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Insight into the characteristics of an important evolutionary model bird(Geospiza magnirostris)mitochondrial genome through comparison

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BIOCELL 2022年第7期46卷 1733-1746页

作者： ZHENGGANG XU LIANG WU JIAHAO CHEN yunlin ZHAO CHONGXUAN han TIAN HUANG GUIYAN YANG Key Laboratory of National Forestry and Grassland Administration on Management of Western Forest Bio-Disaster College of ForestryNorthwest A&F UniversityYangling712100China Engineering Research Center for Internet of Animals Changsha410000China Key Laboratory of Forestry Remote Sensing Based Big Data&Ecological Security for Hunan Province Central South University of Forestry and TechnologyChangsha410004China

Darwin’s finches are the most classic case of evolution.Early studies on the evolution of this species were mainly based on morphology.Until now,the mitochondrial genome of Geospiza magnirostris has been sequenced and the study explored the characteristics of the complete genome of G.magnirostris and verified the evolutionary position of it.The 13 PCGs initiated by ATN codons.The stop codons of three PCGs(ND2,COX3 and ND4)were incomplete,with only T-or TA-replacing complete form TAA or TAG.All the tRNA genes expressed a typical cloverleaf secondary structure,except for tRNA^(Ser1)(AGY),whose dihydrouridine(DHU)arm was lack and instead with a simple loop.In the sequence of the control region of G.magnirostris,we found six simple repeat tandem sequences with a total length of 42 bp.Two characteristic conserved overlapping junction(ATGCTAA)and(CAAGAAAG)were observed as reported for eight selected Passeriformes birds.A special conserved overlapping junction(ATCTTACC)involved in mitochondrial transcription termination was found between tRNA^(Tyr) and COX1 in G.magnirostris’s control region.Four most frequently used amino acids in G.magnirostris’s PCGs were Leu1(CUN),Ile,Thr,Ala.The codon usage of G.magnirostris was relatively average,and there was no particular bias.The ratio Ka/Ks results showed that G.magnirostris receives less natural selection pressure.The phylogenetic relationships and cluster analysis of relative codon usage showed that G.magnirostris and Thraupis episcopus clustered in one branch.The phylogenetic position of G.magnirostris was consistent with the traditional taxonomic of Thraupis.The results supported the conclusion that G.magnirostris belongs to the morphological classification of the family Thraupidae.

关键词： Geospiza magnirostris Mitochondrial genome Comparative analysis Evolution analysis Phylogenetic relationships

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Microarray microRNA profiling of urinary exosomes in a 5XFAD mouse model of Alzheimer’s disease

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Animal Models and Experimental Medicine 2021年第3期4卷 233-242页

作者： Zhiqi Song Yajin Qu Yanfeng Xu Ling Zhang Li Zhou Yunlin Han Wenjie Zhao Pin Yu Yu Zhang Xianglei Li Chuan Qin Key Laboratory of Human Disease Comparative Medicine Chinese Ministry of HealthBeijing Key Laboratory for Animal Models of Emerging and Remerging Infectious DiseasesInstitute of Laboratory Animal ScienceChinese Academy of Medical Sciences and Comparative Medicine CenterPeking Union Medical CollegeBeijingChina

Background:Alzheimer's disease(AD)is an incurable and irreversible neurodegen-erative disease,without a clear pathogenesis.Therefore,identification of candidates before amyloid-βplaque(Aβ)deposition proceeds is of major significance for earlier intervention in AD.Methods:To explore the potential noninvasive earlier biomarkers of AD in a 5XFAD mouse model,microRNAs(miRNAs)from urinary exosomes in 1-month-old pre-Aβaccumulation 5XFAD mice models and their littermate controls were profiled by mi-croarray analysis.The differentially expressed miRNAs were further analyzed via droplet digital PCR(ddPCR).Results:Microarray analysis demonstrated that 48 differentially expressed miRNAs(18 upregulated and 30 downregulated),of which six miRNAs-miR-196b-5p,miR-339-3p,miR-34a-5p,miR-376b-3p,miR-677-5p,and miR-721-were predicted to display gene targets and important signaling pathways closely associated with AD pathogenesis and verified by ddPCR.Conclusions:Urinary exosomal miRNAs showing differences in expression prior to Aβ-plaque deposition were identified.These exosomal miRNAs represent potential noninvasive biomarkers that may be used to prevent AD in clinical applications.

