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Genes & Diseases 2023年第5期10卷 1809-1811页

作者： Chao Ma Yuanhua Qin Ying Wang Chuanliang Zhu Xingjie Gao Pingping Zhang yupeng gu Shuailong Zhang Jintao Lin Jiahui Wang Weifeng Mao College of Basic Medical Sciences Dalian Medical UniversityDalianLiaoning 116044China Liaoning Provincial Key Laboratory of Medical Molecular Biology DalianLiaoning 116044China

Triple-negative breast cancer(TNBC)is highly malignant and refractory to immunotherapy through impeding the immune cell infiltration and inflammation in the tumor microenvironment(TME).1 DNA-dependent protein kinase catalytic subunit(DNA-PKcs)is a member of the phosphatidylinositol 3-kinase-related kinase family,which is required for the non-homologous end joining repair.2 The effect of DNA-PKcs on the generation of cytosolic DNA and inflammation response in tumor immuno-environment is not defined.We found a specific DNA-PKcs inhibitor,NU7441,induced cytosolic DNA,stimulator of interferon genes(STING),and retinoic acid-inducible gene I(RIG-I)signals in vitro.In Balb/c immune-competent mice bearing 4T1 TNBC cells,NU7441 impaired the tumor growth and metastasis,and increased the CD45+leukocytes,CD4+T cells,CD8+T cells,and CD1a+antigen-presenting cells,as well as MHC-I and interferon alpha receptor(IFNAR)in TME.However,in Balb/c athymic nude mice without IFNAR and CD8+T cells in TME,NU7741 did not influence tumor growth.These results show that inhibition of DNA-PKcs triggers cytosolic DNA sensing and induces an inflamed TME to promote anti-tumoral immunity,which provides a strategy to alter the inflammation and lymphocyte infiltration in TME to increase the efficacy of immunotherapy in TNBC and other cancers with an immune-suppressive TME.

关键词： immunity inflammation interferon

基于Multibody的穿刺手术机器人设计与仿真

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实验室研究与探索 2024年第1期43卷 97-101页

作者：邹宇鹏张际平郑杰仁张宗源顾学斌李万哲中国石油大学(华东)机电工程学院山东青岛266580

微创穿刺手术是诊断和治疗肿瘤的重要手段。结合穿刺手术核磁兼容性和穿刺定位精度要求,提出一款2R3T并联柔索驱动核磁兼容穿刺定位手术机器人,可提高穿刺精度,缩短手术时间。设计穿刺手术机器人总体结构,完成正逆运动学分析和轨迹规划... 详细信息

微创穿刺手术是诊断和治疗肿瘤的重要手段。结合穿刺手术核磁兼容性和穿刺定位精度要求,提出一款2R3T并联柔索驱动核磁兼容穿刺定位手术机器人,可提高穿刺精度,缩短手术时间。设计穿刺手术机器人总体结构,完成正逆运动学分析和轨迹规划,借助Multibody构建机器人各模块,建立机器人运动学模型;联合运动学模型与Multibody虚拟样机模型进行仿真。仿真结果表明,运动学模型具有准确性和设计的合理性。Multibody建模仿真过程对机器人运动学和动力学仿真实验教学具有重要意义。

关键词：微创穿刺手术穿刺手术机器人建模仿真

CcpA mutants infuence selective carbon source utilization by changing interactions with target genes in Bacillus licheniformis

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Systems Microbiology and Biomanufacturing 2022年第1期2卷 193-207页

作者： yupeng Zhang Youran Li Fengxu Xiao Hanrong Wang Liang Zhang Zhongyang Ding Sha Xu Zhenghua gu guiyang Shi Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122People’s Republic of China National Engineering Laboratory for Cereal Fermentation Technology Jiangnan University1800 Lihu AvenueWuxiJiangsu Province 214122People’s Republic of China Jiangsu Provincial Research Center for Bioactive Product Processing Technology Jiangnan UniversityWuxiPeople’s Republic of China

The gram-positive bacterium Bacillus licheniformis exhibits obvious selective utilization on carbon sources.This process is mainly governed by the global regulator catabolite control protein A(CcpA),which can recognize and bind to multiple target genes that are widely distributed in metabolic pathways.Although the DNA-binding domain of CcpA has been predicted,the infuence of key amino acids on target gene recognition and binding has yet to be uncovered.In this study,the impact of Lys31,Ile42 and Leu56 on in vitro protein–DNA interactions and in vivo carbon source selective utilization was investigated.The results showed that alanine substitution of Lys31 and Ile42,located within the 3rd helices of the DNAbinding domain,signifcantly weakened the binding strength between CcpA and target genes.These mutations also lead to alleviated repression of xylose utilization in the presence of glucose.On the other hand,the Leu56Arg mutant in the 4th helices exhibited enhanced binding afnity compared with that of the wild-type one.When this mutant was used to replace the native one in B.licheniformis cells,the selective utilization of glucose over xylose increased.This study provides a new strategy for understanding the relationship between the function and structure of regulatory proteins.This study also used a new strategy was used to regulate carbon source utilization beyond CCR engineering.

