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Allelic impacts on pre-harvest sprouting resistance and favorable haplotypes in Ta PHS1 of Chinese wheat accessions

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The Crop Journal 2020年第4期8卷 515-521页

作者： Danfeng Wang yunlong pang Lei Dong Anfei Li Lingrang Kong Shubing Liu State Key Laboratory of Crop Biology College of AgronomyShandong Agricultural UniversityTai'an 271018ShandongChina

Pre-harvest sprouting(PHS)influences yield and end-use quality of bread *** varieties with PHS resistance is the most effective way to reduce this *** this study,a panel of 725 Chinese wheat accessions were evaluated for PHS resistance in three *** was abundant variation in PHS resistance and 63 accessions showing high resistance had germination rates of less than 10%across three *** distribution of three causal single nucleotide polymorphisms in Ta PHS1 at bases-222,+646,and+666 were assessed and frequencies were *** alleles conferring PHS resistance were identified for each *** analysis showed that bases C,G,and A at each of the three loci comprised the best haplotype for PHS resistance,whereas TAT showed the highest sprouting *** with the superior Ta PHS1 haplotypes proved to be resistant to PHS providing a basis to develop varieties with PHS resistance through marker assisted breeding.

关键词： PHS resistance bases

Comparison of the photo-acclimation potential of fl oating and benthic thalli of Sargassum horneri(Phaeophyta)during autumn and winter

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Journal of Oceanology and Limnology 2022年第1期40卷 195-205页

作者： Jingjing LI yunlong pang Song QIN Zhengyi LIU Zhihai ZHONG Wanlin SONG Longchuan ZHUANG Key Laboratory of Marine Hazards Forecasting Ministry of Natural ResourcesHohai UniversityNanjing 210098China Yantai Institute of Coastal Zone Research Chinese Academy of SciencesYantai 264003China Weihai Vocational College Weihai 264200China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China

Sargassum horneri is a foundational species and an important contributor to the fl oating seaweed stock along the northeastern coast of *** this study,benthic and fl oating thalli of *** were collected from Changdao Island(37°54′N,120°43′E),Bohai Bay,*** conducted an in-situ and an indoor experiment to study the acclimation potential in *** to abiotic conditions at sea surface in autumn and *** benthic and fl oating thalli were cultured in situ for two months(from October to December)at diff erent depths:0 m above sea level(masl)and 3 m below sea level(mbsl),and their growth rate,biochemical content,and photosynthetic performance were *** the fi rst month of culture,the relative growth rate of fl oating thalli was 2-fold greater than that of benthic thalli at 0 *** photosynthetic rate of most thalli was signifi cantly higher at 0 masl than at 3 *** the indoor experiments,fl oating and benthic thalli were exposed to high light intensity(400μmol photons/(m^(2)·s)photosynthetically active radiation(PAR))for 21 d,and their photo-acclimation capacities were *** high light intensity,the two types of thalli showed low maximum quantum yield(F_(v)/F_(m))and light utilisation effi ciency(α)but high light saturation point(E_(k)).Floating thalli showed higher photosynthetic rate and photoprotective ability than benthic thalli at high light *** eff ective quantum yield of photosystem II[Y(II)]of both types of thalli recovered after a 6-day treatment with low light intensity(40μmol photons/(m^(2)·s)).These fi ndings suggest that *** is highly acclimated to the sea surface environment,which possibly contributes to its rapid accumulation and long free-fl oating periods at the sea surface.

关键词： chlorophyll-a fl uorescence growth photosynthesis photosynthetic pigments Sargassum horneri

Genetic bases of source-,sink-,and yield-related traits revealed by genome-wide association study in Xian rice

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The Crop Journal 2020年第1期8卷 119-131页

作者： Yun Wang yunlong pang Kai Chen Laiyuan Zhai Congcong Shen Shu Wang Jianlong Xu Rice Research Institute Shenyang Agricultural UniversityShenyang 110866LiaoningChina Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement Chinese Academy of Agricultural SciencesBeijing 100081China Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural SciencesShenzhen 518120GuangdongChina

The source-sink relationship determines the ultimate grain *** investigated the genetic basis of the relationship between source and sink and yield potential in *** two environments,we identified quantitative trait loci(QTL)associated with sink capacity(total spikelet number per panicle and thousand-grain weight),source leaf(flag leaf length,flag leaf width and flag leaf area),source-sink relationship(total spikelet number to flag leaf area ratio)and yield-related traits(filled grain number per panicle,panicle number per plant,grain yield per plant,biomass per plant,and harvest index)by genome-wide association analysis using 272 Xian(indica)*** panel showed substantial variation for all traits in the two environments and revealed complex phenotypic correlations.A total of 70 QTL influencing the 11 traits were identified using 469,377 high-quality SNP *** QTL were detected consistently in four chromosomal regions in both *** QTL clusters simultaneously affected source,sink,source–sink relationship,and grain yield traits,probably explaining the genetic basis of significant correlations of grain yield with source and sink *** selected 24 candidate genes in the four consistent QTL regions by identifying linkage disequilibrium(LD)blocks associated with significant SNPs and performing haplotype *** genes included one cloned gene(NOG1)and three newly identified QTL(qHI6,qTGW7,and qFLA8).These results provide a theoretical basis for high-yield rice breeding by increasing and balancing source–sink relationships using marker-assisted selection.

