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Journal of Pharmaceutical Analysis 2024年第3期14卷 371-388页

作者： Haonan Ruan Jing Zhang yunyun wang Ying Huang Jiashuo Wu Chunjiao He Tongwei Ke Jiaoyang Luo Meihua Yang Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine Ministry of EducationInstitute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing100193China

Zearalenone(ZEN)is a mycotoxin that extensively contaminates food and feed,posing a significant threat to public health.However,the mechanisms behind ZEN-induced intestinal immunotoxicity remain unclear.In this study,Sprague-Dawley(SD)rats were exposed to ZEN at a dosage of 5 mg/kg/day b.***.for a duration of 14 days.The results demonstrated that ZEN exposure led to notable pathological alterations and immunosuppression within the intestine.Furthermore,ZEN exposure caused a significant reduction in the levels of apolipoprotein E(ApoE)and liver X receptor(LXR)(P<0.05).Conversely,it upregulated the levels of myeloid-derived suppressor cells(MDSCs)markers(P<0.05)and decreased the presence of 27-hydroxycholesterol(27-HC)in the intestine(P<0.05).It was observed that ApoE or LXR agonists were able to mitigate the immunosuppressive effects induced by ZEN.Additionally,a bioinformatics analysis highlighted that the downregulation of ApoE might elevate the susceptibility to colorectal,breast,and lung cancers.These findings underscore the crucial role of the 27-HC/LXR/ApoE axis disruption in ZEN-induced MDSCs proliferation and subsequent inhibition of T lymphocyte activation within the rat intestine.Notably,ApoE may emerge as a pivotal target linking ZEN exposure to cancer development.

关键词： Zearalenone Intestinal immunosuppression Apolipoprotein E Bioinformatics analysis Cancer

Dynamics and genetic regulation of macronutrient concentrations during grain development in maize

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Journal of Integrative Agriculture 2024年第3期23卷 781-794页

作者： Pengcheng Li Shuangyi Yin yunyun wang Tianze Zhu Xinjie Zhu Minggang Ji Wenye Rui Houmiao wang Chenwu Xu Zefeng Yang Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Agricultural CollegeYangzhou UniversityYangzhou 225009China Jiangsu Co-lnnovation Center for Modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009China

Nitrogen(N), phosphorus(P), and potassium(K) are essential macronutrients that are crucial not only for maize growth and development, but also for crop yield and quality. The genetic basis of macronutrient dynamics and accumulation during grain filling in maize remains largely unknown. In this study, we evaluated grain N, P, and K concentrations in 206 recombinant inbred lines generated from a cross of DH1M and T877 at six time points after pollination. We then calculated conditional phenotypic values at different time intervals to explore the dynamic characteristics of the N, P, and K concentrations. Abundant phenotypic variations were observed in the concentrations and net changes of these nutrients. Unconditional quantitative trait locus(QTL) mapping revealed 41 non-redundant QTLs, including 17, 16, and 14 for the N, P, and K concentrations, respectively. Conditional QTL mapping uncovered 39 non-redundant QTLs related to net changes in the N, P, and K concentrations. By combining QTL, gene expression, co-expression analysis, and comparative genomic data, we identified 44, 36, and 44 candidate genes for the N, P, and K concentrations, respectively, including GRMZM2G371058 encoding a Doftype zinc finger DNA-binding family protein, which was associated with the N concentration, and GRMZM2G113967encoding a CBL-interacting protein kinase, which was related to the K concentration. The results deepen our understanding of the genetic factors controlling N, P, and K accumulation during maize grain development and provide valuable genes for the genetic improvement of nutrient concentrations in maize.

