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A High-Affinity Methyl-CpG-Binding Protein for Endonuclease-Free and Label-Free DNA Methyltransferase Activity Detection

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Journal of Analysis and Testing 2024年第3期8卷 327-334页

作者： Yang Bai Shulin Tan Yingsong Sheng Yueqing Gu Haiping Wu Baicun Li yunlong Liu Department of Biomedical Engineering School of Engineering China Pharmaceutical University Department of Clinical Pharmacy Jinling Hospital State Key Laboratory of Analytical Chemistry for Life Science & Jiangsu Key Laboratory of Molecular Medicine Medical School of Nanjing University School of Pharmaceutical Sciences Southern Medical University National Center for Respiratory Medicine Center of Respiratory Medicine China-Japan Friendship Hospital

DNA methyltransferase(MTase) activity detection has received increasing attention as a promising biomarker and therapeutic target. However, most of these detection methods rely on endonuclease digestion and signal groups ***, we present a novel platform for sensing DNA MTase activity that overcomes these limitations. Our approach is both endonuclease-free and label-free, utilizing a combination of a high-affinity streptavidin-methyl-CpG-binding domain(SA-MBD) protein and surface plasmon resonance(SPR) technology. The SA-MBD protein specifically recognizes a hairpin probe containing methylated CpG sites, which is treated with *** MTase. This recognition event generates a corresponding SPR response signal. The limit of detection is as low as 0.016 U/mL, owing to the high-a ffinity of the SA-MBD ***, we have demonstrated the feasibility of our method for *** MTase activity analysis in serum and inhibitor screening, which implies the potential prospects for biomedical research.

关键词： Biosensing DNA methyltransferase Inhibitor screening Methyl-CpG-binding protein Surface plasmon resonance

Progress on gas-solid phase photoreactor and its application in CO_(2) reduction

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Green Chemical Engineering 2024年第3期5卷 290-306页

作者： Litian Liu yunlong Li Jialun He Qing Wang Juan Deng Xiao Chen Chao Yu School of Environmental and Chemical Engineering Jiangsu University of Science and TechnologyZhenjiang212100China

The burgeoning field of photocatalytic reduction of CO_(2)has emerged as a remarkable promising solution to address some of the most pressing global energy and environmental issues which we face *** around the global have been striving to augment the efficiency of CO_(2)photocatalytic reduction,employing strategies that range from modifying the fundamental properties of photocatalysts to suppress the electron-hole recombination,optimizing reaction conditions to achieve the highest yield,and conceptualizing and constructing photoreactors to improve the adsorption *** these factors,the photoreactor plays a critical role in enhancing the overall photocatalytic *** the various types of photoreactors and their operational dynamic can significantly influence the experimental design,thus guiding the data collecting and *** to the solid-liquid phase,gas-solid phase photocatalytic reduction of CO_(2)is gaining recognition for its potential advantages,such as rapid molecular diffusion rates,adjustable CO_(2)concentrations,and uniform and sufficient light ***,the currently reported gas-solid phase photoreactors are still in their *** this review,we dissect the underlying mechanism of photocatalytic CO_(2)reduction and the performance evaluation criteria of photoreactors,and review the development process of gas-solid phase ***,we explore the evolution of gas-solid phase photoreactors,elucidating their growth trajectory and future *** present a comprehensive classification of gas-solid phase photoreactors,offering a new insight into their design and functionality,summarizing their strengths and inevitable ***,we provide a forward-looking perspective on the future developmental prospects of carbon neutrality.

关键词： Photoreactor Gas-solid phase Carbon dioxide reduction Photocatalysis

Electrohydrodynamic printing for high resolution patterning of flexible electronics toward industrial applications

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InfoMat 2024年第2期6卷 28-56页

作者： Zhouping Yin Dazhi Wang yunlong Guo Zhiyuan Zhao Liqiang Li Wei Chen Yongqing Duan State Key Laboratory of Intelligent Manufacturing Equipment and Technology Huazhong University of Science and TechnologyWuhanthe People's Republic of China Optics Valley Laboratory Wuhanthe People's Republic of China Key Laboratory for Micro/Nano Technology and System of Liaoning Province Dalian University of TechnologyDalianthe People's Republic of China Beijing National Laboratory for Molecular Sciences Key Laboratory of Organic SolidsInstitute of ChemistryChinese Academy of SciencesBeijingthe People's Republic of China Institute of Molecular Aggregation Sciences Tianjin UniversityTianjinthe People's Republic of China

Electrohydrodynamic(EHD)printing technique,which deposits micro/nanostructures through high electric force,has recently attracted significant research interest owing to their fascinating characteristics in high resolution(<1μm),wide material applicability(ink viscosity 1–10000 cps),tunable printing modes(electrospray,electrospinning,and EHD jet printing),and compatibility with flexible/wearable *** the laboratory level of the EHD printed electronics'resolution and efficiency is gradually approaching the commercial application level,an urgent need for developing EHD technique from laboratory into industrialization have been put ***,we first discuss the EHD printing technique,including the ink design,droplet formation,and key technologies for promoting printing efficiency/*** we summarize the recent progress of EHD printing in fabrication of displays,organic field-effect transistors(OFETs),transparent electrodes,and sensors and ***,a brief summary and the outlook for future research effort are presented.

