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Development of a C3c-based ELISA method for the determination of anti-complementary potency of Bupleurum polysaccharides

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Acta Pharmaceutica Sinica B 2015年第4期5卷 316-322页

作者： Mulu Wu Hong Li Yunyi Zhang Daofeng Chen Department of Pharmacology School of Pharmacy Fudan University Department of Microbiology Yong Loo Lin School of Medicine National University of Singapore Department of Pharmacognosy School of Pharmacy Fudan University

Traditionally, determination of inhibitory potency of complement inhibitors is performed by the hemolytic assay. However, this assay is not applicable to the lectin pathway, thus impeding the understanding of complement inhibitors against the overall function of the complement system. The main objective of our study was to develop a specific enzyme-linked immunosorbent assay(ELISA) as an alternative method to assess the anti-complement activity, particularly against the lectin pathway. By using respective coating substrates against different activation pathways, followed by capturing the stable C3c fragments, our ELISA method can be used to screen complement inhibitors against the classical pathway and the lectin pathway. The inhibitory effect of suramin on the classical pathway, as measured by our hemolytic assay is consistent with previous reports. Further assessment of suramin and Bupleurum polysaccharides against the lectin pathway showed a good reproducibility of the method. Comparison of the lectin pathway IC50 between Bupleurum smithii var. parvifolium polysaccharides(1.055 mg/mL) and Bupleurum chinense polysaccharides(0.98 mg/mL) showed that, similar to the classical and alterative pathway, these two Bupleurum polysaccharides had comparable anti-complementary properties against the lectin pathway. The results demonstrate that the described ELISA assay can compensate for the shortcomings of the hemolytic assay in lectin pathway.

关键词： ELISA Complement C3c Suramin Bupleurum smithii var parvifolium Bupleurum chinense Polysaccharides

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页岩油水平井穿层压裂先导性试验——以鄂尔多斯盆地庆城油田华H100平台为例

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中国石油勘探 2023年第4期28卷 92-104页

作者：张云逸中国石油集团川庆钻探工程有限公司长庆井下技术作业公司

鄂尔多斯盆地庆城油田华H100平台密集布井31口水平井,选取该平台因钻遇非储层即出层而导致低钻遇率的4口水平井,首次开展穿层压裂现场试验,同时应用示踪剂分段产能测试技术,研究穿透泥岩沟通相邻砂岩潜力储层的工程可行性,以及压裂后裂... 详细信息

鄂尔多斯盆地庆城油田华H100平台密集布井31口水平井,选取该平台因钻遇非储层即出层而导致低钻遇率的4口水平井,首次开展穿层压裂现场试验,同时应用示踪剂分段产能测试技术,研究穿透泥岩沟通相邻砂岩潜力储层的工程可行性,以及压裂后裂缝形成渗流通道的有效性,并对穿层压裂段是否有产量贡献进行确定性评价。试验结果表明:非储层段中水平井井眼轨迹与砂岩储层距离小于3m、泥质含量小于40%即可开展穿层压裂;试验14段、成功12段,成功率为85.7%,证实穿层压裂具有可行性。根据示踪剂监测解释结果,穿层压裂段有持续的返排产水,表明焖井之后支撑形成了有效渗流通道;各穿层压裂段不同程度的产油量也表明穿层压裂缝沟通了砂岩储层,具有确定的产能贡献。与同平台钻遇率大于80%的4口邻井对比,穿层压裂井累计产油量处于中等偏上水平。华H100平台穿层压裂试验的成功,从工程实践角度证明了穿层压裂是低钻遇率水平井提高单井产量的有效手段。

关键词：页岩油水平井穿层压裂华H100平台庆城油田

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Divergence in reservation prices and its impact on housing market liquidity: A theoretical analysis

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Journal of Management Science and Engineering 2018年第3期3卷 141-157页

作者： Pu Gong Yunyi Zhang Huainan Zhao Department of Finance School of ManagementHuazhong University of Science and TechnologyWuhan 430074China School of Business and Economics Loughborough UniversityLoughborough LE113TUUK

In this paper,we build a two-period English auction model to study the relative movements between buyers'and sellers'reservation prices in the housing market.We show that changes in sellers'reservation prices are jointly determined by changes in buyers'reservation prices,probability of buyers offering a high or low price,and the arrival rate of buyers.When the divergence between the buyers'and sellers'reservation prices widens,the probability of sale increases in the upward market and decreases in the downward market,contributing to the increases or decreases in market liquidity.

