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Microbiologically influenced corrosion resistance enhancement of coppercontaining high entropy alloy Fe_(x)Cu_(1−x)CoNiCrMn against Pseudomonas aeruginosa

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International Journal of Minerals,Metallurgy and Materials 2024年第11期31卷 2488-2497页

作者： yuntian lou Weiwei Chang Yu Zhang Shengyu He Xudong Chen Hongchang Qian Dawei Zhang Beijing Advanced Innovation Center for Materials Genome Engineering Institute for Advanced Materials and TechnologyUniversity of Science and Technology BeijingBeijing 100083China National Materials Corrosion and Protection Data Center University of Science and Technology BeijingBeijing 100083China BRI Southeast Asia Network for Corrosion and Protection(MOE) Shunde Innovation SchoolUniversity of Science and Technology BeijingFoshan 528399China School of Materials Science and Energy Foshan UniversityFoshan 528000China

To enhance the microbiologically influenced corrosion(MIC)resistance of FeCoNiCrMn high entropy alloy(HEAs),a series of Fe_(x)Cu_((1−x))CoNiCrMn(x=1,0.75,0.5,and 0.25)HEAs were *** characteristics,corrosion behavior(morphology observation and electrochemical properties),and antimicrobial performance of Fe_(x)Cu_((1−x))CoNiCrMn HEAs were evaluated in a medium inoculated with typical corrosive microorganism Pseudomonas *** aim was to identify copper-containing FeCoNiCrMn HEAs that balance corrosion resistance and antimicrobial *** revealed that all Fe_(x)Cu_((1−x))CoNiCrMn(x=1,0.75,0.5,and 0.25)HEAs exhibited an FCC(face centered cubic)phase,with significant grain refinement observed in Fe_(0.75)Cu_(0.25)CoNiCrMn *** tests indicated that Fe_(0.75)Cu_(0.25)CoNiCrMn HEA demonstrated lower corrosion current density(i_(corr))and pitting potential(E_(pit))compared to other Fe_(x)Cu_((1−x))CoNiCrMn HEAs in ***-inoculated medium,exhibiting superior resistance to ***-microbial tests showed that after 14 d of immersion,Fe_(0.75)Cu_(0.25)CoNiCrMn achieved an antibacterial rate of 89.5%,effectively inhibiting the adhesion and biofilm formation of ***,thereby achieving resistance to MIC.

关键词： microbiologically influenced corrosion Pseudomonas aeruginosa high entropy alloys antibacterial property

Microbiologically influenced corrosion of 304 stainless steel by halophilic archaea Natronorubrum tibetense

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Journal of Materials Science & Technology 2020年第11期42卷 12-20页

作者： Hongchang Qian Lingwei Ma Dawei Zhang Ziyu Li Luyao Huang yuntian lou Cuiwei Du Beijing Advanced Innovation Center for Materials Genome Engineering Institute for Advanced Materials and TechnologyUniversity of Science and Technology BeijingBeijing100083China

The corrosion behavior of 304 stainless steel(SS)in the presence of aerobic halophilic archaea Natronorubrum tibetense was *** 14 days of immersion,no obvious pitting pit was observed on the SS surface in the sterile *** contrast,the SS exhibited serious pitting corrosion with the largest pit depth of 5.0μm in the inoculated medium after 14 *** results of electrochemical tests showed that the barrier property of the passive film decreased faster in the inoculated *** X-ray photoelectron spectroscopy results indicated that the detrimental Fe2+and Cr6+increased in the passive film under the influence of archaea ***,which resulted in the accelerated deterioration of passive film and promoted the pitting *** with the energy starvation tests,the microbiologically influenced corrosion mechanism of 304 SS caused by halophilic archaea *** was discussed finally.

关键词： Stainless Steel Archaea Microbiological influenced corrosion

Microbiologically Influenced Corrosion Behavior of Fe_(40)(CoCrMnNi)_(60) and Fe_(60)(CoCrMnNi)_(40) Medium Entropy Alloys in the Presence of Pseudomonas Aeruginosa

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Acta Metallurgica Sinica(English Letters) 2023年第3期36卷 379-390页

作者： Weiwei Chang Yangyang Li Huaibei Zheng Hongchang Qian Dawei Guo Shuyuan Zhang yuntian lou Chi Tat Kwok Lap Mou Tam Dawei Zhang National Materials Corrosion and Protection Data Center Institute for Advanced Materials and TechnologyUniversity of Science and Technology BeijingBeijing 100083China BRI Southeast Asia Network for Corrosion and Protection(MOE) Shunde Graduate School of University of Science and Technology BeijingFoshan 528399China State Key Laboratory of Metal Material for Marine Equipment and Application Anshan 114002China Department of Electromechanical Engineering University of MacaoMacao 999078China Institute for the Development and Quality Macao 999078China

