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Adenosine Diphosphate Ribosylation Factor-GTPase-Activating Protein Stimulates the Transport of AUX1 Endosome,Which Relies on Actin Cytoskeletal Organization in Rice Root Development

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Journal of Integrative Plant Biology 2011年第9期53卷 698-709页

作者： Cheng Du yunyuan xu Yingdian Wang Kang Chong Key Laboratory of Photosynthesis and Molecular Environmental Physiology Institute of Botanythe Chinese Academy of SciencesBeijing 100093China College of Life Sciences Beijing Normal UniversityBeijing 100875China

Polar auxin transport,which depends on polarized subcellular distribution of AUXIN RESISTANT 1/LIKE AUX1（AUX1/LAX） influx carriers and PIN-FORMED（PIN） efflux carriers,mediates various processes of plant growth and development.Endosomal recycling of PIN1 is mediated by an adenosine diphosphate（ADP）ribosylation factor（ARF）-GTPase exchange factor protein,GNOM.However,the mediation of auxin influx carrier recycling is poorly understood.Here,we report that overexpression of OsAGAP,an ARF-GTPase-activating protein in rice,stimulates vesicle transport from the plasma membrane to the Golgi apparatus in protoplasts and transgenic plants and induces the accumulation of early endosomes and AUX1.AUX1 endosomes could partially colocalize with FM4-64 labeled early endosome after actin disruption.Furthermore,OsAGAP is involved in actin cytoskeletal organization,and its overexpression tends to reduce the thickness and bundling of actin filaments.Fluorescence recovery after photobleaching analysis revealed exocytosis of the AUX1 recycling endosome was not affected in the OsAGAP overexpression cells,and was only slightly promoted when the actin filaments were completely disrupted by Lat B.Thus,we propose that AUX1 accumulation in the OsAGAP overexpression and actin disrupted cells may be due to the fact that endocytosis of the auxin influx carrier AUX1 early endosome was greatly promoted by actin cytoskeleton disruption.

关键词： actin adenosine diphosphate ribosylation factor-GTPase-activating protein AUX1 endosome polar auxin transport.

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Transcriptome-wide Analysis Of Vernalization Reveals Conserved and Species-specific Mechanisms in Brachypodium

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Journal of Integrative Plant Biology 2013年第8期55卷 696-709页

作者： Qing Huan Zhiwei Mao Jingyu Zhang yunyuan xu Kang Chong Key Laboratory of Plant Molecular Physiology Institute of Botanythe Chinese Academy of Sciences University of Chinese Academy of Sciences the Chinese Academy of Sciences

Several temperate cereals need vernalization to promote flowering. Little, however, is known about the vernalization-memory-related genes, and almost no comparative analysis has been performed. Here, RNA- Seq was used for transcriptome analysis in non-vernalized, vernalized and post-vernalized Brachypodium distachyon （L.） Beauv. seedlings. In total, the expression of 1,665 genes showed significant changes （fold change 〉4） in response to vernalization. Among them, 674 putative vernalization-memory-related genes with a constant response to vernalization were significantly enriched in transcriptional regulation and monooxygenase-mediated biological processes. Comparative analysis of vernalization-memory-related genes with barley demonstrated that the oxidative-stress response was the most conserved pathway between these two plant species. Moreover, Brachypodium preferred to regulate transcription and protein phosphorylation processes, while vernalization-memory-related genes, whose products are cytoplasmic membrane-bound-vesicle-located proteins, were preferred to be regulated in barley. Correlation analysis of the vernalization-related genes with barley revealed that the vernalization mechanism was conserved between these two plant species. In summary, vernalization, including its memory mechanism, is conserved between Brachypodium and barley, although several species-specific features also exist. The data reported here will provide primary resources for subsequent functional research in vernalization.

关键词： Brachypodium comparative analysis RNA-Seq vernalization.