关键词： Rice GWAS Source–sink relationship Quantitative trait loci/locus(QTL) Candidate gene

Developing green super rice varieties with high nutrient use efficiency by phenotypic selection under varied nutrient conditions

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The Crop Journal 2019年第3期7卷 368-377页

作者： Zilhas Ahmed Jewel Jauhar Ali yunlong pang Anumalla Mahender Bart Acero Jose Hernandez Jianlong Xu Zhikang Li Rice Breeding Platform International Rice Research Institute (IRRI)Los BanosLaguna 4031Philippines Institute of Crop Sciences Chinese Academy of Agricultural ScienceBeijing 100081China Institute of Crop Science College of Agriculture and Food ScienceUniversity of the PhilippinesLos BanosLaguna 4031Philippines

The development of green super rice varieties with improved nutrient use efficiency(NuUE)is a vital target area to increase yield and make it more stable under rainfed *** the present study, we followed an early backcross(BC) breeding approach by using a highyielding and widely adapted Xian variety, Weed Tolerant Rice 1(WTR-1), as a recipient and a Geng variety, Hao-An-Nong(HAN), as a *** from the BC1F2 generation, the BC population went through one generation of selection under irrigated, low-input, and rainfed conditions, followed by four consecutive generations of screening and selection for high grain yield(GY) under six different nutrient conditions(NPK, 75 N,-N,-P,-NP, and-NPK), leading to the development of 230 BC1F6 introgression lines(ILs).These 230 ILs were evaluated under the same six nutrient conditions for 13 agro-morphological and grain yield component traits in comparison to four checks and *** trait variations were observed between the treatments and *** correlations were identified for GY with biomass, panicle length, flag-leaf area, flag-leaf width, filled grain number per panicle,1000-grain weight, and tiller number under-N,-P,-NP, and-NPK *** of 230 ILs,12 were identified as promising under two or more nutrient deficiency *** results demonstrated an efficient inter-subspecific BC breeding procedure with a first round of selection under rainfed-drought conditions, followed by four generations of progeny testing for yield performance under six nutrient *** promising ILs can be useful resources for molecular genetic dissection and understanding the physiological mechanisms of NuUE.

关键词： Nutrient use efficiency Grain yield Nitrogen Phosphorus Green super rice

Effects of repeated applications of fungicide carbendazim on its persistence and microbial community in soil

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Journal of Environmental Sciences 2009年第2期21卷 179-185页

作者： YU yunlong,CHU Xiaoqiang,pang Guohui,XIANG Yueqin,FANG Hua Department of Plant Protection,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310029,China. Department of Plant Protection College of Agriculture and Biotechnology Zhejiang University Hangzhou 310029 China

Carbendazim, a systemic benzimidazole fungicide, is applied repeatedly to control plant diseases including soilborne diseases, over a growing season. Studies were carried out under laboratory conditions to assess the effects of repeated carbendazim applications on its persistence and microbial community in soil. The results indicate that dissipation of carbendazim in soil was accelerated with its application frequency. The degradation rate constant of carbendazim was increased significantly from 0.074 d-1 to 0.79 d-1. The corresponding half-life was shorten markedly from 9.3 d to 0.9 d after four repeated applications. No significant inhibitory effect of carbendazim on soil microbial utilization of the carbon sources was observed after first treatment, but a slight increase in average well color development （AWCD） was shown after second, third, and fourth applications. It suggested that soil microorganisms become adapted to carbendazim after repeated application. Simpson and Shannon indexes of soil microbial community from carbendazim treated soil were also similar to those from the control soil, indicating that the richness and dominant character of soil microorganisms remain unchangeable after repeated application. However, after first, second, and third addition of carbendazim, McIntosh indexes on day 21 were significantly higher compared with the control, suggesting that balance of soil microorganisms was altered due to the enrichment of the specific carbendazim-adapting strains in soil

关键词： carbendazim degradation soil microorganism repeated application

Genetic background effects on QTL and QTL × environment interaction for yield and its component traits as revealed by reciprocal introgression lines in rice

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The Crop Journal 2014年第6期2卷 345-357页

作者： Xiaoqian Wang yunlong pang Jian Zhang Qiang Zhang Yonghong Tao Bo Feng Tianqing Zheng Jianlong Xu Zhikang Li Institute of Crop Science/National Key Facility for Crop Gene Resources and Genetic Improvement Chinese Academy of Agricultural Sciences

