyMinjiang UniversityFuzhou 350108China

In contrast to typical planktonic hydromedusae,Cladonema medusae are mostly benthic,with specialised adhesive branches to adhere to the *** this study,a Cladonema species discovered in a laboratory aquarium in Fuzhou,China,was confirmed as a new species,based on morphological and molecular *** species was named Cladonema multiramosum *** medusa is distinct from that of congeners possessing substantially more adhesive branches(8-24,rarely 5-7),and tiny branches on the upper radial *** validity of *** *** also supported by molecular phylogenetic analyses based on the mitochondrial 16S rDNA ***,*** *** also displayed considerable phenotypic plasticity with respect to its radial canals,tentacles,stinging branches per tentacle,oral tentacles,manubrium,and gonads,hindering species identification based solely on *** some morphological characteristics of hydroids(filiform tentacles and medusa buds)and nematocysts could also be used as diagnostic characters in the genus Cladonema,this information is unavailable for some Cladonema ***,the taxonomy within the genus Cladonema was re-evaluated based mainly on the morphological characteristics of the *** revision of the genus Cladonema should be made when supplementary information on the life cycle and DNA barcoding are updated.

关键词： Cladonema morphology life cycle DNA barcoding 16S rDNA

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基于表面肌电信号的上肢康复动作识别

洛阳理工学院学报（自然科学版） 2024年第3期34卷 56-61页

作者：顾玉平李宪华张康罗耀安徽理工大学人工智能学院安徽淮南232001 安徽理工大学机电工程学院安徽淮南232001

选取上肢肱桡肌、尺侧弯曲肌、肱肌、肱二头肌、三角肌作为采集对象,使用干电极片以及数据采集卡采集表面肌电信号(sEMG)。将采集到的表面肌电信号进行预处理,提取时域特征、频域特征以及信息熵特征,将提取的特征用于卷积神经网络模型... 详细信息

选取上肢肱桡肌、尺侧弯曲肌、肱肌、肱二头肌、三角肌作为采集对象,使用干电极片以及数据采集卡采集表面肌电信号(sEMG)。将采集到的表面肌电信号进行预处理,提取时域特征、频域特征以及信息熵特征,将提取的特征用于卷积神经网络模型的训练,再抽取特征值部分样本作为验证集合进行交叉验证。实验结果表明,融合信息熵作为特征样本训练准确率高达91%,明显高于单一特征样本以及融合时频域特征样本。

关键词：表面肌电信号模式识别康复运动卷积神经网络

端粒酶、γ-干扰素、腺苷酸脱氨酶及结核感染T细胞斑点试验在诊断结核性和恶性胸腔积液中的作用分析

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海军医学杂志 2024年第1期45卷 53-57页

作者：由振华黄锦宏赵云根顾宇平程易凌春华常熟市第二人民医院呼吸内科江苏常熟215500 苏州大学附属第一人民医院呼吸内科

目的评价端粒酶、γ-干扰素(IFN-γ)、腺苷酸脱氨酶(ADA)及T细胞斑点试验(***)检测在诊断结核性和恶性胸腔积液中的价值。方法纳入40例早期结核性胸膜炎患者(结核组)和40例恶性胸腔积液患者(肿瘤组),分别在治疗前抽取患者胸腔积液及... 详细信息

目的评价端粒酶、γ-干扰素(IFN-γ)、腺苷酸脱氨酶(ADA)及T细胞斑点试验(***)检测在诊断结核性和恶性胸腔积液中的价值。方法纳入40例早期结核性胸膜炎患者(结核组)和40例恶性胸腔积液患者(肿瘤组),分别在治疗前抽取患者胸腔积液及空腹血液,应用酶联免疫吸附分析法检测端粒酶的含量和IFN-γ的浓度,应用比色分析法检测ADA的活性,采用Elispot斑点计数检测***阳性率。结果结核组和肿瘤组的端粒酶含量分别为(1.46±0.73)×10~(-3) nmol/L和(3.34±1.72)×10~(-3) nmol/L,结核组端粒酶含量低于肿瘤组(P<0.01);结核组和肿瘤组的IFN-γ含量分别为(137.44±38.93) U/L和(27.94±8.46) U/L,ADA含量分别为(68.42±9.58) U/L和(15.39±4.43) U/L,2组患者IFN-γ含量和ADA含量比较,差异有统计学意义(P<0.01)。结核组IFN-γ浓度、ADA活性及***阳性率均高于肿瘤组(P<0.01)。联合应用端粒酶、IFN-γ、ADA及*** 4个诊断指标的诊断试验鉴别诊断结核性和恶性胸腔积液的敏感度为0.974,特异度为0.974。结论联合应用端粒酶、IFN-γ、ADA及*** 4个诊断指标的诊断试验在结核性胸腔积液和恶性胸腔积液的鉴别诊断中具有重要意义。