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The vernalization-induced long non-coding RNA VAS functions with the transcription factor TaRF2b to promote TaVRN1 expression for flowering in hexaploid wheat

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Molecular Plant 2021年第9期14卷 1525-1538页

作者： Shujuan xu Qi Dong Min Deng Dexing Lin Jun Xiao Peilei Cheng Lijing Xing Yuda Niu Caixia Gao Wenhao Zhang yunyuan xu Kang Chong Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Beijing 100049China Innovation Academy for Seed Design Chinese Academy of SciencesBeijing 100093China State Key Laboratory of Vegetation and Environmental Change Institute of BotanyChinese Academy of SciencesBeijing 100093China State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China State Key Laboratory of Plant Cell and Chromosome Engineering Center for Genome EditingInstitute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China College of Horticulture Nanjing Agricultural UniversityKey Laboratory of LandscapingMinistry of AgricultureNanjing 210095China CAS-JIC Centre of Excellence for Plant and Microbial Science Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China

Vernalization is a physiological process in which prolonged cold exposure establishes flowering competence in winter plants. In hexaploid wheat, TaVRN1 is a cold-induced key regulator that accelerates floral transition. However, the molecular mechanism underlying the gradual activation of TaVRN1 during the vernalization process remains unknown. In this study, we identified the novel transcript VAS (TaVRN1 alternative splicing) as a non-coding RNA derived from the sense strand of the TaVRN1 gene only in winter wheat, which regulates TaVRN1 transcription for flowering. VAS was induced during the early period of vernalization, and its overexpression promoted TaVRN1 expression to accelerate flowering in winter wheat. VAS physically associates with TaRF2b and facilitates docking of the TaRF2b-TaRF2a complex at the TaVRN1 promoter during the middle period of vernalization. TaRF2b recognizes the Sp1 motif within the TaVRN1 proximal promoter region, which is gradually exposed along with the disruption of a loop structure at the TaVRN1 locus during vernalization, to activate the transcription of TaVRN1. The tarf2b mutants exhibited delayed flowering, whereas transgenic wheat lines overexpressing TaRF2b showed earlier flowering. Taken together, our data reveal a distinct regulatory mechanism by which a long non-coding RNA facilitates the transcription factor targeting to regulate wheat flowering, providing novel insights into the vernalization process and a potential target for wheat genetic improvement.

关键词： winter wheat vernalization flowering long non-coding RNA chromosome loop

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OsCIPK7 point-mutation leads to conformation and kinase-activity change for sensing cold response

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Journal of Integrative Plant Biology 2019年第12期61卷 1194-1200页

作者： Dajian Zhang Xiaoyu Guo yunyuan xu Hao Li Liang Ma xuefeng Yao Yuxiang Weng Yan Guo Chun-Ming Liu Kang Chong Key Laboratory of Plant Molecular Physiology Institute of Botanythe Chinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Beijing 100049China Laboratory of Soft Matter Physics Institute of Physicsthe Chinese Academy of SciencesBeijing 100190China State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China

Calcineurin B-like interacting protein kinases(CIPKs) play important roles via environmental stress.However, less is known how to sense the stress in molecular structure conformation level. Here, an Os CIPK7 mutant via TILLING procedure with a point mutation in the kinase domain showed increased chilling tolerance, which could be potentially used in the molecular breeding. We found that this point mutation of Os CIPK7 led to a conformationalchange in the activation loop of the kinase domain,subsequently with an increase of protein kinase activity,thus conferred an increased tolerance to chilling stress.