In this work,the microbiologically influenced corrosion(MIC)of Fe_(40)(CoCrMnNi)_(60) and Fe_(60)(CoCrMnNi)_(40) medium entropy alloys(MEAs)induced by Pseudomonas aeruginosa(***)was *** behaviors during 14 days of immersion in sterile and ***-inoculated culture media are *** sterile conditions,both MEAs exhibited good corrosion resistance against the culture medium *** the presence of ***,the pitting corrosion of MEAs was *** results of inductively coupled plasma‒mass spectrometry(ICP‒MS)and potentiodynamic polarization tests showed that the presence of *** promoted the selective dissolution of passive film and accelerated the corrosion of *** results of X-ray photoelectron spectroscopy(XPS)and Mott-Schottky measurements further demonstrated the degradation effect of *** on the passive *** with Fe_(60)(CoCrMnNi)_(40),Fe_(40)(CoCrMnNi)_(60) manifested better resistance to the MIC caused by ***,which may be attributed to more Cr oxides and fewer Fe oxides of the passive film.

关键词： Microbiologically influenced corrosion Medium entropy alloys Pseudomonas aeruginosa

Methanogenic archaea and sulfate reducing bacteria induce severe corrosion of steel pipelines after hydrostatic testing

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Journal of Materials Science & Technology 2020年第13期47卷 72-83页

作者： Enze Zhou Jianjun Wang Masoumeh Moradi Huabing Li Dake Xu yuntian lou Jinheng Luo Lifeng Li Yulei Wang Zhenguo Yang Fuhui Wang Jessica A.Smith Shenyang National Laboratory for Materials Science Northeastern UniversityShenyang 110819China Key Laboratory for Anisotrotropy and Texture of Materials(Ministry of Education) School of Materials Science and EngineeringNortheastern UniversityShenyang 110819China Corrosion and Protection Center University of Science and Technology BeijingBeijing 100083China CNPC Tubular Goods Research Institute Xian 710065China CNPC Pipeline Construction Administration Department Beijing 100101China Institute of Metal Research Chinese Academy of SciencesShenyang 110016China Department of Biomolecular Sciences Central Connecticut State UniversityNew BritainCT 06050USA

Complex interactions within a microbial consortium can induce severe corrosion in oil *** study investigated the mechanism of microbiologically influenced corrosion(MIC)that led to failure of X52 steel pipelines after hydrostatic *** hydrostatic testing with untreated lake water and underground water were used to simulate and study the events that led to the actual *** analysis,weight loss,and several electrochemical measurements demonstrated rapid corrosion rates after hydrostatic *** of microbial community structures revealed that methanogenic archaea and sulfate reducing bacteria(SRB),introduced by the hydrotest water,formed corrosive biofilms on X52 steel coupon surfaces that induced severe pitting.

关键词： Pipeline steel Microbiologically influenced corrosion Sulfate-reducing bacteria(SRB) Pitting corrosion

杀菌剂THPS对X80管线钢应力腐蚀开裂影响及机理研究

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表面技术 2024年第20期53卷 69-81页

作者：杨吉可李众王军磊娄云天李晓刚北京科技大学新材料技术研究院腐蚀与防护中心北京100083 国家材料腐蚀与防护科学数据中心北京100083 军事科学院国防工程研究院河南洛阳471000

目的研究杀菌剂THPS对X80管线钢应力腐蚀开裂的影响及机理。方法 37℃下,将X80钢U弯和10 mm×10 mm平片浸泡在接种了硫酸弧菌(Desulfovibrio vulgaris)的150 mL ATCC 1249培养基(添加杀菌剂THPS质量浓度为0、15、30、45、60 mg/L)中培... 详细信息

目的研究杀菌剂THPS对X80管线钢应力腐蚀开裂的影响及机理。方法 37℃下,将X80钢U弯和10 mm×10 mm平片浸泡在接种了硫酸弧菌(Desulfovibrio vulgaris)的150 mL ATCC 1249培养基(添加杀菌剂THPS质量浓度为0、15、30、45、60 mg/L)中培养14 d。通过细胞计数实验、失重测试、H2S和H2测量、扫描电子显微镜(SEM)测试、激光共聚焦荧光显微镜(CLSM)测试、开路电位(OCP)、电化学阻抗(EIS)、线性极化电阻(LPR)、动电位极化测试、热力学计算和光电子能谱仪XPS,分别对X80钢在不同浓度杀菌剂含量的***中的腐蚀活动、应力腐蚀开裂裂纹生长情况、杀菌剂对生物膜的影响以及腐蚀产物进行分析。结果失重、固着细胞计数和浮游细胞计数3项数据均呈现随着THPS浓度升高而下降的趋势。同时,SEM也可以观察到X80钢U弯在不同浓度下的THPS中形成的裂纹长度随着THPS的浓度增大呈减小趋势。OCP、EIS、LPR和动电位极化结果证实了杀菌剂THPS浓度增加导致微生物腐蚀活动下降,而较慢的微生物腐蚀活动减缓了X80钢的应力腐蚀开裂。通过理论计算确定了THPS对X80钢U弯的吸附类型。结论杀菌剂THPS对SRB具有有效杀灭和驱散作用,从而减缓了X80钢的应力腐蚀开裂,THPS对X80钢U弯的吸附类型为静电导致的物理吸附和电荷交换导致的化学吸附二者兼有。