关键词：端粒酶 γ-干扰素腺苷酸脱氨酶 T细胞斑点试验结核性胸腔积液恶性胸腔积液

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大学精神的起点——“真”的哲学视域

现代大学教育 2007年第6期23卷 4-8页

作者：顾玉平南京大学

大学精神的起点是真,真沿着自由、真知、引领展开,真知之境、自由之境与至善之境升华为至真至圣之境,大学遐想之美,逻辑之美,崇高之美由此降生,变幻出大学万千世界来。大学万千世界又复归于真,如此循环往复,以至无穷。

关键词：大学精神言说与不可言说大学之真

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拟除虫菊酯在纸质文献防蛀上的再探索

图书馆杂志 2022年第8期41卷 115-120页

作者：顾彧平上海图书馆(上海科学技术情报研究所)典藏中心上海200031

防虫防蛀是纸质文献、档案保存中的难题与重点,应用拟除虫菊酯药液染制防蠹纸,以起到对常见蛀虫的触杀和缓释驱避作用。文章在相关应用研究的基础上对拟除虫菊酯药液配方进行改进,在进一步增强防虫效果的同时确保拟除虫菊酯新药液对人... 详细信息

防虫防蛀是纸质文献、档案保存中的难题与重点,应用拟除虫菊酯药液染制防蠹纸,以起到对常见蛀虫的触杀和缓释驱避作用。文章在相关应用研究的基础上对拟除虫菊酯药液配方进行改进,在进一步增强防虫效果的同时确保拟除虫菊酯新药液对人体的安全性。通过在上海图书馆普通古籍书库近三年的使用实践,初步验证了新药液染制的防蠹纸用于古籍虫害防治的可行性与有效性,为图书馆、档案馆等纸质文献、档案收藏保藏机构提供了一种防虫防蛀的解决思路与方案。

关键词：拟除虫菊酯防蛀纸质文献档案

The gene encoding flavonol synthase contributes to lesion mimic in wheat

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The Crop Journal 2024年第3期12卷 814-825页

作者： Tingting Dong Hongchun Xiong Huijun guo Yongdun Xie Linshu Zhao Jiayu gu Huiyuan Li Shirong Zhao yuping Ding Xiyun Song Luxiang Liu College of Life Sciences Key Laboratory of Major Crop Germplasm Innovation and Application in QingdaoQingdao Agricultural UniversityQingdao 266109ShandongChina State Key Laboratory of Crop Gene Resources and Breeding/National Engineering Laboratory of Crop Molecular Breeding/CAEA Research and Development Centre on Nuclear Technology Applications for Irradiation Mutation Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

Lesion mimic often exhibits leaf disease-like symptoms even in the absence of pathogen infection,and is characterized by a hypersensitive-response(HR)that closely linked to plant disease *** this,only a few lesion mimic genes have been identified in *** this investigation,a lesion mimic wheat mutant named je0297 was discovered,showing no alteration in yield components when compared to the wild type(WT).Segregation ratio analysis of the F_(2)individuals resulting from the cross between the WT and the mutant revealed that the lesion mimic was governed by a single recessive gene in *** Bulked segregant analysis(BSA)and exome capture sequencing,we mapped the lesion mimic gene designated as lm6 to chromosome *** gene fine mapping using 3315 F_(2)individuals delimited the lm6 within a 1.18 Mb *** this region,we identified 16 high-confidence genes,with only two displaying mutations in ***,one of the two genes,responsible for encoding flavonol synthase,exhibited altered expression *** phenotype analysis of TILLING mutants confirmed that the gene encoding flavonol synthase was indeed the causal gene for *** sequencing analysis revealed that the DEGs between the WT and mutant were significantly enriched in KEGG pathways related to flavonoid biosynthesis,including flavone and flavonol biosynthesis,isoflavonoid biosynthesis,and flavonoid biosynthesis ***,more than 30 pathogen infection-related(PR)genes exhibited upregulation in the *** to this expression pattern,the flavonoid content in je0297 showed a significant decrease in the 4^(th)leaf,accompanied by a notable accumulation of reactive oxygen,which likely contributed to the development of lesion mimic in the *** investigation enhances our comprehension of cell death signaling pathways and provides a valuable gene resource for the breeding of disease-resistant wheat.