关键词： display electrohydrodynamic printing flexible electronics organic field-effect transistor printhead

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威海市海洋生态文明示范区建设效果初探

绿色科技 2020年第2期22卷 253-256,259页

作者：庞云龙山东省威海市人才创新发展院山东威海264200

指出了建设海洋生态文明是生态文明思想在海洋发展领域的应用与实践。威海作为国家首批海洋生态文明示范城市,积极推动海洋经济绿色发展,坚持构建和谐人海关系,但威海市海洋生态文明示范区尚未建立科学的评估机制。分析了威海市建设海... 详细信息

指出了建设海洋生态文明是生态文明思想在海洋发展领域的应用与实践。威海作为国家首批海洋生态文明示范城市,积极推动海洋经济绿色发展,坚持构建和谐人海关系,但威海市海洋生态文明示范区尚未建立科学的评估机制。分析了威海市建设海洋生态文明示范区的优势及具体实践,初步评估子威海市海洋生态文明示范区建设情况。结果显示:威海市在海洋经济发展、海洋资源利用、海洋生态环境保护、保障体系方面成效显著,在海洋文化培育方面略显不足。进一步探究了威海市海洋生态文明建设存在的问题,提出了相应的建议:威海市调整优化评价指标体系、完善海洋经济统计工作。在此基础上,初步建立威海市海洋生态文明建设指标体系。

关键词：威海市海洋生态文明效果评估

Identification prognostic features related to sphingolipid metabolism and experimental validation of TRIM47 in hepatocellular carcinoma

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BIOCELL 2024年第4期48卷 639-651页

作者： JIAN TANG CHENQIANG ZHU YUN CHEN yunlong WU MING HE YI ZHOU MINGHUA XIE Department of Intensive Care Unit The First People’s Hospital of Linping DistrictHangzhouChina Department of Pharmacy The First People’s Hospital of Linping DistrictHangzhouChina

Background:The speciﬁc impact of sphingolipid metabolism on developing hepatocellular Carcinoma(HCC)remains *** study aims to explore the relationship between sphingolipid metabolism and HCC prognosis,immune response,and drug ***:Data were obtained from The Cancer Genome Atlas(TCGA)-Hepatocellular Carcinoma(LIHC)and Gene Expression Omnibus(GEO,GSE14520 datasets).47 sphingolipid metabolism genes were obtained from the Kyoto Encyclopedia of Genes and Genomes(KEGG)*** classifying HCC samples using the Non-negative Matrix Factorization(NMF)clustering method,differentially expressed genes were ***,8 risk genes were obtained by univariate analysis,survival random forest reduction and lasso *** expression of 8 risk genes was veriﬁed in ***:8 risk genes were used to construct the Sphingolipid score ***-Sphingolipid score predicted poor prognosis of HCC *** score was associated with immune checkpoints(IL-1B,TLR4,TGFB1,and IL-10),immune cells(Th2,Treg,MDSC,Neutrophil,Fibroblasts and macrophage),and MAPK *** the High-Sphingolipid score group,a signiﬁcantly higher proportion of patients with TP53(p53)mutations was signiﬁcantly higher(56%).Furthermore,patients with a high-Sphingolipid score were predicted to have a higher sensitivity to chemotherapy *** vitro validation showed that compared with normal liver cells LX-2,TRIM47,and S100A9 signiﬁcantly increased in liver cancer cells Hep G2,MHCC-97H,and Hep3B2.1-7,while SLC1A7,LPCAT1,and CFHR4 signiﬁcantly *** TRIM47 reduced the proliferation and promoted *** levels of ceramide synthesis-related indexes(CERS1,CERS6,CERS5,and SPTLC2)increased,and the ACER3 related to catalytic hydrolysis ***:We constructed a sphingolipid metabolism-related prognostic signature(Sphingolipid score)based on 8 risk ***47 may affect the development of liver cancer by regulating the relevant indicators of

关键词： Hepatocellular carcinoma Sphingolipid metabolism TRIM47 Prognosis

Identification and hazard analysis of landslides triggered by earthquakes and rainfall

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Earthquake Research Advances 2024年第3期4卷 42-54页

作者： Lei Li Yu Zhang yunlong Hou Bingbing Han Ning An Hui Zhang Ying Ma School of Traffic and Transportation Lanzhou Jiaotong UniversityLanzhou730070China Key Laboratory of Railway Industry on Plateau Railway Transportation Intelligent Management and Control Lanzhou Jiaotong UniversityLanzhou730070China Gansu Institute of Engineering Geology Lanzhou730030China School of Civil Engineering Lanzhou Jiaotong UniversityLanzhou730070China