关键词： conformation conformational activation

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The RING E3 ligase CLG1 targets GS3 for degradation via the endosome pathway to determine grain size in rice

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Molecular Plant 2021年第10期14卷 1699-1713页

作者： Wensi Yang Kun Wu Bo Wang Huanhuan Liu Siyi Guo Xiaoyu Guo Wei Luo Shengyuan Sun Yidan Ouyang Xiangdong Fu Kang Chong Qifa Zhang yunyuan xu Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhan 430070China National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research(Wuhan) Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China University of Chinese Academy of Sciences Beijing 100049China The State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Innovation Academy for Seed Design CASBeijing 100101China

G-protein signaling and ubiquitin-dependent degradation are both involved in grain development in rice,but how these pathways are coordinated in regulating this process is unknown.Here,we show that Chang Li Geng 1(CLG1),which encodes an E3 ligase,regulates grain size by targeting the Gγprotein GS3,a negative regulator of grain length,for degradation.Overexpression of CLG1 led to increased grain length,while overexpression of mutated CLG1 with changes in three conserved amino acids decreased grain length.We found that CLG1 physically interacts with and ubiquitinats GS3which is subsequently degraded through the endosome degradation pathway,leading to increased grain size.Furthermore,we identified a critical SNP in the exon 3 of CLG1 that is significantly associated with grain size variation in a core collection of cultivated rice.This SNP results in an amino acid substitution from Arg to Ser at position 163 of CLG1 that enhances the E3 ligase activity of CLG1 and thus increases rice grain size.Both the expression level of CLG1 and the SNP CLG1163S may be useful variations for manipulating grain size in rice.

关键词： grain size CLG1 GS3 E3 ligase endosome

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风环境视角下校园道路群落对PM_(10)的滞尘效应及优化研究

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城市建筑 2024年第9期21卷 218-223页

作者：徐阳洋李运远胡楠北京林业大学园林学院

以提升秋季校园道路群落滞尘效应为目标,探索不同室外风环境与群落特征对植物滞尘能力的影响,其次采用实测与PHOENICS模拟相结合的方式,分析并评价校园室外风环境,并使用线性回归验证了实验可行性,以此为校园绿地植物群落滞尘能力的提... 详细信息

以提升秋季校园道路群落滞尘效应为目标,探索不同室外风环境与群落特征对植物滞尘能力的影响,其次采用实测与PHOENICS模拟相结合的方式,分析并评价校园室外风环境,并使用线性回归验证了实验可行性,以此为校园绿地植物群落滞尘能力的提升提供建议。以北京林业大学校园内16条结构性道路为基础进行研究,结果显示:①秋季风速对道路群落滞尘效应的影响较为显著,实测数据中道路的平均风速值为0.44时群落滞尘能力达到最强,过高或过低的风速值均会降低植物的滞尘能力;②不同结构道路群落对PM_(10)滞尘效应的强弱顺序为:乔草型>乔灌型>乔灌草型>灌草型>乔木型,且滞尘能力差异较大,可达50%;③校园室外风环境中不同道路的风速差异较大,风速偏大的道路群落里普遍缺少低层次的灌木及小乔群落,应适当增添可防风的绿化。

关键词：道路群落 PM_(10) 滞尘效应风环境

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O-linked β-N-acetylglucosamine modification and its biological functions

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Science Bulletin 2015年第12期60卷 1055-1061,I0002页

作者： Yan Liu Shaojun Dai Lijing Xing yunyuan xu Kang Chong Alkali Soil Natural Environmental Science Center NortheastForestry University Key Laboratory of Saline-Alkali VegetationEcology Restoration in Oil Field Ministry of Education Harbin150040 China Key Laboratory of Plant Molecular Physiology Institute ofBotany Chinese Academy of Sciences Beijing 100093 China

到蛋白质的 Ser/Thr 残余的连接 O 的 -N-acetylglucosamine (O-GlcNAc ) 的共有原子价附件在原子核和细胞质充当不仅 posttranslational 修正而且一个营养的传感器，它直接调整基因和多重关键信号 transduction 小径的表示。在 Ser/Thr... 详细信息