关键词： Lesion mimic mutant Wheat Gene mapping Flavonol synthase gene Flavonoid

Chromosomal-level genome and metabolome analyses of highly heterozygous allohexaploid Dendrocalamus brandisii elucidate shoot quality and developmental characteristics

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Journal of Integrative Plant Biology 2024年第6期66卷 1087-1105页

作者： Jutang Jiang Zeyu Zhang Yucong Bai Xiaojing Wang yuping Dou Ruiman Geng Chongyang Wu Hangxiao Zhang Cunfu Lu Lianfeng gu Jian Gao Key Laboratory of National Forestry and Grassland Administration Beijing for Bamboo&Rattan Science and TechnologyInstitute of Gene Science and Industrialization for Bamboo and Rattan ResourcesInternational Center for Bamboo and RattanBeijing 100102China College of Forestry Basic Forestry and Proteomics Research CenterFujian Agriculture and Forestry UniversityFuzhou 350002China School of Life Science Peking UniversityBeijing 100871China College of Biological Sciences and Biotechnology Beijing Forestry UniversityBeijing 100083China

Dendrocalamus brandisii(Munro)Kurz is a sympodial bamboo species with inimitable taste and flavorful *** rapid growth and use as high-quality material make this bamboo species highly valued for both food processing and wood ***,genome information for *** is lacking,primarily due to its polyploidy and large genome ***,we assembled a high-quality genome for hexaploid ***,which comprises 70chromosomes with a total size of 2,756 Mb,using long-read Hi Fi ***,we accurately separated the genome into its three constituent *** used Oxford Nanopore Technologies long reads to construct a transcriptomic dataset covering 15 tissues for gene annotation to complement our genome assembly,revealing differential gene expression and post-transcriptional *** integrating metabolome analysis,we unveiled that well-balanced lignin formation,as well as abundant flavonoid and fructose contents,contribute to the superior quality of *** *** genomic,transcriptomic,and metabolomic datasets provided a solid foundation for enhancing bamboo shoot quality and developing efficient gene-editing *** study should facilitate research on *** and enhance its use as a food source and wood material by providing crucial genomic resources.

关键词： bamboo shoots Dendrocalamus brandisii(Munro)Kurz flavonoid genome lignin

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含重金属Pb的磷酸钾镁水泥性能

建筑材料学报 2016年第1期19卷 29-34页

作者：徐选臣杨建明顾玉萍盐城工学院土木工程学院江苏盐城224051 江苏省生态建材与环保装备协同创新中心江苏盐城224051

测试了掺Pb(NO_3)_2的磷酸钾镁水泥(MKPC)浆体的流动度、凝结时间、抗压强度、收缩变形和重金属Pb的浸出质量浓度,分析了MKPC浆体的水化放热特性、物相组成和微观形貌.结果表明:复合缓凝剂和适量Pb(NO_3)_2可有效延缓MKPC浆体的凝结时间... 详细信息

测试了掺Pb(NO_3)_2的磷酸钾镁水泥(MKPC)浆体的流动度、凝结时间、抗压强度、收缩变形和重金属Pb的浸出质量浓度,分析了MKPC浆体的水化放热特性、物相组成和微观形貌.结果表明:复合缓凝剂和适量Pb(NO_3)_2可有效延缓MKPC浆体的凝结时间;掺入适量Pb(NO_3)_2虽然会降低MKPC浆体的抗压强度,并使其收缩变形略微增加,但掺10%(质量分数)Pb(NO_3)_2的MKPC浆体28d抗压强度仍大于20MPa,其收缩变形仍比硅酸盐水泥砂浆小1个数量级;在相同水化龄期,MKPC浆体中重金属Pb的浸出质量浓度随着Pb(NO_3)_2掺量的增加逐步升高,但在掺10%Pb(NO_3)_2的MKPC浆体中,重金属Pb的浸出质量浓度仍远低于GB 5085.3—2007标准要求.

关键词：磷酸钾镁水泥重金属Pb 抗压强度 Pb浸出质量浓度

链接与协同:产教融合“四链”有机衔接的内在逻辑

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国家教育行政学院学报 2021年第4期 48-56页

作者：张庆民顾玉萍南京财经大学管理科学与工程学院江苏南京210023 安徽财经大学管理科学与工程学院安徽蚌埠233030

推进产教融合的核心是实现高校教育链、人才链与企业产业链、创新链(简称"四链")的有机衔接。从网络链接视角分析"四链"之间的内在关联性,探究促进产教融合"四链"有机衔接的政策补链、收益强链、服务延链与生态固链的网络链接结构,通过... 详细信息

推进产教融合的核心是实现高校教育链、人才链与企业产业链、创新链(简称"四链")的有机衔接。从网络链接视角分析"四链"之间的内在关联性,探究促进产教融合"四链"有机衔接的政策补链、收益强链、服务延链与生态固链的网络链接结构,通过协同思想探讨政府赋能中的政策协同、收益协同、多主体跨界协同与标准协同机制。研究表明,领先企业产业链、创新链与高校教育链之间的有机衔接是深化产教融合、协同育人项目的关键,加强产教融合物理链接新基建和协同软实力建设是推进产教融合"四链"有机衔接的基础性保障,对化解产教鸿沟现象起到关键性作用。

关键词：产教融合 “四链” 链接机制协同机制