关键词： Reservation Price Housing Market Liquidity English Auction

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From germline genome to highly fragmented somatic genome:genome-wide DNA rearrangement during the sexual process in ciliated protists

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Marine Life Science & Technology 2024年第1期6卷 31-49页

作者： Liping Lyu Xue zhang yunyi Gao Tengteng zhang Jinyu Fu Naomi A.Stover Feng Gao Key Laboratory of Evolution&Marine Biodiversity(Ministry of Education) and Institute of Evolution&Marine BiodiversityOcean University of ChinaQingdao 266003China Department of Biology Bradley UniversityPeoriaIL 61625USA Laoshan Laboratory Qingdao 266237China

Genomes are incredibly dynamic within diverse eukaryotes and programmed genome rearrangements(PGR)play important roles in generating genomic diversity.However,genomes and chromosomes in metazoans are usually large in size which prevents our understanding of the origin and evolution of PGR.To expand our knowledge of genomic diversity and the evolutionary origin of complex genome rearrangements,we focus on ciliated protists(ciliates).Ciliates are single-celled eukaryotes with highly fragmented somatic chromosomes and massively scrambled germline genomes.PGR in ciliates occurs extensively by removing massive amounts of repetitive and selfish DNA elements found in the silent germline genome dur-ing development of the somatic genome.We report the partial germline genomes of two spirotrich ciliate species,namely Strombidium cf.sulcatum and Halteria grandinella,along with the most compact and highly fragmented somatic genome for S.cf.sulcatum.We provide the first insights into the genome rearrangements of these two species and compare these features with those of other ciliates.Our analyses reveal:(1)DNA sequence loss through evolution and during PGR in S.cf.sulcatum has combined to produce the most compact and efficient nanochromosomes observed to date;(2)the compact,transcriptome-like somatic genome in both species results from extensive removal of a relatively large number of shorter germline-specific DNA sequences;(3)long chromosome breakage site motifs are duplicated and retained in the somatic genome,revealing a complex model of chromosome fragmentation in spirotrichs;(4)gene scrambling and alternative pro-cessing are found throughout the core spirotrichs,offering unique opportunities to increase genetic diversity and regulation in this group.

关键词： Alternative processing Ciliates Gene scrambling Genome rearrangement Germline genome Somatic genome

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Beneficial effects of Houttuynia cordata polysaccharides on “two-hit” acute lung injury and endotoxic fever in rats associated with anti-complementary activities

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Acta Pharmaceutica Sinica B 2018年第2期8卷 218-227页

作者： Yan Lu Yun Jiang Lijun Ling Yunyi Zhang Hong Li Daofeng Chen Department of Pharmacognosy School of Pharmacy Fudan University Department of Pharmacology School of Pharmacy Fudan University

Houttuynia cordata Thunb. is a traditional herb used for clearing heat and eliminating toxins,and has also been used for the treatment of severe acute respiratory syndrome(SARS). In vitro, the crude H. cordata polysaccharides(CHCP) exhibited potent anti-complementary activity through both the classical and alternative pathways by acting on components C3 and C4 of the complement system without interfering with the coagulation system. This study was to investigate the preventive effects of CHCP on acute lung injury(ALI) induced by hemorrhagic shock plus lipopolysaccharide(LPS) instillation(two-hit)and LPS-induced fever in rats. CHCP significantly attenuated pulmonary injury in the "two-hit" ALI model by reducing pulmonary edema and protein exudation in bronchoalveolar lavage fluid(BALF). In addition, it reduced the deposit of complement activation products in the lung and improved oxidantantioxidant imbalance. Moreover, CHCP administration inhibited fever in rats, reduced the number of leukocytes and restored serum complement levels. The inhibition on the inappropriate activation of complement system by CHCP may play an important role in its beneficial effects on inflammatory diseases. The anti-complementary polysaccharides are likely to be among the key substances for the heatclearing function of H. cordata.