到蛋白质的 Ser/Thr 残余的连接 O 的 -N-acetylglucosamine (O-GlcNAc ) 的共有原子价附件在原子核和细胞质充当不仅 posttranslational 修正而且一个营养的传感器，它直接调整基因和多重关键信号 transduction 小径的表示。在 Ser/Thr 残余的动态 O-GlcNAcylation 被二关键酶， O-GlcNAc transferase (OGT ) 和 O-GlcNAcase 催化，它分别地为 O-GlcNAc 修正的增加和移动负责。O-GlcNAc 修正在在动物的细胞的发信号起重要作用，特别在人的疾病。在植物的 OGT 的二 orthologs，敌探并且细长纤弱，被报导了涉及多样的植物过程。然而与在动物揭示的功能的机制相比，蛋白质 O-GlcNAc 的后果在植物的修正大部分是未知的，并且在 O-GlcNAcylation 之间的关系和细胞的过程需要被探索。在这评论，我们在动物在 O-GlcNAc 修正和它的生物功能上总结了最近的进展， O-GlcNAc 的更特殊的功能的植物，和前景将在植物被揭示。

关键词：生物学功能乙酰氨基葡糖胺翻译后修饰改性信号转导通路细胞信号转导营养传感器

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Structural repurposing of SGLT2 inhibitor empagliflozin for strengthening anti-heart failure activity with lower glycosuria

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Acta Pharmaceutica Sinica B 2023年第4期13卷 1671-1685页

作者： Yixiang xu Chao Zhang Kai Jiang Xinchun Yang Feng Chen Zhiyang Cheng Jinlong Zhao Jiaxing Cheng Xiaokang Li Xin Chen Luoyifan Zhou Hao Duan yunyuan Huang Yaozu Xiang Jian Li State Key Laboratory of Bioreactor Engineering Shanghai Frontiers Science Center of Optogenetic Techniques for Cell MetabolismFrontiers Science Center for Materiobiology and Dynamic ChemistryShanghai Key Laboratory of New Drug DesignSchool of PharmacyEast China University of Science and TechnologyShanghai 200237China Shanghai East Hospital School of Life Sciences and TechnologyTongji UniversityShanghai 200092China East China University of Science and Technology-Tengbai Pharmaceutical Innovative Drugs Joint Research Institute Zhuhai Tengbai Pharmaceutical Co.Ltd.Zhuhai 519000China Hubei Key Laboratory of Genetic Regulation and Integrative Biology School of Life SciencesCentral China Normal UniversityWuhan 430079China Yunnan Key Laboratory of Screening and Research on Anti-pathogenic Plant Resources from West Yunnan College of PharmacyDali UniversityDali 671000China Clinical Medicine Scientific and Technical Innovation Center Shanghai Tenth People's HospitalTongji University School of MedicineShanghai 200092China Key Laboratory of Tropical Biological Resources of Ministry of Education College of PharmacyHainan UniversityHaikou 570228China

Sodium-glucose cotransporter 2(SGLT2)inhibitors have been reapproved for heart failure(HF)therapy in patients with and without diabetes.However,the initial glucose-lowering indication of SGLT2i has impeded their uses in cardiovascular clinical practice.A challenge of SGLT2i then becomes how to separate their anti-HF activity from glucose-lowering side-effect.To address this issue,we conducted structural repurposing of EMPA,a representative SGLT2 inhibitor,to strengthen anti-HF activity and reduce the SGLT2-inhibitory activity according to structural basis of inhibition of SGLT2.Compared to EMPA,the optimal derivative JX01,which was produced by methylation of C2—OH of the glucose ring,exhibited weaker SGLT2-inhibitory activity(IC_(50)>100 nmol/L),and lower glycosuria and glucose-lowering side-effect,better NHE1-inhibitory activity and cardioprotective effect in HF mice.Furthermore,JX01 showed good safety profiles in respect of single-dose/repeat-dose toxicity and hERG activity,and good pharmacokinetic properties in both mouse and rat species.Collectively,the present study provided a paradigm of drug repurposing to discover novel anti-HF drugs,and indirectly demonstrated that SGLT2-independent molecular mechanisms play an important role in cardioprotective effects of SGLT2 inhibitors.

