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Journal of Integrative Plant Biology 2013年第11期55卷 1038-1053页

作者： Xiaohua Sun Andan Zhu Shuzhen Liu Ling Sheng Qiaoli Ma Li Zhang Elsayed Mohamed Elsayed Nishawy yunliu zeng Juan Xu Zhaocheng Ma Yunjiang Cheng Xiuxin Deng Key Laboratory of Horticultural Plant Biology Ministry of Education Huazhong Agricultural University

Citric acid plays an important role in fresh fruit flavor and its adaptability to post-harvest storage conditions. In order to explore organic acid regulatory mechanisms in post-harvest citrus fruit, systematic biological analyses were conducted on stored Hirado Buntan Pummelo （HBP; Citrus grandis） fruits. High- performance capillary electrophoresis, subcellular organelle expression microarray, real-time quantitative reverse transcription polymerase chain reaction, gas chromatography mass spectrometry （GC-MS）, and conventional physiological and biochemical analyses were undertaken. The results showed that the concentration of organic acids in HBP underwent a regular fluctuation. GC-MS-based metabolic profiling indicated that succinic acid, ~,-aminobutyric acid （GABA）, and glutamine contents increased, but 2- oxoglutaric acid content declined, which further confirmed that the GABA shunt may have some regulatory roles in organic acid catabolism processes. In addition, the concentration of organic acids was significantly correlated with senescence-related physiological processes, such as hydrogen peroxide content as well as superoxide dismutase and peroxidase activities, which showed that organic acids could be regarded as important parameters for measuring citrus fruit post-harvest senescence processes.

关键词： Citrus gene expression metabolite organic acids post-harvest storage.

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猕猴桃大帐气调气流组织的数值模拟及优化

农业工程学报 2024年第5期40卷 307-316页

作者：徐勤超单程陈昊义朱家庭曾云流李善军潘海兵张文东华中农业大学工学院农业农村部长江中下游农业装备重点实验室武汉430070 华中农业大学园艺林学学院武汉430070 微呼吸(武汉)农业科技有限公司武汉430070

为确定合理的猕猴桃大帐气调参数,提高大帐气调贮藏性能,该研究通过模拟猕猴桃在果筐内的自然堆叠状态,建立了单个猕猴桃尺度的大帐气调微环境模型,采用数值模拟方法,分析猕猴桃大帐气调过程中N_(2)纯度、流量、管径、出口高度4个关键... 详细信息

为确定合理的猕猴桃大帐气调参数,提高大帐气调贮藏性能,该研究通过模拟猕猴桃在果筐内的自然堆叠状态,建立了单个猕猴桃尺度的大帐气调微环境模型,采用数值模拟方法,分析猕猴桃大帐气调过程中N_(2)纯度、流量、管径、出口高度4个关键参数对降氧时间及气体分布均匀性的影响。基于Box-Behnken原理设计四因素三水平正交仿真试验,利用Design-Expert软件建立响应指标回归模型,对所选取的4个因素进行响应面分析和参数优化。对优化参数组合进行仿真试验,对比优化参数组合条件下响应面回归模型预测值与仿真试验数值模拟值。结果表明:各因素对降氧时间影响由大到小顺序为出气口高度、流量、N_(2)纯度、管径;对不均匀系数影响由大到小顺序为N_(2)纯度、出气口高度、管径、流量。猕猴桃大帐气调的最优气调参数组合为N_(2)纯度96.0%、流量23.0 m^(3)/h、管径50 mm、出气口高度3 126 mm,对应的降氧时间、不均匀系数分别为989 s、6.1%。降氧时间响应面回归模型预测值与仿真值之间的相对误差为3.1%,不均匀系数响应面回归模型预测值与仿真值之间的相对误差为8.4%。研究对于大帐气调装备的研发及性能提升具有重要的理论和实际意义。

关键词：数值模拟响应面法贮藏气调性能大帐气调

Natural Variation in CCD4 Promoter Underpins Species-Specific Evolution of Red Coloration in Citrus Peel

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Molecular Plant 2019年第9期12卷 1294-1307页