关键词： 5XFAD mouse model Alzheimer's disease biomarkers microarray miRNA urinary exosome

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一种用于治疗膀胱癌且并发症较少的改良回肠导管术

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癌症 2019年第10期38卷 463-472页

作者： Zhiyong Li Zhuowei Liu Kai Yao Zike Qin Hui han Yonghong Li Pei Dong yunlin Ye Yanjun Wang Zhiming Wu Zhiling Zhang Fangjian Zhou 泌尿外科华南肿瘤学国家重点实验室肿瘤医学协同创新中心中山大学肿瘤防治中心广东广州510060

背景与目的根治性膀胱切除术和尿流改道术仍然是肌层浸润性或高危或复发非肌层浸润性膀胱癌患者的标准外科治疗方法。尽管这种方法显著延长了患者的生存期,但仍存在与尿流改道相关的术后并发症。本研究旨在通过与传统回肠导管尿流改道... 详细信息

背景与目的根治性膀胱切除术和尿流改道术仍然是肌层浸润性或高危或复发非肌层浸润性膀胱癌患者的标准外科治疗方法。尽管这种方法显著延长了患者的生存期,但仍存在与尿流改道相关的术后并发症。本研究旨在通过与传统回肠导管尿流改道术相比较,评价改良回肠导管术减少早期及晚期造口及输尿管回肠吻合术相关并发症的疗效。方法回顾分析2000年1月1日至2016年6月30日在中山大学肿瘤防治中心接受膀胱癌根治术及回肠导管尿流改道术的膀胱癌患者的临床资料,患者分别采用了传统或改良的回肠导管术。采用t检验和卡方检验,比较传统与改良回肠导管术组患者的临床病理学特征。采用多变量logistic回归分析和多变量Cox回归分析,确定两组发生造口和输尿管回肠吻合相关并发症的概率。结果接受改良与传统回肠导管术的患者分别为145例与100例。两组的临床病理学特征具有可比性。改良回肠导管术组与传统回肠导管术组相比,造口相关并发症发生率显著降低(0.7%vs.17.0%,P<0.001)。改良回肠导管术组未发现晚期造口相关并发症,但传统回肠导管术组有13(13.0%)例患者出现并发症。改良回肠导管术组与传统回肠导管术组相比,输尿管回肠吻合相关并发症发生率显著降低(4.8%vs.15.0%,P=0.001)。在多变量分析中,与传统回肠导管术组相比,改良回肠导管术组更不容易出现造口[比值比(odds ratio,OR)=0.024,95%置信区间(confidence interval,CI):0.003–0.235;P=0.001]或输尿管回肠吻合术相关并发症(OR=0.141,95%CI:0.042–0.476;P=0.002)。结论改良的回肠导管尿流改道可有效减少与造口及输尿管回肠吻合术相关的早期及晚期并发症。需要前瞻性随机临床试验来证实我们的结果。

关键词：膀胱癌膀胱切除术尿流改道回肠导管术并发症手术方法

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Integrated histopathological,lipidomic,and metabolomic profiles reveal mink is a useful animal model to mimic the pathogenicity of severe COVID-19 patients

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Signal Transduction and Targeted Therapy 2022年第2期7卷 560-572页

作者： Zhiqi Song Linlin Bao Wei Deng Jiangning Liu Erjun Ren Qi Lv Mingya Liu Feifei Qi Ting Chen Ran Deng Fengdi Li Yunpeng Liu Qiang Wei Hong Gao Pin Yu Yunlin Han Wenjie Zhao Junjun Zheng Xujian Liang Fuhe Yang Chuan Qin NHC Key Laboratory of Human Disease Comparative Medicine Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious DiseasesInstitute of Laboratory Animal ScienceChinese Academy of Medical Sciences and Comparative Medicine CenterPeking Union Medical CollegeBeijingChina Shi Jia Zhuang Academy Of Agricultural And Forestry Sciences ShijiazhuangChina Institute of Special Animal and Plant Sciences of CAAS ChangchunChina Chinese Academy of Agricultural Sciences(CAAS) BeijingChina

Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is transmitted on mink farms between minks and humans in many countries.However,the systemic pathological features of SARS-CoV-2-infected minks are mostly unknown.Here,we demonstrated that minks were largely permissive to SARS-CoV-2,characterized by severe and diffuse alveolar damage,and lasted at least 14 days post inoculation(dpi).We first reported that infected minks displayed multiple organ-system lesions accompanied by an increased inflammatory response and widespread viral distribution in the cardiovascular,hepatobiliary,urinary,endocrine,digestive,and immune systems.The viral protein partially co-localized with activated Mac-2+macrophages throughout the body.Moreover,we first found that the alterations in lipids and metabolites were correlated with the histological lesions in infected minks,especially at 6 dpi,and were similar to that of patients with severe and fatal COVID-19.Particularly,altered metabolic pathways,abnormal digestion,and absorption of vitamins,lipids,cholesterol,steroids,amino acids,and proteins,consistent with hepatic dysfunction,highlight metabolic and immune dysregulation.Enriched kynurenine in infected minks contributed to significant activation of the kynurenine pathway and was related to macrophage activation.Melatonin,which has significant anti-inflammatory and immunomodulating effects,was significantly downregulated at 6 dpi and displayed potential as a targeted medicine.Our data first illustrate systematic analyses of infected minks to recapitulate those observations in severe and fetal COVID-19 patients,delineating a useful animal model to mimic SARS-CoV-2-induced systematic and severe pathophysiological features and provide a reliable tool for the development of effective and targeted treatment strategies,vaccine research,and potential biomarkers.

关键词： patients alterations vitamin

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微波法快速可控合成红光发射的NaYF_(4):Yb^(3+)/Er^(3+)上转换纳米材料

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功能材料 2022年第9期53卷 9091-9097页

作者：康春杨左显维韩根亮宋青陈云琳兰州交通大学数理学院兰州730070 甘肃省科学院传感技术研究所兰州730000 北京交通大学理学院微纳材料及应用研究所北京100044

采用单模微波法快速合成了红光发射的NaYF_(4):Yb^(3+)/Er^(3+)上转换纳米材料,探讨了反应温度、反应时间、反应溶剂以及掺杂离子浓度对目标产物合成的影响,并通过TEM,XRD和荧光光谱对所得样品的形貌、结构及发光性能进行了表征。结果表... 详细信息

采用单模微波法快速合成了红光发射的NaYF_(4):Yb^(3+)/Er^(3+)上转换纳米材料,探讨了反应温度、反应时间、反应溶剂以及掺杂离子浓度对目标产物合成的影响,并通过TEM,XRD和荧光光谱对所得样品的形貌、结构及发光性能进行了表征。结果表明,该方法能高效制备平均粒径为50 nm、具有立方相结构的NaYF_(4):Yb^(3+)/Er^(3+)上转换纳米材料,其在980 nm激光激发下,当Yb^(3+)和Er^(3+)掺杂浓度为20%和2%时,呈现强红光发射。通过创新发光材料的合成方法实现了强红光发射上转换纳米材料在10 min内的简单快速合成,显著提高了材料的合成效率。该研究合成的纳米材料在生物标记、细胞成像、生物检测等领域也具有巨大的应用前景。

关键词：微波法 NaYF_(4):Yb^(3+)/Er^(3+) 上转换纳米材料可控合成红色上转换发光

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An improved ileal conduit surgery for bladder cancer with fewer complications

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Cancer Communications 2019年第1期39卷 178-187页

作者： Zhiyong Li Zhuowei Liu Kai Yao Zike Qin Hui han Yonghong Li Pei Dong yunlin Ye Yanjun Wang Zhiming Wu Zhiling Zhang Fangjian Zhou Department of Urology State Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineSun Yat-sen University Cancer CenterNo.651 Dongfeng Road EastGuangzhou 510060GuangdongP.R.China

Background:Radical cystectomy and urinary diversion remains the standard surgical treatment for patients with muscle-invasive or high-risk or recurrent non-muscle-invasive bladder cancer.Although this approach prolongs patient survival remarkably,there are postoperative complications associated with urinary diversion.This study aimed to assess the efficacy of modified ileal conduit surgery for reducing early and late stoma-and ureteroileal anastomo-sis-related complications,as compared with conventional ileal conduit urinary diversion.Methods:We retrospectively evaluated the clinical data of bladder cancer patients treated with radical cystectomy and ileal conduit urinary diversion at Sun Yat-sen University Cancer Center between January 1,2000 and June 30,2016.Ileal conduit was created by the conventional or a modified technique.The clinicopathologic features of the conventional and the modified ileal conduit groups were compared using the t test and the Chi square test.Multivari-able logistic regression analysis and multivariable Cox regression analysis were performed to determine the odds of developing stoma-and ureteroileal anastomosis-related complications in the two groups.Results:145 and 100 patients underwent the modified and conventional ileal conduit surgery,respectively.The two groups were comparable with regard to clinicopathologic features.The rate of stoma-related complications was significantly lower in the modified ileal conduit group than in the conventional ileal conduit group(0.7%vs.17.0%,Phan in the conventional ileal conduit group(4.8%vs.15.0%,P=0.001).In multivariable analyses,the modified ileal conduit group was significantly less likely to develop stoma-(odds ratio[OR]=0.024,95%confidence interval[CI]0.003-0.23