QTLs for quantitative traits are influenced by genetic background(GB) and *** of QTL with GB independency and environmental stability is prerequisite for effective marker-assisted selection(MAS). In this study, QTLs and QTL × environment interactions affecting grain yield per plant(GY) and its component traits, filled grain number per panicle(FGN), panicle number per plant(PN) and 1000-grain weight(TGW) across six environments were dissected using two sets of reciprocal introgression lines(ILs) derived from the cross Lemont × Teqing and SNP genotypic data. ANOVA indicated that the differences among genotypes and environments within each set of ILs were highly significant for all traits. A total of 72 distinct QTLs for GY and its component traits including 15 for GY, 25 for FGN, 18 for PN, and 29 for TGW were detected over the six environments. Most QTLs(87.4%) showed significant QTL × environment interactions(QEIs) and appeared to be more or less environment-specific. Among 72 QTLs, 15(20.8%) QTLs and 12(16.7%) QEIs were commonly identified in both backgrounds, indicating QTL especially QEI for yield and its component traits had strong GB effects. Four QTL regions affecting GY and its component traits, including S1269707–S4288071, S16661497–S17511092, and S35861863–S36341768 on chromosome 3, and S4134205–S7643153 on chromosome 5, were detected in both backgrounds and coincided with cloned genes for yield-related traits. These regions can be the targeted in rice breeding for high yield potential through MAS. Application of QTL main effects and their environmental interaction effects in MAS was discussed in detail.

关键词： Quantitative trait locus Yield potential Marker-assisted selection Genetic background QTL × environment interaction

Construction of dominant nuclear male sterility recurrent selection population in rice and its screening of salt tolerance plants

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Construction of dominant nuclear male sterility recurrent se...

High-Resolution Genome-wide Association Study Identifies Genomic Regions and Candidate Genes for Important Agronomic Traits in Wheat

第十四届全国植物基因组学大会

作者： yunlong pang Jianlong Xu Zhikang Li Institute of Crop Science Chinese Academy of Agricultural Science

Recurrent selection is based on a population which has very abundant genetic basis, through several cycle of "selection—crossing-selection",breaking the genetic linkage between favorable and unfavorable genes,pyramid... 详细信息

关键词： male sterility recurrent selection introgression Lines salt tolerance

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Molecular Plant 2020年第9期13卷 1311-1327页

作者： yunlong pang Chunxia Liu Danfeng Wang Paul St.Amand Amy Bernardo Wenhui Li Fang He Linzhi Li Liming Wang Xiufang Yuan Lei Dong Yu Su Huirui Zhang Meng Zhao yunlong Liangi Hongze Jia Xitong Shen Yue Lu Hongming Jiang Yuye Wu Anfei Li Honggang Wang Lingrang Kong Guihua Bai Shubing Liu State Key Laboratory of Crop Biology College of AgronomyShandong Agricultural UniversityTai'an 271018China USDA-ARS Hard Winter Wheat Genetics Research UnitManhattanKS 66506USA Department of Plant Pathology Kansas State UniversityManhattanKS 66506USA Yantai Academy of Agricultural Sciences Yantai 265500China College of Agriculture Henan University of Science and TechnologyLuoyang 471000China College of Agriculture Guizhou UniversityGuiyang 550025China

Wheat(Triticum aestivum)is a major staple food crop *** dissection of important agronomic traits is essential for continuous improvement of wheat yield to meet the demand of the world's growing *** conducted a large-scale genome-wide association study(GWAS)using a panel of 768 wheat cultivars that were genotyped with 327609 single-nucleotide polymorphisms generated by genotyping-by-sequencing and detected 395 quantitative trait loci(QTLs)for 12 traits under 7 *** them,273 QTLs were delimited to≤1.0-Mb intervals and 7 of them are either known genes(Rht-D,Vrn-B1,and Vrn-D1)that have been cloned or known QTLs(TaGA2ox8,APO1,TaSus1-7B,and Rht12)that were previously *** putative candidate genes were identified for three QTLs that enhance spike seed setting and grain size using gene expression data and were validated in three bi-parental *** sequence analysis identified 33 putative wheat orthologs that have high identity with rice genes in QTLs affecting similar *** r^2 values for additive effects observed among the QTLs for most traits indicated that the phenotypes of these identified QTLs were highly *** from this study demonstrated that significantly increasing GWAS population size and marker density greatly improves detection and identification of candidate genes underlying a QTL,solidifying the foundation for large-scale QTL fine mapping,candidate gene validation,and developing functional markers for genomics-based breeding in wheat.

关键词： aestivum) Wheat breeding

Multifunction-oriented high-mobility polymer semiconductors

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National Science Review 2024年第3期11卷 16-19页

作者： Mingliang Zhu yunlong Guo Yunqi Liu Beijing National Laboratory for Molecular Sciences Organic Solids Laboratory Institute of Chemistry Chinese Academy of Sciences School of Chemical Sciences University of Chinese Academy of Sciences

The discovery of conductive polyacetylene has initiated interdisciplinary research into condensed matter physics and synthetic chemistry in organic electronics. Unlike their inorganic and small-molecule counterparts, polymer semiconductors possess the inherent advantages of facile solution processing,large-area manufacturing, excellent mechanical strength and intrinsic flexibility.

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