作者： Xiongjie Zheng Kaijie Zhu Quan Sun Weiyi Zhang Xia Wang Hongbo Cao Meilian Tan Zongzhou Xie yunliu zeng Junli Ye Lijun Chai Qiang Xu Zhiyong Pan Shunyuan Xiao Paul D.Fraser Xiuxin Deng Key Laboratory of Horticultural Plant Biology(Ministry of Education) Huazhong Agricultural University.WuhanChina School of Biological Sciences.Royal Holloway University of LondonEghamSurrey TW20 OEXUK Department of Plant Science and Landscape Architecture Institute for Bioscience and Biotechnology ResearchUniversity of MarylandRockvilleMDUSA Present address:College of Horticulture Agricultural University of Hebei.Baoding Hebei 071000China Present address:Key Laboratory of Biology and Genetic Improvement of Oil Crops Ministry of Agriculture.Oil Crops Research Institute of the Chinese Academy of Agricultural SciencesWuhan 430070China

Carotenoids and apocarotenoids act as phytohormones and volatile precursors that influence plant development and confer aesthetic and nutritional value critical to consumer preference.Citrus fruits display considerable natural variation in carotenoid and apocarotenoid pigments.In this study,using an integrated genetic approach we revealed that a 5;c/s-regulatory change at CCD4b encoding CAROTENOID CLEAVAGE DIOXYGENASE 4b is a major genetic determinant of natural variation in C3 0 apocarotenoids responsible for red coloration of citrus peel.Functional analyses demonstrated that in addition the known role in synthesizing 3-citraurin,CCD4b is also responsible for the production of another important C3 0 apocarotenoid pigment,p-citraurinene.Furthermore,analyses of the CCD4b promoter and transcripts from various citrus germplasm accessions established a tight correlation between the presence of a putative 5'c/s-regulatory enhancer within an MITE transposon and the enhanced allelic expression of CCD4b in C3 0 apocarotenoid-rich red-peeled accessions.Phylogenetic analysis provided further evidence that functional diversification of CCD4b and naturally occurring variation of the CCD4b promoter resulted in the stepwise evolution of red peels in mandarins and their hybrids.Taken together,our findings provide new insights into the genetic and evolutionary basis of apocarotenoid diversity in plants,and would facilitate breeding efforts that aim to improve the nutritional and aesthetic value of citrus and perhaps other fruit crops.

关键词： citrus apocarotenoid natural variation carotenoid cleavage dioxygenase promoter transposon

NEW INSIGHTS INTO THE PHYLOGENY AND SPECIATION OF KUMQUAT (FORTUNELLA SPP.) BASED ON CHLOROPLAST SNP, NUCLEAR SSR AND WHOLE-GENOME SEQUENCING

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Frontiers of Agricultural Science and Engineering 2022年第4期9卷 627-641页

作者： Chenqiao ZHU Peng CHEN Junli YE Hang LI Yue HUANG Xiaoming YANG Chuanwu CHEN Chenglei ZHANG Yuantao XU Xiaoli WANG Xiang YAN Guangzhou DENG Xiaolin JIANG Nan WANG Hongxing WANG Quan SUN Yun LIU Di FENG Min YU Xietian SONG Zongzhou XIE yunliu zeng Lijun CHAI Qiang XU Chongling DENG Yunjiang CHENG Xiuxin DENG Key Laboratory of Horticultural Plant Biology(Ministry of Education) Huazhong Agricultural UniversityWuhan 430070China Fruit Industry Technology Guidance Stand of Ganzhou Ganzhou 341000China Guangxi Academy of Specialty Crops/Guangxi Citrus Breeding and Cultivation Research Center of Engineering Technology Guilin 541004China Ganzhou Citrus Research Institute Ganzhou 341000China