关键词： Bladder cancer Cystectomy Urinary diversion Ileal conduit Complication Surgical technique

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SARS-CoV-2 infection aggravates chronic comorbidities of cardiovascular diseases and diabetes in mice

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Animal Models and Experimental Medicine 2021年第1期4卷 2-15页

作者： Yuanwu Ma Dan Lu Linlin Bao Yajin Qu Jiangning Liu Xiaolong Qi Lei Yu Xu Zhang Feifei Qi Qi Lv Yunpeng Liu Xudong Shi Caixian Sun Jing Li Jie Wang Yunlin Han Kai Gao Wei Dong Ning Liu Shan Gao Jing Xue Qiang Wei Sidan Pan Hong Gao Lianfeng Zhang Chuan Qin Key Laboratory of Human Disease Comparative Medicine National Health Commission of China(NHC)Institute of Laboratory Animal SciencePeking Union Medicine CollegeChinese Academy of Medical SciencesBeijingChina Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases Institute of Laboratory Animal SciencePeking Union Medicine CollegeChinese Academy of Medical SciencesBeijingChina

Background:Cardiovascular diseases(CVDs)and diabetes mellitus(DM)are top two chronic comorbidities that increase the severity and mortality of COVID-19.However,how SARS-CoV-2 alters the progression of chronic diseases remain unclear.Methods:We used adenovirus to deliver h-ACE2 to lung to enable SARS-CoV-2 infection in mice.SARS-CoV-2’s impacts on pathogenesis of chronic diseases were studied through histopathological,virologic and molecular biology analysis.Results:Pre-existing CVDs resulted in viral invasion,ROS elevation and activation of apoptosis pathways contribute myocardial injury during SARS-CoV-2 infection.Viral infection increased fasting blood glucose and reduced insulin response in DM model.Bone mineral density decreased shortly after infection,which associated with impaired PI3K/AKT/mTOR signaling.Conclusion:We established mouse models mimicked the complex pathological symptoms of COVID-19 patients with chronic diseases.Pre-existing diseases could impair the inflammatory responses to SARS-CoV-2 infection,which further aggravated the pre-existing diseases.This work provided valuable information to better understand the interplay between the primary diseases and SARS-CoV-2 infection.

关键词： Cardiovascular disease COVID-19 diabetes mellitus mouse model SARS-CoV-2

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计算机显微断层扫描技术(μCT)在量化阔叶树种导管中的应用

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应用生态学报 2024年第5期35卷 1214-1222页

作者：白春梅梁国芹符韵林韩尔康郭霞丽王丰广西大学林学院广西森林生态与保育重点实验室南宁530004 广西友谊关森林生态系统国家定位观测研究站广西凭祥532600 崇左凭祥友谊关森林生态系统广西野外科学观测研究站广西凭祥532600 广州市金圻睿生物科技有限责任公司广州510005 魁北克国立科学研究院水地环境中心加拿大魁北克G1K 9A9 GEOTOP地球系统动力学研究中心加拿大蒙特利尔H2X 3Y7

在细胞尺度上定量分析导管特征,对于揭示植物对环境的适应策略具有重要意义。直接打磨样品并结合体视显微镜成像技术是目前研究木质部解剖结构(针叶树管胞和阔叶树导管)的主要方法之一,但仍不可避免损伤木质部细胞,限制了对木质部细胞... 详细信息

在细胞尺度上定量分析导管特征,对于揭示植物对环境的适应策略具有重要意义。直接打磨样品并结合体视显微镜成像技术是目前研究木质部解剖结构(针叶树管胞和阔叶树导管)的主要方法之一,但仍不可避免损伤木质部细胞,限制了对木质部细胞真实解剖结构的认识。本研究选取了加拿大魁北克南部的糖枫树、北美白桦、美洲白蜡树、美洲铁木、大齿杨、苦味山核桃、北美红橡7个阔叶树种,应用计算机显微断层扫描技术(μCT)和体视显微镜法分别测量各树种的导管直径和面积,采用线性模型对两种方法的测量结果进行数据拟合,探讨采用μCT量化阔叶树导管大小的可行性。结果表明:对所测定的7个树种,应用两种方法测量导管大小的结果高度相似(R~2=0.98)。环孔材树种应用两种方法测量的导管直径结果拟合优度(苦味山核桃R~2=0.98,美洲白蜡树R~2=0.96,北美红橡R~2=0.99)高于散孔材树种(北美白桦R~2=0.88,美洲铁木R~2=0.73,糖枫树R~2=0.68,大齿杨R~2=0.88)。应用2种方法测量小导管(直径≤200μm,R~2=0.94)的结果拟合优度高于大导管(直径>200μm,R~2=0.92)。μCT技术为量化阔叶树种木质部导管提供了一种无损检测新途径。

