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Air Temperature and Emersion Time Can Affect the Survival Rate and Ammonium Loading of Swimming Crab Portunus trituberculatus Exposed to Air

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Journal of Ocean University of China 2020年第3期19卷 643-652页

作者： lu yunliang ZHU Boshan ZHANG Dan LI Yuquan Marine Science and Engineering College Qingdao Agricultural UniversityQingdao 266109China Key Laboratory of Mariculture of Ministry of Education Ocean University of ChinaQingdao 266003China Function Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266235China Tianjin Key Laboratory of Aqua-Ecology and Aquaculture Fisheries CollegeTianjin Agricultural UniversityTianjin 300384China

Ammonium overloading is a common response of aquatic organisms to air exposure during transport. This study elucidated the relationship between ammonium overloading and mortality of crab Portunus trituberculatus. Additionally, we also explored the effects of emersion time and air temperature on ammonium loading and concomitant physiological change. To test the air temperature effect, the crab was exposed to 16, 23 and 30℃ in air for 3 h, respectively, and then recovered in seawater at 23℃ for 12 h. To test the emersion time effect, crab was exposed to 23℃ in air for 0.5 and 3 h, respectively, and then recovered in seawater at 23℃ for 12 h. In the control group, crab was always immersed at 23℃. At each time interval(0.5, 1.5 and 3 h during air exposure and 0.5, 2, 4 and 12 h during recovery), ammonium excretion rate, level of total ammonium, total free amino acids and urea concentration in hemolymph and the hepatopancreas enzyme activity involved in detoxifying ammonium were analysed. Results showed that crab mortality was positively related with emersion time and temperature while ammonium loading was lower at 16 and 30℃ than at 23℃. For crab experiencing thermal inconsistence of culture media(i.e., 16 or 30℃), they were higher in ammonium excretion rate and activities of ammonium detoxification enzymes, which may be the reason that they had a lower ammonium loading. Prolonged emersion time(3.0 h vs. 0.5 h) increased the ammonium overloading and the activity of ammonium detoxification pathways in crab. Our results demonstrated that emersion-induced ammonium overloading may not be the main reason leading to P. trituberculatus death during air exposure and subsequent recovery. When the culture medium changed, thermal variation, compared with constant temperature, could reduce ammonium overloading in crab by elevating the activities of ammonium detoxification enzymes and ammonium excretion rate during recovery period.

关键词： Portunus trituberculatus air temperature emersion time ammonium loading

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Morphological and histological changes in the brains of turbot(Scophthalmus maximus)with gonadal development

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Acta Oceanologica Sinica 2022年第12期41卷 115-122页

作者： Chunyan Zhao Liang Chi Yongshuang Xiao Bing Li yunliang lu Yanting Cui Wenqi Wang Jun Li School of Marine Science and Engineering Qingdao Agricultural UniversityQingdao 266109China College of Veterinary Medicine Qingdao Agricultural UniversityQingdao 266109China CAS Key Laboratory of Experimental Marine Biology Center for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao 266071China Marine Biology and Biotechnology Laboratory Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

The brain plays a critical role in controlling reproduction through the hypothalamus-pituitary-gonadal(HPG)axis in ***(Scophthalmus maximus)has become an economically important marine fish in Europe and North *** research investigating turbot reproduction has focused on the role of the HPG axis in regulating egg and sperm ***,the morphology and histology of the organs in the HPG axis have not been *** this study,we investigated the morphology and histology of brains in female and male turbot at different stages of gonadal *** results showed that the brains of both female and male turbot were composed of seven parts that are typical of advanced teleosts:the telencephalon,diencephalon,cerebellum,hypothalamus,pituitary gland,myelencephalon,and olfactory *** telencephalon was well-developed and contained five distinct lobes,with the contiguous diencephalon at the caudal *** torus longitudinales and rostral torus semicircularis of the mesencephalon flattened along the dorsal surface,and the rostral corpus cerebellum was located in the dorsal *** actual total brain volume in mature males was significantly greater(plume in male turbot significantly increased(plustrate a distinct sex difference in the turbot brain during gonadal development,providing insight into their HPG axes.