关键词：微生物腐蚀硫酸盐还原菌应力腐蚀开裂杀菌剂

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考虑全功率换流器低电压穿越的潮流解存在性分析

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浙江电力 2023年第2期42卷 17-25页

作者：兰洲但扬清谷纪亭娄云天国网浙江省电力有限公司经济技术研究院杭州310008 浙江大学电气工程学院杭州310027

针对低电压穿越期间含全功率换流器系统可能失去潮流解的问题,分析了故障期间电网等值参数和潮流特性的变化规律,考虑换流器的控制特性,采用数值计算定性分析了不同系统变量对潮流解存在性的影响规律。基于电网等值参数变化规律和潮流... 详细信息

针对低电压穿越期间含全功率换流器系统可能失去潮流解的问题,分析了故障期间电网等值参数和潮流特性的变化规律,考虑换流器的控制特性,采用数值计算定性分析了不同系统变量对潮流解存在性的影响规律。基于电网等值参数变化规律和潮流雅克比矩阵行列式实时计算的方法,提出了仅需非故障电网等值参数和并网点实测电压的有功电流限幅自律控制方法。仿真结果表明,所提方法能够保证故障低电压穿越期间潮流解的存在,有利于电力系统的安全稳定运行。

关键词：新能源发电并网全功率换流器低电压穿越潮流解存在性自律控制

草莓FaUVR8基因的cDNA克隆、生物信息学分析及原核表达条件优化

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分子植物育种 2016年第11期14卷 2964-2975页

作者：刘勇强王熙然叶云天娄可飞陈清汤浩茹四川农业大学园艺学院成都611130

紫外线B(UV-B)对于植物生长发育具有重要调节作用,其中UV RESISTANCE LOCUS 8蛋白作为UV-B的光受体在植物响应UV-B过程中起关键作用。草莓是一种重要的经济果树,目前关于草莓的UV-B光受体报道较少。为研究草莓中UV-B受体蛋白,本研究采... 详细信息

紫外线B(UV-B)对于植物生长发育具有重要调节作用,其中UV RESISTANCE LOCUS 8蛋白作为UV-B的光受体在植物响应UV-B过程中起关键作用。草莓是一种重要的经济果树,目前关于草莓的UV-B光受体报道较少。为研究草莓中UV-B受体蛋白,本研究采用同源克隆的方法获得‘丰香’草莓中编码UV-B光受体蛋白的c DNA序列,进行了生物信息学的分析并构建了原核生物表达载体p ET32a-UVR8,利用中心点响应面法优化了重组蛋白的诱导条件(包括诱导温度,IPTG浓度,菌液浓度和诱导时间)。结果表明:克隆得到了编码草莓UVR8蛋白的全长c DNA序列,命名为Fa UVR8,生物信息学分析表明该序列长1 317 bp,编码438个氨基酸,预测分子量为47.28 k D,等电点p I为5.85,重建蛋白质三维结构表明该蛋白包含7个β-片状螺旋结构;原核表达得到69.7 k D的融合蛋白,对四个诱导条件优化表明将工程菌培养至菌液OD600=0.7,加入浓度为0.55 mmol/L的IPTG,在27.72℃下诱导9 h,可获得最大量重组蛋白79μg/m L。研究结果可为后期寻找与Fa UVR8蛋白互作的下游蛋白,以及进一步开展其功能研究提供参考。

关键词：草莓 FaUVR8 生物信息学原核表达

森林草莓B-box基因家族全基因组序列鉴定与表达分析

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分子植物育种 2016年第4期14卷 827-834页

作者：叶云天娄可飞王熙然刘勇强陈清汤浩茹四川农业大学园艺学院成都611130

植物B-box基因参与植物形态建成、花器官发育以及响应逆境胁迫过程中的多种生命活动的并具有重要作用,草莓是重要的经济果树作物,然而对草莓B-box家族了解较少。为了研究草莓B-box基因家族成员及其在花器官发育过程中的表达情况,本研究... 详细信息

植物B-box基因参与植物形态建成、花器官发育以及响应逆境胁迫过程中的多种生命活动的并具有重要作用,草莓是重要的经济果树作物,然而对草莓B-box家族了解较少。为了研究草莓B-box基因家族成员及其在花器官发育过程中的表达情况,本研究利用计算生物学的方法从全基因组水平鉴定森林草莓(Fragaria vesca)B-box基因家族成员,并对家族成员的蛋白质特征、基因组分布、内含子个数以及基因表达进行分析。结果表明,森林草莓中至少存在21个B-box基因家族成员,分布在6条染色体上,基因内含子个数1~5个。基于蛋白质模体组成与蛋白质序列的分析将整个家族分为五个亚家族。14个基因家族成员以5种表达模式参与雄蕊器官发育进程。研究结果表明:草莓B-box基因家族的5个亚家族经过模体复制和删除事件进化而来,并参与花器官发育过程。试验结果将为在草莓中进一步研究该基因家族成员功能提供参考。

关键词：草莓基因家族生物信息学 B-box