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Co-delivery of luteolin and TGF-β1 plasmids with ROS-responsive virus-inspired nanoparticles for microenvironment regulation and chemo-gene therapy of intervertebral disc degeneration

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Nano Research 2022年第9期15卷 8214-8227页

作者： Yifan Ding Huan wang yunyun wang Long Li Jiahui Ding Caiyan Yuan Tao Xu Haoran Xu Hui Xie Ning Zhu Xin Hu Huang Fang Songwei Tan Department of Orthopedic Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China School of Pharmacy Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Cardiology the Fifth Hospital in WuhanWuhan 430030China Department of Radiology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China College of Biotechnology and Pharmaceutical Engineering Nanjing Tech UniversityNanjing 211800China State Key Laboratory of Materials-Oriented Chemical Engineering Nanjing Tech UniversityNanjing 211800China College of Materials Science and Engineering Nanjing Tech UniversityNanjing 211800China

Intervertebral disc degeneration(IDD)is closely related to inflammation and imbalance of synthesis/catabolism of extracellular matrix(ECM)in intervertebral disc(IVD).Considering this,luteolin(LUT),a kind of natural flavonoid with good anti-inflammatory effect and TGF-β1(a gene that promotes the regeneration of ECM)plasmid was co-loaded and co-delivered to nucleus pulposus cells(NPCs).Reactive oxygen species(ROS)responsive cationic copolymer,poly(β-amino ester)-poly(ε-caprolactone)(PBC),with high plasmid DNA(pDNA)compression affinity was *** can self-assemble into nano-sized polyplexes(pDNA@PBC)with virus-inspired structure and function through which it can transfect pDNA into NPCs with very high efficiency and negligible *** was encapsulated in the hydrophobic core of pDNA@*** co-delivery system,LUT-pTGFβ1@PBC,could enhance the cellular uptake of NPCs and manifest excellent sustained drug release in *** time quantitative polymerase chain reaction(RT-qPCR)and Western blot experiments reveal that the co-delivery system could inhibit inflammation in NPCs and restore the balance of anabolism and catabolism in vitro by activating TGF/SMAD3 and inhibiting NFkB/***,LUT-pTGF-β1@PBC retards IDD in vivo as detected by radiological and histological methods with good biosafety in ***-pTGF-β1@PBC may be a promising option for the treatment of IDD.

关键词： intervertebral disc degeneration poly(β-amino ester) luteolin TGF-β1 co-delivery

Knockout of C6orf120 in Rats Alleviates Concanavalin A-induced Autoimmune Hepatitis by Regulating Macrophage Polarization

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Biomedical and Environmental Sciences 2024年第6期37卷 594-606页

作者： Xin wang Yuqi wang Hui Liu Yingying Lin Peng wang yunyun Yi Xin Li Department of Center of Integrated Traditional Chinese and Western Medicine Peking University Ditan Teaching HospitalBeijing 100015China Department of Center of Integrated Traditional Chinese and Western Medicine Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China Department of Center of Infectious Disease Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China

Objective The effect of the functionally unknown gene C6orf120 on autoimmune hepatitis was investigated on C6orf120 knockout rats(C6orf120^(-/-))and THP-1 *** Six–eight-week-old C6orf120^(-/-)and wild-type(WT)SD rats were injected with Con A(16 mg/kg),and euthanized after 24 *** sera,livers,and spleens were ***-1 cells and the recombinant protein(rC6ORF120)were used to explore the mechanism in *** frequency of M1 and M2 macrophages was analyzed using flow *** blotting and PCR were used to detect macrophage polarization-associated *** C6orf120 knockout attenuated Con A-induced autoimmune *** cytometry indicated that the proportion of CD68^(+)CD86^(+)M1 macrophages from the liver and spleen in the C6orf120^(-/-)rats decreased.C6orf120 knockout induced downregulation of CD86 protein and the mRNA levels of related inflammatory factors TNF-α,IL-1β,and IL-6 in the liver.C6orf120 knockout did not affect the polarization of THP-1 ***,rC6ORF120 promoted the THP-1 cells toward CD68^(+)CD80^(+)M1 macrophages and inhibited the CD68^(+)CD206^(+)M2 *** C6orf120 knockout alleviates Con A-induced autoimmune hepatitis by inhibiting macrophage polarization toward M1 macrophages and reducing the expression of related inflammatory factors in C6orf120^(-/-)rats.

关键词： C6orf120 Autoimmune hepatitis Macrophage polarization M1 macrophages

Recent advances in the study of sepsis-induced depression

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Journal of Intensive Medicine 2023年第3期3卷 239-243页

作者： yunyun wang Youjia Zhu Mi Tian Yao wang Xu Pei Junliang Jiang Yu He Ye Gong Department of Critical Care Medicine and Neurosurgery of Huashan Hospital State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for BrainScienceInstitutes of Brain ScienceFudan UniversityShanghai 200000China

Progress in medicine such as the use of anti-infective drugs and development of the advanced life support equipment has greatly improved the survival rate of patients with ***,the incidence of sepsis-relateddiseases is *** include severe neurologic and psychologic disorders,cognitive decline,anxiety,depression,and post-traumatic stress *** dysfunction occurs via multiple interacting mechanisms,with different causative pathogens having distinct *** sepsis-related diseases place a substantial burden on patients and their families,it is important to elucidate the underlying pathophysiologic mechanisms todevelop effective treatments.