关键词： maize nutrient concentration unconditional QTL mapping conditional QTL mapping dynamic trait

Stay in touch with the endoplasmic reticulum

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Science China(Life Sciences) 2024年第2期67卷 230-257页

作者： Sha Sun Gan Zhao Mingkang Jia Qing Jiang Shulin Li Haibin wang Wenjing Li yunyun wang Xin Bian Yan G.Zhao Xun Huang Ge Yang Huaqing Cai Jose C.Pastor-Pareja Liang Ge Chuanmao Zhang Junjie Hu National Laboratory of Biomacromolecules Institute of BiophysicsUniversity of Chinese Academy of SciencesChinese Academy of SciencesBeijing 100101China The Ministry of Education Key Laboratory of Cell Proliferation and Differentiation College of Life SciencesPeking UniversityBeijing 100871China State Key Laboratory of Membrane Biology Tsinghua-Peking Center for Life SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China Laboratory of Computational Biology&Machine Intelligence School of Artificial IntelligenceUniversity of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Medicinal Chemical Biology College of Life SciencesNankai UniversityTianjin 300071China Brain Research Center Department of BiologySchool of Life SciencesSouthern University of Science and TechnologyShenzhen 518055China State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China Institute of Neurosciences Consejo Superior de Investigaciones Científicas-Universidad Miguel HernándezSan Juan de Alicante 03550Spain

The endoplasmic reticulum(ER),which is composed of a continuous network of tubules and sheets,forms the most widely distributed membrane system in eukaryotic cells.As a result,it engages a variety of organelles by establishing membrane contact sites(MCSs).These contacts regulate organelle positioning and remodeling,including fusion and fission,facilitate precise lipid exchange,and couple vital signaling events.Here,we systematically review recent advances and converging themes on ER-involved organellar contact.The molecular basis,cellular influence,and potential physiological functions for ER/nuclear envelope contacts with mitochondria,Golgi,endosomes,lysosomes,lipid droplets,autophagosomes,and plasma membrane are summarized.

关键词： endoplasmic reticulum nuclear envelope mitochondria Golgi apparatus endosome lysosome lipid droplets autophagosome plasma membrane membrane contact site

Advances in the knowledge on the role of apoptosis repressor with caspase recruitment domain in hemorrhagic stroke

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Journal of Intensive Medicine 2023年第2期3卷 138-143页

作者： Xu Pei Mi Tian Yao wang Yuewen Xin Junliang Jiang yunyun wang Ye Gong Department of Critical Care Medicine Huashan HospitalFudan UniversityShanghai 200040China Department of Neurosurgery Huashan HospitalFudan UniversityShanghai 200040China

The apoptosis repressor with caspase recruitment domain(ARC)plays a critical role in extrinsic apoptosis initiation via death receptor ligands,physiological stress,infection response in a tissue-dependent manner,endoplasmic reticulum(ER)stress,genotoxic drugs,ionizing radiation,oxidative stress,and hypoxia.Recent studies have suggested that regulating apoptosis-related pathways can improve outcomes for patients with neurological diseases,such as hemorrhagic stroke.ARC expression is significantly correlated with acute cerebral hemorrhage.However,the mechanism by which it mediates the anti-apoptosis pathway remains poorly known.Here,we discuss the function of ARC in hemorrhagic stroke and argue that it could serve as an effective target for the treatment of hemorrhagic stroke.

关键词： Apoptosis repressor with caspase recruitment domain(ARC) Hemorrhagic stroke Neuroinflammation Neuronal apoptosis

Recent advances in the study of sepsis-induced depression

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Journal of Intensive Medicine 2023年第3期3卷 239-243页

作者： yunyun wang Youjia Zhu Mi Tian Yao wang Xu Pei Junliang Jiang Yu He Ye Gong Department of Critical Care Medicine and Neurosurgery of Huashan Hospital State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for BrainScienceInstitutes of Brain ScienceFudan UniversityShanghai 200000China

Progress in medicine such as the use of anti-infective drugs and development of the advanced life support equipment has greatly improved the survival rate of patients with sepsis.However,the incidence of sepsis-relateddiseases is increasing.These include severe neurologic and psychologic disorders,cognitive decline,anxiety,depression,and post-traumatic stress disorder.Cerebral dysfunction occurs via multiple interacting mechanisms,with different causative pathogens having distinct effects.Because sepsis-related diseases place a substantial burden on patients and their families,it is important to elucidate the underlying pathophysiologic mechanisms todevelop effective treatments.