关键词： Houttuynia cordata Polysaccharides Fever Acute lung injury Anti-complementary

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解读马克思主义同中华优秀传统文化的“高度契合性”

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思想理论教育 2023年第7期 11-18页

作者：张允熠上海师范大学马克思主义与中国文化研究中心上海200234

中国传统哲学中的宇宙观、天下观、社会观、道德观是相互联系、辩证统一的,共同构成了中华优秀传统文化的核心价值观。从中华优秀传统文化丰厚资源和马克思主义理论宝库出发深入解读两者的“彼此契合性”,这对理解两者“互相结合”是必... 详细信息

中国传统哲学中的宇宙观、天下观、社会观、道德观是相互联系、辩证统一的,共同构成了中华优秀传统文化的核心价值观。从中华优秀传统文化丰厚资源和马克思主义理论宝库出发深入解读两者的“彼此契合性”,这对理解两者“互相结合”是必要的,对于学习和领会习近平新时代中国特色社会主义思想的文化主体性也是必需的。正是由于马克思主义同中华优秀传统文化存在着一种“高度契合性”,作为逻辑前提,两者才有可能进入相互“结合”的逻辑进程,没有“彼此契合”,就没有“互相结合”,两者结合的结果必然地生成了一个有机统一的新的文化生命体,从而使马克思主义成为中国的,中华优秀传统文化成为现代的,经由“结合”而成就了中国式现代化的文化新形态。

关键词：马克思主义中华优秀传统文化宇宙观天下观社会观道德观

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A Status Graph Based Control Allocation Algorithm of Digital Micro-Thruster Array for Micro/Nano-Satellites Orbit Control Application

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Transactions of Nanjing University of Aeronautics and Astronautics 2019年第5期36卷 779-788页

作者： zhang Dandan zhang yunyi DONG Ke LI Haiwang WANG Shaoping School of Energy and Power Engineering Beihang UniversityBeijing 100191P.R.China School of Automation Science and Electrical Engineering Beihang UniversityBeijing 100191P.R.China

Digital micro-thruster arrays can be used for special missions of micro/nano-satellites with the requirements of high precision and small impulse.This paper presents a novel control allocation algorithm for the digital micro-thruster array,namely status graph based control allocation(SGBCA)algorithm,which aims at finding the optimal micro thrusters combination scheme to realize the sequential control synthesis for micro/nano-satellite during real-time orbit control tasks.A mathematical model is set up for the control allocation of this multivariate over-actuated system.Through dividing thrusters into disjoint segments by offline calculation and combining segments dynamically online to provide a sequence of the required impulse for the micro/nano-satellite,the time complexity of the control allocation algorithm decreases significantly.All levels of impulse can be generated by the digital micro thruster arrays and the service life of the arrays can be extended using the segment converting strategy proposed in this paper.The simulation indicates that the algorithm can satisfy the requirements of real-time orbit control for micro/nano-satellites.

关键词： control allocation digital micro-thruster array micro/nano-satellite orbit control

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海德格尔哲学中的“友爱”--一种共享式的共在

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现代哲学 2022年第4期 90-97页

作者：张云翼中山大学哲学系广州510275

本文源于一个疑惑:为何海德格尔如此重视亚里士多德的实践智慧,却又没有对《尼各马可伦理学》中的关键篇章“友爱”进行过专题性的解读?“友爱”能否为基础存在论中未得到完整阐释的“共在”提供思想资源?本文从对“共在”的性质和样式... 详细信息

本文源于一个疑惑:为何海德格尔如此重视亚里士多德的实践智慧,却又没有对《尼各马可伦理学》中的关键篇章“友爱”进行过专题性的解读?“友爱”能否为基础存在论中未得到完整阐释的“共在”提供思想资源?本文从对“共在”的性质和样式的梳理出发、结合亚氏《尼各马可伦理学》中“友爱”,提出假设:友爱之“分享”正是共在问题结症之所在;然后,基于海氏笔下“此在之存在”与“善”的同一性,澄清“话语”的各个结构要素之功能(尤其是“共享”特性),以及基础存在论中的“共在”疑难;最后,笔者将跳出基础存在论,并试图通过深度无聊来构建一个与友爱之“分享”类似的、诸存在者共同存在(共享其存在)的可能图景。