关键词： CcpA Bacillus licheniformis Mutagenesis Xylose utilization

郑州地区商代建筑夯土材料的科技分析与研究——以郑州商城和郑州小双桥遗址为例

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文物保护与考古科学 2024年第1期36卷 89-100页

作者：谷天旸杨雪钦师东辉杨树刚马玉鹏郑晓旭吴金旭张玉修宋国定中国科学院脊椎动物演化与人类起源重点实验室(中国科学院古脊椎动物与古人类研究所) 北京100044 中国科学院大学人文学院考古学与人类学系北京100049 北京科技大学科技史与文化遗产研究院北京100083 河南省文物考古研究院河南郑州450000 郑州商都遗址博物院河南郑州450000 郑州市管城回族区文化旅游体育局河南郑州450000 中国科学院大学地球与行星科学学院北京100049 北京联合大学考古研究院北京100191

本研究以郑州商城和小双桥遗址发现的不同建筑类型的夯土材料(城墙、高台基址、建筑基础、水井等)为对象,利用显微观察、拉曼光谱分析、粒度分析、磁化率测定、烧失量测定等科技方法,结合形貌特征比对开展综合研究。研究初步探析了郑州... 详细信息

本研究以郑州商城和小双桥遗址发现的不同建筑类型的夯土材料(城墙、高台基址、建筑基础、水井等)为对象,利用显微观察、拉曼光谱分析、粒度分析、磁化率测定、烧失量测定等科技方法,结合形貌特征比对开展综合研究。研究初步探析了郑州地区商代各类型夯土材料的结构、组成和物性特征。通过土壤微形态分析等方法有效识别了夯土的微观结构、组分和微构造,揭示了先民夯筑行为对原生土壤的改造现象,进而探讨了夯土在建造和使用时期的人地关系。

关键词：古代建筑夯筑技术郑州商城郑州小双桥

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Ni(dmgH)_(2) complex coupled with metal-organic frameworks MIL-101(Cr)for photocatalytic H_(2) evolution under visible light irradiation

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Journal of Materiomics 2017年第1期3卷 58-62页

作者： Rong Wang Li Wu Bryant Chica Lina gu Gengsheng Xu yupeng Yuan School of Chemistry and Chemical Engineering Innovation Lab for Clean Energy&Green CatalysisAnhui UniversityHefei 230601China Department of Chemistry Emory UniversityAtlantaGA 30322United States

Metal-organic frameworks(MOFs)have been tremendously used as photocatalysts for H_(2) generation in recent years.Lacking native active sites(so-called co-catalyst)for H_(2) generation motivates the incorporation of noble metals and their molecular complexes,hydrogenase active site mimics into MOFs to promote H_(2)generation.We herein report an noble-metal-free photocatalytic H_(2) generation system consisting of Erythrosin B dye-sensitized MIL-101(Cr)as a light absorber and Ni(dmgH)_(2) as a co-catalyst.It is found that Ni(dmgH)_(2) can serve as an efficient co-catalyst to boost H_(2) generation in the presence of triethanolamine(TEOA)as an electron donor under visible light irradiation.The optimal MIL-101(Cr)/Ni(dmgH)_(2) hybrid(5 wt%Ni(dmgH)_(2))displays a hydrogen H_(2) rate of 45.5 mmol h^(-1),which is 10 times greater than the control sample without Ni(dmgH)_(2) loading.This paper provides a novel design route for active H_(2) generation systems by combining molecular complexes of earth-abundant metal and MOFs photocatalysts.

关键词： Metal-organic frameworks Ni(dmgH)_(2) Water splitting H_(2) Photocatalysis

Effects of different ridge-furrow mulching systems on yield and water use efficiency of summer maize in the Loess Plateau of China

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Journal of Arid Land 2021年第9期13卷 947-961页

作者： CHEN Pengpeng gu Xiaobo LI Yuannong QIAO Linran LI yupeng FANG Heng YIN Minhua ZHOU Changming Key Laboratory of Agricultural Soil and Water Engineering in Arid and Semiarid Areas of Ministry of Education Northwest A&F UniversityYangling 712100China College of Art and Science Georgia State UniversityAtlanta 30341USA College of Water Conservancy and Hydropower Engineering Gansu Agricultural UniversityLanzhou 730070China College of Land Resources and Environment Jiangxi Agricultural UniversityNanchang 330045China