This study aims to utilize the Small Baseline Subset Interferometric Synthetic Aperture Radar(SBAS-In SAR)technique and Google Earth optical remote sensing images to analyze the area within 20 km around the epicenter of a M 3.9, earthquake that occurred in Tanchang County, Gansu Province, on December 28, 2020. The objective is to identify potential earthquake-induced landslides, assess their scale, and determine their impact range. The study results reveal the successful identification of two potential landslides in the 20 km radius around the epicenter. Through time-series deformation analysis, it was observed that these potential landslides were significantly influenced by both the earthquake and rainfall. Further estimation of these potential landslides indicates maximum depths of 7.4 m and 14.1 m for the failure surfaces, with volumes of 9.02 × 10~4m~3and 25.5 ×10~4m~3, respectively. Finally, based on the simulation analysis of Massflow software, the maximum thickness of soil accumulation in the final accumulation area after sliding of the potential landslide in Shangyaai is 12 m, the area of the final accumulation area is 1.75 × 10~4m~2, and the farthest movement distance is 1124 m. The maximum thickness of soil accumulation in the final accumulation area after sliding of the potential landslide in Wangshancun is 8 m, the area of the final accumulation area is 7.89 × 10~4m~2, and the farthest movement distance is 742 m.

关键词： SBAS-InSAR technology Earthquake Deformation monitoring Landslide Massflow Hazard analysis

Capacity degradation analysis and knee point prediction for lithium-ion batteries

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Green Energy and Intelligent Transportation 2024年第5期3卷 13-22页

作者： Teng Wang Yuhao Zhu Wenyuan Zhao Yichang Gong Zhen Zhang Wei Gao yunlong Shang Shandong University Jinan250061China San Diego State University San DiegoUSA

Analyzing capacity degradation characteristics and accurately predicting the knee point of capacity are crucial for the safety management of lithium-ion batteries(LIBs).However,the degradation mechanism of LIBs is complex.A key but challenging problem is how to clarify the degradation mechanism and predict the knee *** to the external characteristics such as capacity decline gradievnt and the peak value of increment capacity curve(IC curve),the capacity degradation can be divided into four stages,including initial decline stage,slow decline stage,transition stage and high-speed decline *** degradation mechanism of LIBs is compared from the longitudinal and horizontal aspects,*** them,the battery usage from the initial stage to the end of life(EOL)is longitudinal *** battery under different conditions,such as charging and discharging,different discharge rate,different cathode material degradation mechanism is horizontal ***,a method based on neural network is proposed to predict the knee *** features are used to predict the capacity and cycle of the knee point,which are the gradient of the capacity degradation curve and the difference of the IC curve with the maximum *** experimental results show that a two-dimensional surface can be obtained using only the first 100 cycles,which can provide a reference for the position of the knee point accurately prediction.

关键词： Lithium-ion batteries Multistage capacity degradation Knee point prediction Neural network

Research on active defense decision-making method for cloud boundary networks based on reinforcement learning of intelligent agent

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High-Confidence Computing 2024年第2期4卷 50-61页

作者： Huan Wang yunlong Tang Yan Wang Ning Wei Junyi Deng Zhiyan Bin Weilong Li School of Computer Science and Technology Guangxi University of Science and TechnologyLiuzhou 545000China Liuzhou Key Laboratory of Big Data Intelligent Processing and Security Liuzhou 545000China Guangxi Education System Network Security Monitoring Center Liuzhou 545000China Center of Graphics and Information Guangxi ECO-Engineering Vocational&Technical CollegeLiuzhou 545004China

The cloud boundary network environment is characterized by a passive defense strategy,discrete defense actions,and delayed defense feedback in the face of network attacks,ignoring the influence of the external environment on defense decisions,thus resulting in poor defense ***,this paper proposes a cloud boundary network active defense model and decision method based on the reinforcement learning of intelligent agent,designs the network structure of the intelligent agent attack and defense game,and depicts the attack and defense game process of cloud boundary network;constructs the observation space and action space of reinforcement learning of intelligent agent in the non-complete information environment,and portrays the interaction process between intelligent agent and environment;establishes the reward mechanism based on the attack and defense gain,and encourage intelligent agents to learn more effective defense *** designed active defense decision intelligent agent based on deep reinforcement learning can solve the problems of border dynamics,interaction lag,and control dispersion in the defense decision process of cloud boundary networks,and improve the autonomy and continuity of defense decisions.

关键词： Active defense decision-making Cloud boundary network security Intelligent agent reinforcement learning Offensive and defensive game

Dissecting genetic loci affecting grain morphological traits to improve grain weight via nested association mapping

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Dissecting genetic loci affecting grain morphological traits...