关键词： turbot brain pituitary gonadal development morphology histology

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The Fire Fighters Protecting The Potala Palace

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China's Tibet 2008年第2期19卷 65-69页

作者： Jia Lijun lu yunliang

The Potala Palace is situated on the"red"mountain of Lhasa.A group of police fire fighters, unknown to most people,are actually the"guardians of the national treasures"and are living all year around inside the Potala *** they have formed a consolidated"fireproof wall"to protect this world famous cultural *** they took up residence 23 years ago, there have been no accidental fires in the palace.

关键词： The Fire Fighters Protecting The Potala Palace

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Expression and Prognostic Significance of HMGCS2 in Renal Cell Carcinoma

Expression and Prognostic Significance of HMGCS2 in Renal Ce...

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2019年中国肿瘤标志物学术大会暨第十三届肿瘤标志物青年科学家论坛

作者： lu,yunliang Zhang,Zhe Zhou,Xiaoying The first affiliated hospital of guangxi medical university Life Science Institute Guangxi Medical University

Background:Kidney cancer is a malignant tumor that originates in the urinary tubular epithelial system of the renal *** incidence is ranked 6 th in men and 8 th in *** have shown that the incidence of renal cancer in China is gradually increasing.m HMGCS2 is a rate-limiting enzyme produced by ketone *** condenses acetaminophen coenzyme A and acetamidine coenzyme A to form *** step is an irreversible key step in the formation of ketone *** to reports,HMGCS2 plays an important role in the development of various ***,the clinical significance and molecular mechanism of HMGCS2 in renal cell carcinoma(RCC) remains ***,this study aimed to investigate the role of HMGCS2 in the pathogenesis of renal *** and Methods:Renal cancer tissues and adjacent tissues were collected for immunohistochemistry(IHC) analysis to observe the expression of *** HMGCS2 expression data was downloaded from the tumor genomic map(TCGA) to analyze the clinical significance of *** analysis assessed the diagnostic and prognostic significance of HMGCS2 in IHC and TCGA *** data analysis site(Aipufu) was used to analyze the association between DNA methylation and mRNA expression of HMGCS2 in renal cell ***:Analysis of IHC and TCGA data showed a significant reduction in HMGCS2 in renal cell carcinoma *** expression of HMGCS2 is associated with tumor size,metastasis and T *** analysis showed that the up-regulation of HMGCS2 predicted a decline in overall survival of ***2 is hypermethylated in promoter DNA in KIRC,and the frequency of methylation in normal tissues is ***:HMGCS2 may be a suppressor gene in the development of renal cell carcinoma,and its expression in renal cell carcinoma is *** may be related to hypermethylation of the DNA promoter region of HMGCS2 in renal cancer,and further validation is *** addition,HMGCS2 is expected

关键词： HMGCS2 renal cell carcinoma methylation immunohistochemistry diagnosis

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赵固二矿14040下顺槽底抽巷围岩松动圈测定

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山西焦煤科技 2022年第9期46卷 16-18,53页

作者：卢运良河南焦煤能源有限公司救护大队河南焦作454150

为确定赵固二矿14040下顺槽底抽巷围岩松动圈范围,采用CXK12(A)-Z矿用本安型钻孔成像仪对巷道围岩松动圈进行测定。测定结果表明,14040下顺槽底抽巷整体围岩松动圈深度在2.8~3.8 m,瓦斯抽采孔封孔深度应大于4 m,以防止漏气现象发生,提... 详细信息

为确定赵固二矿14040下顺槽底抽巷围岩松动圈范围,采用CXK12(A)-Z矿用本安型钻孔成像仪对巷道围岩松动圈进行测定。测定结果表明,14040下顺槽底抽巷整体围岩松动圈深度在2.8~3.8 m,瓦斯抽采孔封孔深度应大于4 m,以防止漏气现象发生,提高抽采效果。对于围岩松动圈较深、抽采钻孔封孔难度大的区段,采取壁后注浆加固等措施,改善巷道围岩条件,在提升巷道支护强度的同时能够有效保证瓦斯抽采效率。

关键词：围岩松动圈松动圈范围钻孔窥视瓦斯抽采

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干露对三疣梭子蟹抗氧化和应激能力的影响

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水产学报 2014年第12期38卷 1996-2004页