Ridge-furrow film mulching has been proven to be an effective water-saving and yield-improving planting pattern in arid and semi-arid regions.Drought is the main factor limiting the local agricultural production in the Loess Plateau of China.In this study,we tried to select a suitable ridge-furrow mulching system to improve this situation.A two-year field experiment of summer maize(Zea mays L.)during the growing seasons of 2017 and 2018 was conducted to systematically analyze the effects of flat planting with no film mulching(CK),ridge-furrow with ridges mulching and furrows bare(RFM),and double ridges and furrows full mulching(DRFFM)on soil temperature,soil water storage(SWS),root growth,aboveground dry matter,water use efficiency(WUE),and grain yield.Both RFM and DRFFM significantly increased soil temperature in ridges,while soil temperature in furrows for RFM and DRFFM was similar to that for CK.The largest SWS was observed in DRFFM,followed by RFM and CK,with significant differences among them.SWS was lower in ridges than in furrows for RFM.DRFFM treatment kept soil water in ridges,resulting in higher SWS in ridges than in furrows after a period of no water input.Across the two growing seasons,compared with CK,RFM increased root mass by 10.2%and 19.3%at the jointing and filling stages,respectively,and DRFFM increased root mass by 7.9%at the jointing stage but decreased root mass by 6.0%at the filling stage.Over the two growing seasons,root length at the jointing and filling stages was respectively increased by 75.4%and 58.7%in DRFFM,and 20.6%and 30.2%in RFM.Relative to the jointing stage,the increased proportions of root mass and length at the filling stage were respectively 42.8%and 94.9%in DRFFM,63.2%and 115.1%in CK,and 76.7%and 132.1%in RFM,over the two growing seasons,showing that DRFFM slowed down root growth while RFM promoted root growth at the later growth stages.DRFFM treatment increased root mass and root length in ridges and decreased them in 0-30 cm soil layer,while RFM increased them in 0-30 cm s

关键词： ridge-furrow mulching summer maize soil water storage soil temperature root mass root length

In-orbit performance of ME onboard Insight-HXMT in the first 5 years

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Radiation Detection Technology and Methods 2023年第1期7卷 15-24页

作者： Ying Tan Xuelei Cao Weichun Jiang Xiaobo Li Bin Meng Wanchang Zhang Sheng Yang Tao Luo Yudong gu Liang Sun Xiaojing Liu Yuanyuan Du Jiawei Yang Yanjun Xu Jinyuan Liao yupeng Xu Fangjun Lu Liming Song Shuangnan Zhang Key Laboratory of Particle Astrophysics Institute of High Energy PhysicsChinese Academy of Science19B Yuquan RoadShijingshan DistrictBeijing 100049China

Introduction The medium-energy X-ray telescope(ME)is a collimated X-ray telescope onboard the Insight hard X-ray modulation telescope(Insight-HXMT)satellite.It has 1728 Si-PIN pixels readout using 54 low noise application-specific integrated circuits(ASICs).ME covers the energy range of 5–30 keV and has a total detection area of 952cm2.The typical energy resolution of ME at the beginning of the mission is 3 keV at 17.8 keV(full width at half maximum,FWHM),and the time resolution is 255μs.In this study,we present the in-orbit performance of ME in its first 5 years of operation.Methods The performance of ME was monitored using onboard radioactive sources and astronomical X-ray objects.ME carries six 241Am radioactive sources for onboard calibration,which can continuously illuminate the calibration pixels.The long-term performance evolution of ME can be quantified using the properties of the accumulated spectra of the calibration pixels.In addition,observations of the Crab Nebula and the pulsar were used to check the long-term evolution of the detection efficiency as a function of energy.Conclusion After 5 years of operation,742cm2 of the Si-PIN pixelswere stillworking normally.The peak positions of 241Am emission lines gradually shifted to the high-energy region,implying a slow increase in ME gain of 1.43%.A comparison of the ME spectra of the Crab Nebula and the pulsar shows that the E–C relations and the redistribution matrix file are still acceptable for most data analysis works,and there is no detectable variation in the detection efficiency.

关键词： Hard X-ray Calibration Detector In-orbit performance

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嵌入式党建降低了国有企业薪酬差距吗?