关键词：木质部导管 Micro-CT 阔叶树

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基于顶芽智能识别的棉花化学打顶系统研究

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农业机械学报 2024年第3期55卷 145-152页

作者：韩鑫韩金鸽陈允琳兰玉彬李建坤崔立华山东理工大学农业工程与食品科学学院淄博255000 潍柴雷沃智慧农业科技股份有限公司潍坊261200 山东绿风农业集团有限公司滨州256600

设计了基于顶芽智能识别的棉花化学打顶系统,为实现精准作业,合理高效使用棉花化学打顶药剂,以减少因化学打顶剂的过度使用造成的环境污染。该系统主要由棉花顶芽识别系统、控制系统和喷施系统组成。采用YOLO v5s算法构建棉花顶芽识别... 详细信息

设计了基于顶芽智能识别的棉花化学打顶系统,为实现精准作业,合理高效使用棉花化学打顶药剂,以减少因化学打顶剂的过度使用造成的环境污染。该系统主要由棉花顶芽识别系统、控制系统和喷施系统组成。采用YOLO v5s算法构建棉花顶芽识别模型。控制系统采用STM32F407单片机,负责接收识别系统的信号,并对各个棉花打顶剂管道进行控制。同时,显示界面能够实时显示机具行驶速度、药液流量、打顶剂液位等参数。试验结果表明,在田间全天光照试验中,上午和下午时间段识别效果最优;在速度0.4 m/s下,平均识别率约为94%;信号发送区间为100 mm时,成功向下位机发送信号的成功率达到92%;田间对靶喷施试验表明,有效喷施率为94%,满足作业要求。

关键词：棉花化学打顶控制系统 YOLO v5s 顶芽识别

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CRISPR/Cas9-mediated targeting of the Rosa26 locus produces Cre reporter rat strains for monitoring Cre/loxP-mediated lineage tracing

CRISPR/Cas9-mediated targeting of the Rosa26 locus produces ...

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第十三届中国实验动物科学年会

作者： Yuanwu Ma Lei Yu Shuo Pan Shan Gao Wei Chen Xu Zhang Wei Dong Jing Li Rui Zhou Lan Huang Yunlin Han Lin Bai Li Zhang Lianfeng Zhang Key Laboratory of Human Disease Comparative Medicine NHFPC Institute of Laboratory Animal SciencePeking Union Medicine College Chinese Academy of Medical Sciences

The rat is an important laboratory animal for physiological, toxicological and pharmacological studies. CRISPR/Cas9 is a simple and efficient tool to generate precise genetic modifications in rats, which will promote the accumulation of rat genetic resources and enable more precise studies of gene function. To monitor Cre/loxP-mediated excision in vivo, we generated a Cre reporter rat strain(Rosa26-imCherry) by knockin of a Cre reporter cassette at Rosa26 locus using CRISPR/Cas9. Rosa26-imCherry rats exhibited inducible expression of the mCherry cassette(imCherry) using the Cre/loxP system, whereas normal rats exhibited ubiquitous expression of eGFP but not mCherry in the whole body. Injection of AAV9-Cre into the hippocampus and skeletal muscle resulted in mCherry expression in virus infected cells. Cre/loxP-mediated mCherry expression was then evaluated by crossing Rosa26-imCherry rats with transgenic rats ubiquitously expressing CAG-Cre, heart-specific a-MHC-Cre transgenic rats, and liver-specific Alb-Cre knockin rats. Finally, using the established system the expression pattern of Cre driven by two endogenous gene promoters(Wfs1-Cre knockin rat, Nestin-Cre knockin rat) was traced. In summary, we demonstrated excision of the loxP-flanked allele in Rosa26-imCherry rats via activation of mCherry expression in the presence of Cre recombinase. This newly established Rosa26-imCherry rat strain represents a useful tool to facilitate Cre-expression pattern determination and tracing experiments.

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