作者：姜娜王芳路允良董双林中国海洋大学水产学院海水养殖教育部重点实验室

在实验室条件下,研究了干露时间(0.5、1.5和3 h)胁迫对三疣梭子蟹抗氧化能力和应激能力的影响,初步探讨了能够较灵敏地指示梭子蟹健康状况的胁迫指标。结果显示:(1)干露时间胁迫对超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量均无显著影响... 详细信息

在实验室条件下,研究了干露时间(0.5、1.5和3 h)胁迫对三疣梭子蟹抗氧化能力和应激能力的影响,初步探讨了能够较灵敏地指示梭子蟹健康状况的胁迫指标。结果显示:(1)干露时间胁迫对超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量均无显著影响(P>0.05);但干露时间显著影响肝胰脏总抗氧化能力(T-AOC)水平、热休克蛋白70(HSP70)表达量和肌肉乳酸含量(P0.05)。1.5 h干露后MDA含量在入水恢复阶段呈先升高后降低趋势,各时间点与对照组差异不显著(P>0.05);而3 h干露后入水恢复10 h时显著增加(P<0.05)。1.5 h干露后入水恢复4 h时,T-AOC水平显著高于对照组(P<0.05);3 h干露后入水恢复0.5 h时显著升高(P<0.05),2~10 h内基本恢复到对照组水平。干露时间胁迫后,乳酸含量均在入水恢复0.5 h时达到最大值;HSP70表达量均在入水恢复2~4 h内达到峰值。研究表明,相比于其他所选指标,T-AOC水平能够较敏感地反映干露时间胁迫对机体的生理状态的影响;SOD和MDA相互配合能够较灵敏地反映不同干露时长后机体在恢复阶段的免疫水平。

关键词：三疣梭子蟹干露时间胁迫入水恢复抗氧化应激

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赵固二矿回采巷道注浆锚索超前支护技术研究与应用

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现代矿业 2022年第11期38卷 224-227页

作者：卢运良刘栋河南焦煤能源有限公司救护大队焦作煤业(集团)新乡能源有限公司赵固二矿

巷道围岩稳定性直接关系到煤矿安全、高效生产,为保障巷道围岩稳定,以焦作煤田赵固二矿11012工作面为工程背景,综合利用现场调研、岩石力学试验、理论分析、工业性试验等手段,研究了回采巷道超前影响区域巷道顶板变形破坏特征,建立了上... 详细信息

巷道围岩稳定性直接关系到煤矿安全、高效生产,为保障巷道围岩稳定,以焦作煤田赵固二矿11012工作面为工程背景,综合利用现场调研、岩石力学试验、理论分析、工业性试验等手段,研究了回采巷道超前影响区域巷道顶板变形破坏特征,建立了上覆岩层-顶板-煤柱为统一体系的力学模型,得到了巷道围岩顶板拉伸、剪切破坏的力学判据。在上述分析的基础上,提出了适合本地质条件下的注浆锚索支护技术方案,应用后巷道围岩整体实现了高强全锚注一体化动态耦合控制。

关键词：回采巷道超前支护注浆锚索围岩稳定性

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反式聚异戊二烯的溶液性质——Ⅰ.粘度方程和无扰尺寸

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合成橡胶工业 1989年第3期12卷 185-188页

作者：吴运良吕在民吉林化学工业公司研究院

用粘度、渗透压、GPC和光散射法研究了反式聚异戊二烯(PIP)的溶液性质。分别由M_w和M_n建立了PIP在甲苯中的单分散粘度-分子量关系式。对^(0.5)/M^(0.5),用Stockmayer和 Fixman经验式计算为 0.097 nm;用 Krigbaum半经验式则为 0.096 nm... 详细信息

用粘度、渗透压、GPC和光散射法研究了反式聚异戊二烯(PIP)的溶液性质。分别由M_w和M_n建立了PIP在甲苯中的单分散粘度-分子量关系式。对^(0.5)/M^(0.5),用Stockmayer和 Fixman经验式计算为 0.097 nm;用 Krigbaum半经验式则为 0.096 nm,与文献值(0.091—0.097 nm)相当吻合。由 Krigbaum半经验式求出了[η]θ,得到了θ溶液中单分散的粘度方程。K_0 为 0.225和 0.238,与文献值 0.232和 0.191近似。

关键词：聚异戊二烯溶液粘度方程 GPC

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