Kumquat(Fortunella spp.)is a fruit and ornamental crop worldwide due to the palatable taste and high ornamental value of its fruit.Although Fortunella is classified into the economically important true citrus fruit tree group together with Citrus and Poncirus,few studies have been focused on its evolutionary scenario.In this study,analysis of five chloroplast loci and 47 nuclear microsatellites(nSSR)loci from 38 kumquat and 10 citrus accessions revealed the independent phylogeny of Fortunella among citrus taxa,and that Fortunella mainly comprises two populations:CUL,cultivated Fortunella spp.(F.margarita,F.crassifolia and F.japonica);and HK,wild Hong Kong kumquat(Fortunella hindsii).Genomic analysis based on whole-genome SNPs indicated that the allele frequency of both pupations deviated from the neutral selection model,suggesting directional selection was a force driving their evolutions.CUL exhibited lower genomic diversity and higher linkage strength than HK,suggesting artificial selection involved in its origin.A high level of genetic differentiation(Fst=0.364)was detected and obviously asynchronous demographic changes were observed between CUL and HK.Based on these results,a new hypothesis for the speciation of Fortunella is proposed.

关键词： Citrus Fortunella kumquat phylogenetics

Isolation and comparative proteomic analysis of mitochondria from the pulp of ripening citrus fruit

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Horticulture Research 2021年第1期8卷 403-418页

作者： Xin Li Yingfang Chai Hongbin Yang Zhen Tian Chengyang Li Rangwei Xu Chunmei Shi Feng Zhu yunliu zeng Xiuxin Deng Pengwei Wang Yunjiang Cheng National R&D Centre for Citrus Preservation Key Laboratory of Horticultural Plant Biology(Ministry of Education)College of Horticulture and Forestry ScienceHuazhong Agricultural UniversityWuhan 430070People’s Republic of China

Mitochondria are crucial for the production of primary and secondary metabolites,which largely determine the quality of fruit.However,a method for isolating high-quality mitochondria is currently not available in citrus fruit,preventing high-throughput characterization of mitochondrial functions.Here,based on differential and discontinuous Percoll density gradient centrifugation,we devised a universal protocol for isolating mitochondria from the pulp of four major citrus species,including satsuma mandarin,ponkan mandarin,sweet orange,and pummelo.Western blot analysis and microscopy confirmed the high purity and intactness of the isolated mitochondria.By using this protocol coupled with a label-free proteomic approach,a total of 3353 nonredundant proteins were identified.Comparison of the four mitochondrial proteomes revealed that the proteins commonly detected in all proteomes participate in several typical metabolic pathways(such as tricarboxylic acid cycle,pyruvate metabolism,and oxidative phosphorylation)and pathways closely related to fruit quality(such asγ-aminobutyric acid(GABA)shunt,ascorbate metabolism,and biosynthesis of secondary metabolites).In addition,differentially abundant proteins(DAPs)between different types of species were also identified;these were found to be mainly involved in fatty acid and amino acid metabolism and were further confirmed to be localized to the mitochondria by subcellular localization analysis.In summary,the proposed protocol for the isolation of highly pure mitochondria from different citrus fruits may be used to obtain high-coverage mitochondrial proteomes,which can help to establish the association between mitochondrial metabolism and fruit storability or quality characteristics of different species and lay the foundation for discovering novel functions of mitochondria in plants.

关键词： orange purity oxidative

A comprehensive proteomic analysis of elaioplasts from citrus fruits reveals insights into elaioplast biogenesis and function

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Horticulture Research 2018年第1期5卷 879-889页

作者： Man Zhu Jiajia Lin Junli Ye Rui Wang Chao Yang Jinli Gong Yun Liu Chongling Deng Ping Liu Chuanwu Chen Yunjiang Cheng Xiuxin Deng yunliu zeng Key Laboratory of Horticultural Plant Biology(Ministry of Education) College of Horticulture and Forestry ScienceHuazhong Agricultural UniversityWuhan430070China Institute of Citrus Science Huazhong Agricultural UniversityWuhan430070China Shanghai Applied Protein Technology Co.Ltd Shanghai200233China Guangxi Citrus Research Institute Guangxi541004China