关键词： Sepsis Sepsis-associated encephalopathy Depression Mechanism

Co-delivery of luteolin and TGF-β1 plasmids with ROS-responsive virus-inspired nanoparticles for microenvironment regulation and chemo-gene therapy of intervertebral disc degeneration

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Nano Research 2022年第9期15卷 8214-8227页

作者： Yifan Ding Huan wang yunyun wang Long Li Jiahui Ding Caiyan Yuan Tao Xu Haoran Xu Hui Xie Ning Zhu Xin Hu Huang Fang Songwei Tan Department of Orthopedic Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China School of Pharmacy Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Cardiology the Fifth Hospital in WuhanWuhan 430030China Department of Radiology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China College of Biotechnology and Pharmaceutical Engineering Nanjing Tech UniversityNanjing 211800China State Key Laboratory of Materials-Oriented Chemical Engineering Nanjing Tech UniversityNanjing 211800China College of Materials Science and Engineering Nanjing Tech UniversityNanjing 211800China

Intervertebral disc degeneration(IDD)is closely related to inflammation and imbalance of synthesis/catabolism of extracellular matrix(ECM)in intervertebral disc(IVD).Considering this,luteolin(LUT),a kind of natural flavonoid with good anti-inflammatory effect and TGF-β1(a gene that promotes the regeneration of ECM)plasmid was co-loaded and co-delivered to nucleus pulposus cells(NPCs).Reactive oxygen species(ROS)responsive cationic copolymer,poly(β-amino ester)-poly(ε-caprolactone)(PBC),with high plasmid DNA(pDNA)compression affinity was synthesized.It can self-assemble into nano-sized polyplexes(pDNA@PBC)with virus-inspired structure and function through which it can transfect pDNA into NPCs with very high efficiency and negligible cytotoxicity.LUT was encapsulated in the hydrophobic core of pDNA@PBC.The co-delivery system,LUT-pTGFβ1@PBC,could enhance the cellular uptake of NPCs and manifest excellent sustained drug release in IVD.Real time quantitative polymerase chain reaction(RT-qPCR)and Western blot experiments reveal that the co-delivery system could inhibit inflammation in NPCs and restore the balance of anabolism and catabolism in vitro by activating TGF/SMAD3 and inhibiting NFkB/p65.Moreover,LUT-pTGF-β1@PBC retards IDD in vivo as detected by radiological and histological methods with good biosafety in rats.LUT-pTGF-β1@PBC may be a promising option for the treatment of IDD.

关键词： intervertebral disc degeneration poly(β-amino ester) luteolin TGF-β1 co-delivery

A case of ischemic-hypoxic encephalopathy due to oral succinylcholine ingestion

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Chronic Diseases and Translational Medicine 2022年第2期8卷 145-148页

作者： yunyun wang Wenjie Shang Huafu Ni Huaiwu Yuan Yujie Su Wenping Gong Guoping Peng Department of Neurology First Affiliated Hospital of School of MedicineZhejiang UniversityHangzhouZhejiang 310003China Department of Neurology Shengzhou People's HospitalShaoxingZhejiang 312400China Department of Neurology Beilun People's HospitalNingboZhejiang 315826China

Dear Editor,Cases of succinylcholine poisoning are rarely seen in clinic,and severe brain damage due to ingestion of succinylcholine is even rarer.Here,we report on a patient who poisoned herself via oral ingestion of succinylcholine,discuss the clinical characteristics,pathogenesis,and treatment of the succinylcholine-related ischemic-hypoxic encephalopathy that this patient suffered,and review the relevant literature.This study aimed to improve clinicians'understanding of ischemic-hypoxic encephalopathy due to succinylcholine chloride toxicity.