关键词： Sepsis Sepsis-associated encephalopathy Depression Mechanism

New fossil caddisfly from Middle Jurassic of Daohugou,Inner Mongolia,China(Trichoptera:Philopotamidae)

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Progress in Natural Science:Materials International 2009年第10期19卷 1427-1431页

作者： Meixia wang,yunyun Zhao,Dong Ren College of Life Science,Capital Normal University,Beijing 100037,China College of Life Science Capital Normal University Beijing 100037 China

A new genus and new species,Juraphilopotamus lubricus *** ***.,from the Middle Jurassic Jiulongshan Formation of Daohugou,Inner Mongolia,China,is described and *** may be the first record of the family Philopotamidae in China,extending the geographic distribution of this family.A detailed description and illustration of the specimen along with a brief review of the fossil Philopotamidae are given.A proposal on dispersionandmigration of Philopotamidae and problems of the paleoenvironment of the Daohugou beds are discussed.

关键词： Insecta Juraphilopotamus Philopotamidae Middle Jurassic Biogeography

Self-corrected unsupervised domain adaptation

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Frontiers of Computer Science 2022年第5期16卷 35-43页

作者： yunyun wang Chao wang Hui XUE Songcan CHEN School of Computer Science and Engineering Nanjing University of Posts&TelecommunicationsNanjing 210046China School of Computer Science and Engineering Southeast UniversityNanjing 210096China School of Computer Science and Technology Nanjing University of Aeronautics and AstronauticsNanjing 210023China

Unsupervised domain adaptation(UDA),which aims to use knowledge from a label-rich source domain to help learn unlabeled target domain,has recently attracted much *** methods mainly concentrate on source classification and distribution alignment between domains to expect the correct target *** in this paper,we attempt to learn the target prediction end to end directly,and develop a Self-corrected unsupervised domain adaptation(SCUDA)method with probabilistic label *** adopts a probabilistic label corrector to learn and correct the target labels ***,besides model parameters,those target pseudo-labels are also updated in learning and corrected by the anchor-variable,which preserves the class candidates for *** on real datasets show the competitiveness of SCUDA.

关键词： unsupervised domain adaptation adversarial Learning deep neural network pseudo-labels label corrector

Tropical cyclone genesis over the western north Pacific in La Ni?a decay summers: Comparison between 2018 and 2021

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Science China Earth Sciences 2024年第10期67卷 3098-3109页

作者： yunyun LIU Zhensong GONG China Meteorological Administration Key Laboratory for Climate Prediction Studies National Climate Centre Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters Nanjing University of Information Science &Technology

As the primary interannual signal of variability in the tropical ocean-atmosphere interaction, the El Ni?o-Southern Oscillation has a considerable impact on tropical cyclone(TC) activity over the western North Pacific(WNP). Both 2018 and2021 were La Ni?a decay years, but TC activity over the WNP during the two summers(June–August) showed notable differences. In 2018, summer TC activity was unusually high with a total of 18 TCs, and the region of TC genesis was mainly in the central and eastern WNP. In contrast, only 9 TCs were generated in summer 2021, and the region of TC genesis was primarily in the western WNP. By comparing the characteristics of the large-scale environmental conditions over the regions of TC genesis, the thermal factors of the tropical oceans, and the activity of the Madden-Julian Oscillation(MJO), this study revealed the possible causes for the marked differences in TC genesis over the WNP during the two summers, which both had a similar background of La Ni?a decay. The Indian Ocean Basin Mode(IOBM) transitioned of a cold anomaly in the winter of 2017/2018and persisted until summer 2018. At the same time, the Pacific Meridional Mode(PMM) maintained a positive phase, leading to eastward and northward displacement of the Western Pacific Subtropical High in summer, and eastward extension of the tropical monsoon trough, which presented conditions conducive to TC genesis over the Northwest Pacific. Moreover, the days when the MJO stagnated in phases 5 and 6 in the summer of 2018 increased by approximately 150% relative to climatological state,providing dynamic conditions favorable for TC formation. In 2021, the IOBM quickly turned to a warm anomaly in March and persisted until summer, whereas the PMM became a negative phase in January and remained so until summer. At the same time,the MJO stagnated in phases 2 and 3 for up to 47 days, with the center of convection located over the western Maritime Continent, producing conditions unconducive to TC ge

关键词： Tropical cyclone La Niña events Indian Ocean Basin Mode Pacific Meridional Mode Madden-Julian Oscillation Western Pacific Subtropical High Monsoon trough

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循证医学类课程对医学研究生批判性思维能力的培养效果

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中华医学教育杂志 2024年第3期44卷 217-221页