Elaioplasts of citrus peel are colorless plastids which accumulate significant amounts of terpenes.However,other functions of elaioplasts have not been fully characterized to date.Here,a LC–MS/MS shotgun technology was applied to identify the proteins from elaioplasts that were highly purified from young fruit peel of kumquat.A total of 655 putative plastid proteins were identified from elaioplasts according to sequence homology in silico and manual curation.Based on functional classification via Mapman,~50%of the identified proteins fall into six categories,including protein metabolism,transport,and lipid metabolism.Of note,elaioplasts contained ATP synthase and ADP,ATP carrier proteins at high abundance,indicating important roles for ATP generation and transport in elaioplast biogenesis.Additionally,a comparison of proteins between citrus chromoplast and elaioplast proteomes suggest a high level of functional conservation.However,some distinctive protein profiles were also observed in both types of plastids notably for isoprene biosynthesis in elaioplasts,and carotenoid metabolism in chromoplasts.In conclusion,this comprehensive proteomic study provides new insights into the major metabolic pathways and unique characteristics of elaioplasts and chromoplasts in citrus fruit.

关键词： citrus comprehensive metabolism

Integrated transcriptomic and metabolomic analyses of a wax deficient citrus mutant exhibiting jasmonic acid-mediated defense against fungal pathogens

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Horticulture Research 2018年第1期5卷 404-417页

作者： Yizhong He Jingwen Han Runsheng Liu Yuduan Ding Jinqiu Wang Li Sun Xiaoming Yang yunliu zeng Weiwei Wen Juan Xu Hongming Zhang Xiang Yan Zhaoxing Chen Zuliang Gu Hong Chen Huanqing Tang Xiuxin Deng Yunjiang Cheng Key Laboratory of Horticultural Plant Biology Ministry of EducationHuazhong Agricultural UniversityWuhan430070China Institute of Citrus Science Research of Ganzhou Ganzhou341000Jiangxi ProvinceChina Research Center of Navel Orange Planting Technology of Anyuan County Ganzhou341000Jiangxi ProvinceChina

Naturally,resistant crop germplasms are important resources for managing the issues of agricultural product safety and environment deterioration.We found a spontaneous mutant of‘Newhall’navel orange(Citrus sinensis Osbeck)(MT)with broad-spectrum protections against fungal pathogens in the orchard,postharvest-storage,and artificial inoculation conditions.To understand the defense mechanism of MT fruit,we constructed a genome-scale metabolic network that integrated metabolome and transcriptome datasets.The coordinated transcriptomic and metabolic data were enriched in two sub-networks,showing the decrease in very long chain fatty acid(by 41.53%)and cuticular wax synthesis(by 81.34%),and increase in the synthesis of jasmonic acid(JA)(by 95.23%)and JA-induced metabolites such as 5-dimethylnobietin(by 28.37%)in MT.Furthermore,cytological and biochemical analyses confirmed that the response to fungal infection in MT was independent of wax deficiency and was correlated with the levels of jasmonates,and the expression of plant defensin gene PDF1.2.Results of exogenous application of MeJA and JA inhibitors such as propyl gallate proved that JA-mediated defense contributes to the strong tolerance against pathogens in MT.Our results indicated that jasmonate biosynthesis and signaling are stimulated by the fatty acid redirection of MT,and participate in the tolerance of pathogenic fungi.

关键词： synthesis orange deficient

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气调保鲜技术在猕猴桃贮藏保鲜中的应用进展

园艺学报 2023年第9期50卷 1916-1928页

作者：何斌徐勤超戢小梅王小玲张文东程运江曾云流华中农业大学园艺林学学院果蔬园艺作物种质创新与利用全国重点实验室国家柑橘保鲜技术研发专业中心武汉430070 华中农业大学工学院武汉430070 武汉市农业科学院武汉430075 江西省科学院生物资源研究所南昌330096 微呼吸(武汉)农业科技有限公司武汉430070

气调贮藏是一种通过改变贮藏气体环境,有效延长猕猴桃等果蔬贮藏寿命与货架期的高效保鲜技术,但是由于气调设备昂贵、管理技术复杂、设备与参数研究滞后、市场过度竞争等问题,气调贮藏在中国果蔬保鲜上始终未能得到广泛有效地应用。因此... 详细信息