关键词： encephalopathy hypoxic clinical

Self-corrected unsupervised domain adaptation

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Frontiers of Computer Science 2022年第5期16卷 35-43页

作者： yunyun wang Chao wang Hui XUE Songcan CHEN School of Computer Science and Engineering Nanjing University of Posts&TelecommunicationsNanjing 210046China School of Computer Science and Engineering Southeast UniversityNanjing 210096China School of Computer Science and Technology Nanjing University of Aeronautics and AstronauticsNanjing 210023China

Unsupervised domain adaptation(UDA),which aims to use knowledge from a label-rich source domain to help learn unlabeled target domain,has recently attracted much attention.UDA methods mainly concentrate on source classification and distribution alignment between domains to expect the correct target prediction.While in this paper,we attempt to learn the target prediction end to end directly,and develop a Self-corrected unsupervised domain adaptation(SCUDA)method with probabilistic label correction.SCUDA adopts a probabilistic label corrector to learn and correct the target labels directly.Specifically,besides model parameters,those target pseudo-labels are also updated in learning and corrected by the anchor-variable,which preserves the class candidates for samples.Experiments on real datasets show the competitiveness of SCUDA.

关键词： unsupervised domain adaptation adversarial Learning deep neural network pseudo-labels label corrector

SARS-CoV-2 infection induces testicular injury in Rhesus macaque

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Virologica Sinica 2022年第6期37卷 934-937页

作者： Mengying Liang Wanbo Tai yunyun wang Yulong Dai Yanfeng Yao Juan Min Yiwu Zhou Liang Liu Chao Shan Han Xia Zhiming Yuan Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyChinese Academy of SciencesWuhan430061China University of Chinese Academy of Sciences Beijing100049China Institute of Infectious Diseases Shenzhen Bay LaboratoryShenzhen518069China Department of Forensic Medicine Tongji Medical College of Huazhong University of Science and TechnologyWuhan430030China Wuhan National Biosafety Laboratory Chinese Academy of SciencesWuhan430073China The Core Facility and Technical Support Wuhan Institute of VirologyChinese Academy of SciencesWuhan430061China State Key Laboratory of Virology Wuhan Institute of VirologyChinese Academy of SciencesWuhan430061 China

Dear Editor,COVID-19 is caused by the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),which is the seventh coronavirus known to infect humans.The COVID-19 pandemic has spread to more than 200countries,with more than 600 million confirmed cases and over 6.5million deaths as of September 2022,posing a huge challenge to global public health.

关键词： acute respiratory infection

A primal perspective for indefinite kernel SVM problem

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Frontiers of Computer Science 2020年第2期14卷 349-363页

作者： Hui XUE Haiming XU Xiaohong CHEN yunyun wang School of Computer Science and Engineering Southeast UniversityNanjing 210096China Key Laboratory of Computer Network and Information Integration(Southeast University) MOENanjing 210096China College of Science Nanjing University of Aeronautics and AstronauticsNanjing 210016China School of Computer Science Nanjing University of Posts and TelecommunicationsNanjing 210046China

Indefinite kernel support vector machine(IKSVM)has recently attracted increasing attentions in machine learning.Since IKSVM essentially is a non-convex problem,existing algorithms either change the spectrum of indefinite kernel directly but risking losing some valuable information or solve the dual form of IKSVM whereas suffering from a dual gap problem.In this paper,we propose a primal perspective for solving the problem.That is,we directly focus on the primal form of IKSVM and present a novel algorithm termed as IKSVM-DC for binary and multi-class classification.Concretely,according to the characteristics of the spectrum for the indefinite kernel matrix,IKSVM-DC decomposes the primal function into the subtraction of two convex functions as a difference of convex functions(DC)programming.To accelerate convergence rate,IKSVM-DC combines the classical DC algorithm with a line search step along the descent direction at each iteration.Furthermore,we construct a multi-class IKSVM model which can classify multiple classes in a unified form.A theoretical analysis is then presented to validate that IKSVM-DC can converge to a local minimum.Finally,we conduct experiments on both binary and multi-class datasets and the experimental results show that IKSVM-DC is superior to other state-of-the-art IKSVM algorithms.

关键词： indefinite kernel support vector machine multi-class classification non-convex optimization