作者：阎思宇靳英辉王宇王云云黄桥王永博黄笛郭毅曾宪涛武汉大学中南医院循证与转化医学中心武汉430071

目的调查循证医学类课程对医学研究生批判性思维能力的培养效果。方法采用问卷调查方法。2020年9至11月,以武汉大学2020级学习循证医学和循证临床实践课程(循证医学类课程)的医学研究生为调查对象,采用中文版批判性思维能力测量表分别... 详细信息

目的调查循证医学类课程对医学研究生批判性思维能力的培养效果。方法采用问卷调查方法。2020年9至11月,以武汉大学2020级学习循证医学和循证临床实践课程(循证医学类课程)的医学研究生为调查对象,采用中文版批判性思维能力测量表分别于课程前、课程后对医学研究生的批判性思维能力进行调查。采用Wilcoxon符号秩检验、Wilcoxon秩和检验和Kruskal-Wallis检验进行数据分析。结果共回收有效问卷331份。课程后医学研究生批判性思维能力总分高于课程前[297.0(45.0)分比291.0(40.0)分,P=0.001]。课程后医学研究生的寻找真相特质、系统化能力特质、批判思维的自信心特质评分均高于课程前[40.0(9.0)分比38.0(7.0)分,41.0(9.0)分比40.0(8.0)分,39.0(6.0)分比39.0(6.0)分,均P<0.05]。课程前参与科研项目的医学研究生在循证医学类课程前后批判性思维能力评分的变化值高于未参与科研项目者(6.0分比1.5分,P=0.032)。结论循证医学类课程可以提升医学研究生的批判性思维能力,且课前参与科研项目有助于进一步提升循证医学类课程对医学研究生批判性思维能力的培养效果。

关键词：循证医学循证临床实践批判性思维医学研究生教学效果科研参与

贵阳市社区老年人死亡认知及影响因素的质性研究

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贵州医药 2024年第7期48卷 1034-1037页

作者：王先美刘志燕邓婕王芸芸熊吴燕邹惠美肖霓贵州医科大学护理学院贵州贵阳561113 贵州医科大学地方病与少数民族疾病教育部重点实验室贵州贵阳561113

目的探讨社区老年人对死亡的认知及影响因素。方法运用描述性现象学研究方法,采用最大差异选样,从不同文化层次进行抽样,资料饱和后再增加2例,最终纳入11名社区老年人进行面对面半结构式访谈,对所得资料采用内容分析法及主题分析法,编... 详细信息

目的探讨社区老年人对死亡的认知及影响因素。方法运用描述性现象学研究方法,采用最大差异选样,从不同文化层次进行抽样,资料饱和后再增加2例,最终纳入11名社区老年人进行面对面半结构式访谈,对所得资料采用内容分析法及主题分析法,编码采用手工编码和Nvivo 12软件相结合的方式。结果贵阳市社区老年人的死亡认知归纳为:回避死亡;被动的应对死亡,表现为应对死亡的无力感、无助感;对死亡过程未知性、不可预见性充满恐惧感;优死的需求在生理上主要表现在身体少受痛苦,心理上主要表现为追求内心的宁静和对家人关爱与陪伴的渴求;欠和睦的家庭关系、薄弱的家庭经济、争强好胜的性格特质、身体状况的好坏和年纪的大小是影响老年人死亡态度的重要因素。结论在健康老龄化背景下,应在社区开展死亡教育,引导老年人关注“优死”,帮助老年人更好地理解生命的意义、树立正确的生死观,打破死亡的神秘色彩,减轻老年人应对死亡的自我感受负担,以更加积极的心态应对死亡。

关键词：社区老年人死亡态度生命质性研究

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复方苍术挥发油微乳的制备工艺研究

广东化工 2024年第7期51卷 49-51,10页

作者：李睿刘謦蔚王芸芸沈佳伟万芳荆楚理工学院湖北荆门448000

目的:对复方苍术挥发油微乳的处方进行筛选,考察其制备工艺条件。方法:根据复方苍术挥发油与油相、乳化剂、助乳化剂的互溶现象进行初步筛选,然后通过绘制伪三元相图,综合考虑微乳区域面积、微乳的外观、稳定性,最终确定处方组成,并在... 详细信息

目的:对复方苍术挥发油微乳的处方进行筛选,考察其制备工艺条件。方法:根据复方苍术挥发油与油相、乳化剂、助乳化剂的互溶现象进行初步筛选,然后通过绘制伪三元相图,综合考虑微乳区域面积、微乳的外观、稳定性,最终确定处方组成,并在此基础上进一步对微乳的制备温度、搅拌速度、搅拌时间进行比较研究,测定微乳粒径。结果:复方苍术挥发油微乳的制备工艺为:将EL-40与异丙醇按3∶1的质量比混合,制得混合乳化剂,再取等质量复方苍术挥发油与油酸乙酯混合为油相,将混合乳化剂与油相按8∶2的质量比混合,在300 r/min,30±5℃条件下,加入蒸馏水搅拌10~15 min至澄清透明的微乳形成。微乳平均粒径为20.71 nm,分散系数为0.019。结论:复方苍术挥发油微乳的制备工艺简单可行,微乳澄清透明,粒径小、分布均匀,稳定性良好。

关键词：复方苍术挥发油微乳伪三元相图稳定性处方组成制备工艺