气调贮藏是一种通过改变贮藏气体环境,有效延长猕猴桃等果蔬贮藏寿命与货架期的高效保鲜技术,但是由于气调设备昂贵、管理技术复杂、设备与参数研究滞后、市场过度竞争等问题,气调贮藏在中国果蔬保鲜上始终未能得到广泛有效地应用。因此,本文中以猕猴桃果实为例,综述了气调贮藏对果实品质的影响,详述了使用气调贮藏时需控制的参数,梳理了气调保鲜技术在猕猴桃贮藏保鲜中的应用情况,最后针对在实际应用中存在的问题做出展望,以期为猕猴桃等园艺产品气调保鲜技术的发展与应用提供指导。

关键词：猕猴桃采后气调保鲜

香石竹DcERF-1转录因子对切花衰老的负调控作用

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园艺学报 2022年第6期49卷 1313-1326页

作者：王妍孙政冯珊袁心怡仲林林曾云流傅小鹏程运江包满珠张帆华中农业大学园艺林学学院园艺植物生物学教育部重点实验室武汉430070 国家柑橘保鲜技术研发专业中心武汉430070 湖北洪山实验室武汉430070 华中农业大学园艺林学学院农业农村部华中都市农业重点实验室武汉430070

以香石竹(Dianthus caryophyllus L.)为材料,克隆得到1个ERF转录因子基因,命名为DcERF-1。氨基酸序列比对及系统进化树分析发现,DcERF-1与拟南芥AtERF-1的同源性最高,属于Ⅸ(B3)亚组。实时荧光定量PCR显示,DcERF-1表达量在花中最高,且... 详细信息

以香石竹(Dianthus caryophyllus L.)为材料,克隆得到1个ERF转录因子基因,命名为DcERF-1。氨基酸序列比对及系统进化树分析发现,DcERF-1与拟南芥AtERF-1的同源性最高,属于Ⅸ(B3)亚组。实时荧光定量PCR显示,DcERF-1表达量在花中最高,且在花瓣自然衰老和乙烯诱导的衰老过程中都呈现先上升后下降的趋势。DcERF-1定位于细胞核中。DcERF-1在香石竹花瓣中瞬时过量表达后,花瓣褪色速度明显延缓,离子渗透率明显降低;DcERF-1被瞬时沉默后,花瓣褪色速度显著加快,离子渗透率显著升高,衰老标志基因DcSAG12表达量显著上调。酵母单杂交试验证明乙烯信号转导途径核心转录因子DcEIN3能直接结合DcERF-1的启动子。双荧光素酶瞬时表达试验证明DcERF-1能抑制乙烯生物合成途径中ACC氧化酶基因DcACO4的表达活性。综合结果表明DcERF-1负调控香石竹切花衰老。

关键词：香石竹切花衰老乙烯采后 ERF转录因子转录调控

结合三维卷积神经网络和Haar小波滤波器的高光谱猕猴桃品质分类

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激光与光电子学进展 2023年第20期60卷 41-50页

作者：金科郭志强曾云流丁港武汉理工大学信息工程学院湖北武汉430070 华中农业大学园艺林学学院园艺植物生物学教育部重点实验室国家柑橘保鲜技术研发专业中心湖北武汉430070

针对猕猴桃硬度品质无损检测分类困难的问题,提出了结合高光谱成像技术和卷积神经网络的分类模型。该模型融合Haar小波核提取的空间特征信息和三维卷积核提取的空谱联合信息,采用分解数据通道连接的方式确保所有特征能够流到模型末尾,... 详细信息

针对猕猴桃硬度品质无损检测分类困难的问题,提出了结合高光谱成像技术和卷积神经网络的分类模型。该模型融合Haar小波核提取的空间特征信息和三维卷积核提取的空谱联合信息,采用分解数据通道连接的方式确保所有特征能够流到模型末尾,提升了网络特征提取的能力。通过自制的猕猴桃硬度品质Kiwi_seed数据集上的实验表明,Haar小波变换模块可以显著提升网络的特征提取能力;通过消融实验表明,在增加Haar小波变换模块后模型的分类准确率提升了7.4%,最优可达97.3%,优于经典的图像分类网络,可以很好地解决猕猴桃硬度品质的无损检测分类问题。

关键词：高光谱图像猕猴桃图像分类卷积神经网络 Haar小波变换