关键词：共在友爱共享深度无聊自身存在

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情绪何以是一种变动?——从海德格尔对亚里士多德哲学概念πáθοs之阐释出发

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安徽大学学报（哲学社会科学版） 2022年第2期46卷 13-21页

作者：张云翼中山大学哲学系广东广州510275

海德格尔对情绪的处理非常独特。他没有将情绪仅仅视为关于此在之生存活动的后继情感反应,而是将其理解为一种构建着“此在之存在”的特殊变动。不过,这种颇具个人哲学特色的解读方式并非海德格尔所独创,而是在西方哲学史中有其根源。由... 详细信息

海德格尔对情绪的处理非常独特。他没有将情绪仅仅视为关于此在之生存活动的后继情感反应,而是将其理解为一种构建着“此在之存在”的特殊变动。不过,这种颇具个人哲学特色的解读方式并非海德格尔所独创,而是在西方哲学史中有其根源。由此,澄清情绪概念的思想来源,是理解情绪基本性质的关键。在《亚里士多德哲学的基本概念》中,海德格尔对πáθοs的解读就是一次对情绪概念的溯源考察。他的系统性阐释不但为理解情绪的变动性提供了理论支持,而且有助于厘清这一变动对“此在之存在”之构建的独特完善性作用。通过πáθοs,海德格尔还将亚里士多德哲学的核心概念(善、灵魂等)与“此在之存在”联系起来。这一联系提供了一个理解和评判他对亚里士多德哲学传统之继承、批判和发展的新的契机。

关键词：海德格尔情绪 πáθοs 采取姿态变动

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Effects of salidroside on glioma formation and growth inhibition together with improvement of tumor microenvironment

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Chinese Journal of Cancer Research 2013年第5期25卷 520-526页

作者： Yanshan zhang yunyi Yao Huijuan Wang Yanqin Guo Hui zhang Liang Chen Department of Gastric Surgery Wuwei Tumor Hospital Jiangsu Key Laboratory of Brain Disease Bioinfomation Research Center for Biochemistry and Molecular BiologyXuzhou Medical College Department of Tumor Chemotherapy Wuwei Tumor Hospital Department of Neurology Hongqi HospitalMudanjiang Medical University Department of Endocrinology 97th Hospital of Chinese PLA Department of Respiratory Medicine The Second Affiliated Hospital of Dalian Medical University

Objective：To test the effects of salidroside on formation and growth of glioma together with tumor microenvironment.Methods：Salidroside extracted from Rhodiola rosea was purified and treated on human glioma cells U251 at the concentration of 20 μg/mL.3-（4,5-dimethylthiazol-2-yl）-2,5-dephenyltetrazolium bromide （MTT） assay for cytotoxicity and flow cytometry （FCM） for cell cycle analysis were performed.Then for in vivo study,xenotransplantation tumor model in nude mice was generated and treated with salidroside at the concentration of 50 mg/kg.d for totally 20 d.Body weight and tumor size were detected every 2 d after the treatment.The levels of 8-isoprostane,superoxide dismutase （SOD） and malondialdehyde （MDA）,special markers for oxidative stress,were detected while immunofluoresence staining was performed for astrocyte detection.Results：For in vitro study,salidroside could decrease the viability of human glioma cells U251 and the growth of U251 cells at G0/G1 checkpoint during the cell cycle.For in vivo study,salidroside could also inhibit the growth of human glioma tissue in nude mice.The body weight of these nude mice treated with salidroside did not decrease as quickly as control group.In the tumor xenotransplantation nude mice model,mice were found of inhibition of oxidative stress by detection of biomarkers.Furthermore,overgrowth of astrocytes due to the stimulation of oxidative stress in the cortex of brain was inhibited after the treatment of salidroside.Conclusions：Salidroside could inhibit the formation and growth of glioma both in vivo and in vitro and improve the tumor microenvironment via inhibition of oxidative stress and astrocytes.

关键词： Glioma salidroside cytotoxicity cell cycle xenotransplantation oxidative stress astrocyte tumor microenvironment

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