财经论丛 2023年第2期 55-67页

作者：修宗峰顾宇鹏殷敬伟中南大学商学院湖南长沙410083

新时期我国国有企业创新性地将党建工作嵌入了“公司章程”,这一制度安排实现了坚持党对国有企业的领导和建立现代企业制度“两个一以贯之”原则的稳步推进。本文手工搜集2015至2019年我国A股国有上市公司的“公司章程”数据,构建“党... 详细信息

新时期我国国有企业创新性地将党建工作嵌入了“公司章程”,这一制度安排实现了坚持党对国有企业的领导和建立现代企业制度“两个一以贯之”原则的稳步推进。本文手工搜集2015至2019年我国A股国有上市公司的“公司章程”数据,构建“党建入章”数据库,实证检验了“嵌入式党建”对国有企业薪酬差距的潜在影响。研究发现:“党建入章”能降低国有企业薪酬差距,这一作用效果在地方国有企业中更加明显。机制分析表明,在竞争类、商业类以及不具有政治关联的国企中,“党建入章”对薪酬差距的抑制作用更强。本文发现“党建入章”通过将坚持党对国有企业的领导与公司治理相融合有效降低了国有企业薪酬差距,证明“党建入章”这一制度安排对深化我国国有企业改革具有重要的理论价值和政策意义。

关键词：党建入章党组织嵌入薪酬差距国有企业

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“党建入章”、国有产权与企业财务舞弊

上海财经大学学报（哲学社会科学版） 2022年第4期24卷 93-107页

作者：修宗峰顾宇鹏殷敬伟中南大学商学院湖南长沙410083

“党建入章”旨在企业通过将党建写入公司章程来实现其基层党组织对公司治理的有效参与。文章手工搜集了2007—2019年中国A股上市公司的公司章程数据,通过构建“党建入章”数据库实证检验了“党建入章”这一基层党组织的制度安排对企业... 详细信息

“党建入章”旨在企业通过将党建写入公司章程来实现其基层党组织对公司治理的有效参与。文章手工搜集了2007—2019年中国A股上市公司的公司章程数据,通过构建“党建入章”数据库实证检验了“党建入章”这一基层党组织的制度安排对企业财务舞弊行为的潜在影响。研究发现:“党建入章”能够抑制企业财务舞弊行为,这种舞弊治理作用在企业国有产权比例较高时更加凸显;渠道分析发现,“党建入章”对企业财务舞弊行为的抑制作用能够通过强化企业内部控制制度有效性得以实现;异质性分析表明,“党建入章”对企业财务舞弊行为的抑制作用在双向进入程度较强、外部监督(如审计师、分析师关注以及监管距离)相对较弱时更加明显。文章从企业财务舞弊治理的角度证明了“党建入章”对完善企业监督机制、提升公司治理效率的积极效果,既为国有企业“党建入章”这一制度安排的合理性和必要性奠定了理论基础,也为继续完善党组织嵌入公司治理的实践思路提供了政策启示。

关键词：党建入章党组织嵌入企业财务舞弊国有产权

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贵州不同产地冬荪营养成分及品质评价

中国农业科技导报 2023年第11期25卷 143-153页

作者：刘宇鹏陈芳古书鸿王芳贵州省山地环境气候研究所贵州省山地气候与资源重点实验室贵阳550002 贵州科学院贵州省生物研究所贵阳550009

为系统全面地分析贵州冬荪营养成分和品质差异,以4个产地冬荪干品为材料,测定其氨基酸、矿质元素、灰分、脂肪、粗纤维和总糖含量,通过氨基酸比值系数法对其蛋白质品质进行评价,并利用主成分分析法对不同产地冬荪品质进行综合评价。结... 详细信息

为系统全面地分析贵州冬荪营养成分和品质差异,以4个产地冬荪干品为材料,测定其氨基酸、矿质元素、灰分、脂肪、粗纤维和总糖含量,通过氨基酸比值系数法对其蛋白质品质进行评价,并利用主成分分析法对不同产地冬荪品质进行综合评价。结果表明,冬荪中N、P、K和Ca、Cu、Fe、Mg、Zn 8种矿质元素含量分别为18.70~22.45、3.55~4.83、29.63~39.64g·kg^(-1)和508.64~686.89、12.37~17.54、92.30~497.54、1.55~1.98、24.84~51.61 mg·kg-1;灰分、脂肪、粗纤维和总糖含量分别为5.83%~6.27%、0.83%~1.49%、0.25%~0.47%、28.92%~34.90%。不同产地冬荪均含有被检测的17种氨基酸,其中谷氨酸的含量最高,必需氨基酸含量为4.24%~5.55%,总氨基酸含量为10.70%~12.93%,以上指标都以德江产的冬荪最高。氨基酸评价结果表明,赖氨酸是冬荪的第一限制性氨基酸,4个产地冬荪必需氨基酸指数均大于1,可作为优质蛋白源,氨基酸比值系数分值表明,德江产冬荪蛋白质营养价值最高。主成分分析结果表明,德江产冬荪综合得分最高,品质最佳。研究结果为贵州省冬荪质量控制和开发利用提供科学依据。

关键词：冬荪营养成分品质评价氨基酸比值系数主成分分析