关键词： Heart failure SGLT2 inhibitor Empagliflozin Structural repurposing NHE1(sodium-hydrogen exchanger 1)

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Specific tracking of monoamine oxidase A in heart failure models by a far-red fluorescent probe with an ultra large Stokes shift

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Chinese Chemical Letters 2022年第3期33卷 1572-1576页

作者： Xinming Li Donglei Shi Yihe Song Yixiang xu Ying Gao Wenjing Qiu Xin Chen Xiaokang Li yunyuan Huang Yanjun Feng Baoli Li Yuan Guo Jian Li State Key Laboratory of Bioreactor Engineering Shanghai Key Laboratory of New Drug DesignSchool of PharmacyEast China University of Science and TechnologyShanghai 200237China Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education College of Chemistry and Materials ScienceNorthwest UniversityXi'an 710127China Frontiers Science Center for Materiobiology and Dynamic Chemistry East China University of Science and TechnologyShanghai 200237China College of Pharmacy and Chemistry Dali UniversityDali 671000China Clinical Medicine Scientific and Technical Innovation Center Shanghai Tenth People's HospitalTongji University School of MedicineShanghai 200092China

Monoamine oxidase A(MAO-A) is a prominent myocardial source of reactive oxygen species(ROS), and its expression and activity are strongly increased in failing hearts. Therefore, accurate evaluation of MAOA activity in cardiomyocytes is of great importance for understanding its biological functions and early diagnosing the progression of heart failure. However, so far, there is no report on the fluorescent diagnosis of heart failure by a specific probe for MAO-A. In this work, two far-red emissive fluorescent turn-on probes(KXS-M1 and KXS-M2) for the highly selective and sensitive detection of MAO-A were fabricated.Both probes exhibit good response to MAO-A, one of which, KXS-M2, performs better than the other one in terms of a fluorescence increment and sensitivity. Using the pioneering probe KXS-M2, specific fluorescence imaging of MAO-A in glucose-deprived H9c2 cardiac cells, zebrafish and isoprenaline-induced failing heart tissues was achieved, proving that KXS-M2 can serve as a powerful tool for the diagnosis and treatment of heart failure.

关键词： Heart failure Monoamine oxidase A Oxidative stress Fluorescent probe Far-red emission Diagnosis

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北京市海淀区典型植物群落内PM10浓度变化

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中国城市林业 2022年第1期20卷 21-28页

作者：许冬胡楠李运远北京林业大学园林学院北京100083

探究北京西、北环山因素影响下的相同结构植物群落PM_(10)浓度差异,可从城市视角为公园植物群落搭配及提升设计提供参考。本研究以北京市海淀区为例,测定不同山体位置关系下5个公园内20个典型植物群落PM_(10)浓度,分析不同植物群落内PM_... 详细信息

探究北京西、北环山因素影响下的相同结构植物群落PM_(10)浓度差异,可从城市视角为公园植物群落搭配及提升设计提供参考。本研究以北京市海淀区为例,测定不同山体位置关系下5个公园内20个典型植物群落PM_(10)浓度,分析不同植物群落内PM_(10)浓度时空变化、植物群落结构对PM_(10)的消减作用、PM_(10)浓度与温度及空气相对湿度的关系。结果表明:1)山体距离因子与PM_(10)浓度空间分布具有一定正相关关系,植物群落PM_(10)浓度日变化趋势呈现出"U型"的分布状态;2)植物群落结构PM_(10)消减率为草坪型>乔灌草型>乔草型>乔灌型;3)PM_(10)浓度与温度呈负相关关系,与空气相对湿度呈正相关关系,且温湿度对与山体距离远的公园影响更大。

关键词：城市绿地植物群落配置模式颗粒物北京海淀区

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